• Preventive Nutrition and Food Science
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• v.19 no.3
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• pp.129-135
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• 2014

The protective role of phloroglucinol against oxidative stress and stress-induced premature senescence (SIPS) was investigated in vitro and in cell culture. Phloroglucinol had strong and concentration-dependent radical scavenging effects against nitric oxide (NO), superoxide anions ($O_2{^-}$), and hydroxyl radicals. In this study, free radical generators were used to induce oxidative stress in LLC-PK1 renal epithelial cells. Treatment with phloroglucinol attenuated the oxidative stress induced by peroxyl radicals, NO, $O_2{^-}$, and peroxynitrite. Phloroglucinol also increased cell viability and decreased lipid peroxidation in a concentration-dependent manner. WI-38 human diploid fibroblast cells were used to investigate the protective effect of phloroglucinol against hydrogen peroxide ($H_2O_2$)-induced SIPS. Phloroglucinol treatment attenuated $H_2O_2$-induced SIPS by increasing cell viability and inhibited lipid peroxidation, suggesting that treatment with phloroglucinol should delay the aging process. The present study supports the promising role of phloroglucinol as an antioxidative agent against free radical-induced oxidative stress and SIPS.

• The Journal of Korean Medicine
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• v.25 no.1
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• pp.49-59
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• 2004

Objectives: This paper is to investigate what effects Yeonryunggobondan and Palmijihwangtang have on postponing senility in rat fibroblasts, heart-endothelial cells, mesangial cells. Methods: 1. In vitro Yeonryunggobondan and Palmijihwangtang controlled the growth of fibroblasts, heart-endothelial cells, mesangial cells, extended the PDT of them. 2. After feeding rats the drugs for 2 months, the fibroblasts, heart-endothelial cells, mesangial cells were cultured. Results: 1) In fibroblasts the PDN was incresed and the PDT was decreased at passage-1, 2 by Yeonryunggobondan and Palmijihwangtang(p<0.05). 2) In heart-endothelial cells the PDN was incresed and the PDT was decreased at passage 8 by Yeonryunggobondan and Palmijihwangtang(p<0.05). 3) In mesangial cells the PDN was increased and the PDT was decreased at passage 4 by Yeonryunggobondan, the PDN was incresed at passage 4 by Palmijihwangtang(p<0.05). Conclusions: It is concluded that both Yeonryunggobondan and Palmijihwangtang maybe be conductive to protect and delay the senescence of rat fibroblasts, heart-endothelial cells, mesangial cells.

• Journal of Plant Biotechnology
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• v.48 no.4
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• pp.228-235
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• 2021

The amino acids found in plants play important roles in protein biosynthesis, signaling processes, and stress responses, and as components in other biosynthesis pathways. Amino acid degradation helps maintain plant cells' energy states under certain carbon starvation conditions. Branched-chain amino acid transferases (BCATs) play an essential role in the metabolism of branched-chain amino acids (BCAAs) such as isoleucine, leucine and valine. In this paper, we performed genome-wide RNA-seq analysis using CsBCAT7-overexpressing Arabidopsis plants. We observed significant changes in genes related to flowering time and genes that are germination-responsive in transgenic plants. RNA-seq and RT-qPCR analyses revealed that the expression levels of some BCAA catabolic genes were upregulated in these same transgenic plants, and that this correlated with a delay in their senescence phenotype when the plants were placed in extended darkness conditions. These results suggest a connection between BCAT and the genes implicated in BCAA catabolism.

• Journal of Society of Preventive Korean Medicine
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• v.9 no.1
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• pp.49-63
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• 2005

Objectives : Ojayeonjonghwan(五子衍宗丸) is composed of Polygonum multiflorum THUNB, and some medical herbs that are known as formula of senescence delay effects. The purpose of this study is to investigate th effect of Ojayeonjonghwan on antioxidant enzyme activities, such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione preoxidase (GSH-px) in rat erythrocytes and blood plasma. Methods : Sprague-Dawley rats were divided into 3 groups, Normal group (supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Ojayeonjonghwan administered group (D-galactose 50mg/kg and Ojayeonjonghwan extracts 245.0mg/200g 1time/day for 6 weeks, OJY Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : TBARS in plasma concentration of OJY group was significantly lower than that of control group. Red blood cell(RBC) SOD activity of OJY group was significantly higher than that of control group(F=16.057, p=0.0001, ANOVA test), RBC GSH-px activity of OJY group was increased(F=4.271, p=0.034, ANOVA test). RBC catalase activities of all experimental group were not significantly different. Total lipid and triglyceride concentration in plasma of all experimental groups were not significantly different. Total cholesterol concentrations in plasma of OJY group were significantly lower than those of control group(F=4.387, p=0.032, ANOVA test). Conclusions : According to the above results, it is considered that Ojayeonjonghwan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.467-471
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• 2012

In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-$PK_1$ porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide ($O_2{^-}$)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and $O_2{^-}$. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite ($ONOO^-$) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against $ONOO^-$ itself and its precursors, NO and $O_2{^-}$. Furthermore, the aging model of hydrogen peroxide ($H_2O_2$)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens. $H_2O_2$-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.

• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.237-242
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• 2017

AT-hook proteins of plant have shown to be involved in growth and development through the modification of chromatin architecture to co-regulate transcription of genes. Recently, many genes encoding AT-hook protein have been identified and their involvement in senescence delay is investigated. In this study, soybean transgenic plants overexpressing chromatin architecture-controlling ATPG7 gene was produced by Agrobacterium-mediated transformation and investigated for the positive effect on the important agronomic traits mainly focusing on yield-related components. A total of 27 transgenic soybean plants were produced from about 400 explants. $T_1$ seeds were harvested from all transgenic plants. In the analysis of genomic DNAs from soybean transformants, ATPG7 and Bar fragments were amplified as expected, 975 bp and 408 bp in size, respectively. And also exact gene expression was confirmed by reverse transcriptase-PCR (RT-PCR) from transgenic line #6, #7 and #8. In a field evaluation of yield components of ATPG7 transgenic plants ($T_3$), higher plant height, more of pod number and greater average total seed weight were observed with statistical significance. The results of this study indicate that the introduction of ATPG7 gene in soybean may have the positive effect on yield components.

• Journal of Society of Preventive Korean Medicine
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• v.7 no.1
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• pp.29-45
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• 2003

Objectives : Suoyounsoodan(首烏延壽丹) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect The purpose of this study is to investigate the effect of Suoyounsoodan(首烏延壽丹) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat plasma and liver. Methods: Sprague-Dawley rats divided into 4 groups, Young group(8 weeks old, N-8), Aging group(18 weeks old, N-18), pathologically induced aging group(injected D-galactose 50mg/kg, 1time/day for 6 weeks, CON) and Suoyounsoodan(首烏延壽丹) administered group(D-galactose 50mg/kg and Suoyounsoodan extracts 840.0mg/kg 1time/day for 6 weeks, SOY). Rats were sacrificed and TBARS, SOD, CAT, and GSH-px were mesured in rat plasma and liver. Results: Plasma and liver TBARS concentrations of SOY group was sinificantly lower than that of control. Red blood cell(RBC) SOD activities of SOY group was increased(F=3.405, p=0.034, ANOVA test), and RBC catalase activities of all experimental groups were not significantly different. RBC GSH-px activities of SOY group was increased(F=9.261, p=0.0001, ANOVA test). Liver SOD activities of SOY group was higher than that of control(F=3.806, p=0.023, ANOVA test). Liver catalase activities of all experimental groups were not significantly different, and liver GSH-px activity of SOY group was significantly higher than that of control(F=3.572, p=0.029, ANOVA test). Conclusions: According to the above results, It is considered Suoyounsoodan is effective in inhibiting lipid peroxidation and increasing anti oxidative enzyme activities in D-galactose induced aging rat.

• Journal of Society of Preventive Korean Medicine
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• v.6 no.2
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• pp.112-127
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• 2002

Objectives: Younnyeniksoobulrodan(延年益壽不老丹) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Younnyeniksoobulrodan(延年益壽不老丹) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase (GSH-px) in rat erythrocytes and liver. Methods: Sprague-Dawley rats divided into 4 gorups, Young group(8 weeks old, N-8), Aging group(18 weeks old, N-18), pathologically induced aging gorup(injected D-galatose 50mg／kg, 1time／day for 6 weeks, CON) and Younnyeniksoobulrodan(延年益壽不老丹) administered group(D-galactose 50mg／kg and Younnyeniksoobulrodan extracts 840.0mg／kg 1time／day for 6 weeks, YIB). Rats were sacrificed and TBARS, SOD, CAT, and GSH-px were measured in rat erythrocytes and liver. Results: Plasma and liver TBARS concentrations of YIB group were significantly lower than those of control. Red blood cell(RBC) SOD activities of YIB group was increased(F=3.445, p=0.033, ANOVA test), and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of YIB group was increased(F=9.365,p=0.0001, ANOVA test). Liver SOD activities of YIB group was higher than those of control(F=4.967, p=0.008, ANOVA test). Liver catalase activities of all experimental group were not significantly different, and liver GSH-px activity of YIB group was significantly higher than that of control(F=3.846, p=0.022,ANOVA test). Conclusions: According to the above results, it is considered that Younnyeniksoobulrodan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Journal of Society of Preventive Korean Medicine
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• v.8 no.1
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• pp.75-87
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• 2004

Objectives: Rubi Fructus(fruit of Rubus coreanus Miq.) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Rubi Fructus(fruit of Rubus coreanus Miq.) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat erythrocytes and blood plasma. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Rubi Fructus (fruit of Rubus coreanus Miq.) administered group(D-galactose 50mg/kg and Rubi Fructus(fruit of Rubus coreanus Miq.) extracts 85.0mg/200g 1time/day for 6 weeks, BBJ Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : Plasma TBARS concentrations of all experimental group were not significantly different. Red blood cell(RBC) SOD activities of BBJ group was increased, and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of BBJ group was increased. Plasma total lipid concentration of BBJ group were significantly lower than those of control. Plasma triglyceride, total cholesterol and HDL-cholesterol concentrations of all experimental group were not signi ficantly different. Conclusions: According to the above results, it is considered that Rubi Fructus(fruit of Rubus coreanus Miq.) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Journal of the Korean Society of Floral Art and Design
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• no.45
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• pp.3-12
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• 2021

The 1-methylcyclopropene (1-MCP) is the gaseous ethylene action inhibitor, which delay senescence and wilting by blocking the binding of its receptors. We investigated the effects of 1-MCP with different treated duration (0h (Control), 1h, and 4h) in cut flowers of Eustoma grandiflorum 'Voyage' and Cymbidium 'Lapine Hat'. The relative water uptake increased by 14.5% than control (9.8%) and the flower diameter maintained high from 3 to 11 days in 1-MCP treatment for 4h in E. grandiflorum 'Voyage'. The relative fresh weight was averagely high in 1-MCP treatment for 4h, however, the vase life was not significantly different in both cut flowers. Since the effects of 1-MCP treatment was differently responded by plant species and cultivars, interacted studies of duration and levels of 1-MCP are needed for the pronounced effects of postharvest quality.