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http://dx.doi.org/10.7744/cnujas.2012.39.4.467

The protective effect of Perilla frutescens from ONOO--induced oxidative stress and antiaging effect under cellular system  

Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology)
Hwang, Bo Ra (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Wu, Ting Ting (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Cho, Eun Ju (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Publication Information
Korean Journal of Agricultural Science / v.39, no.4, 2012 , pp. 467-471 More about this Journal
Abstract
In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-$PK_1$ porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide ($O_2{^-}$)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and $O_2{^-}$. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite ($ONOO^-$) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against $ONOO^-$ itself and its precursors, NO and $O_2{^-}$. Furthermore, the aging model of hydrogen peroxide ($H_2O_2$)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens. $H_2O_2$-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.
Keywords
Perilla frutescens; Oxidative stress; Antiaging; Free radical; $ONOO^-$;
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