• Plant Resources
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• 제2권1호
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• pp.22-25
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• 1999

Molecular markers are useful to confirm the hybridity of F1 plant derived from cross of two homozygous parents with similar morphological traits. RAPD markers were used to test F1 hybrid plant obtained from cross of two homozygous soybean (Glycine max) parents. Fl plant for cross I was made from the mating of Hobbit87 (female) and L63-1889 (male) and Fl plant for cross II was obtained from the mating of H1053 (female) and L63-1889 (male). Selfing plant per each cross was also obtained. Among 20 Operon primers used, OPA04 and OPA09 show polymorphism between cross I and II parent. Band in size 1Kb of OPA04 and 2.1Kb of OPA09 primer was polymorphic band. This fragment identified Fl hybrid plant and selfing plant in cross I and II. Female parent Hobbit87 in cross I and H1053 in cross II has no this fragment (recessive allele). However, male parent L63-1889 and Fl hybrid plant in cross I and II has this size of polymorphic band (dominant allele). This indicated that Fl hybrid and selfing plants were detected by RAPD marker before phenotypic marker would be used to identify Fl hybridity. Amplification products of selfing plant for cross I and II were completely same to the those of female parent. When mature, flower color of Fl hybrid plant in cross I and II was purple and flower color of selfing plant in cross I and II was white. Purple flower is dominant trait. Fl hybridity was successfully detected at very early growth stage using RAPD marker. Therefore, RAPD marker can be used broadly to confirm Fl hybridity in many crops.

• Journal of Plant Biology
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• 제38권1호
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• pp.39-45
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• 1995

Glycine soja Sieb. et Zucc., a predominantly selfing annual, has been served as a reservoir of germplasm for soybean, G. max (L.) Merr., cultivar improvement. This study describes the levels and distribution of genetic variation within and among 22 Korean populations of G. soja using starch gel electrophoresis. The species maintains very similar levels of genetic variability within populations observed in most other annuals. At the population level, the mean percent of polymorphic loci (P) was 32.6%, mean number of allele per locus (A) was 1.32, and mean expected heterozygosity (He) was 0.112. In addition, total genetic diversity (HT) calculated only for polymorphic loci was 0.347. However, significant differences in allele frequencies among populations were found for all loci (P<0.001 in each case) and, on average, about 70% of the total variation in the species is common to all populations. Indirects estimate of the number of migrants per generation (Nm=0.58, calculated from mean GST) indicates that gene flow is low among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygote deficiency in most populations and at all loci. This indicates that most populations sampled may have been substructed largely due to inbreeding (predominantly selfing) and restricted gene flow, coupled with founder effect and genetic drift. Considering a high genetic divergence among populations, it is recommended that several Korean populations of the species should be preserved, especially such as populations in the eastern and southeastern Korean peninsula with high variation.

• 한국육종학회지
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• 제41권3호
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• pp.319-323
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• 2009

딸기는 근연교배가 다른 작물에 비해 상대적으로 약한 타 식성 작물이다. 딸기의 대다수 품종 육성방법은 품종간 교잡으로 부터 우수한 개체를 선발하는 선발육종을 기본으로 하여 왔다. 그러나 계통 육성방법도 우수한 유전자를 집적하기 위한 방법으로 많이 이용되어 왔었다. 따라서 딸기의 육종효율을 증진시키기 위해 자식 또는 근계교배를 통하여 조합능력이 높은 유전자형을 가진 근교계를 만들고자 형질이 우수한 품종을 대상으로 자식을 실시하였다. 이 자식계통 중 초세, 당도, 경도 등 원예적 형질이 아주 우수한 "원교 3111호"를 선발하였다. "원교 3111호"의 주요 특성을 보면 초형은 직립형이고, 초세가 매우 강하며 화아분화가 매우 빠르다. 과형은 원추형이고, 과색은 선홍색이며, 화방 당 화수는 16~17개 정도이고, 평균과중이 17.2 g이다. 과실의 당도는 11.3 $^{\circ}Bx$, 산도는 0.50%, 경도는 21.7 g/$mm^2$로 대조 품종에 비해 단산비가 상당히 높고 경도도 우수하다. 전체 수량은 대조품종과 큰 차이가 없으나 저온기 착과성이 떨어져 상품과율이 대조 품종에 비해 떨어지고, 흰가루병, 탄저병 등 내병성에 약한 경향이 있어 재배품종보다는 신품종 육성을 위한 중간모본용으로 적합하다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 춘계 학술대회지
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• pp.41-42
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• 1998

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 춘계 학술대회지
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• pp.39-40
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• 1998

• 아시안잔디학회지
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• 제9권2호
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• pp.137-157
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• 1995

Some indices of species of the Arundinella hirta communities in Cheolwon area were studied and the following results were obtained. The vegetational date was analyzed from six grass stands selected with the distance away from the DMZ in Cheolwon area. Species diversity was higher in the stand away from the DMZ than in that neat to it. All six stands are not considered to be free of disturbance. Species composition of the stands might be related to the degree of disturbance. It can be said that the destruction of the vegetation by periodical fire or cutting simplifies the species composition. In the rear district the human inter ference is supposed to be more frequent. But it is supposed to be slight in near the DMZ and that the degree of disturbance is not so destructive. This could be the explanation of high diversity in these stands.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2004년도 춘계학술대회 논문집
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• pp.309-314
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• 2004

This study focused on the introduction of damage repair for polymeric composite carbody. called selfing tech-healinique. using microcapsules loaded with the healing agent The manufacturing process for microcapsules with the healing agent was introduced and tile characteristics of microcapsules manufactured by varying with various manufacturing process variables were evaluated. The DCPD was used for the healing agent and microcapsules were made of urea-formaldehyde resin. The magnitude and the size distribution of microcapsules were measured by a particle size analyzer using laser diffraction technique. Thermal stability was investigated by using a TGA under continuous and isothermal heating conditions for the healing agent. microcapsules without the healing agent. microcapsules with the healing agent.

• 미생물학회지
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• 제13권3호
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• pp.123-137
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• 1975

Sexual reproduction of paramecia have been accomplished through conjugation between individuals which have opposite mating type substances on their cilia when they were starved. Using selfing clone in which mating takes place, I examined whether a mating type change in indicidual cells required new protein and new mRNA synthesis or not and also shether there is a precursor relationship between both of the complementary mating type substances in their synthetic pathway. I found that 1. Mating type change needs new protein(s) and new mRNA synthesis. 2. Mating type substances are synthesized sequentially from mating type XIII to XIV 3. There might be a common precursor pool from which the mating type XIII substnace is synthesized and then complementary mating type XIV is fromed by addition of small group to the mating type XIII substance.

• 식물조직배양학회지
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• 제25권2호
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• pp.131-134
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• 1998

Antisense PG 유전자 형질전판 토마토로부터 자식시켜 얻은 종자를 kanamycin 내성을 이용하여 5세대까지 분리, 육성하여 antisense PG 유전자가 안정적으로 고정된 식물체를 얻었다. 이들 식물체에는 genomic DNA gel blot 분석으로 antisense PG 유전자가 안정적으로 유전되며 northern blot 분석을 통하여 antisense RNA가 발현됨을 확인하였고, 또한 antisense RNA에 의한 endogenous PG 유전자의 발현 저해를 분석하였다. Antisense PG 유전자 형질전환 성숙 토마토내 PG 효소 활성이 비형질전환 성숙 토마토에 비하여 37-65% 수준으로 저하되었다.

• 생태와환경
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• 제50권1호
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• pp.116-125
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• 2017

극한 기후에서 수분매개자가 부족한 고산식물은 번식보장을 위해 자가수정을 겪는 것으로 믿어졌다; 그러나 반대되는 증거도 있다. 고산지역에서 융설체제는 고산식물의 개화시기와 개화기간 같은 생활사 특성을 변화시키는 기능을 한다; 그러나 국내의 고산식물에 대한 융설체제 효과는 검정된 바 없다. 본 연구에서는 소백산 정상부의 아고산식물인 모데미풀의 자웅이숙성, 교배체제, 두 개체군[이른 융설 구역과 늦은 융설 구역(각기 ESP와 LSP)]의 개화특성을 조사하였다. 모데미풀은 암술과 수술의 성숙시기가 부분적으로 겹치는 불완전 자예선숙성을 나타냈다. 교배실험의 결과 대조구와 인공타가수분 처리구가 자율 및 인공자가수분 처리구보다 꽃당 골돌 및 종자를 유의하게 더 많이 생산하였다. 취과성숙률에 근거한 자율자가 수정지수(AI)와 자가화합성지수(SI)는 각기 0.33, 0.50이었다. ESP의 융설이 LSP보다 10일 빨랐고 이에 따라 ESP와 LSP의 개화수명과 계화계절이 현저한 차이를 보였으나 두 개체군의 개화절정일은 거의 차이가 없었다. 결론적으로, 소백산 아고산지역 모데미풀은 수분매개자 서비스를 통해 거의 타가수정으로 번식하는 조건부타가수정 식물이다. 또한 기후변화로 인한 융설 시점(이른 vs. 늦은)과 후속적인 개화특성의 변동은 이미 희귀식물인 모데미풀을 더욱 위협할 가능성이 있다.