• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• v.3B no.1
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• pp.10-15
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• 2003

This paper presents the digital computer performance evaluations of the three-phase self-excited induction generator (SEIG) driven by the variable speed prime mover such as the wind turbine using the nodal admittance approach steady-state frequency domain analysis with the experimental results. The three-phase SEIG setup is implemented for small-scale rural renewable energy utilizations. The experimental performance results give a good agreement with those ones obtained from the digital computer simulation. Furthermore, a feedback closed-loop voltage regulation of the three-phase SEIG as a power conditioner which is driven by a variable speed prime mover employing the static VAR compensator (SVC) circuit composed of the thyristor phase controlled reactor (TCR) and the thyristor switched capacitor(TSC) is designed and considered herein for the wind-turbine driven the power conditioner. To validate the effectiveness of the SVC-based voltage regulator of the terminal voltage of the three-phase SEIG, an inductive load parameter disturbances in stand-alone are applied and characterized in this paper. In the stand-alone power utilization system, the terminal voltage response and thyristor triggering angle response of the TCR are plotted graphically. The simulation and the experimental results prove the effectiveness and validity of the proposed SVC which is controlled by the Pl controller in terms of fast response and high performances of the three-phase SEIG driven directly by the rural renewable energy utilization like a variable-speed prime mover.

• Journal of the Korean Society of Industry Convergence
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• v.6 no.4
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• pp.393-399
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• 2003

In this paper, a self-organizing fuzzy logic controller with hybrid structure is proposed. The structure of the proposed method is composed of a basic fuzzy logic controller and the FARMA SOC(Fuzzy Autoregressive Moving Average Self-organizing Controller). The self-organizing cntroller with hybrid structure has advantage over the FARMA controller as follows. The proposed controller improves poor performance due to the lack of I/O data to calculate predictive output. I executed some computer simulations on the regulation problem of an inverted pendulum system and compared the results of the proposed method with those of the FARMA SOC method.

• Environmental Analysis Health and Toxicology
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• v.31
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• pp.15.1-15.6
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• 2016

A number of absurdities surrounding the humidifier disinfectant (HD) incident may have occurred because 1) a judicial system operates on the underlying false assumption that the involved parties are equals in knowledge, information and resource mobilization capabilities, regardless of respective real status as company or individual; 2) there is a lack of a system that mandates a company to prevent and actively manage possible catastrophes; 3) the regulatory scheme makes companies believe that as long as they are complying with the existing regulations, they have satisfied all of their responsibilities. I believe that this issue is an opportunity to bring about changes in the judicial redress system, the system of internal management of manufacturers, and the regulatory system of the government. The following regulation amendments are needed to move towards the changes stated above. First, legislation relating to victim relief that is applicable to the HD incident must be established. Second, a risk management system must be formed within the manufacturing company and to this end an institutional environment for the system must be established within regulatory framework. Furthermore, legislation must be passed that could punish companies themselves that have caused severe damage to individuals because they had failed to take necessary actions to avoid foreseeable harm. Finally, the framework of regulation must be changed so that the company, who has the necessary information regarding the product and the component chemicals used in the product, must self-directed experiment and assessment of the safety of their own products.

• Journal of the Korean Institute of Intelligent Systems
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• v.8 no.2
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• pp.21-34
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• 1998

[n this paper, we propose a hierarchical self-organizing fuzzy logic controller to improve the performance of the FARMA(Fuzzy auto-regressive moving average) SOC(Self-organizing fuzzy logic controller) when the system parameters change. The proposed controller contains the FARMA SC)C in the lower level and has a coordinator in the higher level, which evaluates convergence. and when it senses the degradation of system performance it compensates the control input by a look-up table. The proposed controller shows good perforniance over the FARMA SOC when the system parameters change. We executed some computer simulations on the regulation problem of an inlrerted pendulum system and compared the results with those of the FARMA SOC. As a result, it ha:; been shown that the proposed controller outperformed the FARMA SOC when the changes of the system parameters occurred.

• International Journal of Stem Cells
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• v.17 no.1
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• pp.51-58
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• 2024

With the activity of intestinal stem cells and continuous turnover, the gut epithelium is one of the most dynamic tissues in animals. Due to its simple yet conserved tissue structure and enteric cell composition as well as advanced genetic and histologic techniques, Drosophila serves as a valuable model system for investigating the regulation of intestinal stem cells. The Drosophila gut epithelium is in constant contact with indigenous microbiota and encounters externally introduced "non-self" substances, including foodborne pathogens. Therefore, in addition to its role in digestion and nutrient absorption, another essential function of the gut epithelium is to control the expansion of microbes while maintaining its structural integrity, necessitating a tissue turnover process involving intestinal stem cell activity. As a result, the microbiome and pathogens serve as important factors in regulating intestinal tissue turnover. In this manuscript, I discuss crucial discoveries revealing the interaction between gut microbes and the host's innate immune system, closely associated with the regulation of intestinal stem cell proliferation and differentiation, ultimately contributing to epithelial homeostasis.

• Development and Reproduction
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• v.15 no.1
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• pp.25-30
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• 2011

Embryonic stem (ES) cells can self-renew maintaining the undifferentiated state. Self renewal requires many factors such as Oct4, Sox2, FoxD3, and Nanog. NF-${\kappa}B$ is a transcription factor involved in many biological activities. Expression and activity of NF-${\kappa}B$ increase upon differentiation of ES cells. Reportedly, Nanog protein directly binds to NF-${\kappa}B$ protein and inhibits its activity in ES cells. Here, we found a potential binding site of NF-${\kappa}B$ in the human Nanog promoter and postulated that NF-${\kappa}B$ protein may regulate expression of the Nanog gene. We used human embryonic carcinoma (EC) cells as a model system of ES cells and confirmed decrease of Nanog and increase of NF-${\kappa}B$ upon differentiation induced by retinoic acid. Although deletion analysis on the DNA fragment including NF-${\kappa}B$ binding site suggested involvement of NF-${\kappa}B$ in the negative regulation of the promoter, site-directed mutation of NF-${\kappa}B$ binding site had no effect on the Nanog promoter activity. Furthermore, no direct association of NF-${\kappa}B$ with the Nanog promoter was detected during differentiation. Therefore, we conclude that NF-${\kappa}B$ protein may not be involved in transcriptional regulation of Nanog gene expression in EC cells and possibly in ES cells.

• Clinical and Experimental Pediatrics
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• v.58 no.7
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• pp.239-244
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• 2015

The complement system is part of the innate immune response and as such defends against invading pathogens, removes immune complexes and damaged self-cells, aids organ regeneration, confers neuroprotection, and engages with the adaptive immune response via T and B cells. Complement activation can either benefit or harm the host organism; thus, the complement system must maintain a balance between activation on foreign or modified self surfaces and inhibition on intact host cells. Complement regulators are essential for maintaining this balance and are classified as soluble regulators, such as factor H, and membrane-bound regulators. Defective complement regulators can damage the host cell and result in the accumulation of immunological debris. Moreover, defective regulators are associated with several autoimmune diseases such as atypical hemolytic uremic syndrome, dense deposit disease, age-related macular degeneration, and systemic lupus erythematosus. Therefore, understanding the molecular mechanisms by which the complement system is regulated is important for the development of novel therapies for complement-associated diseases.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1716-1717
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• 2007

In this paper, a second order fuzzy sliding mode control that combines with a adaptive self-regulating technique is proposed for a nonlinear system with unknown dynamics. The chattering effect that is a representative disadvantage of the sliding mode control is avoided by using the second order sliding mode control instead of the first order sliding mode control. The proposed sub-controller is composed of the equivalent control that is approximated by an online rule regulation sheme and the hitting control that is used to constrain the states of the sub-system to maintain on the sub-sliding surface and used to guarantee the system robustness. Simulation results are presented to show the effectiveness of the proposed controller

• 한국디지털정책학회:학술대회논문집
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• 2003.12a
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• pp.403-415
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• 2003

This study is performed to examine the relationships between trust and antecedents in cyberspace. Trust is the major control system in cyberspace. Different time, different spaces, different cultures, indistinct boundaries, flexible structure, separation of information from matters, cybernetics, difficulty of control are differential characteristics between cyberspace and physical space. Providing fit information, use of effective electronic channels, previous trust management, feedback system about output, flexible culture, self-regulation, off-line meeting, providing information about tasks and roles, ability, and justice are supposed to have effects on trust in cyberspace. By electronic mediated systems, the company will have more business opportunity. But trust will be conditional factors of expanding business in cyberspace.