• Reproductive and Developmental Biology
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• 제36권1호
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• 제3B권1호
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• pp.10-15
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• 2003

This paper presents the digital computer performance evaluations of the three-phase self-excited induction generator (SEIG) driven by the variable speed prime mover such as the wind turbine using the nodal admittance approach steady-state frequency domain analysis with the experimental results. The three-phase SEIG setup is implemented for small-scale rural renewable energy utilizations. The experimental performance results give a good agreement with those ones obtained from the digital computer simulation. Furthermore, a feedback closed-loop voltage regulation of the three-phase SEIG as a power conditioner which is driven by a variable speed prime mover employing the static VAR compensator (SVC) circuit composed of the thyristor phase controlled reactor (TCR) and the thyristor switched capacitor(TSC) is designed and considered herein for the wind-turbine driven the power conditioner. To validate the effectiveness of the SVC-based voltage regulator of the terminal voltage of the three-phase SEIG, an inductive load parameter disturbances in stand-alone are applied and characterized in this paper. In the stand-alone power utilization system, the terminal voltage response and thyristor triggering angle response of the TCR are plotted graphically. The simulation and the experimental results prove the effectiveness and validity of the proposed SVC which is controlled by the Pl controller in terms of fast response and high performances of the three-phase SEIG driven directly by the rural renewable energy utilization like a variable-speed prime mover.

• 한국산업융합학회 논문집
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• 제6권4호
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• pp.393-399
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• 2003

In this paper, a self-organizing fuzzy logic controller with hybrid structure is proposed. The structure of the proposed method is composed of a basic fuzzy logic controller and the FARMA SOC(Fuzzy Autoregressive Moving Average Self-organizing Controller). The self-organizing cntroller with hybrid structure has advantage over the FARMA controller as follows. The proposed controller improves poor performance due to the lack of I/O data to calculate predictive output. I executed some computer simulations on the regulation problem of an inverted pendulum system and compared the results of the proposed method with those of the FARMA SOC method.

• Environmental Analysis Health and Toxicology
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• 제31권
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• pp.15.1-15.6
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• 2016

A number of absurdities surrounding the humidifier disinfectant (HD) incident may have occurred because 1) a judicial system operates on the underlying false assumption that the involved parties are equals in knowledge, information and resource mobilization capabilities, regardless of respective real status as company or individual; 2) there is a lack of a system that mandates a company to prevent and actively manage possible catastrophes; 3) the regulatory scheme makes companies believe that as long as they are complying with the existing regulations, they have satisfied all of their responsibilities. I believe that this issue is an opportunity to bring about changes in the judicial redress system, the system of internal management of manufacturers, and the regulatory system of the government. The following regulation amendments are needed to move towards the changes stated above. First, legislation relating to victim relief that is applicable to the HD incident must be established. Second, a risk management system must be formed within the manufacturing company and to this end an institutional environment for the system must be established within regulatory framework. Furthermore, legislation must be passed that could punish companies themselves that have caused severe damage to individuals because they had failed to take necessary actions to avoid foreseeable harm. Finally, the framework of regulation must be changed so that the company, who has the necessary information regarding the product and the component chemicals used in the product, must self-directed experiment and assessment of the safety of their own products.

• 한국지능시스템학회논문지
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• 제8권2호
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• pp.21-34
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• 1998

본 논문에서는 시스템 파라미터의 변호가 있는 경우에 수렴성이 떨어지는 FARMA 자기구성 퍼지제어기의 단점을 보완하기 위하여 수렴성을 평가하여 제어입력을 보상하는 상위 계층이 있는 자기구성 퍼지제어기를 제안한다. 제안한 방법은 하위계층에서 FARMA 자기구성퍼지제어기, 상위계층에서는 조정자가 시스템의 수렴성을 평가하여 수렴성이 저하되는 것이 감지되면 출력 오차와 오차 변화율에 따라 구성된 보상표에 의하여 보상 제어입력을 구해 더해주므로써 수렴성을 개선시키도록 하였다. 도립진자 시스템에서 시스템 파라미터의 변화가 있는 경우에 대해 컴퓨터모사한 결과 제안한 방법이 FARMA 자기구성 퍼지제어기에 비하여 응답특성이 개선됨을 알 수 있었다.

• 한국발생생물학회지:발생과생식
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• 제15권1호
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• pp.25-30
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• 2011

Embryonic stem (ES) cells can self-renew maintaining the undifferentiated state. Self renewal requires many factors such as Oct4, Sox2, FoxD3, and Nanog. NF-${\kappa}B$ is a transcription factor involved in many biological activities. Expression and activity of NF-${\kappa}B$ increase upon differentiation of ES cells. Reportedly, Nanog protein directly binds to NF-${\kappa}B$ protein and inhibits its activity in ES cells. Here, we found a potential binding site of NF-${\kappa}B$ in the human Nanog promoter and postulated that NF-${\kappa}B$ protein may regulate expression of the Nanog gene. We used human embryonic carcinoma (EC) cells as a model system of ES cells and confirmed decrease of Nanog and increase of NF-${\kappa}B$ upon differentiation induced by retinoic acid. Although deletion analysis on the DNA fragment including NF-${\kappa}B$ binding site suggested involvement of NF-${\kappa}B$ in the negative regulation of the promoter, site-directed mutation of NF-${\kappa}B$ binding site had no effect on the Nanog promoter activity. Furthermore, no direct association of NF-${\kappa}B$ with the Nanog promoter was detected during differentiation. Therefore, we conclude that NF-${\kappa}B$ protein may not be involved in transcriptional regulation of Nanog gene expression in EC cells and possibly in ES cells.

• Clinical and Experimental Pediatrics
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• 제58권7호
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• pp.239-244
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• 2015

The complement system is part of the innate immune response and as such defends against invading pathogens, removes immune complexes and damaged self-cells, aids organ regeneration, confers neuroprotection, and engages with the adaptive immune response via T and B cells. Complement activation can either benefit or harm the host organism; thus, the complement system must maintain a balance between activation on foreign or modified self surfaces and inhibition on intact host cells. Complement regulators are essential for maintaining this balance and are classified as soluble regulators, such as factor H, and membrane-bound regulators. Defective complement regulators can damage the host cell and result in the accumulation of immunological debris. Moreover, defective regulators are associated with several autoimmune diseases such as atypical hemolytic uremic syndrome, dense deposit disease, age-related macular degeneration, and systemic lupus erythematosus. Therefore, understanding the molecular mechanisms by which the complement system is regulated is important for the development of novel therapies for complement-associated diseases.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.1716-1717
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• 2007

In this paper, a second order fuzzy sliding mode control that combines with a adaptive self-regulating technique is proposed for a nonlinear system with unknown dynamics. The chattering effect that is a representative disadvantage of the sliding mode control is avoided by using the second order sliding mode control instead of the first order sliding mode control. The proposed sub-controller is composed of the equivalent control that is approximated by an online rule regulation sheme and the hitting control that is used to constrain the states of the sub-system to maintain on the sub-sliding surface and used to guarantee the system robustness. Simulation results are presented to show the effectiveness of the proposed controller

• 한국디지털정책학회:학술대회논문집
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• 한국디지털정책학회 2003년도 창립학술대회
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• pp.403-415
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• 2003

This study is performed to examine the relationships between trust and antecedents in cyberspace. Trust is the major control system in cyberspace. Different time, different spaces, different cultures, indistinct boundaries, flexible structure, separation of information from matters, cybernetics, difficulty of control are differential characteristics between cyberspace and physical space. Providing fit information, use of effective electronic channels, previous trust management, feedback system about output, flexible culture, self-regulation, off-line meeting, providing information about tasks and roles, ability, and justice are supposed to have effects on trust in cyberspace. By electronic mediated systems, the company will have more business opportunity. But trust will be conditional factors of expanding business in cyberspace.

• 감성과학
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• 제26권4호
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• pp.29-40
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• 2023

불안은 주의 시스템의 균형을 깨트려 목표 지향 시스템보다 자극 주도 시스템을 우선하게 만드는 것으로 알려져 있으나, 자기 교시는 자기 조절의 효과로 목표 지향적 행동을 유도하게 한다. 본 연구는 가상현실 환경에서 현직 조종사를 대상으로 기상 및 자기 교시 조건이 조종사에게 발생하는 불안과 비행 과제의 수행에 미치는 영향을 검증하였다. 기상 조건은 시계비행 기상 상황과 악기상 상황으로 구분하였고 자기 교시의 수행 여부를 달리하여 비행 과제를 수행하게 하였다. 실험 결과 악기상 상황에서 불안과 심박수가 더 높고 비행 과제의 수행도가 더 낮은 것으로 나타났으나, 자기 교시를 수행하는 조건에서는 불안과 심박수가 더 낮고 비행 과제의 수행도가 더 높은 것으로 나타났다. 이 결과는 불안의 영향으로 비행에 어려움을 겪어 사고로 연결될 가능성이 증가할 수 있으나, 자기 교시에 의한 비행 수행의 향상으로 사고로 연결될 가능성이 감소할 수 있음을 시사한다.