• The Korean Journal of Physiology
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• 제23권1호
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• pp.89-98
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• 1989

New Zealand white 계의 토끼에게 EDC를 사용하여 hemocyanin에 접합시킨 human G-17-I을 면역한 결과 gastrin의 방사면역측정에 적합한 항 gastrin 혈청을 얻는데 성공하였다. 본 실험에서 얻은 항 gastrin 혈청의 역가는 1:25,000이며 human G-17-I과 human big gastrin에 매우 친화력이 높은 반면 다른 위장관 peptide 들과는 교차반응성이 아주 낮았다. 본 항 gastrin 혈청의 effective affinity constant는 $1.77{\times}10^{11}/M$이었다. 또한 총 결합부위(total binding sites)는 255 nM, 이질성지수(heterogeneity index)는 0.84 그리고 average affinity constant는 $0.79{\times}10^{11}/M$이었다. 본 실험에서 얻은 항 gastrin 혈청을 사용하여 사람과 흰쥐의 혈장 gastrin 농도를 방사면역측정법으로 측정한 결과 이미 잘 알려진 결과와 동일한 성적을 얻을 수 있었다. 이러한 결과들을 종합하여 볼때 본 실험에서 얻은 항 gastrin 혈청은 생리적농도로 존재하는 gastrin의 방사면역측정에 적합한 것으로 생각된다.

• 전기화학회지
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• 제3권2호
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• pp.69-71
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• 2000

카르복시산과 이황하물 다리를 가지며 산화환원 활성이 있는 칼릭스[4]아렌을 합성하였고 그것이 은과 금 표면 위에 자발적으로 흡착하는 것을 관찰하였다. 칼릭스[4]아렌은 특이한 구조 때문에 수용액에서 알칼리 토금속이온에 선택적인 친화력을 보인다 한편 둥그런 이온 수용체를 전극 위에 고정시켰을 때 금속 이온을 포획하는 것을 전압전류법 및 분광학적인 연구를 통해 확인하였다. 아울러 EDTA와 같은 강한 킬레이트제를 이용하여 이온을 가역적으로 제거할 수 있음을 보였다.

• Tribology and Lubricants
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• 제30권6호
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• pp.378-383
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• 2014

This study predicts the of the hysteretic friction of a rubber block sliding on an SMA asphalt road. The friction of filled rubber on a rough surface is primarily determined by two elements:the viscoelasticity of the rubber and the multi-scale perspective asperities of the road. The surface asperities of the substrate exert osillating forces on the rubber surface leading to energy dissipation via the internal friction of the rubber when rubber slides on a hard and rough substrate. This study defines the power spectra at different length scales by using a high-resolution surface profilometer, and uses rubber and road surface samples to conduct friction tests. I consider in detail the case when the substrate surface has a self affine fractal structure. The theory developed by Persson is applied to describe these tests through comparison with the hysteretic friction coefficient relevant to the energy dissipation of the viscoelastic rubber attributable to cyclic deformation. The results showed differences in the absolute values of predicted and measured friction, but with high correlation between these values. Hence, the friction prediction model is an appropriate tool for separating the effects of each factor. Therefore, this model will contribute to clearer understanding of the fundamental principles of rubber friction.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권6호
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• pp.413-417
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• 2000

In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium, Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1-1 $\mu\textrm{g}$/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex.

• 한국생활과학회지
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• 제18권1호
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• pp.93-112
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• 2009

The purpose of this study is to identify stresses and adjustment elements of Chinese students in Korea. The data analysis was conducted by qualitative method based on interviews with sixteen Chinese students as a focus group by the help of two interpreters. The results were as follows: first, stresses of Chinese students were from 'cultural difference', 'language', and 'financial', 'educational' and 'psychological stress', and 'university culture in Korea'. Those categories were divided into several sub categories. Second, ten helpful elements Chinese students could adapt themselves to Korean university life were such as 'Help of Korean professor and friends', 'Chinese friends' help', 'English-oriented lecture', 'Cultural affinity to Korea', 'Fluency in Korean language', 'Integration to Korean culture', 'Pride in Chinese culture', 'Individual effort', 'Satisfaction with educational environment', 'Perception of self-growth'.

• Molecules and Cells
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• 제38권3호
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• pp.195-201
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• 2015

Antibodies are powerful defense tools against pathogens but may cause autoimmune diseases when erroneously directed toward self-antigens. Thus, antibody producing cells are carefully selected, refined, and expanded in a highly regulated microenvironment (germinal center) in the peripheral lymphoid organs. A subset of T cells termed T follicular helper cells (Tfh) play a central role in instructing B cells to form a repertoire of antibody producing cells that provide life-long supply of high affinity, pathogenspecific antibodies. Therefore, understanding how Tfh cells arise and how they facilitate B cell selection and differentiation during germinal center reaction is critical to improve vaccines and better treat autoimmune diseases. In this review, I will summarise recent findings on molecular and cellular mechanisms underlying Tfh generation and function with an emphasis on T cell costimulation.

• Physical Therapy Rehabilitation Science
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• 제7권4호
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• pp.191-196
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• 2018

Objective: The purpose of this study was to investigate the effects of 5 different personalities on job stress in physical therapists. Design: Descriptive cross-sectional study. Methods: Structured self-report questionnaires consisted of demographic items, 5 types of personalities, and occupational stress. A questionnaire was distributed to 420 physical therapists working in Seoul and Incheon who had voluntarily agreed to participate in the study after the purpose of the study was explained. Of the 420 questionnaires, 405 questionnaires were collected and showed a recovery rate of 96.4%, of which 28 cases were excluded, leaving a total of 377 questionnaires being used for analyses. Pearson correlation analysis was performed to investigate the correlation of job stress and five personalities types. In addition, multiple regression analysis was performed to investigate the effect of general and occupational characteristics of physical therapist on job stress and the effect of personality type on occupational stress. All statistical significance levels were set at p<0.05. Results: The highest number of subjects, which were the physical therapists, showed the tendency to have a sincere personality, followed by affinity, openness, extroversion, and neuroticism. Physical therapists reported to be the most stressed in the order of professional role conflict, overload of work, work relation with a physician and supervisor, interpersonal relationship with patient and caregiver. The higher the level of affinity and sincerity, the lower the amount of stress received by the therapist from the interpersonal relationships between the patient and caregivers. On the other hand, the greater the level of openness and sincerity, the lower the stress levels related to professional role conflict. Conclusions: This study showed that the personality type and job stress of physiotherapists had a statistically significant effect. It is very important for physiotherapists to find their own way of coping with stress, which is satisfactory and appropriate for their job, because it is related not only to individual problems but also to the quality of patient care and medical services. Therefore, it is necessary to continue conducting research on how to relieve the stress levels of physical therapists according to their personality characteristics.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1732-1740
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• 2021

Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type TEVp is less stable under oxidized redox state. In this work, we introduced and combined C19S, C110S and C130S into TEVp variants containing T17S, L56V, N68D, I77V and S135G to improve protein solubility, and S219V to inhibit self-proteolysis. The solubility and cleavage activity of the constructed variants in Escherichia coli strains including BL21(DE3), BL21(DE3)pLys, Rossetta(DE3) and Origami(DE3) under the same induction conditions were analyzed and compared. The desirable soluble amounts, activity, and oxidative stability were identified to be reluctantly favored in the TEVp. Unlike C19S, C110S and C130S hardly impacted on decreasing protein solubility in the BL21(DE3), but they contributed to improved tolerance to the oxidative redox state in vivo and in vitro. After two fusion proteins were cleaved by purified TEVp protein containing double mutations under the oxidized redox state, the refolded disulfide-rich bovine enterokinase catalytic domain or maize peroxidase with enhanced yields were released from the regenerated amorphous cellulose via affinity absorption of the cellulose-binding module as the affinity tag.

• 공업화학
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• 제29권1호
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• pp.28-36
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• 2018

폐감귤박으로 제조한 활성탄(WCAC)을 충전한 고정층 반응기에서 아세톤, 벤젠 및 메틸메르캅탄(MM)의 3종류의 대상가스에 대한 흡착특성을 검토하였다. 단일성분계의 경우에 파과곡선으로부터 구한 파과시간은 유입농도 및 유량이 증가할수록 감소하였으나 형상비(L/D)가 증가할수록 증가하였다. WCAC에 의한 대상가스의 흡착량은 유입농도 및 형상비가 증가할수록 증가하였으나 유량증가에 따른 흡착량은 대상가스에 따라 차이를 나타내었다. 파과시간 및 흡착량 결과에 의하면 WCAC에 대한 친화력은 벤젠이 가장 높고, 다음으로 아세톤 그리고 MM의 순서이었다. 한편, 2성분계 및 3성분계 혼합가스의 흡착 경우에 파과곡선은 WCAC와 친화력이 작은 흡착질은 친화력이 큰 흡착질로 치환되면서 roll-up 현상을 보였다. 그리고 WCAC에 의한 아세톤의 흡착은 황화합물인 MM보다 비극성인 벤젠과 혼합되어 있을 경우에 영향을 크게 받는 것으로 나타났다.

• 패션비즈니스
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• 제16권3호
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• pp.45-62
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• 2012

This study aims to examine the effect of talent types on the purchasing behaviour of beauty-service products in 367 adults(female and male). The present study is the first to consider both talent types and the purchasing behaviour of beauty-service products correlates to appearance management behaviour. Frequency analysis showed that the mean of 8 talent types of 367 participants was 3.324. The highest talent type of 367 participants was interpersonal talent, the lowest talent type was logical-mathematical talent among 8 talent types. There were statistically significant relationships between the talent types(specially, bodily-kinesthetic talent and interpersonal talent) and the purchasing criteria of beauty-service products on the correlation analysis. And the effects of 8 talent types on the purchasing frequency of 4 beauty-service products were significant on the regression analysis, specifically, the results showed that the most significant among 8 talent factors was the interpersonal talent, next, the bodily-kinesthetic was. Thus, the more purchasing frequency of beauty-services products, the higher interpersonal talent were, and the better affinity with others were. As a results, the interpersonal talent factor and bodily-kinesthetic talent factor among 8 talent factors were ultimately affecting the appearance management behaviour. The higher the score, it was concluded that the subject was more active in beauty-related behavior. Conclusively, the appearance is a form of personal asset for one's self-fulfillment, and strong and beautiful appearance is a medium to improve self-esteem and dominance. It is possessing a significant influence in personal satisfaction, self-identity, and social success.