• 제어로봇시스템학회논문지
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• 제21권12호
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• pp.1109-1114
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• 2015

This paper presents an improved learning-based visual inspection method for auto parts inspection in severe lighting changes. Automobile sunroof frames are produced automatically by robots in most production lines. In the sunroof frame manufacturing process, there is a quality problem with some parts such as volts are missed. Instead of manual sampling inspection using some mechanical jig instruments, a learning-based machine vision system was proposed in the previous research[1]. But, in applying the actual sunroof frame production process, the inspection accuracy of the proposed vision system is much lowered because of severe illumination changes. In order to overcome this capricious environment, some selective feature vectors and cascade classifiers are used for each auto parts. And we are able to improve the inspection accuracy through the re-learning concept for the misclassified data. The effectiveness of the proposed visual inspection method is verified through sufficient experiments in a real sunroof production line.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권6호
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• pp.2908-2924
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• 2019

Multiple input multiple output orthogonal frequency division multiplexing (MIMO-OFDM) is widely applied in wireless communication by virtue of its excellent properties in data transmission rate and transmission accuracy. However, as a major drawback of MIMO-OFDM systems, the high peak-to-average power ratio (PAPR) complicates the design of the power amplifier at the receiver end. Some available PAPR reduction methods such as selective mapping (SLM) suffer from high computational complexity. In this paper, a low-complexity SLM method based on active constellation extension (ACE) and joint space-time selective mapping (AST-SLM) for reducing PAPR in Alamouti STBC MIMO-OFDM systems is proposed. In SLM scheme, two IFFT operations are required for obtaining each transmission sequence pair, and the selected phase vector is transmitted as side information(SI). However, in the proposed AST-SLM method, only a few IFFT operations are required for generating all the transmission sequence pairs. The complexity of AST-SLM is at least 86% less than SLM. In addition, the SI needed in AST-SLM is at least 92.1% less than SLM by using the presented blind detection scheme to estimate SI. We show, analytically and with simulations, that AST-SLM can achieve significant performance of PAPR reduction and close performance of bit error rate (BER) compared to SLM scheme.

• 대한기계학회논문집A
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• 제22권1호
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• pp.132-141
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• 1998

A parallel version of sheet forming analysis program was developed. This version is compatible with any parallel computers which support MPI that is one of the most recent and popular message passing libraries. For this purpose, SERI-SFA, a vector version which runs on Cray Y-MP C90, a sequential vector computer, was used as a source code. For the sake of the effectiveness of the work, the parallelization was focused on the selected part after checking the rank of CPU consumed from the exemplary calculation on Cray Y-MP C90. The subroutines associated with contact algorithm was selected as targe parts. For this work, MPI was used as a message passing library. For the performance verification, an oil pan and an S-rail forming simulation were carried out. The performance check was carried out by the kernel and total CPU time along with theoretical performance using Amdahl's Law. The results showed some performance improvement within the limit of the selective paralellization.

• 셀메드
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• 제4권2호
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• pp.8.1-8.6
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• 2014

Despite the remarkable advancements of the modern medicine, the traditional system of medicine (TSM) still serves as a potential primary health care modality in the in low- and middle-income countries. The recent reports suggest that there is a renewed interest has been observed towards TSM in the developed countries too, because of the adverse side-effect of modern medicines. Medicinal plants have been widely serving as a rich source of therapeutic agent. Ethiopia is one of the most reserves rich countries in the world. It is renowned for well-diversified and natural resources in terms of its unique flora and fauna. Ethiopian deep-rooted tradition and culture largely depends on the usage of plants for their religious ceremonies, impressive festivals, traditional medicinal uses and other basic necessities. The present scrutiny is an attempt to understand the omnipotent nature of an Ethiopian Ethnomedicinal plant called Tinjute [vernacular name (local native language, Amharic); Otostegia integrifolia]. There are several studies suggest that Tinjute can be used as a natural medicine or health-promoting agents for various disorders and ailments. Nevertheless, in Ethiopia, it is renowned as an insect repellent to drive-away insect vector of diseases, particularly mosquitoes in the early evening. However, there are many more issues and challenges which must be urgently addressed to scientifically formulate various potent, efficacious, safe and highly selective phytotherapeutic agents and insects' repellent from the Tinjute plant in the near future.

• Journal of Microbiology
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• 제45권3호
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• pp.213-218
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• 2007

Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium.

• 한국미생물·생명공학회지
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• 제14권2호
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• pp.125-131
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• 1986

각 효모 숙주 및 vector에 따라 lithium염 처리에 의한 생효모형질전환 최적 조건을 얻기 위하여 5종의 효모 숙주(S. cerevisiae Dl3-lA, DKD-5D, DBY-746, MC-16 및 S2022D)에 3종의 효모plasmid vector(YRp 7, YEp 13 및 YIp 5 )의 형질전환 실험과 아울러 이들 각 형질전환체내에서 도입된 plasmid들의 안정성을 조사하였다. lithium 염의 경우 LiCl가 Li-acetate에 비하여 좋은 효과를 보였으며 LiCl처리에 의한 최적 형질전환 조건은 각 숙주-vector계에 있어서 공히 균체 배양 시간은 16시간 (5.4 $\times$ $10^6$ - 2.4$\times$$10^8$cells/$m{\ell}$ ) 내외, LiCl의 농도는 0.1-0.2M, PEG(4000)의 농도는 35%, induction시간은 60분 내외 열처리는 42$^{\circ}C$에서 5분간, LiCi처리 buffer는 0.1M tris-HCl(pH 7.6)에서 가장 높은 형질전환 빈도를 보였다. 한편 Protoplast 형질전환법과 형질전환빈도를 비교해 본 결과 DKD-5 D(YEp13)과 Dl3-lA(YRp7)의 경우는 protoplast법이 DKD-5D(YRp 7), 및 DBY-746(YEp 13)에서는 LiCl처리법이 형질전환 빈도가 높았으며 MC-16(YEp 13)의 경우는 양방법에서 공히 비교적 낮은 빈도를 보였다. 각 형질전환체내에서의 도입된 plasmid의 안정성은 선택배지에서 배양했을 경우- YRp 7이 Dl3-lA 및 DKD- 5D 숙주에서 70세대후에는 80-85%가 유실되었으나 YEp13은 DKD-5D 및 DBY-746에서 35%밖에 유실되지 않았으며 MC-16숙주에서는 55% 유실로서 비교적 안정하였다. 또한 비선택배지에 배양시에는 선택배지에 배양했을 경우 보다 안정성이 다소 낮았으나 같은 경향은 보였다.

• Journal of Plant Biology
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• 제37권1호
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• pp.77-83
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• 1994

종자를 자연건조시킨 상태에서 ${\beta}-glucuronidase$ (GUS) 유전자와 hygromycin phosphotransferase (HPT) 유전자를 가진 plasmid DNA 용액을 imbibition시켰다. GUS 유전자의 경우 품종, vector의 종류, imbibition의 온도에 따라 표지유전자의 발현율에 차이가 있었으며 약 30-50%의 transient expression을 나타내었다. Hygromycin B (HmB)배지에서 선별된 개체의 genomic DNA를 뽑아 외부유전자의 존재를 dot 분석을 통하여 확인하였다. Inverse polymerase chain reaction 결과 만들어지는 생성물을 cloning하고 sequencing한 결과 CaMV35S promoter sequence를 찾았다. Hygromycin이 첨가된 배지에서 선별된 개체들에서 GUS 유전자의 primer를 이용하여 PCR를 수행한 결과 20개체 중 18개체에서 GUS 유전자가 안정되게 존재하여 HmB 배지에서 GUS 유전자의 존재비율은 90%였다. 본 연구의 결과로부터 두 개의 유전자를 소유한 pYJH vector system이 고등식물의 형질전환에 유용하게 이용될 수 있음을 알 수 있었다.

• Journal of Microbiology
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• 제40권2호
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• pp.140-145
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• 2002

The 5.8 kb pMMH1, rolling-circle replication (RCR) plasmid of the wild type soil Bacillus mesentericus was developed into a novel secretion vector system in Bacillus subtilis. The pMMHl turned out to have a replication origin and two open reading frames (ORFs) of the putative γ-GTP and type I signal peptidase (sipP). To characterize the regions necessary for plasmid stability and high copy number, five vectors (pPS, pPP, pEN, pMN, pME) were constructed by disruption or deletion of each region in pMMH1. Like pMMHl all constructed vectors were stable over 100 generations In a non-selective medium. Since pPS was the smallest (2.3 kb)of all, it was selected for the construction of a navel secretion vector, Using the $\alpha$-amylase promoter/signal sequence of B. subtilils the novel plasmid pJSN was constructed. When $\beta$-glucosidase was expressed using pJSN, we found $\beta$-glucosidase activity in the medium. This result strongly suggested that plasmid pJSN can be used for the production of bioactive peptides in B. subtilis.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.190-198
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• 2012

RNA interference (RNAi) is rapidly becoming a valuable tool in biological studies, as it allows the selective and transient knockdown of protein expression. The short-interfering RNAs (siRNAs) transiently silence gene expression. By contrast, the expressed short-hairpin RNAs induce long-term, stable knockdown of their target gene. Trichoplusia ni (T. ni) cells are widely used for mammalian cell-derived glycoprotein expression using the baculovirus system. However, a suitable shRNA expression system has not been developed yet. We investigated the potency of shRNA-mediated gene expression inhibition using human and Drosophila U6 promoters in T. ni cells. Luciferase, EGFP, and ${\beta}$-N-acetylglucosaminidase (GlcNAcase) were employed as targets to investigate knockdown of specific genes in T. ni cells. Introduction of the shRNA expression vector under the control of human U6 or Drosophila U6 promoter into T. ni cells exhibited the reduced level of luciferase, EGFP, and ${\beta}$-N-acetylglucosaminidase compared with that of untransfected cells. The shRNA was expressed and processed to siRNA in our vector-transfected T. ni cells. GlcNAcase mRNA levels were down-regulated in T. ni cells transfected with shRNA vectors-targeted GlcNAcase as compared with the control vector-treated cells. It implied that our shRNA expression vectors using human and Drosophila U6 promoters were applied in T. ni cells for the specific gene knockdown.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(4)
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• pp.179-182
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• 2000

논문은 웨이블릿(wavelet) 변환된 각 프레임의 모든 부대역의 블록들에 대해 계층적 움직임을 추정할때 고해상도 계층에서는 기저대역에서 추정된 전역 움직임 벡터를 기초로 하여 국부 움직임을 추정한다. 이때 복원 영상에 미치는 영향이 가장 큰 기저대역에 대하여 반화소를 사용하면 더욱 최적의 움직임 벡터를 추정할 수 있으나 계산량이 증가하는 단점이 있다. 블록내에 인접한 화소들 간에는 상관관계가 높다는 사실을 이용하여 오차가 최소가 되는 방향을 예측하여 선별적인 보간을 행하여 반화소 움직임을 탐색하여 계산량을 줄였다. 그리고 더욱 향상된 화질을 얻기 위해서 에지 성분이 많은 고해상도 계층에서 저해상도 계층으로의 선택적 국부 움직임을 추정하였다. 모의 실험 결과 기존의 웨이블릿 변환을 이용한 움직임 추정 및 보상 방법보다 향상된 화질을 나타내었다.