• Title/Summary/Keyword: Selective extraction

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Selective Extraction of Cytotoxic Substances from Medicinal Plants using Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 약용식물 성분의 선택적 추출)

  • Choi, Young-Hae;Park, Eun-Jung;Kim, Young-Leem;Chin, Young-Won;Jeon, Seong-Ho;Joung, Seung-Nam;Yoo, Ki-Pung;Kim, Jin-Woong
    • Korean Journal of Pharmacognosy
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    • v.30 no.1
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    • pp.59-64
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    • 1999
  • Supercritical fluid extraction (SFE) technique was applied to extract cytotoxic substances from five medicinal plants including Angelica gigas, Angelica acutiloba, Aralia cordata, Spirodela polyrhiza, Bupleurum falcatum, and Acanthopanax sessiliflorus. The cytotoxicities against P388, A549, and HL-60 cell lines were determined for the supercritical carbon dioxide extracts of five plant materials employed and were compared with those of the conventional organic solvent extracts such as n-hexane, $CHCl_{3}$, and MeOH to evaluate the SFE as an alternative method to conventional organic solvent extraction. In most cases, the SFE extracts of plant materials showed enhanced cytotoxicities when compared with those of other organic solvent extracts. In addition, the optimum temperature and pressure of SFE for extraction of the cytotoxic substances were largely affected by both the plant species and the cell lines tested. These results suggested that SFE could be an alternative to the conventional organic solvent method for the selective extraction of cytotoxic compounds from plants.

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Selective Extraction of Phospholipids from Soybeans with Mixture of Supercritical Carbondioxide and Ethanol (초임계 $CO_2$와 에탄올 혼합물에 의한 대두인지질의 선택적 추출)

  • Lee, Mi-Jin;Jeon, Young-Soo;Jeong, Noh-Hee;Jeang, Bu-Shik
    • Journal of the Korean Applied Science and Technology
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    • v.27 no.3
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    • pp.233-239
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    • 2010
  • Supercritical carbondioxide is very effective in removing oils from a variety of seed matrices, devoid of any appreciable amount of phospholipid content. However, the limited solubility of phosphalipids in supercritical carbondioxide leaves behind a potentially valuable by-product in spent seed matrix. Any phospholipid extraction process from the spent matrix must maintain the structure and the quality of phospholipids and must be compatible with the end use of the seed protein meal an animal feed or for human consumption. An initial supercritical carbondioxide extraction of soybean flakes was performed at 32 MPa and $80^{\circ}C$ to extract the oils, leaving the phospholipids in the deflatted soybean flakes, A second step was performed on the defatted soybean flake using $X_{eth}$=0.10, Varying the pressure from 175 MPa to 70.6 MPa and temperature from $60^{\circ}C$ to $80^{\circ}C$. For all supercritical carbon dioxide/ethanol mixture extractions, a fraction rich in phospholipids was obtained. The fractions extracted from defatted soybean flakes were dried and them redissolved in chloroform before HPLC-ELSD analysis. Quantitative and qualitative analysis of phospholipids on soybean seeds, defatted soybean flake, and different extracted phospholipid fractions was carried out, to ascertain the effect of extraction pressure and temperature.

Selective Extraction of Cobalt and Nickel in the Presence of Magnesium from Sulphate Solutions by Versatic Acid 10 (마그네슘이 함유된 황산용액에서 Versatic acid-10에 의한 코발트와 니켈의 선택적 추출)

  • Cho, Yeon chul;Ahn, Jae woo;Lee, Jae young
    • Resources Recycling
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    • v.29 no.4
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    • pp.51-57
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    • 2020
  • Separation of Co and Ni over Mg from the sulfuric acid solutions using Cyanex272, PC88A and Versatic acid 10 as an extractant was carried out. From the comparative studies about the extraction behavior of Co, Ni and Mg, Versatic acid 10 was superior to Cyanex272 and PC88A for the selective extraction of cobalt and nickel from the mixed solutions. About 98% of Ni and Co were extracted at equilibrium pH 7.0 and less than 5% of Mg was co-extracted by Versatic acid 10. McCabe-Thiele diagram indicated two stages requirement for Co and Ni extraction by 10% Versatic acid 10 at pH 7.0 and phase ratio (A/O) of 2.0. The loaded Co and Ni in organic phase was stripped effectively the sulfuric acid concentration above 70 g/L. 99.78% of cobalt and 98.42% of nickel were stripped.

Equlibrium and Kinetics of Metal Extraction by Amidoxime (Amidoxime에 의한 금속 추출 평형 및 추출 속도)

  • Shin, Jeong-Ho;Min, Seong-Kee;Jeong, Kap-Seop;Kim, Joo-Seok
    • Applied Chemistry for Engineering
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    • v.5 no.1
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    • pp.149-159
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    • 1994
  • The kinetics and equilibrium of metal extraction by benzamidoxime and phenylacetamidoxime-chloroform were investigated to apply amidoxime to metal extraction as chelating agent. The overall extraction constant extraction mechanism and selective extraction of copper were examined from the relation among extraction ratio, hydrogen ion concentration and extractant concentration. The experimental rate equation of copper extraction coincided with the theoretical rate equation and was expressed as $R_o=k{\overline{C}}_{HRo}(C_{Mo}/C_{Ho})^{1/2}$. The chemical species extracted was found to the type of ${\overline{CuR_2}}$.

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The Simultaneous Extraction and Determination of Trace Copper and Zinc in Solvent Extraction (용매추출법에 의한 토양중의 미량 구리와 아연원소의 동시추출 및 정량에 관한 연구)

  • 정창웅;지석주;박종안
    • Journal of Environmental Health Sciences
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    • v.21 no.3
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    • pp.87-95
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    • 1995
  • A rapid and selective co-extraction systems of copper and zinc-thiocyanate complex into various types of alkylamine for the simultaneous determination of two metal ions by atomic absorption spectrometry and ion chromatograph have been proposed. The quantitative extractions of Cu(II) and Zn(II) at 0.1 M-thiocyanate and 0.1 M-HCI were achieved with Aliquat 336-$CHCl_3$. The detection limits of Cu and Zn were 2 ppb and 0.9 ppb respectively.

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Separation of Lactoferrin from Model Whey Protein Mixture by Reverse Micelles Formed by Cationic Surfactant

  • Noh, Kyung-Hyun;Rhee, Min-Suk;Imm, Jee-Young
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.131-136
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    • 2005
  • The selective extraction behavior of lactoferrin (Lf) from whey protein mixture was examined using reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide (CDAB). The major whey proteins, including ${\beta}$-lactoglobulin, ${\alpha}$-lactalbumin and bovine serum albumin, were solubilized from aqueous phase to organic phase while Lf was recovered in the aqueous phase. The solubilization behaviors of the proteins were manipulated by the process parameters such as the pH and salt concentration of the aqueous phase and the surfactant concentration in the organic phase. Efficient forward extraction was achieved with sodium borate buffer (50 mM, pH 9) containing 50 mM KCl and organic phase containing 100 mM CDAB. Based on SDS-PAGE and densitometry, about 96% of the initial Lf remained in the aqueous phase after forward extraction. The dialyzed Lf fully maintained its bacteriostatic activity against E. coli O157:H7.

Cascade Selective Window for Fast and Accurate Object Detection

  • Zhang, Shu;Cai, Yong;Xie, Mei
    • Journal of Electrical Engineering and Technology
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    • v.10 no.3
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    • pp.1227-1232
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    • 2015
  • Several works help make sliding window object detection fast, nevertheless, computational demands remain prohibitive for numerous applications. This paper proposes a fast object detection method based on three strategies: cascade classifier, selective window search and fast feature extraction. Experimental results show that the proposed method outperforms the compared methods and achieves both high detection precision and low computation cost. Our approach runs at 17ms per frame on 640×480 images while attaining state-of-the-art accuracy.

Nonlinear shape resotration based on selective learning SOFM approach (선택적 SOFM 학습법을 사용한 비선형 형상왜곡 영상의 복원)

  • 한동훈;성효경;최흥문
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.34C no.1
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    • pp.59-64
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    • 1997
  • By using a selective learnable self-organizing feature map(SOFM) a more practical and generalized mehtod is proposed in which the effective nonlinear shape restoration is possible regardless of the existence of the distortion modelss. Nonlinear mapping relation is extracted from the distorted imate by using the proposed selective learning SOFGM which has the special property of effectively creating spatially organized internal representations and nonlinear relations of various input signals. For the exact extraction of the mapping relations between the distorted image and the original one, we define a disparity index as a proximal nmeasure of the present state to the final idealy trained state of the SOFM, and we used this index to adjust the training of the mapping relations form the weights of the SOFM. Simulations are conducted on various kinds of distorted images with or without distortion models, and the results show that the proposed method is very efficeint very efficient and practical in nonlinear shape restorations.

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Comparisons of Volatile Compounds of Pinus densiflora on Kinds of Extraction Solvent and Parts of Pinus (솔 부위 및 추출 용매를 달리한 솔향의 분석)

  • 이양봉;조지은;이미정;윤정로
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.973-979
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    • 1999
  • Volatile compounds from twigs, needles and sprouts in Pinus densiflora were extracted with n hexane, diethyl ether or ethanol for 24 hours, and the extracted compounds were separated and identified by gas chromatography and mass selective detector. The kinds and amount of volatile compounds extracted from three parts of Pinus densiflora were different in solvent extraction and the extraction by the modified Liken Nickerson apparatus. The contents of volatile compounds of twigs contained more than those of needles and sprouts, and the volatile compounds were extracted more in n hexane than the others. In the extraction with hexane, the main volatile compounds of twigs were 18.5% pinene, 14.5% limonene, 12.7% pinene and 3.2% myrcene. Sprouts were 16.8% limonene, 4.4% pinene, 4.3% pinene and 1.7% myrcene. Needles contained 14.7% pinene, 5.4% pinene, 2.2% limonene and 0.8% myrcene. The highest yield for pine aroma was shown in the extraction from pine twigs with n hexane, and in this extraction the amounts of pinene, pinene and limonene were 742 g, 1108 g and 922 g per gram sample, respectively.

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