Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Selective Extraction of Phospholipids from Soybeans with Mixture of Supercritical Carbondioxide and Ethanol

초임계 $CO_2$와 에탄올 혼합물에 의한 대두인지질의 선택적 추출

  • 이미진 (충북대학교 약학대학 약학과) ;
  • 전영수 (씨엔에이 바이오텍(주)) ;
  • 정노희 (충북대학교 공과대학 공업화학과) ;
  • 장부식 (충북대학교 약학대학 약학과)
  • Received : 2010.05.13
  • Accepted : 2010.09.14
  • Published : 2010.09.30
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Abstract

Supercritical carbondioxide is very effective in removing oils from a variety of seed matrices, devoid of any appreciable amount of phospholipid content. However, the limited solubility of phosphalipids in supercritical carbondioxide leaves behind a potentially valuable by-product in spent seed matrix. Any phospholipid extraction process from the spent matrix must maintain the structure and the quality of phospholipids and must be compatible with the end use of the seed protein meal an animal feed or for human consumption. An initial supercritical carbondioxide extraction of soybean flakes was performed at 32 MPa and $80^{\circ}C$ to extract the oils, leaving the phospholipids in the deflatted soybean flakes, A second step was performed on the defatted soybean flake using $X_{eth}$=0.10, Varying the pressure from 175 MPa to 70.6 MPa and temperature from $60^{\circ}C$ to $80^{\circ}C$. For all supercritical carbon dioxide/ethanol mixture extractions, a fraction rich in phospholipids was obtained. The fractions extracted from defatted soybean flakes were dried and them redissolved in chloroform before HPLC-ELSD analysis. Quantitative and qualitative analysis of phospholipids on soybean seeds, defatted soybean flake, and different extracted phospholipid fractions was carried out, to ascertain the effect of extraction pressure and temperature.

Keywords

References

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