This experiment was conducted in the laboratory in order to find out the relationships between the root growth and soil physical properties. The soils selected for this study were Sangju sandy loam, Yeongog loam, Hwadong silty clay loam, which have been considered to be a typical upland soils of Korea. Artificial core samples were made with various moisture contents and bulk densities. Elongation rate of pea seedling taproot and soil strength were measured respectively in these core samples. The results obtained are summarized as follows: 1. The soil strength increased with the bulk density and deceased with moisture content. 2. The correlation between root elongation and soil bulk density was significantly recognized at the same moisture content and the root elongation was influenced by the bulk density more significantly at dry condition. 3. The elongation rate of pea seedling taproot was significantly decreased by increasing the strength (Yamanaka tester and Fine probe) of the soils. 4. The soil strength of $21kg/cm^2$ in fine metal probe or 24mm in Yamanaka tester was considered to be the critical point for plant growth, which was restricting root elongation smaller than 1/4 of the maximum growth rate.
PAHs commonly found in industrial sites such as manufactured gas plants (MGP) are potentially toxic, mutagenic and carcinogenic, and thus require immediate remediation. In-situ chemical oxidation (ISCO) is known as a highly efficient technology for soil and groundwater remediation. Among the several types of oxidants utilized in ISCO, persulfate has gained significant attention in recent years. Peroxydisulfate ion (S2O82-) is a strong oxidant with very high redox potential (E0 = 2.01 V). When mixed with Fe2+, it is capable of forming the sulfate radical (SO4-·) that has an even higher redox potential (E0 = 2.6 V). In this study, the influence of various iron activators on the persulfate oxidation of PAHs in contaminated soils was investigated. Several iron sources such as ferrous sulfate (FeSO4), ferrous sulfide (FeS) and zero-valent iron (Fe(0)) were tested as a persulfate activator. Acenaphthene (ANE), dibenzofuran (DBF) and fluorene (FLE) were selected as model compounds because they were the dominant PAHs found in the field-contaminated soil collected from a MGP site. Oxidation kinetics of these PAHs in an artificially contaminated soil and the PAH-contaminated field soil were investigated. For all soils, Fe(0) was the most effective iron activator. The maximum PAHs removal rate in Fe(0)-mediated reactions was 92.7% for ANE, 83.0% for FLE, and 59.3% for DBF in the artificially contaminated soil, while the removal rate of total PAHs was 72.7% in the field-contaminated soil. To promote the iron activator effect, the effects of hydroxylamine as a reducing agent on reduction of Fe3+ to Fe2+, and EDTA and pyrophosphate as chelating agents on iron stabilization in persulfate oxidation were also investigated. As hydroxylamine and chelating agents (EDTA, pyrophosphate) dosage increased, the individual PAH removal rate in the artificially contaminated soil and the total PAHs removal rate in the field-contaminated soil increased.
Application of sludge wastes into the field may help soil fertility with physical, chemical and biological properties. Efficient use of sludge waste, however, requires an individual assessment of the waste products. A lot of experiment into the organic waste-treated soils has been done for decade. However, studies have not been carried out on the assessment of agricultural soil by Basidiomycota. This study was assessed the influence of sludge application on soil healthy in agricultural upland soils. The organic wastes selected for long-term application experiment in this study were municipal sewage sludge (MSS), industrial sewage sludge (ISS), leather processing sludge (LS), alcohol fermentation processing sludge (FS), and pig manure compost (PMC). To develop the soil healthy assesment method, soil samples were diluted by 20X with distilled water. After shaking at 200rpm for 30 minutes, the shaked sample was mixed on PDA(Potato Dextrose Agar). And sterilized at $121^{\circ}C$ for 20 minutes. Coriolus hirsutus (MKACC 50560) was inoculated on petri-dish including PDA mixed sample. After the media was incubated at $25^{\circ}C$ for five days, the mycelial growth of C. hirsutus was measured. When the mycelial growth on sample media was compared with growth on media contained PDA only, well grown media contained sample soil was assesed as healthy soil. The results suggest that the simple method by Coriolus hirsutus is a handy way to assess the healthy of waste sludge-applied upland soils.
Some of soil properties already known were selected for the determination of their effect on soil moisture characteristics. Total number of 2,808 representative samples from all over Korea with the exception of Jeju Island were investigated. 1. Available water contents were 4.7 for S, 7.7 for LS, 13.2 for SL, 17.7 for L, 19.2 for SiL, 15.9 for CL, 14.5 for SCL, 18.7 for SiCL, 17.3 for SiC, and 14.9% for C, respectively. 2. Simple regression analysis showed that field capacity and available water content were most strongly associated with sand content in coarse-textured soils, and with organic matter content in fine-textured soils, whereas permanent wilting point was closely associated with clay content. 3. Available water was strongly associated with silt content and also significantly with field capacity, but either not at all or negatively with permanent wilting point. 4. Prediction equations for available water and field capacity were drown out from known soil properties, which can be used for each textural class.
Air pollution effects on soil chemical properties, denitrifying and sulfur-reducing bacteria and lichens were examined around the Yeochun industrial estate. Soil samples were collected from mountain and/or mountain edges around the Namhae Chemical Corp., which is located at the southern edge of the estate, and sampled plots which were selected at 2km, 4km, 6km, 8km, and 10km apart from the industrial estate. The forest soils around the industrial estate could be classified into the Reddish yellow forest soil group, but soil pH was quite lower than the soils of the group. Because of the extremely low soil pH, microbial activity related to mineralization of organic matter was expected to be very low even though C/N ratio ranged from 15 to 20. As a result, soil organic matter and total nitrogen in soil were relatively high compared to the soils of the group. In general, soil chemical properties around the industrial estate up to 4km apart from the estate were significantly different from the rest(more than 6km apart from the estate) possibly due to air pollution. However, denitrifying bacteria and sulfur-reducing bacteria did not show significant differences in colony forming units by the distances from the industrial estate. By the way, lichens showed distinct differences in frequencies and coverages by the distances from the industrial estate possibly due to air pollution. The corticolous lichens showed more sensitive response to the air pollution compared to the saxicolous lichens.
This research was initiated to enhance the tilth of fine-textured soil for turf growth by incorporation of crumb rubber shredded from used tires. A specific objective was to determine the physical properties of soil mixes amended with different grade and amount of crumb rubber in soils. Two soils and three different grades(3.5, 6.5 and 9.5 mm) of crumb rubber were used. The soils selected were an Arenzville silt loam(coarse-silty, mixed, nonacid, mesic Typic Udifluvents) and a Hosmer silty clay loam(fine-silty, mixed, mesic Typic Fragiudalfs). The amount of crumb rubber mixed in soil ranged from 0 to 0.4 $g{\cdot}g^{-1}$(using 0.05 $g{\cdot}g^{-1}$) increments and 0 as a control. For each treatment, soil cores were constructed following the recommendation by the United States Golf Association Green Section Record. Results indicated that porosity of the mixes decreased as the amount of crumb rubber increased. Regardless of the grade of crumb rubber, mixes with less than 0.15 $g{\cdot}g^{-1}$ of crumb rubber in fine-textured soil could not enhance their macro-porosity and hydraulic conductivity. However, as the amendment increased over 0.15 $g{\cdot}g^{-1}$, the tilth of the mixes had improved significantly macro-porosity, hydraulic conductivity and air permeability, as compared with a control.
In this paper a back-propagation neural network model is developed to estimate the preconsolidation pressure of Korean soft soils based on 176 oedometer tests and 63 piezocone test results, which were compiled from 11 sites - western and southern parts of Korea. Only 147 data were used for the training of the neural network and 29 data, which were not used during the training phase, were used for the verification of trained network. Empirical and theoretical models were compared with the developed neural network model. A simple 4-4-9-1 multi-layered neural network has been developed. The cone tip resistance $q_T$ penetration pore pressure $u_2$, total overburden pressure $\sigma_{vo}$ and effective overburden pressure $\sigma'_{vo}$ were selected as input variables. The developed neural network model was validated by comparing the prediction results of the proposed neural network model for the new data which were not used for the training of the model with the measured preconsolidation pressures. It can also predict more precise and reliable preconsolidation pressures than the analytical and empirical model. Furthermore, it can be carefully concluded that neural network model can be used as a generalized model for prediction of preconsolidation pressure throughout Korea since developed model shows good performance for the new data which were not used in both training and testing data.
Kim, Tack-Soo;Ko, Min-Jung;Lee, Se-Weon;Han, Ji-Hee;Park, Kyung-Seok;Park, Jin-Woo
The Korean Journal of Pesticide Science
/
v.15
no.4
/
pp.495-501
/
2011
Bacterial strains were isolated from forest soils of Halla mountain, Jeju island in Korea. The soil samples were collected at each altitude of 100m from 1,000 m above sea level. Total 398 strains were isolated and tested for their physiological characteristics of antagonistic and proteolytic activities, and auxin production. Among the isolates, 172 strains were selected as antifungal strains showing antagonistic activity against at least one of 8 plant fungal pathogens (Alternaria alternata, Botrytis cinerea, Collectotrichum acutatum, Fusarium oxysporum, Phytophthora capsici, Pythium ultimum and Sclerotinia sclerotiorum). In addition 203 strains for proteolytic activity and 26 strains for auxin production were characterized for further study. Je28-4 (Rhodococcus sp.) were showed 80% of control value against tomato gray mold in vivo. Thus, it is suggested that soil bacteria isolated from forest soils of Halla mountain can be important sources of bioactive compounds for improving plant growth or promising biocontrol agents.
Wei, Yuan;Chen, ZhiPeng;Wu, FengChang;Li, JiNing;ShangGuan, YuXian;Li, FaSheng;Zeng, Qing Ru;Hou, Hong
Journal of Microbiology and Biotechnology
/
v.25
no.8
/
pp.1205-1215
/
2015
Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this study was to investigate the colonization and molecular diversity of AMF associated with the Sb accumulator ramie (Boehmeria nivea) growing in Sb-contaminated soils. Four Sb mine spoils and one adjacent reference area were selected from Xikuangshan in southern China. PCR-DGGE was used to analyze the AMF community composition in ramie roots. Morphological identification was also used to analyze the species in the rhizosphere soil of ramie. Results obtained showed that mycorrhizal symbiosis was established successfully even in the most heavily polluted sites. From the unpolluted site Ref to the highest polluted site T4, the spore numbers and AMF diversity increased at first and then decreased. Colonization increased consistently with the increasing Sb concentrations in the soil. A total of 14 species were identified by morphological analysis. From the total number of species, 4 (29%) belonged to Glomus, 2 (14%) belonged to Acaulospora, 2 (14%) belonged to Funneliformis, 1 (7%) belonged to Claroideoglomus, 1 (7%) belonged to Gigaspora, 1 (7%) belonged to Paraglomus, 1 (7%) belonging to Rhizophagus, 1 (7%) belonging to Sclervocystis, and 1 (7%) belonged to Scutellospora. Some AMF sequences were present even in the most polluted site. Morphological identification and phylogenetic analysis both revealed that most species were affiliated with Glomus, suggesting that Glomus was the dominant genus in this AMF community. This study demonstrated that ramie associated with AMF may have great potential for remediation of Sb-contaminated soils.
Phosphorus transfer from agricultural soils to surface waters is an important environmental issue. Fly ash and phospho-gypsum which are industrial by-product were investigated as a means of reducing dissolved phosphorus in arable soil. To determine the optimum mixing ratio of fly ash(FA) and phospho-gypsum(PG) for reducing dissolved reactive P(DRP) in soil, various mixture ratio of FA and PG were mixed with two soil. The DRP content and pH in soils were analysed after 3 weeks incubation under flooding condition. Although DRP content in soils was significantly decreased by FA-PG mixture compared with control, there were no significant difference among the FA and PG mixture ratio of 75:25, 50:50, and 25:75. The mixture of 75% FA and 25% PG was selected for field test. A field experiment was carried out to evaluate the reducing DRP content in paddy soil to which 0(NPK), 20(FG 20), 40(FG 40), and 60(FG 60) Mg $ha^{-1}$ of the mixture were applied. The DRP content was reduced by 31% at the application rate of 60 Mg $ha^{-1}$. In contrast to deceasing DRP, Ca-P content increased significantly with the mixture application rate. After rice harvesting, available $SiO_2$, P, and exchangeable Ca content in soil increased significantly with application rate due to high content of Si, P, and Ca in the mixture. Mixtures of fly ash and gypsum should reduce P loss from paddy soil and increase soil fertility.
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