• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.107-112
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• 2006

Two Atractylodes species, A. japonica Koidz. ex Kitam. (AJ) and A. macrocephala Koidz (AM) were used in this study. AJ population had higher amounts of Sesquiterpenoids and stronger tolerance to root rot but less vigor of root growth than AM population. Two populations (AJ and AM) were crossed to make interspecific hybrid population. A total of 98 lines propagated clonally were selected from a cross of AJ and AM, and evaluated for contents of sesquiterpenoids, atractylon (ATLN) and atractylenolide III (AT3) using high performance liquid chromatography (HPLC), and growth characters such as plant height, stem number and root weight. HPLC profiles of the hybrids were compared with those of parent plants, and it demonstrated the production of introgression hybrid by crossing between AJ and AM. Of 98 clonal lines,10 lines were selected by 10% level based on the growth vigor and tolerance to root rot, and AJM2102-51 line showed the heaviest root weight (117.1 g/plant) among them. A total of 98 hybrid lines contained on average $0.16\;{\pm}\;0.10\;mg/g$ of $AT3,\;2.00\;{\pm}\;1.37\;mg/g$ of ATLN, and $2.16\;{\pm}\;1.40\;mg/g$ of total sesquiterpenoids, showing high coefficients of variation (above 65%). Ten lines having high contents of sesquiterpenoids were selected, and AJM2101-15 had the highest amount (9.83 mg/g) of ATLN, and showed 40.8 g/plant of root weight similar to mean value (39.9 g/plant) of hybrid lines. The result showed that the introgression of both characters of vigorous growth from AM and high sesquiterpenoids content from AJ could be possible to make new hybrid lines by crossing between AJ and AM.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.527-533
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• 2006

The purpose of this study is to improve the culturability of a indica type rice cultivar, IR 36, using DNA marker associated with the ability of plant regeneration in anther and seed culture. The varietal difference of ability of callus formation and plant regeneration was investigated in anther and seed culture of 8 rice cultivars. Three japonica rice cultivars showed to have better culturability than those of tongil and indica type genotypes. But two indica/japonica lines, 'MGRI 079' and 'MGRI 036', which were selected to have good culturability in previous study showed the highest regenerability (20%) in anther culture of 8 rice cultivars. Thirty four $BC_2F_4$ lines were selected by marker screening using RZ400 for 100 $BC_2F_4$ lines derived from a cross $'MGRI\;079/IR\;36^{^*3}'$. The frequency of callus formation of 30 $BC_2F_4$ lines was higher than those of 'IR 36' in anther culture of the selected $BC_2F_4$ lines. The ability of plant regeneration of 15 lines was higher than that of 'IR 36' in the seed culture of 34 $BC_2F_4$ lines. A promising line, $BC_2F_4-28$, was selected to have better culturability in the anther and seed culture of the $BC_2F_4$ lines. The heading date and grain shape of the $BC_2F_4-28$ was similar to 'IR 36'. In seed culture of 50 $BC_4F_3$ lines derived from a rice cross $'MGRI\;079/IR\;36^{^*5}'$, 11 lines including $BC_4F_3-3$ showed to have higher regenerability compared with 'IR 36'. The highest frequency of plant regeneration (11%) was obtained from $BC_4F_3-46$ in seed culture of the $BC_4F_3$ lines.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.5
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• pp.343-346
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• 2000

Rye breeding using F$_1$ hybrid began about 30 years ago, when cytoplasmically inherited forms of male sterility (CMS) and corresponding nuclear restorers were detected. It is very important to produce inbred lines for making hybrid lines because of strong self-incompatibility in rye. Among the 456 rye germplasms used in hybrid breeding scheme, 24 lines (5.3%) had the above 60% of self-fertility, and six lines of them were selected for their good agronomic characteristics and were used for subsequent inbreeding program. The average self-fertility of selected six lines was 78.4%, ranging from 72.2 to 99.5%. Genetic analysis for the self-fertility using $F_2$ populations showed that the segregation of self-fertile and sterile plants in F$_2$populations could be fit into 3 to 1 ratio suggesting self-fertility in rye be controlled by one major gene. The four different self-fertile lines, PI237923, 5C11, 5G5 and Florida black, had the same self-fertility gene because their F$_2$ plants showed almost the same self-fertility as their parents and showed no genetic segregation.

• Current Research on Agriculture and Life Sciences
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• v.27
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• pp.21-28
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• 2009

The purpose of this study was to improve the culturability of 'IR 36', a indica type rice cultivar using DNA marker associated with the ability of plant regeneration in anther and seed culture. The culturability of 6 rice cultivars and 2 indica/japonica lines ('MGRI 036', 'MGRI 079') were investigated in anther and seed culture. The culturability of 3 japonica rice cultivars were much higher than tongil and indica rice cultivars, and 'MGRI 036' and 'MGRI 079' has high culturability with 20% regenerability, also. 34 $BC_2F_4$ 4 lines were selected by marker screening using RZ400 among 90 $BC_2F_4$ lines derived from a cross between 'MGRI 079' and 'IR 36'. The frequency of callus formation of 10 $BC_2F_4$ lines were higher than 'IR 36' in anther culture among selected 34 $BC_2F_4$ lines. The ability of plant regeneration of 10 lines were higher than 'IR 36' in the seed culture among selected 34 $BC_2F_4$ lines. A promising line, $BC_2F_4$-28, was selected to have better culturability in the anther and seed culture among selected 34 $BC_2F_4$ lines. The heading date and grain shape of the $BC_2F_4$-28 was similar to 'IR 36'. Using the RZ400 DNA marker associated with the culturability will be useful method for improving of indica rice culticvar's culturability in rice breeding program.

• Korean journal of applied entomology
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• v.57 no.1
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• pp.7-13
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• 2018

Currently, there is no available tool that rapidly diagnoses pine wood nematode (PWN)-infected pine trees in the field. In this study, we synthesized and purified PWN Galectin, which might be an antigen specific to PWN, using the Baculovirus expression system. We used PWN Galectin as an antigen for generating 1,464 fusion hybridoma cell lines secreting monoclonal antibodies (Mabs). Among them, we selected 62 fusion hybridoma cell lines showing high reactivity to PWN Galectin. We further selected 12 fusion hybridoma cell lines showing high reactivity to the standard PWN-infected pine tree phosphate buffered saline (PBS) extract. Additionally, two fusion hybridoma cell lines showing no or extremely low reactivity were used as controls. The selected fusion hybridoma cell lines were subjected to limiting dilutions for selecting and establishing Mab-secreting cell lines showing higher reactivity to the standard PWN-infected pine tree extract than to the standard normal pine tree PBS extract. Moreover, the selected fusion hybridoma cell lines were further selected based on their higher reactivity to PWN protein extracts than to three non-pathogenic nematode protein extracts. The Mab-secreting cell lines established in this study could be used to develop rapid diagnostic tools that can be used in the field or in laboratories for detecting PWN-infected pine trees or PWN.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.152-157
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• 2009

To obtain pure lines for breeding $F_1$ varieties of Primula malacoides, 10 lines that were considered uniform in growth and flowering characteristics were selected from self-crossings of the sixth generation ($S_6$). Plant height of the selected lines ranged from 20.8 to 33.2 cm, and number of leaves ranged from 33.6 to 54.2. The selected lines flowered at 181.4 to 207.8 days after sowing. Three lines, including 'PR-03-81', flowered in less than 190 days from sowing, while six lines, including 'PR-03-228', only flowered after more than 200 days from sowing. In terms of petal color, six lines, including 'PR-03-81', were pink, three, including 'PR-03-236', were purple, and line 'PR-03-275' was white. Flower diameter ranged from 2.4 to 2.9 cm, and number of pedicels per plant was between 15.4 and 26.4. Number of flowers per pedicel ranged from 20.2 to 30.2. Germination was more than 70%, and number of seeds per capsule (pod) ranged from 110.0 to 162.8. In summary, plant height was low and flower diameter was small and plants had many flowers and readily formed seeds. The selected lines are not only valuable as ornamental plants but could also be used as breeding materials for $F_1$ hybrids of Primula polyantha.

• Animal Bioscience
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• v.36 no.3
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• pp.417-428
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• 2023

Objective: Few studies have genetically monitored chickens over time, and no research has been conducted on ducks. To ensure the sustainable management of key duck breeds, we used microsatellite markers to monitor Brown Tsaiya ducks over time genetically. Methods: The second, fourth, sixth to eighth generations of the Brown Tsaiya duck selected for feeding efficiency and control lines were included in this study to investigate the genetic variations, effective population size, population structure and the differentiation between populations over time with 11 microsatellite markers derived from Brown Tsaiya duck. Results: The results showed there were a slight decrease in the genetic variations and an increase in within-population inbreeding coefficient (F_IS) in both lines, but no consistent increase in F_IS was observed in each line. The effective population size in the second and eighth generations was 27.2 for the selected line and 23.9 for the control line. The change in allele richness showed a downward trend over time, and the selected line was slightly lower than the control line in each generation. The number of private alleles (N_p) in the selected line were higher than in the control line. Moderate differentiation was observed between the second and eighth generations in the selected line (F_ST = 0.0510) and the control line (F_ST = 0.0606). Overall, differentiation tended to increase with each generation, but genetic variation and structure did not change considerably after six generations in the two lines. Conclusion: This study provides a reference for poultry conservation and helps to implement cross-generation genetic monitoring and breeding plans in other duck breeds or lines to promote sustainable management.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.22 no.1
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• pp.26-34
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• 1977

27 lines were selected for the cold resistant lines from the promising lines. The cold resistant lines were chiefly selected from Oro$\times$Norin 16, Oro$\times$Norin 20 and (Yudal$\times$Kongo)$\times$Yudal combination. The varieties of lately maturing showed cold resistantce. Transplanting treatment increased cold resistantce, and early sowing and drill planting also elevated cold resistance in the direct sowing culture. Plants to be cultured on soils with optimum moisture coutent showed higher cold resistance.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.247-252
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• 2010

The present study has been performed to select the osmotic tolerant lines using polyethylene glycol (PEG 6000) through an in vitro and in vivo mutagensis with a gamma-ray. During the screening, we selected three mutant lines that seemed to confer elevated osmotic tolerance in high concentrations of PEG 6000. Fruits of these mutants (Os-HK101, Os-HK102 and Os-HK103) were increased to sugar concentration, L-glutamine acid, vitamin C content and lycopine content than those of the wild type. Also the chlorophyll contents were few decreased more in the three mutant lines than the WT plants. Our results suggest that the Os-HK101 is characterized as osmotic stress tolerance considering the sugar concentration and lycopine content. It is expected that the result of this study can be used for breeding more competitive species with respect to contents in sugar or functional chemicals from the selected osmotic resistant lines.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.341-344
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• 1997

Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.