• Title/Summary/Keyword: Selected Lines

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Isolation of Gamma-Induced Rice Mutants with Increased Tolerance to Salt by Anther Culture

  • Lee, In-Sok;Kim, Dong-Sub;Hyun, Do-Yoon;Lee, Sang-Jae;Song, Hi-Sup;Lim, Yong-Pyo;Lee, Young-Il
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.51-57
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    • 2003
  • Doubled haploids have long been recognized as a valuable tool in plant breeding since it not only offers the quickest method of advancing heterozygous breeding lines to homozygosity, but also increased the selection efficiency over conventional procedures due to better discrimination between genotypes within any one generation. Salt tolerant mutants were obtained in rice the variety, 'Hawsungbyeo', through in vitro mutagenesis of in vitro cultured anther-derived calli. Various doses (30, 50, 70 and 90 Gy) of gamma ray were applied to investigate the effect of radiation on callus formation on medium containing 1% NaCl, green plant regeneration, frequency of selected doubled haploid mutants and of the salt tolerant screen. It was demonstrated that the dose of 30 and 50 Gy gamma rays had significant effects on callus formation, regeneration and selection of salt tolerance. No tolerant lines were obtained from non-mutagenized cultures. From gamma ray irradiated cultures, five tolerant lines ($M_2$generation) at germination stage and 13 tolerant lines ($M_3$genoration) at seedling stage were obtained. The frequency of salt tolerant mutants indicates that anther culture applied in connection with gamma rays is an effective way to improve salt tolerance.

Multiple Camera Collaboration Strategies for Dynamic Object Association

  • Cho, Shung-Han;Nam, Yun-Young;Hong, Sang-Jin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.4 no.6
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    • pp.1169-1193
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    • 2010
  • In this paper, we present and compare two different multiple camera collaboration strategies to reduce false association in finding the correspondence of objects. Collaboration matrices are defined with the required minimum separation for an effective collaboration because homographic lines for objects association are ineffective with the insufficient separation. The first strategy uses the collaboration matrices to select the best pair out of many cameras having the maximum separation to efficiently collaborate on the object association. The association information in selected cameras is propagated to unselected cameras by the global information constructed from the associated targets. While the first strategy requires the long operation time to achieve the high association rate due to the limited view by the best pair, it reduces the computational cost using homographic lines. The second strategy initiates the collaboration process of objects association for all the pairing cases of cameras regardless of the separation. In each collaboration process, only crossed targets by a transformed homographic line from the other collaborating camera generate homographic lines. While the repetitive association processes improve the association performance, the transformation processes of homographic lines increase exponentially. The proposed methods are evaluated with real video sequences and compared in terms of the computational cost and the association performance. The simulation results demonstrate that the proposed methods effectively reduce the false association rate as compared with basic pair-wise collaboration.

The Design, Manufacture and Applications of a Gap Noise Generator for Testing the Characteristics of EMI from Transmission Lines (송전선로 EMI 특성 실험용 인공잡음발생장치 설계, 제작 및 적용)

  • Ju, Yun-Ro;Yang, Gwang-Ho;Myeong, Seong-Ho;Lee, Dong-Il;Sin, Gu-Yong
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.51 no.1
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    • pp.23-28
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    • 2002
  • In order to survey the radiation characteristics of pure line noise of unwanted noise from overhead high voltage AC transmission lines, a disk type gap noise generator was manufactured. Disk size which decides capacitance between the noise generator and earth was selected through preliminary indoor experiments and analysis by using surface charge method. The capacitance is one of principal parameters related to the injection of a proper noise current into lines. On the basis of the capacitance obtained from calculation, 5mm of space was given to the gap of the noise generator to be installed o test line and an aluminum disk of 60cm radius was made. The field experiments were performed with the noise generator hung on the Kochang 765 kV full scale test line. As the results, the useful data which can be used to analysis the radiation characteristics of noise from transmission lines were obtained. Those are the directivity of antenna toward the line, lateral profiles, frequency spectra, height pattern and so on.

Dynamic Line Rating Prediction in Overhead Transmission Lines Using Artificial Neural Network (신경회로망을 이용한 송전선 허용용량 예측기법)

  • Noh, Shin-Eui;Kim, Yi-Gwhan;Lim, Sung-Hun;Kim, Il-Dong
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.28 no.1
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    • pp.79-87
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    • 2014
  • With the increase of demand for electricity power, new construction and expansion of transmission lines for transport have been required. However, it has been difficult to be realized by such opposition from environmental groups and residents. Therefore, the development of techniques for effective use of existing transmission lines is more needed. In this paper, the major variables to affect the allowable transmission capacity in an overhead transmission lines were selected and the dynamic line rating (DLR) method using artificial neural networks reflecting unique environment-heat properties was proposed. To prove the proposed method, the analyzed results using the artificial neural network were compared with the ones obtained from the existing method. The analyzed results using the proposed method showed an error of 0.9% within ${\pm}$, which was to be practicable.

Effect of platform shape and damaged mooring lines on the movement of semi-submersible wind turbines (반잠수식 풍력발전기의 형상 및 계류선 손상에 따른 운동특성)

  • Dong-Uk Kwon;Hee-Chang Lim
    • Journal of Wind Energy
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    • v.13 no.3
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    • pp.72-78
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    • 2022
  • This study performed a numerical analysis on the effect of platform shape and damaged mooring lines on the movement of the semi-submersible floating offshore wind turbines (FOWT). The NREL 5 MW-OC4-DeepCwind semi-submersible wind turbine was selected as the reference model. In order to find the effect of the semi-submersible floating platform shape, the dynamic movement and feasibility of three different models were observed with and without the presence of turbine blades. In addition, extreme conditions were considered by having one of the mooring lines detached to determine the effects on the FOWT. As a result, the remaining mooring lines deviated to change the surge and sway motion, which could cause a collision with nearby marine structures, and the variation of yaw angle might lead to critical accidents such as rollover. Since the response of the floating platform after receiving mooring line damage may vary depending on the mooring pattern, the location of the mooring damage, and the direction of the wind and waves, detailed simulations showed substantial variation of damage patterns.

Varietal Classification of Introduced Forage Sorghum Germplasm for Parental Line Selection on $F_1$ Hybrid Breeding (사료용 수수 1대잡종 육성 모재 선정을 위한 도입 유전자원의 품종군 분류)

  • 강정훈;이호진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.3
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    • pp.266-273
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    • 1996
  • To obtain basic information on forage sorghum F$_1$ hybrid breeding a total of 16 lines were selected from 311 introduced sorghum germplasm accessions, assessed and classified by the taxonomic distance and principal component analysis. The lines of which plant height and morphological characters were diverse and the 50% flowering date was similar to each other, were selected for parental lines in sorghum $\times$ sweet sorghum and sorghum $\times$ sudangrass crossing groups. Three varietal groups were classified by the average linkage cluster analysis based on the D$^2$ computed in eleven characters. Group I, II and III included 6 lines of sudangrass, 4 lines of sweet sorghum and 6 lines of grain sorghum, respectively. In the result of principal component analysis for eleven characters, about 82% of total variation could be appreciated by the first four principal components, the first principal component was highly loaded with head compactness and shape, l00-seed weight, plant color and grain covering, the second principal component with flowering date, plant height and awnness.

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Identification of Nicotine Converter Plants in Burley Tobacco KB9118 (KB108)

  • Jung Suk-Hun;Chung Yun-Hwa;Keum Wan-Soo;Kang Yue-Gyu;Shin Seung-Ku;Jo Chun-Joon;Choi Sang-Ju
    • Journal of the Korean Society of Tobacco Science
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    • v.27 no.1 s.53
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    • pp.11-18
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    • 2005
  • The nicotine converter genotypes of burley tobacco (Nicotiana tabacum L.), which convert nicotine to nornicotine, contain a high amount of nornicotine that degrades tobacco quality and smoking taste. Elimination of nicotine converter plants before seed harvesting is required for breeding nicotine low-converter lines and for increasing their seed production. This study aims to develop a rapid and convenient method of identifying nicotine converter plants of burley breeding lines of KB9118(KB108) using thin-layer chromatography (TLC) and isatin coloration method. Out of 223 plants in 10 lines harvested at maturity in 2002, 102 plants ($45\%$) were identified as nicotine converters by TLC of tobacco leaves air-cured. For 16 lines selected as low-converters in 2002, 148 plants grown in the field in 2003 were tested by the isatin coloration method using two detached leaves at the flowering stage thoroughly sprayed with $1\%\;NaHCO_3$ solution and cured in conditioned chambers for the early identification of nicotine to nornicotine conversion. From these samples, 46 plants ($31\%$) in 4 lines were identified as nicotine converters, indicating that the ratio of converters significantly decreased by one time selection. Mean percent conversion of non-screened lines was $14\%$ higher than that of following generation. Therefore in the burley tobacco, a rapid and convenient means of identifying and removing nornicotine converter plants by the isatin coloration method during growth in the greenhouse or field were effective in reducing the converter plants in the following generation.

Biological and Structural Mechanisms of Disease Development and Resistance in Chili Pepper Infected with the Root-knot Nematode

  • Moon, Hyo-Sun;Khan, Zakaullah;Kim, Sang-Gyu;Son, Seon-Hye;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.26 no.2
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    • pp.149-153
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    • 2010
  • Biological and structural mechanisms of the nematode disease development in chili pepper caused by the root-knot nematode, Meloidogyne incognita, were investigated. Out of 39 chili pepper cultivars/lines tested, six were found resistant, while 33 were susceptible to M. incognita, of which a susceptible cultivar Chilseongcho and three resistant cultivar/lines CM334, 02G132 and 03G53 with different resistance degrees were selected for microscopic studies on the disease development. Gall formation was greatly reduced in the resistant cultivars/lines. Nematode penetration occurred both in the susceptible and resistant chili pepper roots; however, the penetration rates were significantly lowered in the three resistant peppers compared to the susceptible pepper cv. Chilseongcho. In the susceptible pepper, giant cells were extensively formed with no discernible necrosis around the nematode feeding sites. In the highly resistant pepper cultivar CM334, no giant cell was formed, but extensive necrosis formation was observed around the penetrating nematodes. In the other two resistant pepper lines (02G132 and 03G53), both giant cells and prominent necroses were formed, and the necrotic responses appeared to inhibit the further development of giant cells or accelerate their early degeneration. Although the nematode penetration was retarded significantly in the resistant cultivar/lines, all of the above results suggest that the disease resistance of pepper may be related to post-infectional defense mechanisms (nematode growth and development) more than pre-infectional ones (penetration and establishment). Variations in structural modifications in the resistant cultivar/lines may reflect their genetic differences related to the nematode resistance.

Characteristics of Flowering and Fruiting in New Varieties and Lines of Panax ginseng C.A. Meyer (인삼 신품종과 계통의 개화 및 결실 특성)

  • 권우생;이명구;이장호
    • Journal of Ginseng Research
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    • v.25 no.1
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    • pp.41-44
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    • 2001
  • This study aimed to identify the flowering and fruiting characteristics of 2 varieties (Chunpoong and Yunpoong), 3 superior lines (KG103, KG104, KG105) and Hwangsookjong selected for development of varieties of Panax ginseng C.A. Meyer. The flowering date of Yunpoong and KG103 line showed earlier than any other lines or variety. Fertility ratio of KG104 was higher than others and the lowest with KG103 showing over 60% in all varieties and lines tested. On the fruiting type of lines, ratio of single ovary type of Chunpoong and KG105 was higher than double ovary type while Yunpoong, KG104 and Hwangsookjong were formed more double ovary type. Seed yield of KG104 per stem was the highest but Yunpoong showed the highest per kan (90 ${\times}$ 180 cm). Seed size of KG103 was the biggest among the lines tested, meanwhile that of Chunpoong and Hwangsookjong were smaller.

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Modification of ginsenoside saponin composition via the CRISPR/Cas9-mediated knockout of protopanaxadiol 6-hydroxylase gene in Panax ginseng

  • Choi, Han Suk;Koo, Hyo Bin;Jeon, Sung Won;Han, Jung Yeon;Kim, Joung Sug;Jun, Kyong Mi;Choi, Yong Eui
    • Journal of Ginseng Research
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    • v.46 no.4
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    • pp.505-514
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    • 2022
  • Background: The roots of Panax ginseng contain two types of tetracyclic triterpenoid saponins, namely, protopanaxadiol (PPD)-type saponins and protopanaxatiol (PPT)-type saponins. In P. ginseng, the protopanaxadiol 6-hydroxylase (PPT synthase) enzyme catalyses protopanaxatriol (PPT) production from protopanaxadiol (PPD). In this study, we constructed homozygous mutant lines of ginseng by CRISPR/Cas9-mediated mutagenesis of the PPT synthase gene and obtained the mutant ginseng root lines having complete depletion of the PPT-type ginsenosides. Methods: Two sgRNAs (single guide RNAs) were designed for target mutations in the exon sequences of the two PPT synthase genes (both PPTa and PPTg sequences) with the CRISPR/Cas9 system. Transgenic ginseng roots were generated through Agrobacterium-mediated transformation. The mutant lines were screened by ginsenoside analysis and DNA sequencing. Result: Ginsenoside analysis revealed the complete depletion of PPT-type ginsenosides in three putative mutant lines (Cr4, Cr7, and Cr14). The reduction of PPT-type ginsenosides in mutant lines led to increased accumulation of PPD-type ginsenosides. The gene editing in the selected mutant lines was confirmed by targeted deep sequencing. Conclusion: We have established the genome editing protocol by CRISPR/Cas9 system in P. ginseng and demonstrated the mutated roots producing only PPD-type ginsenosides by depleting PPT-type ginsenosides. Because the pharmacological activity of PPD-group ginsenosides is significantly different from that of PPT-group ginsenosides, the new type of ginseng mutant producing only PPD-group ginsenosides may have new pharmacological characteristics compared to wild-type ginseng. This is the first report to generate target-induced mutations for the modification of saponin biosynthesis in Panax species using CRISPR-Cas9 system.