• Journal of the Institute of Electronics Engineers of Korea SP
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• v.40 no.6
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• pp.71-83
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• 2003

In this paper, a novel transcoding algorithm for the G.729A and the Selectable Mode Vocoder(SMV) vocoders via direct parameter transformation is proposed. In contrast to the conventional tandem transcoding algorithm, the proposed algorithm converts the parameters of one coder to the other without going through the decoding and encoding processes. In transcoder from SMV to G.729A, LSP conversion algorithm, pitch delay conversion algorithm and transcoding algorithm in lower rate are proposed, and in transcoder from G.729A to SMV, LSP conversion algorithm, pitch delay conversion algorithm and rate selection algorithm are proposed. Evaluation results show that while exhibiting better computational and delay characteristics, the proposed algorithm produces equivalent or Improved speech quality to that produced by the tandem transcoding algorithm.

• KSBB Journal
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• v.7 no.3
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• pp.191-200
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• 1992

Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.

• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.101-109
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• 2004

In order to enhance the productivity of AVM $B_{la}$ produced by Streptomyces avermitilis as a secondary metabolite, we established a basic protocol necessary for protoplast fusion with high-producing strains as a fusion partner, and then obtained various kinds offusants by adopting a massive strain-development procedure (a miniaturized strain screening system). An alternative fusion method using UV and/or NTG mutation of protoplasts was developed to screen genetic recombinants without specific selectable markers. In this method, the mutants obtained by protoplast fusion after UV and/or NTG treatment (95% death rate) of the respective fusion partner (protoplasts of the respective mutants resistant against L-isoleucine antimetabolites such as O-methylthreonine and/or azaleucine) were regarded as DNA-recombined protoplast fusants. Notably it was demonstrated that most of the protoplast recombinants obtained by the UV mutation method were able to biosynthesize higher amount of AVM $B_{la}$ , reaching almost three times higher level (almost equal to the industrial productivity), compared to the average AVM Bla amount of the parallel mother strains.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.8
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• pp.1503-1510
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• 2016

In this study, we implemented the emotional light controlling system by using the wheel interface built in the smart-watch devices. Most previous light controlling systems have been adopted the direct switches or smart-phone applications for presenting individual emotion in lighting systems. However, in order to control color properties, these studies have some complicated user-interfaces in systems and limitation to present various color spectrums. Therefore, we need to user-friendly interfaces and functions for controlling properties of the lightning systems such as color, tone, color temperature, brightness, and saturation in detail with the wheel interface built in the smart-watch devices. The system proposed in the study is given to choose the user's selecting the emotional status information for providing the emotional lights. The selectable emotional status such as "stable", "surprise", "tired", "angry", etc. can be among 11 kinds of emotional states. In addition, the designed system processed the user's information such as user's emotional status information, local time, location information.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1340-1345
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• 2003

In this paper, an integrated optimal design software system for structural components has been developed which interfaces existing commercial codes for CAD, CAE and Optimization. They include specialized optimal design software codes such as iSIGHT and VisualDOC, optimization module imbedded in CAD software developed by CAD developers, and optimal design software systems based on API of commercial CAD software. The advantages of the CAD imbedded optimal design approach and those of specialized optimal design software are taken to develop the system. The user defines optimal design formulation in the user interface for problem definition in the CAD control stage, where design variables are directly selectable from the CAD model and various properties and performance functions defined. The commercial CAD codes, Open I-DEAS are used for the development. The resulting software is minimally connected to CAD and CAE systems while keeping maximum independence from each other. This assures flexibility and freedom for problem definition. Fatigue life optimization is taken as a nontrivial application area. As a specific example, the shape design of a knuckle part of an automobile is performed, where the minimum fatigue life over the material domain in terms of the number of cycles of a curb strike are maximized under the constraint of not exceeding the current mass. The fatigue life has been improved by four times of the initial life. The developed software is illustrated to maintain the advantages of existing optimal design software systems while improving independency and flexibility.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.7
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• pp.1740-1746
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• 2013

Augmentative and alternative communication(AAC) is a communication tool for those with impairments or restrictions on the production or comprehension of spoken language. Traditional AAC has used a portable board with pictures and texts with which disabled person could be supported by it. Currently, smart phone based AAC researches and developments are actively studying. With emerging concepts of context-aware computing, the mobile devices can provide mobile users with timely information and location based services. We proposed a adjustable AAC system for disabled people of which has variable layouts and selectable items. We also implemented context-ware application for AAC systems including time, location and click count informations on smart phone. The system can adaptively change its layout and select AAC items depending on various situation of disabled persons.

• Journal of Plant Biotechnology
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• v.32 no.3
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• pp.161-165
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• 2005

Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.

• Archives of design research
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• v.15 no.1
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• pp.113-122
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• 2002

This thesis is focused on online education and proposes a guideline for selecting teaming theories and multimedia representation without difficulty. On the first, consideration of learning theories and analysis of multimedia properties are made, and from these results guidelines are formed. Then they are applied to each 6 types of CAI. Objectivism and constructivism could be used for the basic framework of CAI. The former is suitable for reed, sequential, structural, and passive learning style and the latter is suitable for selectable, unstructural, active, self-controled, learning style. And the quideline for selecting multimedia representation is made out of the properties of media, learners(cognitive model, proficiency, acceptance), and teaming contents. On the basis of guideline obtaining from the previous process, I suggest mosts suitable conditions for each 6 types of CAI available today. Those conditions are consist of learning theories, media selection, levels of learners, and categories and properties of teaming contents.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.161-168
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• 2004

Optimal regeneration conditions of ginseng transformants were studied. It has been known that Ferritin Light Heavy Chain (FLHC) gene remove the several heavy metal by combination, store and transport. To obtain the ginseng tolerant to heavy metal, binary vector was introduced in Agrobacterium by tri-parental mating and then Agrobacterium tumefaciens MP90/FLHC was selected on the AB media and MS media containing kanamycin. Explants were co-cultured with Agrobacterium tumefaciens MP90/FLHC, which contained NPT II as a selectable marker, tadpole ferritin heavy chain (FLHC) gene and human ferritin light chain gene and then a number of embryos were induced. The induced embryo transferred to shooting media consisting of MS medium supplemented with GA 10 mg/L. As a result of examination that induced the normal growth of transfomants, transformants showed the equivalent growth in both root and shoot on the media containing the 1/3 MS.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.407-414
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• 1997

The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.