• Title/Summary/Keyword: Seedling test

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Screening of Tomato Cultivars Resistant to Bacterial Canker by Seedling Test (유묘검정법을 이용한 궤양병 저항성 토마토품종 선발)

  • Han, You-Kyoung;Han, Kyung-Sook;Lee, Seong-Chan;Kim, Hyung-Hwan;Kim, Su;Kim, Dong-Hwi
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.290-293
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    • 2010
  • Bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis, is a very damaging disease to tomato (Lycopersicon esculentum) farm in Korea. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. Selection of resistant cultivar is the best way to prevent or reduce the occurrence of the disease. Thirty-nine tomato cultivars, twenty-one cherry tomato cultivars and thirteen rootstock tomato cultivars were inoculated with Clavibacter michiganensis subsp. michiganensis, to evaluate tomato cultivarspecific resistance against bacterial canker. In the evaluation of 73 major commercial cultivars, 'Sunmyung', 'Sweet', 'Akiko', 'Dadaki', 'Match', 'Magnet', 'Friend', and 'Greenpower' were found to have a high level of resistance to bacterial canker of tomatoes.

Germination of pelleted seeds in Rehmannia glutinosa Libosch (피막처리된 지황종자의 발아)

  • Park, Chung-Heon;Shim, Kang-Bo;Kim, Min-Kyu;Park, Chun-Geon;Seong, Nak-Sul
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.3
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    • pp.213-217
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    • 1999
  • Seed coating and pelleting techniques have been used in many crops to improve the germination vigor of tiny and light seeds. Cultivated Chinese-foxglove (Rehmannia glutlnosa) has been infected by various types of virus derived from vagetative propagation of rootstock. Seed characteristics and alginate-coated seed germination rate have been investigated to get basic information for healthy seedling production through seed propagation. Chinese-foxglove showed different numbers of seeds per pod from 61 in Seocheon local to 207 in Jiwhang 1 and 1,000grain weight also varied from 70mg of Seocheon local to 130mg of Jiwhang 1. Seeds of Chinese-foxglove has dormancy because that seed collected last year more stimulated than that of this year at germination test. Optimum alginate concentration for pellected seeds germination was 2%.

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Seed Transmission of Bipolaris coicis, B. cynodontis, B. maydis and Curvularia lunata causing Leaf Blight of Job's tears (율무에 잎마름 증상을 일으키는 Bipolaris coicis, B. cynodontis, B. maydis 및 Curvularis lunata의 종자전염)

  • Kim, Ji-Soo;Lee, Du-Hyung
    • Korean Journal Plant Pathology
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    • v.14 no.4
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    • pp.287-293
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    • 1998
  • Bipolaris coicis, B. cynodontis, B. maydis and Curvularia lunata were leaf blight fungi detected from 45 seed samples of Job's tears and B. coicis was the predominant species in seed samples followed by C. lunata, B. cynodontis and B. maydis. When the seed components were plated on test tube agar, B. coicis and C. lunata were highly detected from invelucre, glume, endosperm and stamen, but not detected from plumule. Seed infection with B. coicis casued seed rot, coleoptile blight and seedling blight of Job's tears. Conidial characteristics of leaf blight fungi were as follows; B. cynodontis was fusiform, brown, slightly curved, 0~5 distoseptate, and 16.8-48$\times$7.2-16.8${\mu}{\textrm}{m}$ in size; B. cynodontis was fusiform, brown, slightly curved, 0~8 distoseptate, and 16.8-72$\times$9.6-19.2 ${\mu}{\textrm}{m}$; B. maydis was fusiform, brown, distintly curved, 0~10 distoseptate, and 28.8-110.4$\times$12-21.6 ${\mu}{\textrm}{m}$. C. Lunata was fusiform, brown, typically curved, 0~3 distoseptate, and 7.2-24$\times$4.8-12 ${\mu}{\textrm}{m}$. B. coicis was highly pathogenic to Job's tears and corn, weakly pathogenic to rice, but not pathogenic to wheat and barley. c. lunata was highly pathogenic to Job's tears (No. 2), corn, wheat and barley, weakly pathogenic to Job's tears (No. 1), but not pathogenic to rice. All treatments were effective to inhibition of leaf blight fungi when carboram, benoram, fludioxonil, prochloraz, thioram, and tap water treated to infected seeds.

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Granulation Characteristics of Mono-granular NPK(10-0-30) Fertilizer Incorporated with Rock-Phosphate Powder and its Effects on Tobacco Plant (인광석분말을 증량제로 사용한 연초(煙草)재배용 복합비료(10-0-30)의 조립(造粒)특성 및 비효)

  • Lee, Yun-Hwan;Jeong, Hun-Chae;Kim, Yong-Yeon
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.5
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    • pp.290-295
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    • 2002
  • Fertilizer granulation test was carried out by a small pan granulator. A premixture composed of SOP 60%, urea 22% and RP powder 18% was rolled in the pan granulator while 10% phosphoric acid solution(binder) was sprayed on the rolling powder bed. Granules were developed very fast along with a little amount of binder. Hardness, brittle ratio in water and hygroscopicity of granules were improved enough to evaluate physical properties of the fertilizer. Growth responses of tobacco plant to the fertilizer were investigated at seedling and flowering stage by pot experiment under plastic film roof. Seedlings showed poor growth at nursery pot cell. In virgin soil with deficient available phosphate tobacco plant showed poor growth until budding and flowering stage but good growth in tillage soil with high cumulative phosphate.

Growth Promotion of Tomato Seedlings by Applicaion of Bacillus sp. Isolated from Rhizosphere (근권에서 분리한 Bacillus sp.의 적용에 의한 토마토의 생장 촉진)

  • Lee, Kang-Hyeong;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.279-284
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    • 2007
  • Two bacterial strains isolated from soil (Bacillus subtilis strains: PS2 and RFO41) were evaluated to determine their promoting effect on the growth of tomato seedling under axonic and pot conditions. The production of phytohormone, such as indole-3-acetic acid, indole-3-butyric acid, gibberellin and zeatin by these two strains was investigated as possible mechanisms for plant growth stimulation. Both PS2 and RFO41 were shown to produce various phytohormones, and. the production of phytohormones was stimulated by the addition of peptone-rich brain heart broth medium. In addition, these bacteria exhibited high levels of phosphatase activity, which ranged from 2.18 to $2.7\;{\mu}\;{\rho}-nitrophenol/ml/hr$. PS2 and RFO41 were applied to the pot test for growth of tomato seed with phosphate. Root and shoot lengths of germinated tomato after 15 days were 45.5% and 36.5% longer than that of control in RFO41 treated samples, respectively. Baciller sp. PS2 and RFO41 may have a potential for biofertilizer in the agriculture.

Separation and Identification of a Growth Inhibiting Compound from Aralia continentalis (독활(Aralia continentalis)로부터 생장억제물질(生長抑制物質)의 분리(分離) 및 동정(同定))

  • Kim, K.U.;Back, K.W.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.221-226
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    • 1990
  • This experiment was performed to identify and isolate a growth inhibiting compound from Aralia continentalis. In order to isolate the growth inhibiting compound from Aralia continentalis the bioassay test of lettuce seed germination and rice seedling growth were used. Through these bioassays the growth inhibiting compound which was spotted at $R_f$ 0.51 on Tlc was isolated. This compound inhibited the lettuce growth by 79% at the concentration of 1000ppm. When sprayed with $FeCl_3$ reagent, it developed a bule spot. It had UV-absorbance at 217 nm and 342 nm, and $OH^-$ of $3600cm^{-1}$, C=O of $1700cm^{-1}$, C=C of $1600cm^{-1}$, and C-O of $1200cm^{-1}$ on IR spectrum. Through HPLC analysis this compound was identified as a ferulic acid ($C_{10}H_{10}O_4$) having 25 min. retention time.

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Estimates of Early Selection Efficiency for Height Growth Using Age-Age Correlation in Pinus koraiensis (수령간 상관을 이용한 잣나무 수고 생장의 조기선발 효율 추정)

  • Han, Sang-Urk;Yi, Jae-Son
    • Journal of Korean Society of Forest Science
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    • v.84 no.3
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    • pp.356-360
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    • 1995
  • Korean white pine(Pinus koraiensis S.et Z.) is one of the important silvicultural species due to its quality timber in addition to edible pine - nut production. Selective breeding method is under progress to improve growth and nut production for this species. This study was carried out to obtain genetic information necessary for establishing advanced generation seed orchard, such as genetic parameters and efficiency of early selection. Open - pollinated cones were collected from 50 plus trees. In the spring of 1986, test plantations were established at 3 different sites with 4 - year - old seedlings. Seedling height was measured from age 5 to 10 in plantations. Genetic and phenotypic correlations based on the log of the ratio of two ages (LAK) were expressed as simple regression with statistical significance($r^2=0.7862$ and 0.8256, respectively). If 20 years is presumed to mature, the most efficient age for early selection is 3 years after planting with efficiency of 1.96.

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An Forest Ecological Environment Impact Assessment of Forest Fire Suppression Chemicals - To Plants & Soil Organism - (산불 진화용 소화약제의 산림생태환경 영향 평가 - 식물 및 토양생물독성에 대하여 -)

  • Kim, Dong-Hyun;Lee, Myung-Bo;Yoo, Se-Kuel;Na, Young-Eun;Choi, Won-Il;Kim, Eung-Sik;Jung, Ki-Chang
    • Fire Science and Engineering
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    • v.22 no.5
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    • pp.48-54
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    • 2008
  • Forest fires occur the world over, with large-scale fires constantly breaking out. A suppressant a type of forest fire chemical is widely used to respond to fires rapidly and effectively. In general, suppressants used for fires have been divided into dry powder, liquid, foam, and gel type, according to physical form and use. This study has conducted toxicity tests relating to phytotoxicity(Pinus densiflora seed germination rate and mortality of containerized seedling), and soil organism toxicity(earthworm acute toxicity tests), of these suppressants, with the loaded stream suppressant for direct forest fire extinguishing a Loaded Stream and foam concentrates generally being used in Korea. From the results of the tests, the loaded stream and the foam concentrate had an effect on the toxicity levels. In the case of the loaded stream type, it was observed that toxicity indicating a 100% lethality rate was found among all toxicity test methods. Therefore, it is determined that forest ecology environmental toxicity impact assessments related to the suppressant used to extinguish forest fires are necessary in the near future.

Antifungal Activity of Anemarrhena asphodeloides, Coptis japonica and Phellodendron amurense Extracts against Phytophthora Blight (지모, 일황련 및 황백나무 추출액의 항균활성)

  • EunSooDoh
    • Korean Journal of Plant Resources
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    • v.10 no.4
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    • pp.351-359
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    • 1997
  • Antifungal activities of the crude extracts of Anemarrhena asphodeloides. Coptis japonica and Phellodendron amurense were tested against Phvtophthora capsici. and the control effect on red-pepper phytophthora hlight and phytotoxicities of red-pepper were investigated. The results were summarized as follows; Mycelial growth and zoosporangial germination of the red-peppcr phytophthora hlight organism P. capsici were inhihited hy thc crude extracts of plant materials. Methanol extracts or plant materials had hctter antifungal activity than water extracts at hoth a room temperature and a hoiling condition. Antifungal activities of three crude extracts were gradually decreased with prolonged storage period. Red-pepper phytophthora hlight was effectively controlled hy the crude extracts of three plant materials. Of these. the crude extract of C. japonica was marvelously effective. Phytotoxic symptom to red-pepper seedling showed hy water cultural method hut not by pot test. Seed germination and radicle growth of red-pepper were inhihited hy the crude extracts of three plant materials. Phytotoxic symptoms in the leaves and fruits of red-pepper were not ohserved with exogenous foliage application of the three crude extracts.

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Establishment of Bioassay System for Developing New Insecticides I. Effects of Organic Solvents on the Toxicity against Insects, Phytotoxicity and Solubility of Compounds (살충제 개발을 위한 생물검정법의 확립 I. 각종 유기 용매가 곤충의 독성과 약해 및 화합물의 용해성에 미치는 영향)

  • 안용준;조광연
    • Korean journal of applied entomology
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    • v.31 no.2
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    • pp.182-189
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    • 1992
  • The influnces of 9 kinds of solvents on the toxicities against several insect species, phytotoxicity and solubility of compounds were evaluated by means of leaf dipping and spray methods. In case of the spray application, density and vapor pressure seemed to be a contributing factor to lethal toxicity against brown planthopper and diamond-back moth, respectively; the bigger the property of density and the smaller the vapor pressure gave the stronger toxicity. It appeared that the toxicity of solvents was not correlated with anyone of physical properties such as boiling point, dipole moment, dielectric constant, surface tension and viscosity. Spray treatment gave more toxicity to insects than leaf dipping treatment. Although dielectric constant and dipole moment seemed to be contributing factors to phytotoxic damage to rice seedling and bean plants, respectively, no general correlation between phytoxicity and the other physical properties was found. Leaf dipping application caused stronger phytotoxicity than spray application. It is concluded that 5% acetone solution may be most suitable to test chemicals because of its favorable solubility of compounds, lower toxicity to insects, and lower phytotoxicity.

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