• Journal of Gastric Cancer
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• 제1권3호
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• pp.136-143
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• 2001

Purpose: The prognosis for gastric cancer with peritoneal seeding is very poor, and the role of surgical intervention is limited. We evaluated the effect of radical removal of primary and metastatic lesions on survival in gastric cancer with peritoneal seeding. Materials and Methods: From May 1989 to March 1999 at Kosin University Gospel Hospital, 115 patients revealed gastric cancer with peritoneal seeding but without liver or lung metastasis and without follow-up loss. The study group included 86 patients who underwent surgery for radical removal of primary gastric and metastatic peritoneal lesions. The control group included 29 patients who experienced incomplete removal of primary or metastatic lesions. Both groups received intraoperative intraperitoneal chemotherapy using mytomycin or cisplatin, and 25 patients underwent postoperative intravenous chemotherapy. Results: The median survival times in the study and the control groups were 13 months and 4 months, respectively (p<0.0001). The 1-year, 2-year, and 5-year survival rates were, respectively, $50.6\%,\;18.1\%$, and $11.3\%$ in the study group and $14.8\%,\;3.7\%$ and $0\%$ in the control group (p<0.0001). In the study group, neither postoperative intravenous chemotherapy nor microscopic invasion of the resection margin had any effect on survival, but intraoperative intraperitoneal chemotherapy and degree of peri-toneal seeding, especially the amount of peritoneal seeding, had an effect on survival. In the control group, neither intraperitoneal nor intravenous chemotherapy had any effect on survival, but resection of the primary gastric lesion improved survival. Conclusion: Radical removal of primary gastric and metastatic peritoneal lesions improved the survival rate for gastric cancer with peritoneal seeding. However, a randomized prospective study is needed to correctly evaluate the effect of intraperitoneal or intravenous chemotherapy.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.129-129
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• 2022

The production area of Italian ryegrass feed is gradually increasing and labor-saving technologies are being developed. If sowing and fertilization are carried out agricultural drones, working hours and labor are reduced. The purpose of this study is to suggest an appropriate seedling amount for feed production by drone spreading of Italian ryegrass in spring. In addition, we would like to review the productivity of the drone seeding machine that is being developed by Rural Development Administration(RDA) of Korea. Italian ryegrass was sown by a drone in February at the NICS located in Gyehwa-hwa, Jeollabuk-do, South Korea. In Experiment 1, 50kg/ha, 60, 70, and 80 seeding rates were sown with a horizontal spreader drone. In Experiment 2, uniform spreaders type drone and horizontal spreader type were sown with the same seeding amount and compared. The drone was sown using the AF-52 aircraft. The higher the seeding amount, the higher the emergence rate. As the seeding amount increased, the plant length increased, but the number of tillers per individual decreased. The dry matter weight of the feed was the highest at 1,326kg/10a at the seeding rate of 70kg/ha, and decreased by 20.5% at the seeding rate of 80kg/ha. The coverage ratio was the highest at 96 at the seeding rate of 70kg/ha, which was the most advantageous for spring sowing. In the comparative experiment according to the spreader type, the uniform spreader had a high emergence rate per unit area. When the uniform spreader was used, the dry matter weight of the feed was 17% higher than that of the horizontal one, and the coverage was about 5% higher.

• 한국조경학회지
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• 제26권1호
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• pp.28-35
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• 1998

Six soil compositions with three seeding rates were evaluated for influence on germination, coverage, height and sod development of Phragmites japonica. 1. Germination was high on peat, vermiculite and bark as compared with on peatmoss and sandy loam. 2. Covering rate was high within 2 months when seeded at 9g/$m^2$, but became same within 3 months afterwards when seeded at 3,6 and 9g/$m^2$, respectively. 3. Sod was highly developed on peat and bark treatments whereas Sandy loam, peatmoss and vermiculite treatments didn't develop sod. 4. Sod grown on bark weighed light and, therefore, was suggested best from a dealing cost point of view. 5. Cutting at 10 cm height didn't influence on sod development regardless of soil compositions.

• 한국작물학회지
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• 제42권5호
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• pp.522-527
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• 1997

담수직파와 건답직파의 절충형 직파양식인 벼 요철골 담수직파방법에서 적정 파종량을 구명하고자 영남농업시험장 영덕출장소에서 1994년과 1995년 2년가 파종량 3, 4, 5, 6, 7kg/10a으로 실시한 시험결과를 요약하면 다음과 같다. 1. $m^2$당 입모수는 97~161개로 파종량이 증가할수록 입모수가 많았으나 파종량 3kg /10a에서도 요철골 담수직파재배시 안전 입모수 확보는 가능하였다. 2. 도복관연형질중 분얼결당 생중, 간벽두께 중심고비율 등은 파종량간에 큰 차이가 없었다. 파종량이 많을수록 좌절중이 떨어져 도복지수가 컸고, 포장도복은 파종량 5kg /10a이상에서 1~3정도 발생하였다. 3. 출수기는 10a당 파종량 5~7kg이 3~4kg보다 1일이 빨랐다. 4. 수량 구성요소는 파종량이 증가할수록 수수는 많았으나 등숙비율은 떨어졌고 수당 입수 및 천립중은 파종량간에 뚜렷한 차이가 없었다. 5. 건물중은 파종량 증가에 따라 증가했으나 수확지수는 떨어졌고, 2개년 평균 쌀 수량은 518~552kg /10a당 파종량 5kg /10a에서 가장 높았다. 6. 2차 회귀곡선식으로 추정한 최고수량을 나타낸 파종량은 4.3~4.8kg /10a이었고 이때 $m^2$당 입모수는 126~135개였다.

• 한국초지조사료학회지
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• 제2권1호
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• pp.31-34
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• 1980

• 한국초지조사료학회지
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• 제26권3호
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• pp.155-158
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• 2006

본 연구는 답리작에서 사일리지용 총체보리를 파종시기와 파종량에 따른 생육특성, 생초 및 건물수량에 미치는 영향을 구명하고자 수원 소재 축산연구소에서 2001년 10월부터 2003년 5월까지 3년간 수행하였다. 본 연구는 분할구 시험법으로 3반복 설계 배치하였으며, 결과를 요약하면 다음과 같다. 총체보리의 초장은 일찍 파종하는 것이 높게 나타났으며, 건물률에 있어서도 일찍 파종할수록 높았다. 즉, 10월 5일과 20일 그리고 11월 5일에 파종했을 때 각각 47.4, 41.5 그리고 34.5%였다. 그리고 파종 시기에 따른 ha당 생초수량은 파종시기가 늦어짐에 따라 30,090 kg에서 16,018 kg으로 적었으며 건물수량에 있어서도 14,230 kg에서 5,521 kg으로 적었다(p<0.05). 따라서 본 시험의 결과를 종합하면 우리나라 답리작 논에서 총체보리의 ha당 생산성을 높이기 위해서 파종시기는 10월 20일 이전이고 ha당 파종량은 220 kg/ha로 파종하는 것이 바람직하다고 생각된다.

• 한국초지조사료학회지
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• 제41권3호
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• pp.168-175
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• 2021

The optimal determination of seeding rate is critical to minimizing uncertainties about the large variations observed in forage quality and productivity when alfalfa is cultivated under different geographical areas and growing conditions. The objective of this investigation was to provide information about the proper seeding rate according to harvest timing for alfalfa cultivation in the Northern regions of Korea. Alfalfa was sown in September 2018 at a seeding rate of 20, 30 or 40 kg/ha and harvested four times in 2019: May 3, July 2, September 11, and October 13. Regardless of seeding rate, alfalfa plant height was longest at the third harvest (113 cm) and the shortest in the last annual harvest (43.8 cm). However, seeding rate had no effect on alfalfa plant height at any harvest. Forage relative feed value was increased in the first cutting but decreased in the third cuttings as seeding rate increased. However, seeding rate had slight effect on alfalfa forage quality components at the second and fourth cuttings. Total annual DM and crude protein production (in 4 harvests) was greater at higher seeding rates. Plots seeded at a rate of 40 kg/ha produced on average 1,257 and 2,620 kg/ha more forage (DM basis) than those seeded at a rate of 30 or 20 kg/ha, respectively. Forage DM production at the first, second, third, and fourth harvests accounted for 36.1, 24.0, 27.1, and 12.8 % of total annual DM production, respectively. Overall, small differences were seen when alfalfa seeding rate was different but maximum forage DM production (in four harvests) was detected when seeding rate was 40 kg/ha. These data could be useful to the alfalfa growers by allowing them to make more accurate trade-offs between seed price and the expected magnitude of forage yield gains in order to select the best seeding rate.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.707-714
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• 2004

How to improve the cell culture method on scaffolds is important in the tissue engineering fileld. In this study, we optimized seeding and culture methods to vascular smooth muscle cells (VSMCs) on biodegradable polymer scaffold. The primary culture of VSMCs obtained from canine external jugular vein was accomplished by applying the explant-derived method. The primary cultured VSMCs were seeded into scaffolds and then cultured by using various different methods; static or dynamic seeding, static or dynamic culture. The difference in proliferative response of VSMCs was analyzed with an alamar blue assay. Cell-polymer construct was examined by histochemical method and scanning electron microscopy. Mesh type scaffold ($10 \times 10 \times0.4 mm$) was made of polyglycolic acid (PGA) suture thread. The PGA mesh type scaffold was 45% in porosity, and 0.03 g in weight. The primary cultured VSMCs were confirmed with immunohistochemical staining using monoclonal anti-$\alpha$-smooth muscle actin. The density and distribution of proliferated VSMCs within the scaffold and cellular adherence on the surface of the scaffold showed better results in the static seeding condition than in the dynamic condition. Under the same condition of seeding method as the static condition, the dynamic culture condition showed enhanced proliferation rates of the VSMCs when compared to the static culture condition. In conclusion, to improve the VSMCs proliferation in vitro, static seeding is better than the dynamic condition. In the culture condition, however, culture under the dynamic status is better than the static condition. This was a pilot study to manufacture artificial vascular vessel by tissue engineering.

• 한국초지조사료학회지
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• 제29권3호
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• pp.171-178
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• 2009

본 연구는 청보리와 월동 콩과작물인 사료용 완두와의 혼파재배를 통해 사료작물로 이용하기에 적합한 작부체계로서의 혼파비율을 구명하고, 조단백질, ADF, NDF 등과 같은 사료가치와 수량을 만족하는 적정한 혼파비율을 비교 분석하기 위하여 실험을 수행하였다. 청보리와 사료용 완두를 혼파재배하였을 때 청보리의 생육특성에서 출수일과 초장은 큰 차이가 없었으며, 청보리의 경수는 사료용 완두의 혼파비율이 높을수록 감소하였지만, 사료용 완두의 개체수는 증가하였다. 청보리의 도복지수는 $0{\sim}3$의 분포로 나타냈는데, 그 중 사료용 완두의 혼파 없이 청보리 20kg/10a만을 파종하였을 때에 도복지수가 3으로 가장 높게 나타났다. 사료용 완두의 생육특성에서 월동률은 모든 파종구에서 90% 이상의 월동률을 보였고, 완두의 초장은 청보리의 파종량에 상관없이 사료용 완두의 파종비율이 높아질수록 증가하는 경향을 보였다. 청보리와 사료용 완두를 혼파재배하였을 때 생초수량은 청보리 14kg/10a 파종구와 20kg/10a 파종구 모두에서 10a당 3,000kg 이상의 수량을 나타냈으며 사료용 완두의 혼파비율이 높아질수록 증가하였으나 건물수량은 생초수량과는 반대로 완두의 혼파비율이 높을수록 감소하는 것으로 나타나 청보리 20kg/10a만을 단일 파종하였을 때 1,266 kg으로 가장 많은 건물수량을 나타냈다. 청보리와 사료용 완두의 혼파 재배했을 때, 조단백질, ADF 및 NDF 함량은 사료용 완두의 혼파비율이 높을수록 높아지는 경향을 보였다. RFV는 청보리만을 14 kg/10a 파종구에서 183.8로 가장 높았으며 사료용 완두의 혼파비율이 높을수록 작아지는 경향을 보였다. 표준화 점수의 크기로 본 가장 적합한 혼파조합은 청보러 20 kg/10a와 사료용 완두 7.5kg/10a 혼파 조합이 2.309로 가장 좋았고, 그 다음으로는 청보리 20kg/10a와 사료용 완두 5.0kg/10a 혼파구가 좋았으며 청보리 14 kg/10a 파종구에서는 청보리 14kg/10a와 사료용 완두 10.0 kg/10a를 혼파한 파종구가 0.354로 좋게 나타난 것으로 유추하여 보면 청보리 20 kg/10a에 사료용 완두를 7.5kg/10a를 파종하는 것이 생산성과 사료가치측면에서 합리적인 혼파비율로 판단되었다.

• 한국환경복원기술학회지
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• 제13권2호
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• pp.42-51
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• 2010

Herbs and shrubs are employed for environmental restoration purposes. Among common herbs and shrubs, few species with low germination rates were selected and studied for enhanced germination rates and decreased germination times via soaking treatment. Rhus chinensis, incubator grown samples treated with the bacterial solution for 72hrs followed by immediate seeding showed the highest germination rate of 26.7% and germination period of 5.7 days, 3 days decrease from the control. Treatment of distilled water (t=3.79, p<0.01), nutrient broth (t=4.44, p<0.00) and bacterial solution (t=4.42, p<0.00) showed highly significant difference. In the case of soil tests, treating in the nutrient broth for 72 hrs followed by immediate seeding yielded the the highest germination rate of 23.3% with 7.3 days to initial germination, a decrease of 14.7 days with respect to the control. All the samples followed by immediate seeding showed significant difference (t=2.13, p<0.05). Incubator grown samples of Lespedeza cyrtobotrya showed different results. The incubator samples suspended for 48 hrs in distilled water followed by immediate seeding and 1 day drying displayed the highest germination rate of 96.7%, surpassing that of the control by 33.4%. The incubator samples treated with the distilled water and nutrient broth showed enhanced germination. But only the samples treated with distilled water and nutrient broth for 48hrs showed the increased germination in soil tests. All the sample treated for 24 hrs followed by immediate seeding or dried for 1 day showed initial germination as early as 1 day in incubator. The initial germinations were shortened in the samples treated with distilled water and nutrient broth for 48hrs in soil tests. Lespedeza cuneata incubator sample treated with nutrient broth for 24 hrs and dried for 1 day exhibited the highest germination rate of 83.3%, a 31.1% improvement over the control. The incubator samples treated with distilled water for 48 hrs (t=4.20, p<0.01) showed effective increase of germination. The treatment of distilled water (t=2.96, p<0.05) and bacterial solution (t=2.24, p<0.05) showed significant difference. The germination rates in soil were less than those of incubator and the control. The incubator samples treated with distilled water and bacterial solution displayed 1 day germination period, shortened by 1.3 days compared to the control. For soil grown samples, the samples treated with distilled water showed delayed initial germination and those treated with nutrient broth for 48hrs and bacterial solution for 72hrs shortened initial germination.