• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.83.1-83
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• 2003

Rice seedling disease is one of major problems in water-seeded rice. This disease is known to be caused by several pathogen such as Pythium, Achlya, and Fusarium species. However, seedling disease of rice in water-seeded rice in Korea is not extensively studied. Pythium species have been isolated from Seosan, Yeoju, Icheon areas using Pythium selective media and their pathogenicity was investigated. All of the Pythium isolates showed strong pathogencity causing seedling emergence reduction in water-seeded rice. Seedling emergence was reduced to 0∼9％ at 10 days after inoculation of 23 Pythium isolates compared to 60％ of noninoculated control in a growth chamber. However, Fusarium species did not cause seedling emergence reduction in water-seeded rice. In contrast, when no water added into water agar or soil, the pathogen caused seedling rot two weeks after planting. These results indicate that Pythium species is a cause of seedling disease in water-seeded cultivation areas in Korea.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.305-312
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• 1995

Weed communities in the dry-direct seeded rice(Oryza sativa L.), soybean(Glycine max(L.) Merr.), and dry-direct seeded rice-soybean rotation system from the transplanted rice field were investigated in order to identify a change in weed species by paddy-upland rotation. Echinochloa species was the most dominant species in the dry-direct seeded rice and their great increase was observed in the continued practices of dry-direct seeded rice. Echinochloa spp., Chenopodium ficifolium, and Digitaria spp. were identified as the major weeds in soybean cultivation in paddy field. Practice of soybean rotation in the dry-seeded rice reduced weed occurrence, particularly at the soybean after 2-yr continued dry-direct seeded rice and at dry-direct seeded rice culture after 2-yr continued dry-direct seeded rice and at dry-direct seeded rice culture after 2-yr soybean compared to the continued cultures of rice or soybean. In addition, cultivation of dry-direct seeded rice or soybean culture in the paddy rice field seemed to be helpful for suppression of perennial weeds(Eleocharis kuroguwai, Cyperus serotinus, Scirpus juncoides) and aquatic weeds(Monochoria vaginalis, Sagittaria spp. Potamogeton distinctus).

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.278-288
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• 1995

Weed species occurred in dry- and water-seeded rice were 14 (2 grasses, 8 broadleaves, and 4 sedges) and 12 species (1 grasses, 7 broadleaves, and 4 sedges), respectively. Dominant weed species in dry-seeded rice were Lindernia procumbens, Cyperus difformis, Cardamine flexuosa, and Cyperus serotinus in discending order. The species in water-seeded rice were Cyperus difformis, Scirpus juncoides, and Lindernia procumbens. The number of weed species emerged in direct-seeded rice was determined at about 45 days after seeding, and dry weights of weeds were increased up to 75~90 days after seeding. Timing for great increase of plant height varied depending upon weed species. Regardless of weed number emerged, the most dominant weeds until late stage of growth were Cyperus difformis, Bidens frondosa, Digitaria ciliaris and Echinochloa crus-galli in dry- seeded rice, and Cyperus difformis, Echinochloa eras-galli and Scirpus juncoides in water-seeded rice.

• Korean Journal of Weed Science
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• v.18 no.1
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• pp.1-11
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• 1998

This experiment was conducted to investigate weed infestation and to determine effective weed control methods in direct-seeded rice. Twenty two weed species occurred in dry- and water-seeded rice, which was mainly composed of annual weeds. Dominant weed species in dry-seeded rice were Cyperus difformis, Echinochloa crus-galli, Aneilema keisak and Digitaria sanguinalis in discending order. Dominant weed species in water-seeded lice were E. crus-galli, C. difformis, Monochoria vaginalis and Scirpus juncoides. E. crus-galli emerged at 7 days after sowing. In water-seeded rice, E. crus-galli emerged at 5 days after sowing, and M vaginalis, S. juncoides and C. difformis at 8~10 days after sowing. Mean days to emerge important weeds was 20 days in dry-seeded rice and 13 days in water-seeded rice. Leaf development of weeds was faster than that of rice in dry-seeded rice. In water-seeded rice, E eras-galli was more vigorous than rice, but leaf development of other weeds were slower than that of rice. Changes in number of weeds and dry weight oil weed species varied depending upon weed species in the direct-seeded rice. Dry weight of weeds were increased greatly from 30 days to 60 days after sowing in dry-seeded rice. Number of weeds tended to increase up to 40 days after sowing drastically, and then trend of the increase was dull thereafter. Dry weight and number of weeds increased up to 20~60 days after sowing in water-seeded rice. Most effective herbicide treatments was foliar application of cyhalofop/bentazon at 20 days after sowing followed by fenoxaprop/bentazon at 45 days after sowing in dry-seeded rice. All herbicide treatments except foliar applications were very effective to control weeds in water-seeded rice. Slight phytotoxicity was observed in foliar applied fenoxaprop/bentazon at 45 days after sowing in water-seeded rice, but it did not affect rice yield.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.27-35
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• 2005

Eighty-five different cultivars of Brassica rapa, B. juncea, B. nap us, B. carinata, B. oleracea and hexaploid Brassica collected from Bangladesh, Japan, China and Denmark were analyzed by SDS-PAGE for seed and leaf protein variations, using esterase, acid phosphatase and peroxidase isozyme analysis. Ten polymorphic bands were identified from seed protein however no identifiable polymorphic band was found in the leaf protein. Polymorphic markers clearly distinguished the different Brassica species as well as yellow sarson (YS) and brown seeded (BS) cultivars of B. rapa. The $F_1$ cross between YS and brown seeded cultivars showed the existance of all poly-morphic bands of the respective parents. The Bangla-deshi and Japanese cultivars of B. rapa differed in the amount of seed protein. In the case of isozyme analysis, esterase showed the highest number of polymorphic bands (13) followed by acid phosphatase (9) and peroxidase (5). These polymorphic markers were very effec-tive for classification of all the species studied in this experiment. In parentage tests using isozymes, the hybridity of intra-and-interspecific crosses of almost all the seedlings could be identified from their respective cross combinations. Esterase polymorphism showed a clear differentiation between YS and BS types of B. rapa. In addition, two esterase polymorphic markers were iden ified to differentiate some cultivars of B. juncea. Segregation patterns in these two esterase bands showed a simple Mendelian monohybrid ratio of 3:1 in $F_2$, 1:1 in test cross and 1:0 in back cross progenies. No polymorphic band was identified to distinguish different cultivars of the same species by acid phosphatase or peroxidase. Polymerase Chain Reaction (PCR) was carried out with seed coat color specific marker of B. juncea. The yellow seeded cultivars produced a strong band at 0.5 kb and weak band 1.2 kb. In the addition of these two specific bands, Japanese yellow-seeded cultivars expressed two more weak bands at 1.0 kb and 1.1 kb. Where the brown seeded cultivars generated a single strong band at 1.1 kb. In segregating population, the yellow seed coat color marker segregated at a ratio 15 (brown) : 1 (yellow), indicating the digenic inheritance pattern of the trait.

• Korean Journal of Weed Science
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• v.13 no.2
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• pp.159-163
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• 1993

This research was conducted at the Crop Experiment Station to determine the changes of weed flora under direct seeded rice cultivation in dry paddy field from 1989 to 1992 and to establish an effective weed control strategy in direct seeded rice cultivation. With the continuous rice growing under direct seeded rice cultivation in dry paddy field, there were more occurrence in grasses and annual weeds while broadleaf and perennial weeds were diminished. The predominant weeds were aquatic weeds such as Eleocharis kuroguwai, Echinochloa crusgalli, and Monochoria vaginalis in machine transplanted rice paddy field while there were predominant of semi aquatic weed species such as E. crusgalli, Digitaria sanguinalis, Bidens tripartita, Aeschynomene indica, and Arthraxon hispidus in continuous direct seeded rice cultivation in dry paddy condition. Based on the vegetative analysis of weeds in terms of simpson index and community dominance, there was more distribution of weed species in direct seeded rice cultivation on dry paddy condition while only distributed with 1-2 weed species in machine transplanted rice paddy field. In similarity coefficient, there was a gradual increase up to 67% of weeds with the continuous direct seeded rice cultivation in dry paddy condition for 4 years starting from 41% of similarity coefficient.

• Korean Journal of Weed Science
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• v.12 no.3
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• pp.230-260
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• 1992

The paper was reviewed the research results on weed dynamics and effective control methods in direct-seeded rice crop. Direct seeding method resulted in drastic increment of weed growth compared to transplanting method and also changed in troublesome weed flora. Two to three fold more weeds were harvested at the direct seeded rice and weed flora of dominant species shifted toward $C_4$type grass weeds. Some of the important troublesome weeds in direct seeded rice were Echinochloa crus-galle, Oryza saliva ssp spontanea, Leptochloa chinensis. Setaria viridus. Digitaria adsendens, Sesbania exaltata, Aeschynomene indica, Algae, etc. Yield loss due to weed competiton was about 40-60% for water-seeded and about 70-100% for dry-seeded rice while these for transplanted rice were about 25-35% for mechanical transplanting and about 10-20% for manual transplanting, respectively. Integrated weed management concept was neede to approach weed control effectively. Several cultural technologies were very effective to suppress the weed growth. These were tillage operation, water management, seeding date and seeding rate. Crop residues of barley, rice, wheat, oat and italian ryegrass were also effectivly suppressed the paddy weeds particularly to Potamogeton distiuctus, a perennial broadleaf weed. A pathogen of Epicoccosorus nematosporus identified from Eleocharis kuroguwai was an excellent potential bioagent to control the most troublesome perennial sedge weed of E. Kuroguwai without arising any detrimental effect. The herbicidal efficacy of this pathogen was as high as bentazon herbicide. Plant growth regulator of paclobutrazol (pp-333) was another possible alternative to reduce the herbicide use. In current, herbicide exhibited the most conspicuous results to control weeds in direct-seeded rice even though the application technologies were not fully established. Recommendations for herbicide application were suggested for in both water-and dry-seeded rice in USA, Japan and Korea, respectively. To make better and comprehensive recommendations further studies on weed ecology and herbicide development were emphasized.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.53-61
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• 1999

This study was conducted to determine the optimal collecting time and methods of utilization of forest topsoil as a seed-bank source for revegetation materials of slopes. From April to November of 1998, nursery seedbeds in the greenhouse of Seoul National University were seeded with mixtures of forest topsoil and seeds. The nursery seedbeds were treated to evaluate the effect of depths of forest topsoil and seeding amounts. Seasonal experiments with $3{\times}3$ factorial design were conducted in April, June and August. More than 40 seedlings/$m^2$ were observed as the naturally-emerged species in the plot, treated with the top-layer forest soil within the depth of 10cm. However, it was found, as seeding more seeds, the number of appearance of the naturally-emerged species reduced because of the heavy competition between the seeded and the naturally-emerged. According to the results of this research, it is recommended that seeding amount should be no more than 1,000 seeds/$m^2$ to avoid the severe competition. Also it was observed that the forest topsoil collected in spring(April) is better than that collected in summer. The more species of the naturally-emerged were found in the forest topsoil collected in spring. Forest-topsoil-mixed-materials as a seed-bank source could significantly increase the plant diversity and productivity. Effective use of forest topsoil as a seed-bank source may become a valuable tool in future restoration of disturbed slopes for promoting plant community diversity and recycling of spoiled-soils from the slopes.

• Korean Journal of Weed Science
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• v.18 no.1
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• pp.12-19
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• 1998

The pattern of rice cultivation in Korea is changing from hand transplanting and machine transplanting into direct-seeding for low input and cost-down practices. However, there are still some obstacles to establish the direct-seeding cultural practices because of poor seedling stand, lodging, and weed infestation. In particular, there were much more and wide weed occurrence in direct-seeding rice as compared with the transplanted rice. Weed occurrence in terms of dry weight of weeds was highest in dry direct-seeded rice followed by sowing an puddled soil, sowing on submerged soil in order as compared with transplanting with infant rice seedling. Echinochloa crux-galli was a common dominant weed with 35~44% distribution in any direct-seeding methods. The dominant weed species were E. crux-galli, Cyperus difformis, C. serotinus, and Ludwigia prostrata in dry-seeded rice. The dominant weed species in water-seeded rice were E. crus-galli, Eleocharis kuroguwai, Scirpus juncoides, and Monochoria vaginalis. Yield loss of rice due to weeds at weedy condition was 96% in dry direct-seeded rice. In water-seeded rice, yield loss was 61%, whereas yield loss was 40% in machine transplanting with infant rice seedling.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.1
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• pp.141-154
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• 2012

This study was launched to verify the effective composition of plant species and its management program most suited for the dredged soiled area near Nakdong River Site 14. The improvement methods of planting base and the composition of plants such as silver grass, reed, and some colonies in aesthetic effect were studied. To search the management methods to decrease the confining pressure risen from the burried seeds which would consequently harm the previously seeded plants, experimental construction process was measured on the site. The purpose of this experiment was to figure out which part of the improvement on the plant base has the most significant effect for the revegetation of infertile, dredged soil, to verify the easily seeded, developing plants among seeded plants, and finally, to find the restoration model using plants near the dredged soil around riverside. 8 seeded plants and 23 invaded species were appeared which among the emerged plants, development of Aster yomena MAKINO, Lotus corniculatus var. japonica Regel, Trifolium repens L, and Dianthus longicalyx Miq were proved to be brought up well. Difference risen from the seed composition were not noticeable until 150day since the germination was proceeded mainly by Aster yomena MAKINO. The experimental plot with dredging sand+organic fertilizer method of construction and dredging sand+soil conditioner method showed most development while the effect of the plot with only the soil base of dredging sand stayed low. Another important method for the management of infertile, dredged soil base would be the removal of disturbing species which the experiment showed the tied relationship between the removal of disturbing species and development of seeding plants. Although this study was carried out focused on the Nakdong River Project, the study suggests the general management program that the removal of disturbing species such as Humulus japonicus Sieboid & Zucc. and Pueraria lobata (Willd.) Ohwi in times around rainy season(60days after seedling) would be effective for the easy growth of revegetation plants.