• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.4
• /
• pp.468-476
• /
• 2020

This study was performed to establish light intensity conditions for producing stem cuttings for aeroponic systems suitable for seed potato production using a closed-type plant production system. Shoot tip cultured plantlets of 'Sumi' and 'Chubaek' potato (Solanum tuberosum L.) were acclimatized, cuttings were collected, and stem cuttings were planted. The seedlings were raised for 40 days at different LED light intensities (60, 120, 180, and 240 μmol·m^-2·s^-1), and were cultivated in an aeroponic system for 80 days. When stem cuttings were raised at 60 μmol·m^-2·s^-1 LED light intensity, the plant height was the longest, at 17.3 cm for 'Sumi' and 16.1 cm for 'Chubaek', and the number of nodes was the highest in both cultivars. The higher light intensities, produced smaller plants with fewer nodes. The leaf areas, SPAD values, and Fv/Fm values differed slightly between cultivars. The fresh weight of stem cuttings, and the production rate of healthy stem cuttings were the highest at 60 μmol·m^-2·s^-1. In the aeroponic system, seedlings raised at 60 μmol·m^-2·s^-1 with LED light intensity showed a difference between the cultivars, but the fresh weight of stems and leaves above the planting plate was the heaviest. In addition, below the planting plate the stem cuttings were longest and the root weight was heaviest at 60 μmol·m^-2·s^-1 LED light intensity. The number of stolons also differed between cultivars, but was greatest for seedlings raised at 60 μmol·m^-2·s^-1 LED light intensity, at 4.2/plant for 'Sumi' and 7.7/plant for 'Chubaek'. At 60 μmol·m^-2·s^-1 LED light intensity, the tuber number and total tuber weight were the best, but the higher the light intensity, the smaller the total tuber number and total tuber weight for both cultivars. In conclusion, when producing potato stem cuttings for aeroponic systems using a closed-type plant production system, the most suitable LED light intensity for raising seedlings was found to be 60 μmol·m^-2·s^-1.

• Korean Journal of Plant Resources
• /
• v.37 no.2
• /
• pp.149-160
• /
• 2024

In Korea, potatoes have served as a side dish, but their role as snacks, such as chips or French fries, has recently gained traction. While there is a high demand for processing potatoes, there remains a dearth of research on the impact of double cropping, particularly during autumn, on processing quality. Therefore, this study was conducted to determine the effects of different spring potato cultivation periods on growth, yield, and processing quality during autumn cultivation. Following spring planting, harvest was carried out four times: 70 days, 80 days, 90 days, and 100 days. Subsequently, autumn cultivation was carried out in Gangneung and Seocheon regions using these seed potatoes. Results showed an increase in above-ground emergence rate with shorter spring growing period. When seed potatoes with a spring cultivation period of 80 and 90 days were grown in the autumn in the Gangneung region, the stem length was 47.2 to 48.9, which was greater than that of other treatments. However, stem number and leaf color (SPAD) showed no significant differences across cultivation periods. The number of tubers, tuber weight, yield, and marketable yield did not vary significantly with cultivation periods but described clear cultivar dependent differences. The tuber weight of the Saebong cultivar in Gangneung and the Eunseon cultivar in Seocheon was superior. The starch content peaked at 7.9% when seed potatoes grown for 80 days in the spring were harvested after autumn cultivation in the Gangneung region, but there was no significant differences in the Seocheon region. Glucose content showed a clear difference depending on the cultivation period, increasing with longer spring cultivation period during autumn cultivation. In conclusion, as a result of the effect of the spring potato cultivation period on the growth, yield, and processing quality of tubers when cultivating potatoes in double-cropping, the differences depending on the cultivation period were insignificant, while cultivar-based disparities were pronounced. The Eunseon cultivar exhibited robust above-ground growth and yield, while the Saebong cultivar demonstrated excellent processing quality.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.6
• /
• pp.447-451
• /
• 2004

This study was conducted to establish the proper techniques of the recirculating wick hydroponics for safe seed tuber proliferation of potatoes (Solanum tuberosum L. CV. Dejima). To achieve these, several intact tubers (5, 10 and 20 g) and cut seed-pieces (two or four) were treated in wick hydroponic system beds. A polystyrene box (31cm in width, 20cm in height, 51cm in length, and $0.031m^3$ in volume) was placed on a styrofoam hox. Eight wicks $(width\;1.5cm\;{\times}\;length\;40cm) $ were put into each polystyrene hox and the boxes were filled with perlite + peatmoss (1 : 2, v/v) medium. Top fresh weight per plant increased with increasing the tuber size from 10 to 30g/tuber. As the tuber size increases from 10 to 30 g/tuher in case of uncut tuber, the marketable tuber (>5g) production per plant increased from 83.8 to 141.8 g/plant and the marketable tuber (>5g) production per plant of cut tuber was slightly higher than that of uncut treatment. Total tuber yield ranged from 4.16 to $6.56kg/m^2$ and the percentage of seed tubers was greater than $97\%$ for all treatments. These results indicate that seed tuber should be cut to produce large tuber $(\geq10g)$ in the recirculating wick hydroponic system.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.55 no.1
• /
• pp.31-37
• /
• 2010

Two field experiments were carried out in 2002 to evaluate effects of the sizes of tubers produced through a wick-based hydroponics on growth and yield of seed potatoes (Solanum tuberosum L. 'Dejima'). Nine sizes of seed tubers (<1, 1~3, 3~5, 5~7, 7~10, 10~20, 20~30, 30~50, and 50~80 g) were used in spring crop. In fall crop, however, tubers except for the 1 g and 50~80 g wereused with tubers 7 and 15 g produced through aeroponics. Emergence rate greatly increased as tuber size was increased up to 10~20 g and then leveled off in both croppings. The number of days from planting to emergence in spring crop was about 34 days regardless of tuber size, while that in fall crop decreased from 34 to 16 days as tuber size increased from 1~3 to 30~50 g. The number of stems per plant, stem length and diameter, and leaf size tended to increase with tuber size. As the seed tuber size increased up to 10~20 g, the number of tubers per plant, average tuber weight, total and seed tuber yields linearly increased and then slowly increased with seed tuber size. Total and seed potato yields were not significantly different between tubers produced through a wick-based hydroponics and an aeroponics when similar sized tubers were planted in the fall cropping.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.54 no.3
• /
• pp.294-298
• /
• 2009

A glasshous experiment was carried out in 2002 to evaluate growth differences between two wick types, and to determine the optimum number of wicks in a wick-based hydroponics for production of 'Dejima' seed potatoes (Solanum tuberosum L.). The six minitubers ($7.0{\pm}0.2g$/tuber) produced through aeroponics were planted in a polystyrene box (51 cm long $\times$ 31 cm wide $\times$ 20 cm high) containing growth medium (perlite+peatmoss 1:2, v/v mixtures). Before the boxes were filled with the medium, 6 wicks per box were vertically and 2 to 10 wicks were horizontally installed, respectively, at the bottom of the boxes through holes. Comparing with 6 vertical wick treatment, 6 horizontal wick treatment increased the number of tubers per plant, but decreased average tuber weight. Yield of tubers greater than 5 g (seed potatoes) was not significantly affected by wick type. As the number of the horizontal wicks per box was increased from 2 to 6, the number of seed potatoes increased from 2.8 to 8.0/plant and then leveled off. Average tuber weight increased with increasing the number of the wicks up to 8 and then leveled off. As the number of the wicks per box was increased from 2 to 6, seed potato yield increased from 1.81 to 6.59 kg/$m^2$ and then slowly increased up to 10 wicks per box, reaching 12.9 kg/$m^2$. The results indicate that installing 8 wicks per box ($0.16m^2$) horizontally for the wick culture system in production of 'Dejima' seed potatoes is beneficial considering seed potato yield, cost of wicks, and convenient filling with medium.

• Korean Journal of Plant Resources
• /
• v.30 no.3
• /
• pp.298-310
• /
• 2017

Morphological variation between cultivated and weedy types of Perilla frutescens var. frutescens and P. frutescens var. crispa were studied in 327 germplasm by examining 17 morphological characters. The germplasm between the two varieties were varied for their qualitative and quantitative characters. The seed coat color of cultivated P. frutescens var. frutescens is commonly light brown and brown while deep brown color was observed in the weedy type P. frutescens var. frutescens and P. frutescens var. crispa. The leaf size, cluster length, plant height, flower number per cluster and seed weight in cultivated P. frutescens var. frutescens were significantly (P<0.05) different from weedy type P. frutescens var. frutescens and P. frutescens var. crispa. The cultivated P. frutescens var. frutescens exhibited significantly higher plant height (158.6 cm) compared to the weedy P. frutescens var. crispa (133.8 cm). Likewise, seed weight was significantly higher in cultivated (1.9 g) than in the weedy type of P. frutescens var. frutescens (1.6 g) and P. frutescens var. crispa (1.4 g). Principal component analysis (PCA) result showed that the first and second principal component cumulatively explained 86.6% of the total variation. The cultivated type P. frutescens var. frutescens and its weedy accessions were not clearly separated with P. frutescens var. crispa by PCA. Hence it requires the use of molecular markers for better understanding of their genetic diversity.

• Research in Plant Disease
• /
• v.20 no.1
• /
• pp.25-30
• /
• 2014

Phytophthora infestans was isolated from potato leaves collected from main potato producing areas in Korea during 2009-2011. In 2009, 99 isolates tested were all A1 mating type. Two of 64 isolates in 2010 and two of 78 isolates in 2011 were A2 mating type and they were found only in Miryang area. Among 99 isolates examined in 2009, 13.1% was resistant to metalaxyl, 3.1% was intermediate resistant and 83.8% was sensitive. In 2010, 19.4% of 62 isolates was resistant, 4.8% was intermediate and 75.8% was sensitive. Metalaxyl resistant, intermediate and sensitive isolates collected in 2011 were 23.1%, 9.0% and 67.9%, respectively. Metalaxyl resistant isolates increased mainly in winter cropping areas and seed potato producing areas where fungicides were sprayed more often. Frequencies of isolates showing minimum inhibition concentration of dimethomorph at $1.0-5.0{\mu}g/ml$ were 17.2% in 2009, 19.0% in 2010 and 15.4% in 2011. However, there was no evidence for occurrence of resistant isolate to dimethomorph because no isolate was able to grow at $5.0{\mu}g/ml$.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.2
• /
• pp.71-75
• /
• 1997

For the establishment of early selection of heat-tolerant clones through in vitro tuberization of true potato seeds, different temperature treatments for in vitro tuberization were investigated. The ratios of tuberization at 2$0^{\circ}C$ on var. Superior and DTO-33 treated with 5 mg/L of BAP and 500 mg/L of CCC, were 85% and 92%, respectively. At 3$0^{\circ}C$, the ratio of tuberization on DTO-33 was 37%, which revealed strongly heat-tolerant clone. In culture system of in vitro tuberization, the number of tubers per flask at 2$0^{\circ}C$ on non-subculture incubation was more than that on subculture incubation. The condition of non-subculture and short-day treatment for 4 weeks was good for production as 10.6 tubers per flask, which was very similar to that of long-day treatment. On the other hand, tuber diameter on long-day treatment was greater as 11.2 mm than on short-day treatment. Therefore, in vitro tuberization from true potato seeds could be induced under the condition of long-day treatment at darkness.

• Korean Journal of Environmental Agriculture
• /
• v.20 no.2
• /
• pp.116-121
• /
• 2001

To observe the stimulating effects of low dose ${\gamma}$ - radiation on the dormancy breaking and physiological activity, potato (Solanum tuberosum L. cv. Dejima) were irradiated at the dose of $0.5{\sim}30$ Gy. Low dose ${\gamma}$ - radiation exhibited promoting effects on the sprouting rate within the range of optimum dose of 1 Gy and 2 Gy and on the sprout length within the range of optimum dose of 2 Gy and 4 Gy. Regardless of storage duration and irradiation doses, the number of sprouts was enhanced. The number of sprouts, which were reserved for 15 days after production (DAP), were significantly increased under 4, 8 and 16 Gy irradiation. The growth of 45 DAP sprouts was extremely stimulated under 4 Gy irradiation accompanying the increase of peroxidase activity in the plantlet. In this study, it was also suggested that the activities of antioxidative enzymes of potato plantlets were not related to the irradiation dose during the plantlet development with the exception of decrease in catalase activity.

• Journal of Plant Biotechnology
• /
• v.1 no.1
• /
• pp.20-30
• /
• 1999

In order to modify lipid production of Perilla qualitatively as well as quantitatively by genetic engineering, genes involved in carbon metabolism were isolated and characterized. These include acyl-ACP thioesterases from Perilla frutescens and Iris sp., four different $\beta$-ketoacyl- ACP synthases from Perilla frutescens, and two $\Delta$15 a-cyl-ACP desaturases(Pffad7, pffad3). Δ15 acyl-ACP desa turase (Δ15-DES) is responsible for the conversion of linoleic acid (18:2) to $\alpha$-linolenic acid (ALA, 18:3). pffad 3 encodes Δ15 acyl-desaturase which is localized in ER membrane. On the other hand, Pffad7 encodes a 50 kD plastid protein (438 residues), which showed highest sequence similarity to Sesamum indicum fad7 protein. Northern blot analysis revealed that the Pffad7 is highly expressed in leaves but not in roots and seeds. And Pffad3 is expressed throughout the seed developmental stage except very early and fully mature stage. We constructed Pffad7 gene under 355 promoter and Pffad3 gene under seed specific vicillin promoter. Using Pffad7 construct, Perilla, an oil seed crop in Korea, was transformed by Agrobacterium leaf disc method. $\alpha$-linolenic acid contents increased in leaves but decreased in seeds of transgenic Perilla. Currently, we are transforming Perilla with Pffad3 construct to change Perilla seed oil composition. We isolated three ADP-glucose pyrophosphorylase (AGP) genes from Perilla immature seed specific cDNA library. Nucleotide sequence analysis showed that two of three AGP (Psagpl, Psagp2) genes encode AGP small subunit polypeptides and the remaining (Plagp) encodes an AGP large subunit. PSAGPs, AGP small subunit peptide, form active heterotetramers with potato AGP large subunit in E. coli expressing plant AGP genes.