Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Mating Types of Phytophthora infestans Isolates and Their Responses to Metalaxyl and Dimethomorph in Korea

감자 역병균(Phytophthora infestans)의 교배형과 metalaxyl 및 dimethomorph에 대한 반응

  • Kim, Jeom-Soon (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Young-Gyu (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kwon, Min (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Ju-Il (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Jee, Samnyu (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Kyeong-Hun (Ginseng Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 김점순 (국립식량과학원 고령지농업연구센터) ;
  • 이영규 (국립식량과학원 고령지농업연구센터) ;
  • 권민 (국립식량과학원 고령지농업연구센터) ;
  • 김주일 (국립식량과학원 고령지농업연구센터) ;
  • 지삼녀 (국립식량과학원 고령지농업연구센터) ;
  • 박경훈 (국립원예특작과학원 인삼과)
  • Received : 2013.11.05
  • Accepted : 2014.03.10
  • Published : 2014.03.31
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Abstract

Phytophthora infestans was isolated from potato leaves collected from main potato producing areas in Korea during 2009-2011. In 2009, 99 isolates tested were all A1 mating type. Two of 64 isolates in 2010 and two of 78 isolates in 2011 were A2 mating type and they were found only in Miryang area. Among 99 isolates examined in 2009, 13.1% was resistant to metalaxyl, 3.1% was intermediate resistant and 83.8% was sensitive. In 2010, 19.4% of 62 isolates was resistant, 4.8% was intermediate and 75.8% was sensitive. Metalaxyl resistant, intermediate and sensitive isolates collected in 2011 were 23.1%, 9.0% and 67.9%, respectively. Metalaxyl resistant isolates increased mainly in winter cropping areas and seed potato producing areas where fungicides were sprayed more often. Frequencies of isolates showing minimum inhibition concentration of dimethomorph at $1.0-5.0{\mu}g/ml$ were 17.2% in 2009, 19.0% in 2010 and 15.4% in 2011. However, there was no evidence for occurrence of resistant isolate to dimethomorph because no isolate was able to grow at $5.0{\mu}g/ml$.

2009년부터 2011년까지 주요 감자 재배지에서 채집한 잎으로부터 감자 역병균을 분리하여 교배형, metalaxyl과 dimethomorph에 대한 반응을 조사하였다. 2009년에 분리한 99개 균주는 모두 A1 교배형이었다. 2010년의 64개 균주 중 2개 균주와 2011년의 78개 균주 중 2개 균주만 A2 교배형이었으며, 이들은 밀양 지역에서만 분리되었다. Metalaxyl 저항성 조사 결과 2009년에 조사된 균주 중 13.1%가 저항성, 3.1%가 중도 저항성, 83.8%가 감수성이었으며, 2010년 균주 중에서는 19.4%가 저항성, 4.8%가 중도 저항성, 75.8%가 감수성으로 나타났고 2011년에는 저항성이 23.1%, 중도 저항성이 9.0%, 감수성이 67.9%로 나타났다. Metalaxyl에 대한 저항성은 약제 살포횟수가 많은 시설하우스 재배지와 씨감자 재배지에서 주로 증가하는 것으로 나타났다. 2009년에 분리된 균주 중 dimethomorph에 대한 MIC 값이 $1.0-5.0{\mu}g/ml$인 균주는 17.2%였고 2010년과 2011년에는 각각 19.0%와 15.4%로 나타났다. 그러나 $5.0{\mu}g/ml$에서 자라는 균주는 없어 dimethomorph에 저항성인 균주는 출현하지 않은 것으로 판단하였다.

Keywords

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