• Korean Journal of Environment and Ecology
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• v.34 no.4
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• pp.345-354
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• 2020

The purpose of this study was to investigate the effects of soil amendment treatments, such as hydroball, and active carbon, and planting Pennisetum alopecuroides for reducing calcium chloride (CaCl₂) of soil leachate and the growth of Pennisetum alopecuroides. The experiment planted Pennisetum alopecuroides in a plastic pot with a diameter of 10 cm and a height of 9 cm in a greenhouse April-October 2018. The experimental group comprised six treatments, including Non-treatment (Cont.), Hydroball (H), Active carbon (AC), planting Pennisetum alopecuroides (P), hydroball + planting Pennisetum alopecuroides (H + P), and active carbon + planting Pennisetum alopecuroides (AC + P). The dissolution of the CaCl₂ concentration 200ml of 10g/L was irrigated once every two weeks. We measured the growth (plant height, leaf length, leaf width, number of leaves), EC, pH, and exchangeable cations (K⁺, Ca²⁺, Na⁺, and Mg²⁺) according to the high concentration of CaCl₂ in the plant and soil leachate. In a treatment with the 'hydroball' amendment, the soil leachate electrical conductivity (EC), and the cation exchangeable were decreased more than those of the control, while the growth of Pennisetum alopecuroides relative growth rate(RGR) increased. Overall, application with the hydroball amendment added the planting of Pennisetum alopecuroides improved the salt reduction effect more than the control group. These results indicate that the application of the soil amendment agent hydroball was suitable soil amendments in accordance with the high concentration of calcium chloride (CaCl₂). Also, Planting Pennisetum alopecuroides is expected to be appropriate for salt-tolerant plant for soil affected by deicing salt agents.

• Food Science of Animal Resources
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• v.23 no.1
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• pp.9-15
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• 2003

All diets were based on feeds of fattening period pigs(LY x D, ca. 90 kg) with six treatments, which were the control, containing 5% beef tallow(C), 3% beef tallows and 2% perilla seeds oil(T1), 250 ppm vit. E(${\alpha}$-tocopheryl acetate) in T1(T2), 3% beef tallow and 2% squid viscera oil(T3), 250 ppm vit. E in T3(T4), and 3% beef tallow and 2% CLA(T5), respectively. Produced porks and their carcass characteristics were as follows. The daily gain of pigs was higher in T2 and 73 than any other treatments(p<0.05). Its T2 and T3 was 3.71 and 3.80 respectively, however, there was no significance in feed intake. The highest back fat thickness was shown in control group on market weight, while there was no significant difference on their initial weight. Loin-eye muscle area did not show any significant difference on initial weight and on market weight, however, its T5 was about twice as large as T2's. Content of triglyceride in blood was high in control group as compared to others; especially, the values for T3, T4 and T5 were significantly low(p <0.05). There was no significant difference in total cholesterol contents, and the ratio of HDL cholesterol/total cholesterol was higher in vit. E treated samples than untreated sample. Atherogenic index was high in sample with T3 and low in sample with T2. The perilla seed oil, squid fish oil, and vit. E decreased atherogenic index. Dressing percentage, back fat thickness, and grade did not show any significant difference(p >0.05); however, T2, C and T3, T1 and T5 showed 4.67, 4.29, 4.00 respectively, in grades.

• Research in Plant Disease
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• v.14 no.3
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• pp.193-200
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• 2008

Blending of eggshell powder into soil as ratio of 1:5, 1:10, 1:15, 1:20, and 1:25 did not affect seed germination rates of several crops including Chinese cabbage. The blending increased pH of distilled water and decreased the viability of resting spores of Plasmodiophora hrassicae. The ratio of non-viable resting spores in eggshell-blending water was over five times higher than in distilled water of the same pH. Chinese cabbage (cv. 'Norangbom') grew more in eggshell-blended soil than in non-treated soil, but other crops grew less. Leaf numbers and above ground growth of Norangbom increased to around 150% and 470%, respectively, in soil blended with $1:20{\sim}1:15$ of eggshell powder. Even though the optimum sizes of eggshell powder were $0.8{\sim}2.0mm$ for growth and smaller than 0.4 mm for inhibition of clubroot disease of Chinese cabbage, there was no statistical difference among the sizes. Soil pH was above 8.0 in all eggshell treatments without any statistical difference among them. Eggshell powder blending to 1:20 showed lower control efficacy, 58.5%, than registered fungicide 'Hokanna (flusulfamide)', 78.5%. However, Chinese cabbage of that blending ratio recorded the highest growth among the treatments. Therefore, blending of eggshell powder into clubroot-contaminated soil may make culture of Chinese cabbage possible by growth-increasing, even though eggshell powder could not inhibit clubroot disease entirely.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.61-62
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• 2003

Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022$^{\circ}$C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of either germination medium ($\frac{2}{1}$strength Murashige and Skoog (1962) salts with 2.5 g/l activated charcoal) or same medium with copper sulphate adjusted to 0.25 mg/1 to compensate for copper adsorption by activated carbon. 2% and 4% maltose was substituted by 7.5% and 13% PEG respectively to improve the yield of the embryos. Substitution of' maltose with PEG was clearly beneficial to embryo development. When 2% of the maltose was replaced with 7.5% PEG, many embryos developed to large bullet-shaped embryos. At latter stages of development most embryos callused and stopped development. A few short, barrel-shaped cotyledonary embryos formed that were covered by callus on the sides and base. When 4% of the maltose was removed and substituted with 13% PEG, the embryos developed further, emerging from the callus and increasing yield slightly. Microscopic examination of the cultures showed differing morphologies, varying from mostly single cells or clumps to well-formed somatic embryos that resembled early zygotic embryos only liquid cultures with organized early-stag. A procedure for converting and acclimating germinants to growth in soil and greenhouse conditions is also tested. Seedling conversion and growth were highly related to the quality of the germinant at the time of planting. Germinants with larger shoots, longer, straighter hypocotyls and longer roots performed best. When mature zygotic embryos germinate the root emerges, before or coincident with the shoot. In contrast, somatic embryos germinate in reverse sequence, with the cotyledons greening first, then shoot emergence and then, much later, if at all, the appearance of the root. Somatic seedlings, produced from the maturation medium, showed 100% survival when planted in a field setting. Somatic seedlings showed normal yearly growth relative to standard seedlings from natural seed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.1
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• pp.19-27
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• 2017

The objective of this study was to determine the growth characteristics of cool (C1) and warm season grasses (C2) in pastures mixed with C1 and C2 at an altitude of 400 m in Jeju island to establishing pasture suitable for grazing horses and to evaluate the effect of pastures mixed with tall and short type grasses on the intake characteristics of horses. C1 used in this study was Kentucky bluegrass, redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. Treatments of this study were consisted of four groups and the short type grass used in pastures mixed with C1 and C2 was mainly bermudagrass. Four treatment groups were follow as; Treatment 1 (bermudagrass + Kentucky bluegrass + redtop) 2) Treatment 2 (bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + redtop) 4) Treatment 4 (tall fescue + orchardgrass). Bermudagrass was a little winter killing and inhibition of plant growth at an altitude of 400 m. Plant heights in pastures mixed with C1 and C2 were grown better than that in pastures mixed with C1. Especially, plant height in Treatment 4 was higher than other treatments. Dry matter yield was in the following order: Treatment 4> Treatment 3> Treatment 2> Treatment 1. Dry matter yield in pastures mixed with C1 increased as compared with pastures mixed with C1 and C2. Dry matter yield in Treatment 3 was higher than other treatments. In the first investigation regarding vegetation distribution, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 11.7 and 13.3%, respectively. The growth of bermudagrass in winter was low due to the cold damage. However the growth of Kentucky bluegrass, redtop, tall fescue and orchardgrass was good. In the second investigation, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 5.0 and 11.7%, respectively. Growth of forage in the second investigation was poor as compared to the first investigation. nutritive values(crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility) were good in pastures mixed with C1 Especially, nutritive values in pastures mixed with tall was higher than those of pastures mixed short grasses. P content among minerals in Treatment 1 was higher than other groups. However, the content of Ca, Mg and Mn were lower. The contents of Ca, K, Mg, Na, Cu, Zn and Fe in Treatment 2 were higher. However, the contents of K, Mg, Na, Cu, Zn and Fe in Treatment 3 were lower. Therefore, we suggest that cool season grasses with short grasses were sowed to establishing pasture suitable for grazing horses at an altitude of 400 m in Jeju island.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.2
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• pp.165-173
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• 1985

The objectives of the study were to investigate the effects of foliage clipping on photosynthesis and grain filling for branch and non branch types under the polyethylene film mulch and non mulch conditions in mono cropping and second cropping after barley in sesame (Sesamum indicum L.), and to improve poor grain filling at later flowering time utilizing these data. One thousand grain weight was more decreased in branch type than in non branch type, in polyethylene film mulch condition than in non mulch condition, and in second cropping after barley than in mono cropping by clipping lower part foliage. Twentyfive percent clipping of lower part foliage showed a little increase than no clipping. Matured grain rate also showed same tendency between branch and non branch type and between mono cropping and second cropping after barley as well as 1,000 grain weight except for polyethylene film mulch. Matured grain rate of 25% foliage clipping at 30 days after flowering in non branch type presented a little increase but decreased in branch type. Clipping of higher part leaves were so serious decrease of matured grain rate that higher part leaves at late maturing time have a major role in photosynthesis. Matured grain rate of foliage clipping at 10 days after flowering was decreased in all treatments. Chlorophyll content of higher part leaves at 50% lower part foliage clipping presented 39% increase compared to same positioned leaves of non treatment, and 66% increase by 50% higher part foliage clipping in lower part leaves. Photosynthetic activity was 58% more increased in 50% lower part foliage clipping than no clipping, but seriously decreased in 50% higher part foliage clipping. Therfore, photosynthates of remained lower part leaves could not only support their own demands, but also any contribution to translocation of photosynthates from source to sink at late maturing time. Harvest index was 28% increased in 25% lower part foliage clipping and 13% decreased in 50% higher part foliage clipping compared to no clipping. Leaf area was 48% increased in 50% lower part foliage clipping compared to the same positioned leaves of no clipping, and only 5% increased in higher part foliage clipping. Productivity by foliage clipping compared to non treatment, was highly decreased in branch type than in non branch type, in second cropping after barley than in mono cropping. Little difference was detected between polyethylene film mulch and non mulch conditions. Twenty five percentage of lower part foliage clipping on mono cropping of non branch type appeared 5% and 8% yield increase in each of polyethylene film mulch and non mulch conditions compared to no clipping, and all decreased in other treatments. Mean loss of productivity by foliage clipping at 10 days after flowering was serious than clipping at 30 days after flowering. As the result, contribution to photosynthesis of source at 10 days after flowering are larger than that at 30 days after flowering in sesame. Fifty percent lower part foliage clipping at 10 days after flowering showed so the most serious yield decrease that lower part leaves at that time were considered as the main role leaves for photosynthesis.

• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.611-620
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• 2007

The current study was designed to define whether a blend of prunus mume extract(25%) containing lactic acid(75%) and grape seed extract(10ppm) could affect in vitro antimicrobial activity and growth performance, intestinal microflora, plasma biochemical profiles and digestive enzymes activities in broiler chickens. In paper disc agar diffusion test, we clearly observed antimicrobial activity against E. coli in response to prunus mume extract or a blend of prunus mume extract. For in vivo test, a total of ninety six 3-d-old male broiler chicks were assigned to basal diet(CON), basal diet supplemented with antibiotics (ANTI) and 0.5% a blend of prunus mume extract(PRNUS) until 35 days of age. Throughout the entire experimental period(3-35 days), there were no differences in BW and FCR between the birds fed the basal diet with antibiotics and the diet supplemented with a blend of prunus mume. However, ANTI group showed a significant increase in BW and total gain compared to CON group. The weights of digestive organs such as the pancreas and mucosal tissues were not affected by dietary treatments. There was no difference in plasma levels of glucose, cholesterol, AST and ALT activity. However, triglyceride in plasma increased(P<0.05) in the birds fed the diet supplemented with 0.5% a blend of prunus mume extract compared to those fed antibiotics supplemented diet. The activities of pancreatic trypsin and amylase, and intestinal hydrolase including disaccharidase were not affected by dietary treatment. The colony forming units(CFU) of lactobacillus in the lower ileal-cecum of the birds fed the diet supplemented with a blend of prunus mume extract was significantly(P<0.05) higher than that of birds fed antibiotic supplemented diet without affecting the CFU of E. coli. In conclusion, the birds fed the diet supplemented a blend of prunus mume as an alternative to antibiotics showed a similar growth performance and an significant increase in lactobacillus population compared with the birds fed basal and antibiotics supplemented diets.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.2
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• pp.84-90
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• 2018

Cultivation of high-quality forage crop by using fallow field during winter is required for national high feed self-sufficiency and establishment of self-supply system of high-quality forage crop. Field experiments for cultivation of high-quality winter forage crop were conducted at the paddy and upland fields in Cheonan and Anseong city with treatments of single Italian ryegrass(IRG) and IRG mixed with forage barley at the paddy field and of single forage barley and forage barley mixed with IRG at the upland field in the Mid-west plain. Several cultivation conditions such as broadcasting IRG seed under standing rice, sowing time, tillage method, drainage condition, mixed sowing with forage barley were compared to know the change of growth, yield and quality of winter forage crop. In particular, over-wintering rate and dry matter yield were decreased significantly in late-sown IRG and moisture-stressed forage barley. Yield and quality of forage crops were increased by sowing after tillage, mixed sowing of IRG with barley at the paddy field with good drainage. High yield as much as dry matter $10MT\;ha^{-1}$ with high feed value could be obtained by early sowing of feed barley mixed with IRG at the upland field. Cultivation conditions such as early sowing, sowing after tillage, drainage management are required for higher dry matter yield, quality and stable cultivation of winter forage crops in the mid-west plain of Korea.

• Journal of Korean Society of Forest Science
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• v.81 no.2
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• pp.156-163
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• 1992

Pinus koraiensis (Pk), P. rigida (Pr) and P. rigida ${\times}$ P. taeda (Pr. t) seedlings in a bare-rooted nursery were artificially inoculated with Pisolithus tinctorius (Pt) and Thelephora terrestris (Tt) to test long term effects of ectomycorrhizal inoculation on host growth. Mycelial inocula of Pt and Tt were mass-cultured in vermiculite-peatmoss mixture and introduced into fumigated nursery soil before seed sowing. Bare-rooted, inoculated seedlings at one to four years of age were outplanted to the field with $P_2O_5$ content of 25 ppm in soil. At the time of outplanting, Pk seedlings(4 years old), Pr seedlings(2 years old), and Pr.t seedlings(1 year old) all infected by Pt were significantly taller by 28%. 26%, and 77%, respectively, than controlled seedlings infected by natural population of mycorrhizal fungi in the non-fumigated plot. Ten years after inoculation or six to nine years after outplanting, Pk seedlings inoculated with Pt were significantly taller by 9% Pr.t seedlings significantly taller by 18%, and Pr slightly Caller by 2%(not significant) than controlled seedlings, suggesting that the stimulatory effect of Pt on host growth gradually declined or became minimal after outplanting. Tt failed to stimulate host growth either in the nursery or in the field, and the survival rate of outplanted seedlings was not different among fungal treatments. Considerable loss of the infected root system during lifting the seedlings for outplanting would be the primary cause of the reduced effect of Pt in the field. Pt infected more than 90% of the fine roots in the fumigated nursery during the first growing season, but Pt assumed to fail to compete successfully with natural population of ectomycorrhizal fungi in the field. It is necessary to select other mycorrhizal fungi which adapt well in both nursery and field.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.295-300
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• 2008

This experiment was conducted to compare forage production and feed value of winter forage crops at the experimental field of Livestock Division, National Institute of Animal Science from 2003 to 2004 in Jeju. The experiment was arranged in slit plot design with three replications. Main plots consisted of two cutting times, one time cutting and two times cutting. Sub plots consisted of 5 different seed combinations, T1 (Italian ryegrass), T2 (Oat), T3 (Italian ryegrass 25+Oat 75%), T4 (Italian ryegrass 50+Oat 50%), T5 (Italian ryegrass 75+Oat 25%). One time cutting showed the highest dry matter (DM) yield by 18,680 kg/ha. Tow times cutting showed the highest crude protein (CP) content by 11.97%. The all treatments have narrow range of total digestible nutrient (TDN) from 60.6% to 70.4%.