• Korean journal of applied entomology
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• 제12권3호
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• pp.115-120
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• 1973

An experiment was carried out to investigate possible use of several systemic insecticides as seed treatment to control some insect pests of rice, barley and soybean. The insecticides were impregnated with active carbon dust and $2\%$ water solution of methyl cellulose as skicker. The seeds were dressed with the insecticides: Fussol (Monofluoroacetamide), Dimethoate (0, 0-dimethyl-S-(N-methyl carbamoylmethyl) phosphorodithioate), Metasystox (0, 0-dimethyl 0-2 ethylthioethyl phosphorodithioate), Dimecron (0, 0-dimethyl (diethylamide-1-chlorocrotonyl) Phosphate). The seeds were treated at the rate of $4\%$ by grain weight. Effects of seed treatments with systemic insecticides on the seed germination and insecticidal effectiveness were tested. Fer rice, no seed germination was observed in Fussol treatment. Dimethoate, Metasystox and Dimecron showed a slight reduction in germination when seeds were sown 1 day after treatment, but they showed gradual reduction in germination when seeds were sown 20 and 45 days after treatment. Especially Diriethoate treatment showed significant reduction in germination after 20 and 45 days of storage. All the insecticides reduced seedling stands of barley somewhat. No plant emergence was observed in Fussol treatments after 25 and :to days of storage. In case of the 40 days of storge in barley seed treated with Dimethoate, Hetasystox and Dimecron there was no gignificant reduction in plant emergence except Fussol treatment. Dimethoate was highly toxic to the soybean seed viability. However, there was no significant reduction in the soybean germination after 1 day of storage. in Fussol, Metasystox and Dimecron treatments After 25 days of storag in Fussol and Dimethoate treatments, no plant emergence was observed, and there was $13.3\%$ emergence in Metasystox treatment, and $93.0\%$ emergence in Dimecron treatment. Dimethoate treatment in rice seed(variety Suwon # 82) showed $90\%$ mortality at the twentieth day and $33.3\%$ at the thirtieth day after sowing against the green rice leafhopper, Nephotettix cincticeps But no visible effectiveness was observed in Metasystox and Dimecron treatments. Dimethoate treatment in barley seed resulted in low population density of aphids. Rhopalosiphum spp. Final observation on the number of eggs per plant was made at the fiftieth day after sowing; 2 in Dimethoate, 4 in Fussol, 6 in Metasystox, 7 in Dimecron, and 13 in check plots. All the insecticides tested resulted in good insecticidal effectiveness against the aphids, Aulocorthum solani on soybean plant at the ninth day infestation after sowing. Dimethote, Metasystox, and Dimecron treated soybean plants was still effective in controlling the aphids to the eighteenth day infestation.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.225-234
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• 2011

Arabidopsis mutants with T-DNA insertion in seven calmodulin genes (CAM) were used to determine the specific role of CAM in the tolerance of plants to oxidative stress induced by paraquat and hydrogen peroxide ($H_2O_2$) treatments. Arabidopsis calmodulin mutants (cam) were screened for seedling growth, seed germination, induced oxidative damage, and levels of ${\gamma}$-aminobutyric acid (GABA) shunt metabolites. Only the cam5-4 and cam6-1 mutants exhibited an increased sensitivity to paraquat and $H_2O_2$ during seed germination and seedling growth. In response to treatments with $3{\mu}M$ paraquat and 1 mM $H_2O_2$, only the cam5-4, cam6-1 mutants showed significant changes in malonaldehyde (MDA) levels in root and shoot tissues, with highly increased levels of MDA. In terms of the GABA shunt metabolites, GABA was significantly elevated in root and shoot tissues in response to the paraquat treatments in comparison to alanine and glutamate, while the levels of all shunt metabolites increased in root tissue but not in the shoot tissue following the $H_2O_2$ treatments. GABA, alanine and glutamate levels were significantly increased in root and shoot of the cam1, cam4, cam5-4, and cam6-1 mutants in response to paraquat (0.5, 1 and $3{\mu}M$), while they were increased only in the root tissue of the cam1, cam4, cam5-4, and cam6-1 mutants in response to $H_2O_2$ (200 and $500{\mu}M$, 1 mM). These data show that the cam5-4 and cam6-1 mutants were sensitive to the induced oxidative stress treatments in terms of seed germination, seedling growth, and oxidative damage. The accumulation of GABA shunt metabolites as a consequence of the induced oxidative stress treatments (paraquat and $H_2O_2$ treatments) suggests that the GABA shunt pathway and the accumulation of GABA metabolites may contribute in antioxidant machinery associated with reactive oxygen species and in the acquisition of tolerance in response to induced oxidative stress in Arabidopsis seedlings.

• Korean Journal of Soil Science and Fertilizer
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• 제38권2호
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• pp.78-84
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• 2005

This study was conducted to investigate the effects of chemical treatments and electric and magnetic field treatments on germination and seedling growth of onion (Allium cepa L.). Germination ratios of the onion seed were not much different among the tested species. However, germination ratio was much higher in species of small and medium size seed than that of large one. Moisture content of the seeds was shown to be more suitable in the range of 10-15% for seed germination compare to that of 20%. The germination ratio of onion seeds was found to be increased by the chemical treatments in the order of GA<$NaNO_3$<$KNO_3$. In particular, the treatments with 0.1 and 0.2% $KNO_3$ increased the germination ratio by 13 and 15% compared to that of the non-treatment. The seeds treated with electric or magnetic field resulted in much higher germination ratio and better initial growth. Germination ratios of 86 and 88% were found in the 10 kV and 4 Gauss treatments, respectively. The length and weight of the seedlings grown for 40 days after transplanting were increased by 23-45% with 8 kV and 12 Gauss treatments compared to those of non-treatment.

• Journal of Ginseng Research
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• 제32권2호
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• pp.155-160
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• 2008

The North American ginseng (Panax quinquefolius L.) seed crop varies from year to year. The ability to hold stratified seed for a year would ensure continuity of seed supply and no interruption in production cycles. Seed drying and rehydration protocols at room temperature $(21{\pm}2^{\circ}C)$ were developed. These protocols and seed storage at 4 ${\pm}1^{\circ}C$ and 35%, or variable, relative humidity (RH) allowed the holding of stratified seed for one year and then establishment of the following five treatments in field plots: Trt.1 : dried 2005 stratified seed (seed harvested Fall 2004) held at $4^{\circ}C$ and at variable humidity; Trt.2 : 2006 stratified seed planted directly into the field; Trt.3 : 2005 stratified seed dried in October 2005 and held at $4^{\circ}C$ and 35% RH ; Trt.4 : 2005 stratified seed held in moist sand from October to December 2005 at room temperature $(21{\pm}2^{\circ}C)$ and then in December dried and held at $4^{\circ}C$ and 35 % RH; Trt.5 : 2005 stratified seed held in moist sand from October to December 2005 at room temperature and then in December dried and held at $-12^{\circ}C$ Seedling emergence was best in Trts. 2 and 4 with 67.3 and 65.1% respectively which is similar to the industry expected rate of 68% after regular stratification. Seedling growth was similar in Trts. 2 and 4 with root dry weights of 172 and 159 mg respectively in mid-August. Therefore, if holding stratified seed in August/September for one year is desired, the seed can be placed in moist sand until December and then dried and stored at $4^{\circ}C$ and 35% RH. These seed can be planted in the following August/September and will germinate and grow in the following year to give an acceptable crop.

• Journal of The Korean Society of Grassland and Forage Science
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• 제7권1호
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• pp.49-54
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• 1987

Information is needed on the plant characteristics affects by forage removal and their relationships to grain yield of italian ryegrass in order to combine herbage seed crop management and livestock farming. This study with tetraploid cv. Tetrone was carried out on the experimental field of Haenam Branch Station. treatments included two different seeding time, cutting time and frequency. Seeds were sown in row 50 cm width within the rows. The results are summarized as follows: 1. Fresh and dry matter were increased significantly in plots defoliated lately and many times but seed yield components were decreased significantly in plots defoliated in spring. 2. Autumn defoliation had no significant effect on the seed yield but seed yield were decreased significantly in plots defoliated later than 8 April. 3. Excess vegetative growth can be removed in autumn and early spring without harm to the seed crop and the most important aspect of its removal is the timing of the operation. 4. Seed yield reductions were accompanied by the decrease of inflorescences,seeds/spike, and thousand seeds weight, due to an increase in apex removal of vigorous tillers by late cutting.

• Journal of Practical Agriculture & Fisheries Research
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• 제24권1호
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• pp.29-35
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• 2022

This study was conducted to investigate the seed storage method and seed germination characteristics of Synurus deltoides (Ait.) Nakai, a kind of wild vegetables. Several parameters affecting seed germination, such as germination temperature, chilling duration, imbibition duration, and storage method were evaluated. The seed germination of Synurus deltoides showed the highest germination rate (GR), promptness index (PI), germination performance index (GPI), mean daily germination (MDG) at 25℃. This implies that the optimum germination temperature was 25℃. And also, Seeds stored at -4℃, after 150 days showed relatively high germination rate about 98%. Therefore, The optimum germination temperature of Synurus deltoides was 25℃ and to increase the germination rate over 98% of Synurus deltoides, cold-wet storage 20 days treatments at 4℃ were effective in the improvement of seed germination.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제34권4호
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• pp.370-377
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• 1989

Jerusalem artichoke (Helianthus tuberosus L.) as a biomass potential crop has several distinct advantages such as vigorous growth on poor land and high yield of carbohydrate. In this crop, seed dormancy has hampered the efforts of seed-propagration and to use them in breeding programs for improving jerusalem artichoke. Several seed treatments were tested to determine their effectiveness in overcoming the seed dormancy found in five collected varietes of jerusalem artichoke. The first results showed that the seed fertilities of five collected varieties ranged from 2.4％ to 14.7％ and the number of seed produced by one plant ranged from 88 to 1058. Germinability of seeds stored for 3 months at room temperature after harvest was almost 0％ and it was not improved by addition to the treatments of temperature, light and GA3, while germinability of seeds stored for 27 months at room temperature after harvest increased to 47.5％ in germination rate. But the removal and pin-pricking of seedcoat were very effective in breaking the seed dormancy, giving germination of 96.8％ and 82.3％, respectively. These results showed that the seed dormancy of jerusalem artichoke was induced by the seedcoat. Besides the treatment of seedcoat removal and seedcoat-pinpricking, the treatment of low and wet stratification was also effective in breaking the seed dormancy of jerusalem artichoke. Whole dormant seeds incubated for 70 days in low and wet condition germinated over 85％.

• Asian Journal of Turfgrass Science
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• 제18권1호
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• pp.29-36
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• 2004

The efficient refinement of seed is required to reduce the cost and labor input in artificial propagation of wild plant. This study was carried out to develop methods for collecting and refining tiny seeds from wild plants. For obtaining Phragmites communis seeds, the inflorescence was cut into small fragments using a Straw Cutter and subsequently detached pappus hairs from seed coat by Hammer Mill. The primary refined seeds were passed 1.0 mm sieve. The screened seeds were subjected to Seed Blower with wind speed of 0.25 mㆍsec-1 to collected intact and well-ripen seeds. The seeds of Imperata cylindrica were refined as follows. Inflorescences were cut using a Straw Cutter first. The pappus was removed from cut fragments using a Hammer Mill and subsequently subjected to Seed Scarifier at 500rpm for 60 sec. for further separation. The separated seeds were passed 1.0 mm screen and collected after blowing with Seed Blower of wind speed of 0.15 mㆍsec-1. When the amount of seed was too little to refine with Seed Scarifier and Blower, the procedure was slightly modified from the procedure described above. The crude seed mixture obtained from Hammer Mill step was hand-refined roughly and then immersed into cone. (95%) sulfuric acid for 2 min. and collected floating portion after dilution of sulfuric acid solution 100 times with tap water. The collected seeds were dried and passed 0.149 mm sieve. During seed refining process using mechanical or sulfuric acid treatments, a small portion of damaged seed were evolved, however, the amount was not noticeable as compared to the total amount of collected seeds. Because the germination percentages between hand-refined seeds and seeds refined by above methods were not statistically different, the developed procedures for refining tiny seed of wild plants are helpful to reduce the cost and labor input in artificial propagation of two species.

• Korean Journal of Medicinal Crop Science
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• 제10권2호
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• pp.75-81
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• 2002

Indoor seed treatments to elevate seedling emergence should be of value. The study was done to model the presown treatments of Platycodon grandiflorum seeds by evaluating the treatment effects of priming,$GA_3$, drying and water imbibition after drying on their germination and then their successive seed treatments on the basis of its seedling emergence. after priming using $Ca(NO_3)_2\;and\;GA_3$ treatment under their different concentrations and light quality illuminated for 12 hours a day were done separately and their two best results were compared to determine the better one, drying of imbibed seeds using the above best result and water imbibition of the dried seeds were successively done to check the rates of germination and emergence. In each treatment of priming and $GA_3$, the former best germination occurred at $Ca(NO_3)_2$ 150 mM done under 2 day darkness but the latter one did at 0.01 mM done under 12 hour a day red light forced for 3 days. Of the two best results from priming and $GA_3$ treatments, the latter result was shown higher germination rate. $GA_3$ treated seeds were best desiccated under $35^{\circ}C$ and 4 hour red light illumination. The germination rate of seeds dried after $GA_3$ treatment was enhanced as imibibed 2 days immediately before sowing. Seedling emergence of all 3 successive treatments, $GA_3$, drying and water imbibition before sowing was the greatest than the two others, only $GA_3$ treatment and the combination of $GA_3$ and drying, in which indicated that its presown seed treatment must follow the successive procedure of the above 3 ones.

• Journal of Bio-Environment Control
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• 제12권3호
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• pp.132-138
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• 2003

This experiment was conducted to investigate the mass propagation system of Aster glehni FR. and to obtain the basic data for improvement of germination and seedling. The following was the results of experimentation about temperature and light conditions, and priming treatment as kinds of chemicals, their concentration and treated periods affect germination of Aster glehni FR. The germination percentage of Aster glehni FR. seed was higher in 20 and 25$^{\circ}C$ than the others, but it dropped rapidly at 30$^{\circ}C$ and didn't germinate at 35$^{\circ}C$. The first day to germination was the slowest at 15$^{\circ}C$. The germination rate of Aster glehni FR seed increased with increasing with temperatures from 15 to 25$^{\circ}C$. But the seed was rotten easily in high temperature. The germination rate was shown highest in 25$^{\circ}C$, and next was 15, 20, 30$^{\circ}C$ in order. Light treatment enhanced germination percentage, the first day to germination, germination rate and T50, but there was no significant difference. The 3 hours priming treatments had more effect on germination percentage than 30 minutes treatments as comparing averages. Aster glehni FR seeds primed in $KNO_3+K_3PO_4$ for 3 hours had most eHective on germination percentage (83.3%) and also showed shortest $T_{50{\cdot}}T_{50}$ and day of first germination was better in 30 minutes than 3 hours treatments, and most of priming treatments were better than control(non-priming seeds). While priming seeds showed shorter day of first gemination than control, there was no significant difference between 30 minutes and 3 hours treatments.