• The Plant Pathology Journal
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• v.35 no.6
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• pp.684-691
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• 2019

Evolution of adaptive mechanisms to abiotic stress is essential for plant growth and development. Plants adapt to stress conditions by activating the abscisic acid (ABA) signaling pathway. It has been suggested that the ABA receptor, clade A protein phosphatase, SnRK2 type kinase, and SLAC1 anion channel are important components of the ABA signaling pathway. In this study, we report that the shaker type potassium (K⁺) channel, GORK, modulates plant responses to ABA and abiotic stresses. Our results indicate that the full length of PP2CA is needed to interact with the GORK C-terminal region. We identified a loss of function allele in gork that displayed ABA-hyposensitive phenotype. gork and pp2ca mutants showed opposite responses to ABA in seed germination and seedling growth. Additionally, gork mutant was tolerant to the NaCl and mannitol treatments, whereas pp2ca mutant was sensitive to the NaCl and mannitol treatments. Thus, our results indicate that GORK enhances the sensitivity to ABA and negatively regulates the mechanisms involved in high salinity and osmotic stresses via PP2CA-mediated signals.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.987-994
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• 2010

To investigate the effect of long term application of organic matter in upland soils, plots for treatments of NPK, NPK+pig manure compost, rape seed cake, rice straw compost, and green manure were set up. Populations of Bacillus and Gram negative bacteria were high in the plot treated with green manure application, but microbial biomass was increased with chemical fertilizer or pig manure compost in upland soils. Activities of phosphomonoesterase and dehydrogenase were high with organic matter application comparing to control. Cluster patterns analysed using phospholipid fatty acid of plots treated with rice straw and or pig manure compost were clearly different comparing with other treatments. Dominant bacteria in upland soils were Bacillus flexus, B. subtilis and B. megaterium. And the strains isolated from upland soils had amylase, protease and lipase activities.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.153-160
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• 1969

Experiments on sweet sorghum(sorgo) with different population per unit area were conducted from 1966 to 1968 for the first time in Korea. By increasing plant population stalk weight, refinable sugar and seed weight per plot were increased, but stalk weight per plant, brix percentage, sugar content, stem diameter and 1, 000 seed weight were decreased. Plant height, maturity and lodging were not affected by the treatments. The result obtained has suggested that the effects of plant spacing within row on the characteristics of plant growth and on yields were greater than those of row width. Negative correlations existed between sugar content and sugar yield, and seed weight per plant and seed yield. The optimal plant population in this study ranged from 16, 700 to 22, 200 plants per 10a, row width of 60cm and plant spacing in row of 15 to 20cm resulted in the highest sugar and seed.

• Journal of Ecology and Environment
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• v.43 no.2
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• pp.254-262
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• 2019

Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15-25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50-80% of the length of the seeds' development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L. insularis and L. harae germinated within 4 weeks after sowing at 15 ℃ and 20 ℃, respectively. Contrarily, L. praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 ℃, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 ℃, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 ℃). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 ℃) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 ℃, 20 ℃, 15 ℃, and 20 ℃, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.289-294
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• 2008

This experiment was conducted at college of Agriculture and Life Science in Chungnam National University from September, 2004 to June, 2007 in order to evaluate the mixture possibility between barley and rye in the area of Daejeon. Rye (cultivar; Koolgrazer) and barley (cultivar; Daeyeon Bori) were set for the experiment. The experiment was arranged in four treatments: R100 (rye 100%), R60 + B40(rye 60% + barley 40%), R50% + B50% (rye 50% + barley 50%), and R40% + B60% (rye 40% + barley 60%). The experiment was repeated three times in the randomized complete block. The average dry matter (DM) yield for three years of R100 weighed 9,282 kg and its DM yield was higher than any other DM yield. The higher the barley seed rates are, the lower the DM yield is (p<0.05). As the barley seed rates increased 40%, 50%, and 60% respectively, its vegetative percentage tended to increase 30%, 41%, and 47%, but the barley vegetative percentage against its seed rates did bring forth somewhat low results. Compared with R100, the contents of crude protein (CP) and in vitro dry matter digestibility (IVDMD) got higher as the barley seed rates became higher, while the contents of NDF, ADF, cellulose, and lignin were lower (p<0.05). Compared with R100, the yields of crude protein dry matter (CPDM) and digestible dry matter (DDM) showed lower in the any mixed barley (p<0.05). Thus, in case of using barley mixed with rye in the area of Daejeon, it seems to be quite difficult, unless the supply of high-productive barley variety is followed, to enhance the yields of DM, CPDM, and DDM.

• Journal of Animal Science and Technology
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• v.62 no.4
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• pp.495-503
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• 2020

The purpose of this study was to evaluate effects of replacing soybean meal with perilla seed meal (PSM) on growth performance, proximate composition of meat, meat quality, and fatty acids composition of meat in broilers. A total of 60 one-day-old chicks of ROSS 308 (initial body weight of 44.8 ± 0.4 g) were randomly allotted to four treatment groups (15 replicate cages with 1 broilers per cage) in a period of 5 weeks. Dietary treatments included control (CON) basal diet (corn-soybean meal-based diets) and test diets in which PSM was included at 0.5% (T1), 1% (T2), or 2% (T3) to gradually replace soybean meal. At the end of experiment, all broilers were slaughtered for collecting breast and thigh meat. At 5 weeks, T2 and T3 treatment groups had higher (p < 0.05) body weights than CON and T1 treatment groups. Weight gain was increased (p < 0.05) in T2 and T3 treatment groups. Feed intake was decreased (p < 0.05) in T3 treatment group. Broilers in T2 treatment group had lower (p < 0.05) feed conversion ratio than those in other treatment groups. In proximate composition of breast meat, there was no significant (p > 0.05) difference in moisture and ash. Fat composition of breast meat was significantly (p < 0.05) increased in T2 and T3 treatment groups. Regarding meat quality, broilers fed T3 diet had higher (p < 0.05) water holding capacity than those fed other diets. Regarding fatty acids composition of thigh meat, broilers fed T3 diet had higher (p < 0.05) linolenic acid than those fed other diets. In conclusion, soybean meal replaced by 2% perilla seed meal in broiler diet can improve growth performance, meat quality, and fatty acids composition of thigh meat in broilers. Perilla seed meal can be a lot of potential alternatives feedstuff for soybean meal in this study.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.186-186
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• 2017

Relay intercropping system of direct seeded rice and winter cereal is labor saving cultivation and has high land use efficiency in Japan. In this system, rice seeds are direct seeded into inter-row space of winter cereals (wheat or barley) in March or April. If the direct seeding of rice is conducted before stem elongation phase or using suitable seeder, these are little effect to yield of winter cereals. Though the seeds are generally thiuram treated, it's a matter that seedling establishment rate (SER) of direct seeded rice is low and unstable. The cause of low SER has not been revealed. In present study, with the aim to reveal causes of low SER, we conducted experiments and investigated the SER, and analyzed some factors that might affect SER. Experiment1: In 2015, 2016, we buried rice seeds underground, and investigated the transition of the seed survival rates (SSR). Seeds were thiuram-treated or non-treated. In 2 periods, SSR of thiuram-treated seeds were significantly higher than non-treated seeds. In 2016, thiuram-treated seeds were high in SSR (almost 75%) at April 30th, but low SER (10~27%) after harvest of winter cereals. Therefore, almost all of seed death might have been happen after germination. Analysis 1: We investigated the SER and cultivation conditions in Ibaraki pref. for several years. Meteorological factors were referred from the nearest point of AMeDAS. From mean temperature (MT) among 5days after and before the day, we divided the period of seeding ~June 20 to phase1~4. We defined each phase as below; Phase1: $MT{\leq}10^{\circ}C$, Phase2: $10^{\circ}C<MT{\leq}15^{\circ}C$, Phase3:$15^{\circ}C<MT{\leq}20^{\circ}C$, Phase4: $MT>20^{\circ}C$. We analyzed the correlation of SER and meteorological factors by each phase. Total number of days in phase 1~4 was significantly negative correlated with SER. In phase1, total rain fall and number of soil wetting days were significantly negative correlated with SER. In phase2~4, only MT was significantly positive correlated with SER. This result suggested that rainfalls in phase1 declined seed vigor to emergence from soil surface, by repeated water absorption and re-dry. From these present studies, it was suggested that one of factors of low SER of direct seeded rice in relay intercropping system is changing of water condition by rainfalls in phase1 ($MT{\leq}10^{\circ}C$). To improve SER, it's necessary to consider something seed treatments such as prevent water absorption during phase1. However, 58~60% of seeds seemed to die during May. It's suggested that, if seeds are thiuram treated, almost all of seeds can germinate underground, but the seed vigor to emergence from soil surface are insufficient. Further studies are needed to reveal the rest causes that is happening during emergence from soil surface.

• Korean Journal of Environmental Agriculture
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• v.11 no.3
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• pp.209-214
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• 1992

Residue of Propineb in Sesame(Sesamum indicum L.) seed was determined to establish an index for the safety use of Propineb to Sesame. Evaluation was made on residual concentration of Propineb in Sesame seed as a function of application frequency and date when the mixed formulations of Propineb(56%), protectant fungicide, and Oxadixyl(8%), contact fungicide, were sprayed into Sesame leaves. Level of Propineb treatment was $0.028g/m^2$ with various combinations of application time from three to sixty days before harvest. Results are summarized as followings. 1. Recovery percentages of Propineb from Sesame seed were ranged from 84 to 96, and the minimum detectable limit of Propineb with the method employed in this experiment was 0.03mg/kg. 2. Residues of Propineb in Sesame seed were in the ranges of 0.14 to 1.38mg/kg, varying with frequency and date of Propineb application. 3. Residues of Propineb increased as increasing application frequency of Propineb or as being application date closer to harvest time. 4. Residue of Propineb in Sesame seed was decreased with time, showing to be fitted to the first-order kinetics. 5. Residues of Propineb in Sesame seen were, irresepective of treatments, lower than 2mg/kg, the Maximum Residue Limit(MRL) established by FAO/WHO. 6. Half-life of Propineb determined in this experiment was ranged from 12 to 16 days.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.230-238
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• 2014

The purpose of this study was to develop the cost-effective and efficiency seed longevity prediction method of rice (Oryza sativa L.) germplasm for viability monitoring. To find an optimum predicting method for rice seed longevity at genebank, an accelerated ageing (AA) test, a controlled deterioration (CD) test and a dry-heat treatment (DHT) were conducted to the four groups of rice germplasm based on ecotype, such as Indica, Japonica, Javanica and Tongil type. Among the three artificial aging treatments, the dry-heat treatment of 36 hours at $90^{\circ}C$ is suggested as a routine predictive test method of rice germplasm longevity at a genebank. The distribution of germination rate on 3,066 accessions which conserved 26.5 years at $4^{\circ}C$ showed similar trend with the result of distribution by dry-heat treatment at $90^{\circ}C$ on 36 hours using 106 accessions of rice selected samples which composed four ecotype groups. The results show that the dry-heat treatment affect not only predicting the rice seed longevity but also determining effective interval for monitoring germination of rice germplasm in genebanks.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.43-48
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• 1997

This study was conducted to improve germination ratio of Zanthoxylum piperitum A.P. DC. seeds. Stratification for 60 days after scarification of seed with sand was germination percentage to 5.4% and $GA_3$, 50ppm for 24 hrs after scarification of seed with sand showed 8.9%. Soaking the seeds in $GA_3$, 50ppm for 24 hrs after 40 to $70^{\circ}C$ hot water treatment for 10 minutes showed low germination of 4.4%. Based on $H_2SO_4$, NaOH and $HNO_3$, treatments, germination percentage did not improve at all regardless of soaking time. The highest germination of 91.1% was observed when seed was soaked in $GA_3$ 100ppm for 48 hrs after stratification for 60 days at $4^{\circ}C$. Kinetin treatment at 50ppm for 24 hrs had the greatest germination percentage of 31.7% but it did not improve germination ratio compared to $GA_3$ treatment.