• Title/Summary/Keyword: Seed treatments

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Modified Drum Priming and Exogenous Application of 24-Epibrassinolide (24-EBL) for Enhancing Germination under High Temperature Condition in Lettuce Seeds

  • Kang, Won Sik;Kim, Min Geun;Kim, Du Hyun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.86-86
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    • 2018
  • This study was conducted to investigate the effects of modified drum priming and 24-Epibrassinolide (24-EBL) treatment to improve the seed quality for export. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 16 and 24 h in a container with a relative humidity of 99% at 26 rpm for a modified drum priming treatment. The treated seeds were sown at $20^{\circ}C$ and $30^{\circ}C$ (12/12h, light/dark) with four replications of 25 seeds on pleated paper. The seeds were hydrated with water or 24-EBL solutions of $10^{-7}$, $10^{-8}$ and $10^{-9}M$, respectively. The germination of the modified drum primed seeds (24 h incubation after 60% SMC hydration) improved to 1.6 days mean germination time (MGT) and $46%{\cdot}day^{-1}$ germination rate (GR), while the untreated seeds showed 2.1 days MGT and $28%{\cdot}day^{-1}$ GR. The modified drum priming (60% SMC and 24 h incubation with $10^{-9}M$ 24-EBL) showed improved results in MGT (1.8 days) and GR (55%) at $20^{\circ}C$, whereas untreated seeds showed 2.3 days MGT and 44% GR. Under $30^{\circ}C$, germination of modified drum primed seeds was significantly improved in GP (80%), GR ($31%{\cdot}day^{-1}$), HS (55%) and MGT (3.3 days), however, untreated seeds showed decreased GP (27%), GR ($22%{\cdot}day^{-1}$), HS (55%) and MGT (4.8 days). This study showed that the germination of lettuce seeds is enhanced by 24 h drum incubation with 24-EBL and this method can be used effectively to achieve the benefits of early germination and uniform seedling development. In addition, these treatments circumvent thermo-dormancy of lettuce seed and have a possibility of high-quality and environment-friendly seed processing.

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The effects of temperature, light and gibberellic acid on seed germination of Rhododendron weyrichii Max. (온도 , 광 및 Gibberellic Acid 처리가 참꽃나무의 종자발아에 미치는 영향)

  • 황환주
    • Korean Journal of Plant Resources
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    • v.8 no.1
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    • pp.41-46
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    • 1995
  • This experiment was carried out to investigate the effects of temperature, light quality and gibberellic acid on the seed germination of Rhododendron weyrichii Max. Optimum temperature for germination was $20^{\circ}C$ at constant temperature, but the final germination rate at $15^{\circ}C$ was about the same with the rates were attained by alternating temperature under higher temperature such as $25^{\circ}C$ or $30^{\circ}C$. The germination rate was increased by yellow and red light treatments at $20^{\circ}C$, but green light treatment strongly inhibited seed germination at $25^{\circ}C$. Gibberellic acid substitutes light requirement for germination of R. weyrichii seeds.

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Enhancing Yield and Nutritive Value of Forage for Livestock Feeding Through Corn Soybean Intercropping Strategy with Several Pre-sowing Soybean Seed Coatings

  • Kim, Jeongtae;Song, Yowook;Kim, Dong Woo;Fiaz, Muhammad;Kwon, Chan Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.1
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    • pp.50-55
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    • 2017
  • In attempt to avoid crop damage through wild bird's picking, this study was designed with aim to evaluate several pre-sowing soybean seed coatings for optimum yield in corn-soybean mixed forage. It was investigated under four cropping treatments, viz. 1) corn sole, 2) corn mixed with soybean without any coating, 3) corn with iron coated soybean and 4) corn with thiram coated soybean. Each treatment had three replicates and corn sole was control treatment. Pioneer (P1184) and crossbred ($PI483463{\times}Hutcheson$) seeds were used for corn and soybean, respectively. The trial was conducted under randomized block design from $5^{th}$ June to $23^{rd}$ September, 2015. Data were an alyzed through ANOVA technique using SAS9.1.3 software. Results depicted that survivability of soybean against wild birds damage was found better (p<0.05) in thiram coating which was higher than iron coating and control treatment but later on thiram coating had adverse effects on subsequent growth of soybean plants. Corn stalk height was decreased (p<0.05) in thiram coating, whereas corn ear height was reduced in iron coating treatment. Iron coating enhanced (p<0.05) height of soybean plant (p<0.05) better than that of thiram coating. Soybean seed coatings didn't influence dry matter yield and nutritive value in terms of total digestible nutrients yield in corn soybean mixed forage. Conclusively, although presowing thiram coating enhanced survivability of soybean plants against wild bird damage but had adverse effects on its subsequent growth. However, soybean seed coatings didn't influence yield and nutritive value of corn soybean intercropping forage.

Seed Treatment to Improve Germinability of Gourd (Lagenaria siceraria Standl.) (종자처리가 수박대목용 박 종자의 발아성에 미치는 영향)

  • Moon, Bo Shik;Jeong, Yeon Ok;Cho, Jeoung Lai
    • Horticultural Science & Technology
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    • v.17 no.6
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    • pp.747-749
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    • 1999
  • Various methods for seed treatments have been devised to improve the rate and uniformity of seed germination of gourd (Lagenaria siceraria Standl.). Hydropriming and priming, the imbibition of seeds in distilled water and osmotic solutions respectively, are useful techniques for early establishment of uniform seedlings. In this study, the optimum conditions for hydropriming and priming were determined in gourd (Lagenaria siceraria Standl.) seeds. Effect of hydropriming was compared with those of osmotic priming. Seeds, hydroprimed or primed with 50 mM solution of $KH_2PO_4$ and $KNO_3$ at $30^{\circ}C$ for 2 days, showed shorter $T_{50}$ (number of days to 50% of the final germination percentage) and MDG (mean number of days to germination) than those unprimed. The relative effectiveness of hydropriming and priming in improving the germinability of gourd seeds was more evident when germinated at $20^{\circ}C$ than at $25^{\circ}C$ and $35^{\circ}C$.

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The Effect of Deep Sea Water on Seed Priming of Sweet Pepper (Capsicum annum L.), Rice (Oryza sativa L.) and Ginseng (Panax ginseng C.A.Meyer)

  • Yoon Byeong-Sung;Shrestha Surendra Lal;Kang Won-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.411-417
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    • 2006
  • This experiment was conducted to study whether priming with deep sea water results in enhancement of seed germination and to identify the optimum concentration of the priming solution, and duration of priming using sweet pepper (Cv. California wonder), rice (Cv. Ilpum) and ginseng seed. Sweet pepper and rice seeds were primed with 5 various concentrations (5%, 10%, 15%, 20% and 30%) for deep sea water for 48 hours, 24 hours and 12 hours at $25^{\circ}C$ and ginseng seeds in 5%, 10%, 15%, 20%, 25% and 30%, and 2,4,6, and 8 electrical conductivity (EC) which were made by desalinating deep sea water. Priming in deep sea water (DSW) improved the early and final germination percentage, mean germinal on rate, emergence percentage and root and shoot length, compared with plain water, $KNO_3$ and without priming treatments. In sweet pepper, 24 hours priming with 5 percentage DSW significantly improved the early germination percentage and radical length. It has also improved the mean germination and emergence days and early emergence percentage, compared with $KNO_3$ and control. Whereas, in rice, 48 hours priming with 10 percent DSW significantly improved the early germination percentage, plumule emergence percentage, root length and shoot height. Hence the best seed priming treatment on sweet pepper and Rice are 24 hours with 5 percentage DSW and 48 hours with 10 percentage DSW, respectively, whereas in ginseng, priming with EC4, EC8 and 25% DSW had shown better germination.

Effects of Storage Duration with Low Temperature and Wet Condition, Germination Temperature and Shading Rate on Germination of Aruncus dioicus var. kamtschaticus Seeds (저온습윤 저장기간, 발아온도 및 차광율이 눈개승마 종자의 발아에 미치는 영향)

  • Song, Ki Seon;Jeon, Kwon Seok;Choi, Kyu Seong;Kim, Chang Hwan;Park, Yong Bae;Kim, Jong Jin
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.5
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    • pp.370-378
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    • 2015
  • Background : Aruncus dioicus var. kamtschaticus, functional wild vegetable, is perennial herb and young leaves with soft texture are generally used as edible food. So the demand for the vegetable has increased recently. This study was carried out to determine the effects of temperature and shading on germination characteristics of A. dioicus. Methods and Results : The experiment was performed by temperature and shading treatments. Seed pre-treatment before the germination experiment was carried out by the storage in low temperature ($4^{\circ}C$) under wet condition (LTW) for 0, 15, 30, 45 and 60 days and shading treatment were 35%, 50%, and 75% under control, BA (6-benzyladenine) and $GA_3$ (gibberellic acid) condition for 24 hours. Increasing the length of the storage periods led to increases seed germination percent in low temperature ($4^{\circ}C$) under wet condition (LTW), germination rate of A. dioicus seed was the highest at $15^{\circ}C$ with 60 days of seed pre-treatment. In the case of seeds pre-treatment with LTW, the more temperature went up, the more days to 50% of Germination of Final Germination Rate ($T_{50}$) went down. As a result of surveying shading treatment, germination rate was the highest in control of 35% shading and the next higher was in control of 50% shading. Conclusions : It is concluded that the temperature and shading are important factors to produce A. dioicus. Also, We suggest these results as basic data of A. dioicus for sexual propagation.

Effects of Semi-dried Red Pepper with a Different Seed Ratio on the Quality of Kimchi (반건조 고추의 씨함량이 김치의 품질에 미치는 영향)

  • Sung, Jung-Min;Lim, Jeong-Ho;Park, Kee-Jai;Jeong, Jin-Woong
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.427-436
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    • 2008
  • This research investigated the application of semi-dried red pepper in Kimchi manufactured using semi-dried red pepper with seed ratios of 20, 40, 60 or 80%. Among the treatments, there were no significant changes in pH, acidity and lactic acid bacterial cell counts during the fermentation period. Kimchi made with a higher pericarp ratio had higher levels of vitamin C, ASTA value and capsainoids during fermentation. The organic acid level showed no significant difference with the various semi-dried red pepper seed ratios, and during fermentation the malic and succinic acid levels decreased while the acetic and lactic acid levels increased. In a sensory evaluation, the overall acceptance was highest for the control group, but the acceptance of Kimchi prepared with semi-dried red pepper at a seed ratio lower than 20% was not significantly different.

Uniform Seedling Establishment and Weed Occurrence Inhibition by Seed-Mulching in Wet Seeded Rice

  • Yang, Woon-Ho;Kim, Jae-Hyun;Kim, Je-Kyu;Han, Hee-Suk;Lee, Moon-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.2
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    • pp.80-84
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    • 2002
  • In spite of simple and cheap cultivation method, water broadcast-seeded rice demonstrates uneven and unstable seedling establishment per unit land area and requires more herbicide and labor-input for weed control. Three experiments were conducted in a phytotron at 18$^{\circ}C$ to evaluate the adaptability of seed-mat mulching cultivation technologies in water seeded rice for the uniform seedling establishment and the inhibition of weed occurrence without herbicide application. Five different kinds of mat with different mesh sizes and fabric properties were tested. The emergence of rice was the highest in Lawn-mat treatment, being comparable with the control, in which seeds were sown without mat. The Lawn-mat also exhibited the lowest mat tension. Mat tension may influence the emergence of rice. And once it was soaked in water, it didn't maintain its original shape any more. The emergence rate of rice was the lowest in Safer-mat treatment. In Lawn-mat treatment, which was the most effective for rice emergence in the first study, the differences of emergence and seedling establishment of rice depending on the seeding position (upper, beneath, and between mats) treatments were negligible, while they were higher in dry seeds than in pre-germinated seeds treatment. The emergence as affected by the kinds of mat also showed the same trend when tested using barnyard grass. Depending on the kinds of mat, the inhibition effect of weeds was the greatest in Safer-mat and the poorest in Lawn-mat. These results strongly suggest the possibility that the uniform seedling establishment and weed management without chemical could be achieved simultaneously by seed-mat mulching through the combination of effective mat for the emergence of rice and another efficient mat for the inhibition of weed occurrence. This possibility was also tested in the field.

Impact of Rhizosphere Competence of Biocontrol Agents upon Diseases Suppression and Plant Growth Promotion

  • Park, Chang-Seuk-
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1994.06a
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    • pp.27-49
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    • 1994
  • Root colonization of biocontrol agents via seed treatment was investigated and a compatible combination, Gliocladium virens G872B and Pseudomonas putida Pf3, in colonizing cucumber rhizosphere was confirmed through the study. Much higher number of fungal and bacterial propagules were detected when two isolates were inoculated together. The presence of Pf3 in root system was greatly helpful to G872B to colonize at root tip. The mechanism of this phenomenon is partially elucidated through the results of in vitro experiments and the observations of scanning electron and fluorescence microscope. Addition of Pf3 cells resulted earlier germination of G872B conidia and increased mycelial growth. And the more number of germinated conidia on seed coat, the more vigorous hypal streching and sporulation on the root surface were observed in coinoculated treatment. The propagules of G872B on the cucumber root when they were challenged against the pathogenic Fusarium oxysporum, were even higher than that of G872B treated alone, and the magnitude of such a difference was getting grater toward the root ip and the population of F. oxysporum on the root was reduced by seed inoculation of G872B. The rhizosphere competence was obviously reflected to disease suppression and plant growth promotion that induced by the given isolate. Green house experiments revealed that the combined treatment provided long-term disease suppression with greater rate and the larger amount of fruit yield than single treatments. Through this study the low temperature growing Pseudomonas fluorescens M45 and MC07 were evaluated to apply them to the winter crops in field or plastic film house. In vitro tests reveal that M45 and MC07 inhibited the mycelial growth of Pythium ultimum, Rhizoctona solani and Phytophthora capsici and enhanced growth of cucumber cotyledon in MS agar. This effect was more pronounced when the bacteria were incubated at 14$^{\circ}C$ than at 27$^{\circ}C$. And disease suppression and plant growth promotion in green house were also superior at low temperature condition. Seed treatment of M45 or soil treatment of MC07 brought successful control of damping-off and enhanced seedling growth of cucumber. The combined treatment of two isolates was more effective than any single treatment.

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Growth characteristics of oyster mushroom in bottle cultivation with addition of cottonseed meal (느타리버섯 재배에서 면실박의 첨가비율에 따른 생육 특성)

  • Lee, Chan-Jung;Lee, Eun-Ji;Park, Hye-Sung;Kong, Won-Sik
    • Journal of Mushroom
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    • v.17 no.3
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    • pp.162-166
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    • 2019
  • This study was carried out to investigate the optimum level of cottonseed meal in bottle cultivation of oyster mushrooms. Mycelial growth was slightly slower than that of the control at a cottonseed meal level ${\leq}10%$. Pileus diameter and thickness were highest at 14%, and stipe diameter was highest at 12% cotton seed meal. The hardness of pileus and stipe was greatest at 16% cotton seed meal. L-values tended to be lower at low levels of cotton seed meal, but the a- and b-values showed no significant difference among the treatments. The yield of fruiting body was 147.2 g/850 ml in the control and slightly higher at 147.6 g/850 ml using 12% cotton seed meal.