• Title/Summary/Keyword: Seed propagation

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Effect of Solidago altissima L. Extract on Forage Crop Germination

  • Ho-Jun Gam;Yosep Kang;Eun-Jung Park;Ki-Yong Kim;Sang-Mo Kang;In-Jung Lee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.114-114
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    • 2022
  • There are 28 families and 166 species of exotic weeds on agricultural land and among these, 23 families and 80 species of exotic weeds occur on pastures. Among them, the Solidago altissima is a perennial weed belonging to the asteraceae family and it is an exotic weed that spreads to the surrounding area using methods such as high seed production, vegetative propagation using underground rhizomes and allelochemical. Accordingly, in 2009, the Ministry of Environment designated it as an ecosystem-disrupting species. This study was conducted to obtain basic data about the effects of S.altissima derived allelochemicals on forage crops. The root of S.altissima was separated, dried in the shade and then pulverized to prepare an root powder. Powder was repeatedly extracted with methanol for 3 days and concentrated under reduced pressure to obtain an root methanol extract. Dissolve the extract in distilled water, dispense it in a separate-funnel and proceed with liquid-liquid extraction by adding equal amounts of n-haxane (Hex), chloroform (CHCI3), ethyl acetate (EtoAC), and butanol (BuOH) in order of increasing polarity. A seed-bioassay was performed using fractions for each solvent, followed by separation and purification by silica gel column chromatography. As a result of the fraction germination test for each solvent, the IC50 values using the fresh weight of each fraction were 898.3 mg L-1, 676.3 mg L-1, 1160 mg L-1 and 1360 mg L-1. CA, CB, and CC fractions were obtained through primary silica gel column chromatography that used CHCI3 fraction. As a result of seed-bioassay using each fraction, the IC50 values for the fresh weight of each fraction was 537.3 mg L-1, 1280 mg L-1 and 1947 mg L-1. Based on this, 5 fractions were obtained as a result of secondary silica gel column chromatography using the CA fraction. A seed-bioassay was performed, as a result, the lowest IC50 value was calculated as 226.7 mg L-1 in the CAE fraction. Based on this, the fraction was analyzed by GC-MS. The results of this study can be used as basic research data on the effects of weeds on forage crops and allelochemicals secreted from S. altissima.

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Morphological Characteristics and Germination as Affected by Low Temperature and GA in Orostachys 'Jirisan' and 'Jejuyeonhwa' Seeds, Korea Native Plant (지리산바위솔과 제주연화바위솔 종자의 형태특성 및 저온과 GA에 대한 발아 반응)

  • Kang, Jeong-Hee;Jeong, Kyeong-Jin;Choi, Kyoung-Ok;Chon, Young-Shin;Yun, Jae-Gill
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.913-920
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    • 2010
  • This study was conducted to improve the seed germination by low temperature and $GA_3$ in $Orostachys$ $japonicus$ A. Berger (Jirisan) and $O.$ $iwarenge$ (Mak.) Hara (Jejuyeonhwa), Korean native plants. Observation of the seeds using a stereo microscope showed that all seeds of the two species have wrinkled surface and oblong shape. Seed size ranged 0.77-1.00/0.25-0.37 mm (length/width), indicating that the seeds are minute seeds. When the seeds of two $Orostachsis$ species were sown into petri-dish and placed in a plant growth chamber of 10, 15, 20, or $25^{\circ}C$, 'Jirisan' showed seed germination below 20% at all temperatures and 'Jejuyeonhwa' 80% at only $10^{\circ}C$. Seed germination of 'Jirisan' increased up to 44% at $10^{\circ}C$ by low temperature ($4^{\circ}C$) storage for 10 days, but decreased again at storage for more than 20 days. The seeds of 'Jejuyeonhwa' showed a large increase in seed germination by low temperature for 20-30 days, which was 95% at $10^{\circ}C$, but low temperature for more than 40 days significantly decreased seed germination. Dipping treatment in GA3 solution of $50-400mg{\cdot}L^{-1}$ for different periods (3, 6, 12, and 24 hrs) remarkably improved germination rate and speed in both species, 80-100% in 'Jirisan' and 90-100% in 'Jejuyeonhwa' at all concentrations and dipping times used in this study.

Propagation of Cutting Method of a Rare Endemic Wikstroemia ganpi (Sieb. et Zucc.) Maxim. in Korea (희귀수종 거문도닥나무(Wikstroemia ganpi (Sieb. et Zucc.) Maxim.)의 번식에 관한 연구)

  • Yoon, Jung Won;Yi, Myung Hoon;Sung, Jung Won
    • Korean Journal of Plant Resources
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    • v.33 no.4
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    • pp.303-310
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    • 2020
  • This study was conducted on propagation use for conservation scheme of a threatened plant; Wikstroemia ganpi (Sieb. et Zucc.) Maxim(Geomundo false ohelo). The seed propagation was showed higher (95%) in storage and lower values in ground and cutting. Softwood cutting was higher than hardwood cutting and it was possessing higher ratio of rooting that increase concentration of IBA and NAA. It was determined that for Geomundo false ohelo seedling was effective than cutting. In-situ and ex-situ conservation and restoration of substitute habitats of Geomundo false ohelo is therefore necessary due to human trampling in the habitats, damage, natural selection, loss and suppression.

Breeding of 'Seeberry' F1 Hybrid Strawberry (F1 종자 딸기 '씨베리' 육성)

  • Jeong, Ho Jeong;Lee, Sun Hee;Cho, Il Whan;Rho, Il Rae
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.448-452
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    • 2015
  • 'Seeberry' is a short-day $F_1$ hybrid strawberry for seed propagation, that was released by the Protected Horticulture Experiment Station (RDA, Republic of Korea) in 2013. Inbred lines derived from domestic germplasms and selected varieties were used to develop this cultivar. 'Seeberry' originated from a cross of two inbred lines: a male parent 'Wongyo 3115' with high firmness and a female parent 'Wongyo 3116' with excellent fruit shape and high yield. 'Seeberry' cultivar should be grown with a crown diameter of over 10 mm to reveal its unique characteristics. 'Seeberry' has an upright plant shape with elliptic leaves, 12-15 flowers per cluster, vigorless growth habit, and average flower bud differentiation compared to vegetatively propagated cultivars. Fruits of 'Seeberry' are conical, having light skin color and approximately 15-16 g in average weight. Yield of this cultivar is about 92% lower than that of 'Akihime', but 'Seeberry' has excellent taste, sugar content of $9.7^{\circ}Bx$, acidity of 0.6%, and good texture. With regard to disease and pest resistance, 'Seeberry' is sensitive to powdery mildew, aphids, and the two-spotted spider mite, and is resistant to anthracnose.

Production of Carpet Sod Using Seed and Runner of Zoysiagrass (Zoysia spp.) (한국잔디(Zoysia spp.)의 종자 및 영양체를 이용한 carpet 잔디 생산)

  • 최준수;양근모;김동섭
    • Asian Journal of Turfgrass Science
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    • v.15 no.2
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    • pp.39-50
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    • 2001
  • This research was designed to develop the rapid propagation technology of zoysiagrass using carpet production and to provide turf-growing farmers with efficient cultivation methods thor carpet production. Turfgrass cultivar tested in cadet production by seeding was Z. japonica cv. Zenith. Since the surface coverage rate reached to 85% in 2 months after seeding at the rate 6g/$m^2$ in the early part of July, carpet production using ‘Zenith’seeds would be possible to produce within within the same year. Thrfgrasses tested in carpet production by vegetative propagation were Z. japonica, medium type Z. matrella, fine type Z. matrella, and medium type Z. japonica. Planting rates of vegetative parts (sprigs) were 1.2L/$m^2$, 2.4L/$m^2$, 2.5L/$m^2$, and 5L/$m^2$. Two different sizes of sprig were used; 1~2 nodes and 3~4 nodes. Surface coverage rate was 90% in one year after planting at the rate of 2.5L sprigs of medium type Z. japonica. Therefore, the use of sprigs with 3~4 nodes at the planting rate of 2.5L would be suitable for the carpet prodcution. Three months old zoysiagrass carpet (Zenith) was overseeded with Kentucky bluegrass, perennial ryegrass, and tall fescue at two different overseeding rate. Surface coverage rate was the highest (80%) at the plot overseeded with perennial ryegrass that showed the earliest germination. Suitable overseeding species for the rapid sod formation at the early stage of establishment were tall fescue and perennial ryegrass and desirable overseeding time was from early to middle of September.

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Spring Green-up and Winter Leaf Discoloration of Three Ornamental Grasses (Gramineae spp.) (화본과 관상용그래스 3종의 봄철 그린업과 겨울철 엽색 퇴화)

  • Kim, Ki-Dong;Lee, Jeong-Ho;Joo, Young-Kyoo
    • Weed & Turfgrass Science
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    • v.4 no.1
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    • pp.49-57
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    • 2015
  • This research was initiated to analyze the periods of spring green-up and winter leaf discoloration of three ornamental grasses which have potential to be widely used with seed propagation. Two native grasses of Arundinella (Arundinella hirta var. ciliata Koidz), fountaingrass (Pennisetum alopecuroides (L.) Spreng), and switchgrass (Panicum virgatum L.) was tested. Spring green-up were evaluated after one year growth from seed propagation on April 1, 2009. Arundinella started with quick pick of spring green-up during $13^{th}$ to $20^{th}$ of May. Fountaingrass and switchgrass showed relatively slow picks of green-up during $20^{th}$ to $27^{th}$ of May. However, winter leaf discoloration started on swtichgrass and fountaingrass but Arundinella terminated relatively slowly. Swtichgrass showed the pick discoloration during $8^{th}$ to $15^{th}$ of October from the bottom to top parts of the plant. Fountaingrass showed the pick winter discoloration started from bottom to top parts during the $22^{nd}$ to $29^{th}$ of October. However, Arundinella showed relatively slow discoloration from upper to bottom parts during October $29^{th}$ to November $5^{th}$. Arundinella showed a relatively higher ornamental value with 125 days of the complete green period compared than fountaingrass and swtichgrass which maintained approximately 105 days of green period.

Characteristics of Seedling and Rhizome Propagation in Epimedium koreanum NAKAI (삼지구엽초 실생 및 지하경번식 특성)

  • Noh, Joon-Hyun;Kim, Young-Jin;Choi, Kang-Jun;Kim, Se-Won;Kim, Sung-Kyeong;Kim, Jong-Hwa
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.2
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    • pp.155-160
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    • 2003
  • This study was carried out to investigate propagation characteristics of seedling and rhizome in Epimedium koreanum NAKAI. Seed germination essentially needed after-ripening duration after harvesting for 250days and more by stratification. At $20^{\circ}C$ seed emergence days and root length were 8 and 0.46cm respectively. The above-ground part of E. koreanum grew until 60days after root cutting and the underground part of it grew after that time. The optimum amount of organic fertilizer was fermented cow-manure with husk 2,000kg/10a that showed $57.5cm^2$ in leaf area per plant, 6.2cm in new rhizome length, and 0.43% in icariin content, an index component of E. koreanum, while inorganic N-fertilizer made E. koreanum worse as it increased. In nutriculture extruded rice hull media was better for growth than perlite media at 1/2 NHRI solution.

Research on Ginseng Production During the Past 20 years (인삼재배 분야의 과거 20년 연구)

  • Park, Hoon
    • Journal of Ginseng Research
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    • v.20 no.4
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    • pp.472-500
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    • 1996
  • Researches on mineral nutrition, physiology and phyrsiological diseases, . cultivaction methods. brceding. pest control quality management and extension during 1976-1995 in Korea were reviewed Review in brceding and pest control was restricted to the researches directely related to cultivaction. Mineral nulrient up take. partion and varicos factors such as top dreasing. Light intersity etc. and interrelationship between minerals were investigated. Top dressing was not effective due to low minera1 requorement Physiological characteristics on tempelature light and water were well elucidated and applied to assess traditional cultivation method and its inovation. Photosyrnthetic pigments. light harvest proteins and activity of related enzymes were studied. In nitrogen metabolism arginine, praline, ammonium, threonine appeared to have important role in re growth of shoot Saponin metabolism was studied in relation to growth and new ginsenosides were found but physiological role of saponin was not clearly elucidated yet Endogenous growth regulators were reported and various erogenous growth regulators were studied for growth stimulation. short stem and seed pruning etc. Various physiological diseases were investigated for cause and control measures were established. Water culture was little studied Forest culture was studied but not retched the recommendable stage Drip irrigation straw mulching. seasonal shading and soil preparation method including soil fertility adjustment were established for practical application. Shading materials completely changed to polyethylene net and materials of polymers The research on ginseng cultivation in paddy field opened the way to establish the permanent ginseng cultivation plantation Ginseng harvester and seeder were developed in the late 1950s. Transplanted and many other machines were developed in the early 1990s. In ginseng breeding only pure line selection was of practical significance several verities were at the stage of seed propagation at ginseng plantations. Mutation breeding (${\gamma}$-ray. X-ray chemicals) was not successful. The research on plantlet formation through tissue culture was a little progressed but still far behind to vegetative propagation. Disease control research was concentrated in the isolation and identification of pathogans. their ecological charactelistics and biological control and soil humigation. Potato root rot nematodes was found and control method was established. Insect and small animal control research was greatly progresses in identification, ecological investigation, and ecological and physical control. Weed control was less important due to the development of mulching method of ridge and ditch. Quality factors of raw ginseng in relation to red ginseng process were extensively studied. Traditional quality measures were elucidated in accordance with modern analytical chemistry resulting in the importance of peptides in the centrat part rather than ginsenosides For large root production growth promoting rootzone micrcorganisms (PGPRM) were isolated and active compounds were identified. Field test on PGPRM was on going. Varictus methods formality improvement through cultivation were developed. Management research of ginseng production was rare Extension was active throuch official and private organizations and through workshop for the extension specialists, and direct lectures to grower's. Extension services made the researcher to understand the existing problems at grower's fields. Research environment for ginseng production was in prime time only for three years when Korea Ginseng Research Institute was established then gradually aggravated.

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Effects of Elevated CO2 and Temperate on the Growth of Endangered Species, Cicuta virosa L. in Korea (CO2농도와 온도 상승이 한국멸종위기식물 독미나리의 생장에 주는 영향)

  • Park, Jae Hoon;Hong, Yong Sik;Kim, Hae Ran;Jeong, Jung Kyu;Jeong, Heon Mo;You, Young Han
    • Journal of Wetlands Research
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    • v.16 no.1
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    • pp.11-18
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    • 2014
  • The effect of elevated $CO_2$ and temperature on ecological characteristics of Cicuta virosa L., the endangered plant were examined under ambient $CO_2$+ambient temperature(AC-AT), ambient $CO_2$+elevated temperature(AC-ET) and elevated $CO_2$+elevated temperature for two years. Shoot length and the number of umbels were not different in three environmental gradients. The number of tillers was high in the order of EC-ET, AC-ET and AC-AT. The number of compound umbel was the lowest in the EC-ET. Fruit set rate was the highest in the AC-AT. These results mean that unsexual propagation of C. virosa may increase by promoting growth of tillers, rather than seed production under future global warming. This population growth study will be used as the important data for the research of Korean endangered species.

A Study of Modified Parallel Feistel Structure of Data Speed-up DES (DES의 데이터 처리속도 향상을 위한 변형된 병렬 Feistel 구조에 관한 연구)

  • Lee, Seon-Keun;kIM, Hyeoung-Kyun;Kim, Hwan-Yong
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.12
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    • pp.91-97
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    • 2000
  • With the brilliant development of information communication and the rapid spread of internet, current network communication is carrying several up-to-date functions such as electronic commerce, activation of electro currency or electronic signature and will produce more advanced services in the future. Information communication network such as that electronic commerce would demand the more safe and transparent guard of network, and anticipate the more fast performance of network. In this paper, in order to meet the several demands, DES(data encryption standard) with parallel feistel structure, which feistel structure of the basic structure of DES is transformed into in parallel, is proposed. The existing feistel structure can't use pipeline method for the structural problem of DES itself-the propagation of error. therefore, this modified parallel feistel structure could improve largely the performance of DES which had to have the trade-off relation between data processing speed and data security and in addition a method proposed in SEED having adopted the modified parallel feistel structure shows more excellent secure function and/or fast processing ability. The used CAD Tool use Synopsys Ver. 1999. 10 in both of synthesis and simulation.

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