• Journal of Environmental Science International
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• v.24 no.12
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• pp.1673-1679
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• 2015

This study was conducted to investigate the effects of fruit set internode on the germination of watermelon seeds. Generally fruits setted in higher than 20 internode were high percent of germination, fruit setted in low internode(5-10) is low. The higher fruit-set internode, fruit weight was significantly increased, but 1,000 seed weight was decreased the more fruit set internode. However number of seed was about 300 seeds irrespective of fruit-set internode. Investigating viability of seed by BP test, general percent germination was recorded highest at 5 flower cluster above 20 internode of fruit-set internode. In contrast, condition of fruit setted on 3, 4, 6 flower cluster were high percent of germination, regarded as unnormal germination not to be as normal seedling by 2~3%. Seed harvested at 5 flower-cluster had high viability on hypocotyl height and diameter of seedling through early growth test, but were not significant. Therefore 5 flower-cluster was optimum fruit-set internode to obtain high-quality seed.

• Journal of Environmental Science International
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• v.11 no.4
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• pp.319-325
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• 2002

This experiment was carried out to investigate the effects of heavy metals (copper, cadmium, lead and chrome) on the growth of plant and seed germination of Arabidopsis thaliana treated with various concentrations of heavy metals. Cadmium and chrome among the 4 heavy metals had no effect on the growth of stem even in the concentration fifty times higher than the official standard concentration of pollutant exhaust notified by the Ministry of Environment. The official standard concentration of cadmium, however, stimulated the growth of stem in general, increasing leaf size and surface area, although it had no effect on the length of stem. But the growth of stem was decreased about 18% in the official standard concentration of pollutant exhaust of lead and copper. There was no growth of root in the concentration of lead and copper ten times higher than the official standard concentration. Cadmium and chrome had no effect on the seed germination, but lead and copper decreased the rate of seed germination. Seeds were not germinated in the concentration of copper ten times higher than the official standard concentration and in the concentration of lead fifty times higher than the official standard concentration. From this research three peculiar results were obtained. Chrome in the soil did not have much effect on the plant growth and seed germination of Arabidopsis thaliana. Cadmium stimulated the stem growth in an optimum concentration. But lead and copper reduced the plant growth and seed germination even in a small concentration, especially copper had the worse effect.

• Korean Journal of Environmental Agriculture
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• v.28 no.4
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• pp.403-408
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• 2009

Dandelion (Taraxacum platycarpum) has been widely utilized for medicinal purposes. However, the dandelion seeds are relatively difficult to germinate under cultivation conditions, which hampers seedling propagation of dandelion plants and reduces the opportunity of usage of such a useful medicinal plant. Thus, in this study, in vitro conditions for the dandelion seed germination were optimized to enhance the germination rate. In seed washing steps, the sequential treatments with 20% of ethanol, 20% of NaOCl, and distilled water avoided microbial contamination with the highest in vitro germination rate (67.5%) from seeds sown in germination media. The media supplemented with 1.4 g/L of MS salts and 1% of sucrose significantly enhanced the germination rate compared to the media with 4.4 g/L of MS and 3% of sucrose. Sowing the seeds vertically in the optimized media supplement conditions, 1.4 g/L of MS salts and 1% of sucrose, gave the maximum in vitro germination rate (61%), which was almost three times higher than sowing seeds on a soil pot (23%). Our results indicate that the seed washing and sowing methods including germination medium supplements can be optimized to enhance in vitro seed germination of dandelion.

• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.317-324
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• 2007

This study was carried out to obtain the several data for improving the seed supply in Pinus densiflora. Seed characteristics and germination properties according to change of cone production from 2003 to 2006 were analyzed in three plus stands of P. densiflora. P. densiflora showed good production at interval of two years in the periodicity of seed production. Seed potential and percent of filled seed (PFS) among the major seed characteristics showed positive relations to the change of cone production, whereas percent of developed seed (PDS) and seed efficiency showed opposite trend. As a result of regression analysis between seed characteristics and cone production, seed potential ($R^2=0.682$, p<0.01) and PFS ($R^2=0.161$, non-significant) presented positive correlation with cone production while PDS ($R^2=0.418$, p$R^2=0.308$, non-significant) presented negative correlation. In germination properties percent of germination (PG) showed similar tendency to cone production, while mean germination time (MGT) showed opposite tendency. As a result of regression analysis between germination characteristics and cone production, PG showed positive correlation, whereas MGT showed negative correlation. Therefore, we could expect high germination percentage and short period for germination when we harvested cones at good cone production time.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.6
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• pp.38-51
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• 2006

This study was carried out to improve rehabilitation effect by hydro-seeding methods on denuded slopes. Lespedeza cyrtobotrya, Indigofera pseudotinctoria, Arudinella hirta, Poa pratensis, and Lolium perenne were used in this study. The results of the germination and growth characteristics by seed coating, slope control and soil mulching treatments are summarized here. Seed coating of those plants had an effect on germination ratio, germination force, $LD_{50}$, and seedling growth of stem, leaf and root. The seed coating effect was highest for I. pseudotinctoria while the seedling growth was best for L. cyrtobotrya. Vermiculite+Talcum was the best coating material for germination and seedling growth of the plants. As the slopes were steeper, germination ratio was lower and seedling growth of stem and root decreased. Lots of seeds and soils were swept away when the slope was steep. Soil mulching was effective for germination, seedling growth of stem and root, and soil stabilization. It was more effective when the slope was steeper. Coir net was the most effective soil mulching material n this study.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.1
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• pp.153-163
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• 2015

The study was conducted to develop ecological restoration method of damaged area in DMZ vicinities. As the material for the ecological restoration, forest soil and trees waste have been used. Forest soils were collected for experiments in Yanggu, Gangwon Province. Effect of ecological restoration was analyzed through germination experiment. Germination experiment was performed using 12 kinds of woody and herbaceous seeds. Woody and herbaceous seed germination in test pot was relatively evenly. Mixed material consisting of forest soil and trees waste seemed a possibility as the material for the restoration. The effects on seed germination were higher in the case of mixing more than 70% by volume ratio of local resources. Total number germinated individuals were different depending on the mixing ratio of soil sampling depth. Individual plants showed different trends depending on the experimental combination. Results of the woody seed germination were affected only in the soil sampling depth. Seed germination of herbaceous received a combined effect on soil sampling depth and mixing ratio.

• Plant Resources
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• v.6 no.2
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• pp.94-97
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• 2003

The objective of this study is to investigate morphological characteristics of seeds and effects of environmental factors such as light and temperature on the germination and seedling growth of Adenophora triphylla var. japonica, Codonopsis lanceolata, Platycodon glandiflorum, Which belongs to Campanulaceae and useful for on oriental medicine materials. Seed shape of Codonopsis lanceolata is Elliptic. Platycodon glandiflorum is Oblong. Color of seed coats varied from yellow to dark brown. Seed size of Adenophora triphylla var. japonica is 1.37mm in length, 0.69mm in width. One thousand-seed weight of Adenophora triphylla var. japonica is 258mg. Seed size of Codonopsis lanceolata is 4.45mm in length, 2.11mm in width. One thousand-seed weight of Codonopsis lanoceolata is 1,278mg. Seed size of Platycodon glandiflorum is 2.31mm in length, 1.10mm in width. One thousand-seed weight of Platycodon glandiflorum is 988mg. Optimum temperatures for seed germination and seedling growth range from 20 to $25^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.427-434
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• 2014

This study aimed to investigate the effects of pre-harvest sprouting (PHS)-damaged grains of rice on seed viability, germination percentage and seedling emergence rate. As comparing the seed lots of same cultivar 'Hopum' with different PHS percentages, seed lot with 8% PHS (high PHS percentage) showed 30% lower germination than that with 1% PHS (low PHS percentage). The difference of seedling emergence rate and seed viability percentages between the two seed lots was consistent with that of germination percentage. PHS-damaged brown rice was observed protruded embryo, discolored endosperm and fungi infected grains. PHS grains were classified into two groups, PHS-I showing endosperm discoloration in one third of a grain and PHS-II in more than half of a grain. The seed lot with high PHS percentage had much more PHS-II grains than that of low PHS percentage. PHS-II grains showed remarkably lower germination percentage than PHS-I grains and got severely moldy during germination test. In laboratory experiment, morphological changes and re-germination ability of seeds which dried after germination by soaking were tested. Sprouted seeds of 3mm shoot length showed half discolored endosperm and had a strong resemblance with PHS-II grains. Re-germination percentage of 3mm sprouted seeds sharply decreased than 2 mm sprouted seeds. Sprouted seeds which were treated for accelerated-aging (AA) for 24hr at $45^{\circ}C$ were significantly reduced in re-germination percentage compared to that of non-AA-treated sprouted seeds. These results indicate that PHS with over 3mm shoot length can severely damage not only grain appearance but also seed viability, germination percentage and seedling emergence rate. Therefore, we conclude that larger difference of germination percentage or seedling emergence rate between seed lots having smaller difference of PHS percentage might be caused by accelerated seed deterioration in PHS-damaged rice grains.

• Korean Journal of Medicinal Crop Science
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• v.22 no.1
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• pp.53-59
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• 2014

This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed germination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a result the highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours in $dH_2O$. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in room temperature $2^{\circ}C$. Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). In this research, T. kirilowii and T. kirilowii var. japonica seed were stored in room temperature or $2^{\circ}C$, and then sown in peat moss, seed germination rate was more than 90% and production of superior quality seedlings.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.74-77
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• 1999

The object of this study is to determine the effect of priming on the germination ability and seedling emergence of aged soybean seeds in lab and field conditions. Artificial or natural procedure for seed aging was applied in this study. One seed lot was artificially aged for 3 to 5 days at 42$^{\circ}C$ with high relative humidity (nearly RH 100%), and the other one was stored at room temperature for 17 months. Aged seeds were osmoconditioned in -1.1 MPa polyethylene glycol 8000 (PEG) solution for 3 days at $25^{\circ}C$ and air-dried. When Danyeobkong was aged for 4 days average germination was 61.5%, however, this improved to 98.5% after the priming treatment. Improvement of seed germination by priming the aged seed was consistent with large seed sized Jangyeobkong cultivar, indicating that the priming was effective in enhancing seed germinability regardless of seed size. Priming aged seeds also resulted in good stand establishment in the field trials. Germination of aged seeds of Danyeobkong without priming was 17.0%, whereas that of primed ones was 66.4%.