• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.348-352
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• 1994

Soybean seed consists of two major storage protein, the 7S and 11S globulins. For improving the quality of soybean seed protein, an increase of 11S/7S ratio would be a desirable objective because the 11S globulin contains much more the sulfur-containing amino acids than the 7S globulin. In this study, some soybean varieties were used to investigate the analyzing method for 7S and 11S globulins. 7S and 11S globulins couble be fractionated by their different solubilities in tris buffers. Adjusting the pH and tris concentration were major factors affecting the precipitation of the two globulins. And it was possible to screen the soybean genotypes having aberrant subunit compositions of the two globulins by an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soybean proteins. The ratio of 11S to 7S globulin ranged from 1.29 to 1.38. This paper also dealed with the contribution of protein components in soybean seeds to the physical properties of soycurd. It indicated that the soycurd from crude 11S was remarkably harder than that from crude 7S, and springiness and cohesiveness were slightly higher in soycurd having higher proportion of 11S. So, it may concluded that proportion of protein components in soybean seed can be important factor which controls the suitability for soycurd or other foods.

• Journal of Venture Innovation
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• v.5 no.3
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• pp.1-17
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• 2022

Entrepreneurs recognize that their passion was an important factor in driving successful entrepreneurship. However, they were often unaware of the impact that third-party passions related to startups have on them. Therefore, in this study, it was verified whether the accelerator incubation program had an effective effect on portfolios. At this time, I tried to do an empirical analysis focusing on how the third-party accelerator entrepreneur passion affects the entrepreneurial performance of portfolios. To this end, a survey was conducted on representatives of portfolios across the country who completed the accelerator incubation program, and empirical testing was conducted based on 330 valid ones. As a result of empirical analysis, it was confirmed that mentoring and networking among accelerator incubation programs had a positive (+) effect on entrepreneurial performance of portfolios. On the other hand, education and seed investment in the accelerator program did not significantly affect the entrepreneurial performance of portfolios. On the other hand, it was tested that accelerator entrepreneur passion significantly moderated both the incubation program elements (education, mentoring, network, seed investment) and the entrepreneurial performance of portfolios. The results of this study are meaningful in that they reveal that the passion of accelerator entrepreneurs is an important lynchpin of incubation programs and the key to success in startups. In addition, this study suggests that it is important for startups to go one step further from seed investment and properly identify the accelerator entrepreneur and his passion, the key man of accelerators.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.1
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• pp.1-8
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• 2017

We investigated the effect of silicate coating of rice seeds on bakanae disease incidence and the quality of seedlings raised in seedling boxes and transplanted into pots. The silicate-coated rice seed (SCS) was prepared as follows. Naturally infested rice seeds not previously subjected to any fungicidal treatment were dressed with a mixture of 25% silicic acid at pH 11 and 300-mesh zeolite powder at a ratio of 50 g dry seed - 9 mL silicic acid - 25 g zeolite powder. The following nursery conditions were provided : Early sowing, dense seeding in a glass house with mulching overnight and no artificial heating, which were the ideal conditions for determining the effect on the seed. The nursery plants were evaluated for Gibberella. fujikuroi infection or to determine the recovery to normal growth of infected nursery plants in the Wagner pot. Seedlings emerged 2-3 days earlier for the SCS than they did for the non-SCS control, while damping-off and bakanae disease incidence were remarkably reduced. Specifically, bakanae disease incidence in the SCS was limited to only 7.8% for 80 days after sowing, as compared to 91.6% of the non-SCS control. For the 45-days-old SCS nursery seedlings, the fresh weight was increased by 11% and was two times heavier, with only mild damage compared to that observed for non-SCS. Even after transplanting, SCS treatment contributed to a lower incidence of further infections and possibly to recovery of the seedlings to normal growth as compared to that observed in symptomatic plants in the pot. The active pathogenic macro-conidia and micro-conidia were considerably lower in the soil, root, and seedling sheath base of the SCS. In particular, the underdeveloped macro-conidia with straight oblong shape without intact septum were isolated in the SCS ; this phenotype is likely to be at a comparative etiological disadvantage when compared to that of typical active macro-conidia, which are slightly sickle-shaped with 3-7 intact septa. A active intact conidia with high inoculum potential were rarely observed in the tissue of the seedlings treated only in the SCS. We propose that promising result was likely achieved via inhibition of the development of intact pathogenic conidia, in concert with the aerobic, acidic conditions induced by the physiochemical characteristics associated with the air porosity of zeolite, alkalinity of silicate and the seed husk as a carbon source. In addition, the resistance of the healthy plants to pathogenic conidia was also important factor.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.25-25
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• 2014

Three species of Fusarium, F. fujikuroi, F. verticillioides and F. proliferatum, are known to be associated with bakanae disease of rice [1, 2]. F. fujikuroi infects rice flowers and survive in endosperm and embryo of the seeds. Infected seed is an important source of primary inoculum of pathogens [3]. Seeds of rice (Oryza sativa cv. Boramchan) collected from bakanae-infected field were found to be 96% infected with Fusarium sp., 52% with F. fujikuroi, 42% with F. verticillioides, and 12% with F. proliferatum as determined by incubation method and species-specific PCR assays. F. fujikuroi was detected at lemma/palea, endosperm and embryo whereas F. verticillioides and F. proliferatum were recovered only from lemma/palea by means of component plating test. Seed disinfection methods have been developed to control bakanae disease and prochloraz has been most widely used for rice seeds. Two chemicals formulated with prochloraz (PC 1) and prochloraz + hexaconazole (PC 2) that inhibit biosynthesis of ergosterol strongly reduced the incidence of Fusarium spp. on selective media to 4.7% and 2.0%, respectively. Disease symptoms of rice seedlings in nursery soil were alleviated by chemical treatment; seedlings with elongated leaves or wide angle between leaf and stem were strikingly reduced from 15.6 to 3.2% (PC 1) and 0 (PC 2), stem rots were reduced from 56.9 to 26.2% (PC 1) and 32.1% (PC 2), and normal seedling increased from 0.4 to 13.3% (PC 2). Prochloraz has some disadvantages and risks such as the occurrence of tolerant pathogens [4] and effects on the sterol synthesis in animals and humans [5]. For these reasons, it is necessary to develop new disinfection method that do not induce fungal tolerance and are safe to humans and animals. Chlorine dioxide ($ClO_2$), that is less toxic, produces no harmful byproducts, and has high oxidizing power, has been reported to be effective at disinfection of several phytopathogenic fungi including Colletotrichum spp. and Alternaria spp. [6]. Gaseous $ClO_2$ applied to rice seeds at a concentration of 20 ppm strongly suppressed mycelial growth of Fusarium fujikuroi, F. verticillioides and F. proliferatum. The incidence of Fusarium spp. in dry seed with 8.7% seed moisture content (SMC) tended to decrease as the concentration of $ClO_2$ increased from 20 to 40 ppm. Applying 40 ppm $ClO_2$ at 90% relative humidity, incidence was reduced to 5.3% and resulted in significant reduction of disease symptoms on MS media. In nursery soil, stem rot was reduced from 56.9 to 15.4% and the number of normal seedlings increased from 0.4 to 25.5%. With water-soaked seeds (33.1% SMC) holding moisture in the endosperm and embryo, the effectiveness of disinfection using $ClO_2$ increased, even when treated with only 20 ppm for four hours. This suggests that moisture was a key element for action of $ClO_2$. Removal of the palea and lemma from seeds significantly decreased the incidence of Fusarium spp. to 3.0%. Seed germination appeared to decrease slightly by water-soaking at $30^{\circ}C$ because of increased SMC and by physical damage of embryos from hulling. These results indicate that the use of gaseous $ClO_2$ was effective as a means to disinfect rice seeds infected with Fusarium spp. and that moisture around the pathogens in the seed was an important factor for the action of $ClO_2$. Further investigations should be conducted to ascertain the best conditions for complete disinfection of Fusarium spp. that infect deep site of rice seeds.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.1
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• pp.37-48
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• 2024

Songla (Usnea diffracta Vain.) is one of the lichens belonging to the genus Usnea, and pharmacological activities such as antioxidant, antimicrobial, anti-inflammatory, anti-tumor and cardiovascular protection have been reported in previous studies, but its efficacy in skin and hair is not well known. In this study, the effect of Usnea diffracta extract (UDE) on anti-aging and dermal papilla cell proliferation was verified in vitro. As a result of the experiment, it was confirmed that the UDE significantly reduced the expression of MMP-1 and the activity of MAPKs (ERK, p38, JNK) and AP-1 (c-Fos, c-Jun), which were increased by UVA in HDFn. In addition, the UDE significantly increased the proliferation of HFDPC and significantly increased the mRNA expression of VEGF and KGF, which are hair growth factors. Accordingly, the phosphorylation of ERK/CREB involved in hair proliferation and expression of growth factors was increased in a concentration-dependent manner. The main component represented by the main peak was separated and purified using Prep LC by concentrating the UDE, which was confirmed as diffractaic acid through NMR and Mess analysis. Isolated diffractaic acid significantly reduced the expression of MMP-1 increased by UVA in HDFn and increased the proliferation of HFDPC in a concentration-dependent manner. The result suggest that UDE proved its usability as a natural cosmetic material with anti-aging and dermal papilla cell activation effects.

• Molecules and Cells
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• v.40 no.4
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• pp.254-261
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• 2017

Glioblastomas (GBM) are very difficult to treat and their aggressiveness is one of the main reasons for this as well as for the frequent recurrences. MicroRNAs post-transcriptionally regulate their target genes through interaction between their seed sequence and 3'UTR of the target mRNAs. We previously reported that miR-296-3p is regulated by neurofibromatosis 2 (NF2) and enhances the invasiveness of GBM cells via SOCS2/STAT3. In this study, we investigated whether miR-296-5p, which originates from the same precursor miRNA as miR-296-3p, can increase the invasiveness of GBM cells. It was observed that miR-296-5p potentiated the invasion of various GBM cells including LN229, T98G, and U87MG. Through bioinformatics approaches, two genes were identified as miR-296-5p targets: caspase-8 (CASP8) and nerve growth factor receptor (NGFR). From results obtained from Ago2 immunoprecipitation and luciferase assays, we found that miR-296-5p downregulates CASP8 and NGFR through direct interaction between seed sequence of the miRNA and 3'UTR of the target mRNA. Knockdown of CASP8 or NGFR also increased the invasive ability of GBM cells, indicating that CASP8 and NGFR are involved in potentiation of invasiveness by miR-296-5p. Consistent with our findings, CASP8 was downregulated in brain metastatic lung cancer cells, which have a high level of miR-296-5p, compared to parental cells, suggesting that miR-296-5p may be generally associated with the acquisition of invasiveness. Collectively, our results implicate miR-296-5p as a potential cause of invasiveness in cancer and suggest it as a promising therapeutic target for GBM.

• Journal of Korean Society of Forest Science
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• v.80 no.3
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• pp.265-278
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• 1991

The purpose of this study is to suggest objective basic data for the design and management of the national park roadside slopes through the quantitative analysis of the visual quality included in the physical environment of the Chi-ri national park, for this, visual volumes of physical elements have been evaluated by using the mesh analysis, spatial images structure of physical elements have been analyzed by factor analysis algorithm, and degree of visual quality have been measured mainly by questionnaires. Result of this thesis can be summarized as fallows. Visual volumes of the naked, rock, ground cover of seed spray, and artificial planting are found to be the main factor determining the visual quality. Factors covering the spatial image of the national park roadside slopes landscape have been found to be the overall synthetic evaluation, spatial, appeal, physical, openness and dignity factors such as the overall the spatial, physical and openness yield high factor scores. As for the factors determining the degree of visual quality of the roadside slopes, variables such as the summit, the constructions management, harmony of landscape, visual stability of roadside slopes, suitable artificial planting and suitable constructions.

• International Commerce and Information Review
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• v.13 no.3
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• pp.249-274
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• 2011

This paper examines agri-food export competitiveness among 4 countries(Korea, USA, Japan, China) based on Porter's Diamond model approach. Korea agri-food competitiveness index is much less than those of other countries. Korea is the lowest ranked among the 4 countries in terms of three determinants(except 1 factor, related and supporting industries) of Porter's Diamond Model: Factor condition, demand condition, firm strategy, structure and rivalry. Korea is assumed to have weak point in the factor condition, demand condition because of low capital and land as well as insufficient investment on the agri-food sector. on the other hand, it seems to have strong factor in consumer condition(demand condition). This paper suggest the following strategies to reinforced the agri-food export sector in Korea. 1) an incentive system for seed development and capital investment, 2) a cluster strategy between agriculture and related industry.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.05a
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• pp.26-27
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• 2014

Sustainable development has become a growing concern throughout the world. Construction industry also has participated in the efforts to minimize the environmental impacts. In Korea, the government has already established the green construction certification(G-seed) and guidelines for sustainable construction. However, domestic guidelines are focused on the design and construction phase, not on the construction project. In addition, it is difficult to find instructions due to the complex structure based on the work unit. Therefore this study aims to deduct the component structure for sustainable construction guidelines in the planning, design and construction phases by utilizing the Factor Analysis. Following the literature review, a total of 26 criteria were identified. Further, a survey was conducted to capture the perceptions on the importance of the criteria. According to the factor analysis, the criteria can be grouped into 8 factors : Environmental factors management, Management planning, Cost and Design management, Energy management, Material management, Certification and regulation management, General Management and Others.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.1528-1537
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• 2005

The purpose of this study is investigated the method for estimation of the liquefaction on the reclaimed land, located in Incheon and assessed the ability of liquefaction under the condition of criteria, which is the magnitude '6.5' of seismic on the basis of the domestic seismic characteristics. Performed not only field test but the experiment as well to study how the fine content would affect into the dreging and reclaimed land and also estimated the safety factor through the empirical method and anticipated detail method based on the results. Within the reclaimed land, there are many sized soils which are almost extended from well-graded silty sand(SM) to poor-graded fine grained sand, and which have the condition, so called, the liquefaction which is easily to bring into under the circumstances within the ground. However, partly, now that the soil is mixed with silt and silty clay which include the content of fine grained dust quite a bit, the difficulties and inconveniences has been expected while trying to find the ratio of cyclic resistance, but finally Seed and Idriss method showed the most way when we estimate the safety factor on the liquefaction.