• The Plant Pathology Journal
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• 제20권4호
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• pp.302-307
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• 2004

Three pairs of specific primers were developed for rapid and precise RT-PCR detection of three seed-transmissible viruses, namely Peanut clump virus (PCV, Pecluvirus), White clover mosaic virus (WCIMV, Potexvirus) and Carrot red leaf virus (CaRLV, Luteovirus). Each primer set was found in conserved region through multiple sequence alignment in the DNAMAN. Total nucleic acids extracted from PCV-, WCMV-, and CaRLV-infected seeds and healthy plants were used for RT-PCR detection using each virus-specific primer, Sizes of PCV, WCIMV, and CaRLV PCR products were 617bp (PCV-uni5 and PCV-uni3 primers), 561bp (WCMV-CP5 and WCMV-CP3 primers), and 626bp (CL1-UP and CL2-DN primers); which corresponded to the target sizes. Nucleotides sequences of each amplified cDNA were confirmed which belonged to the original virus. This study suggests that these virus-specific primer sets can specifically amplify viral sequences in infected seeds. Thus, they can be used for specific detection of three viruses (PCV, WCMV and CaRLV) from imported seed samples for plant quarantine service.

• 한국식물병리학회지
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• 제2권2호
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• pp.96-101
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• 1986

총29점의 국내시판 십자화과 채소종자에 대하여 검은빛썩음병(흑부병) 감염여부를 종자세척액의 농축, 선택배지에 도말방법에 의해 조사한 결과 1점의 수입 양배추종자에서 검은빛썩음병균이 검출되었다. 몇가지 종자소독방법을 검토한 결과 옥시풀($3\3%$ 과한사수소수)에 30분간 침지함으로써 자연감염종자의 검은빛썩음병균을 소독할 수 있었다. 십자화과 채소재배포장에서 발병조사 결과 검은빛썩음병이 양배추에서는 매우 중요한 병해인 것으로 나타났다.

• 정보과학회 논문지
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• 제44권10호
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• pp.1105-1111
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• 2017

영향력 최대화란 소셜 네트워크에서 최대의 영향력을 갖는 k개의 시드(seed) 노드로 이루어진 집합을 선출하는 문제이다. 이 문제를 해결한 기존 방법들이 갖는 가장 큰 문제는 시드 집합을 선출하는데 너무 많은 시간이 소요된다는 점이다. 이러한 성능 문제는 미시적, 거시적 두 가지 측면에서 발생한다. 본 논문은 미시적, 거시적 측면의 성능 문제 동시에 해결하는 효율적인 영향력 최대화 방안을 제안한다. 또한, 양질의 시드 집합을 선출하기 위한 새로운 경로 기반 커뮤니티 탐지 기법을 제안한다. 네 가지 실세계 데이터를 이용한 실험을 통해, 제안하는 방안이 미시적, 거시적 측면의 문제를 모두 해결하는 동시에 양질의 시드 집합을 선출함을 확인하였다.

• 한국식물병리학회지
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• 제14권6호
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• pp.630-635
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• 1998

Tobacco mosaic tobamovirus (TMV), cucumber mosaic cucumovirus (CMV), cucumber green mottle mosaic tobamovirus (CGMMV) and zucchini yellow mosaic potyvirus (ZYMV) from individual fruits and seeds of hot pepper and cucumber were detected by the reverse transcription-polymerase chain reaction (RT-PCR). The dilution end-points for RT-PCR in curde sap from TMV. and CMV - infected hot pepper leaves and CMV - and CGMMV-infected cucumber leaves were 10-5. However, the amount of PCR product obtained from preparation of ZYMV-infected cucumber leaf was 10-fold lower than those of CMV or CGMMV-infected cucumber leaves. In hot pepper, both TMV and CMV were detected in all parts of the fruit wall tissue, but the yields of PCR products in the fruit stalk and its surrounding tissues were higher than those of the end parts of the fruit. On the other hand, in cucumber fruit infected with CMV, CGMMV or ZYMV, the fruit wall tissue and seed located in both stalk and end parts showed higher yields of PCR products than those of intermediate parts. Of five viruses that were analysed, only TMV in hot pepper seed, and CGMMV and CMV in cucumber seed were detected in testa parts.

• 한국산업정보학회논문지
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• 제25권5호
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• pp.23-29
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• 2020

농업진흥청 국립농업과학원에서는 다양한 종류의 농작물에 대해 우량 종자 확보를 위한 생육환경 모니터링 및 수확된 종자의 분석과 같은 다양한 연구를 진행하고 있다. 본 논문에서는 농업진흥청에서 보유하고 있는 다양한 종류의 농작물 씨앗을 분석하기 위해 종자 객체 검출 방법을 제안한다. 제안된 방법은 Mask-RCNN을 이용한 전이학습을 수행하며 주어진 특정 환경 (일정한 조도, 흰색 배경)에서 촬영한 입력 영상을 종자 객체 인식을 위한 적절한 매개 변수 적합 (Tuning) 과정 및 영상 분할 작업을 진행한다. 제안된 방법으로 종자 객체 검출에 대한 실험결과로 벼 이삭 영상의 경우 82%와 단순한 볍씨 영상의 경우 97%의 정확도로 벼 낱알을 검출하였다. 향후 연구로 복잡한 상황의 종자 영상 분할을 위한 심화학습 기반의 접근법 및 검출된 종자 객체로부터 길이, 폭, 두께와 같은 정밀한 데이터 분석을 통하여 우량 종자 연구를 계획하고 있다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2001년도 춘계연합학술대회 초록
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• pp.200.2-200
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• 2001

• Mycobiology
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• 제51권4호
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• pp.195-209
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• 2023

The seed borne disease such as bakanae is difficult to control. Crop yield loss caused by bakanae depending on the regions and varieties grown, ranging from 3.0% to 95.4%. Bakanae is an important disease of rice worldwide and the pathogen was identified as Fusarium fujikuroi Nirenberg (teleomorph: Gibberella fujikuroi Sawada). Currently, four Fusaria (F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi) belonging to F. fujikuroi species complex are generally known as the pathogens of bakanae. The infection occurs through both seed and soil-borne transmission. When infection occurs during the heading stage, rice seeds become contaminated. Molecular detection of pathogens of bakanae is important because identification based on morphological and biological characters could lead to incorrect species designation and time-consuming. Seed disinfection has been studied for a long time in Korea for the management of the bakanae disease of rice. As seed disinfectants have been studied to control bakanae, resistance studies to chemicals have been also conducted. Presently biological control and resistant varieties are not widely used. The detection of this pathogen is critical for seed certification and for preventing field infections. In South Korea, bakanae is designated as a regulated pathogen. To provide highly qualified rice seeds to farms, Korea Seed & Variety Service (KSVS) has been producing and distributing certified rice seeds for producing healthy rice in fields. Therefore, the objective of the study is to summarize the recent progress in molecular identification, fungicide resistance, and the management strategy of bakanae.

• 대한의용생체공학회:의공학회지
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• 제31권6호
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• pp.427-433
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• 2010

Although tracking methods are efficient and popular for vessel segmentation, they require a seed to initiate an instance of tracking. In this paper, a new method to detect new seeds for tracking of arterial segments from CT angiography (CTA) and to construct a vascular structure is proposed. The proposed algorithm is based on shape analysis of connected components in a volume of interest around a vessel segment which was already extracted by tracking. The eigenvalues of the covariance matrix are used as the shape features for detection. The experimental results on actual clinical data showed that the results totally revealed the arterial tree not hindered by bone or veins. In visual comparison to a method which combines registration and subtraction of both pre-contrast and post-contrast CT volumes, the proposed method produced comparable results to the reference method and were confirmed of its feasibility for clinical use of reducing the cost and burden of patients.

• 식물병연구
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• 제17권1호
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• pp.52-58
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• 2011

우리나라에서 주로 재배되는 십자화과 작물(상추, 콜라비, 무, 배추, 양배추)의 종자 전염 병원균 중에서 세균성 병원균 Xanthomonns campestris pv. campestris(Xcc)와 바이러스 병원균 Lettuce Mosaic Virus(LMV)를 종자에서 동시검출하기 위한 One-step multiplex RT-PCR을 개발하였다. 각각의 병원균을 특이적으로 증폭시키는 병원균 검출용 프라이머 2종(Xcc-F/R, LMV-F/R)을 primerblast 프로그램을 이용하여 제작하였고 이들 프라이머 세트는 프라이머간 또는 병원균 cDNA간의 간섭없이 특이적으로 타겟 병원균만을 검출하였다. PCR을 이용한 병원균의 검출 최소 민감도는 1 ng이었다. 십자화과 작물중에서 유통 중인 콜라비 10품종, 상추 50품종, 무 20품종, 배추 20품종 그리고 양배추 20품종에 대한 종자 감염 병원균 검출을 위한 One-step multiplex RT-PCR 수행 결과, LMV는 전체 120품종 중에서 39품종에서 검출되었고, Xcc는 2개 품종에서 검출되었다. 그리고 50품종의 상추 종자 시료 중에 8품종의 시료에서 LMV와 Xcc가 동시 검출되었다.

• 농업과학연구
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• 제39권1호
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• pp.117-123
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• 2012

Pathogenic fungi and bacteria such as Pectobacterium atrosepticum, Clavibacter michiganensis subsp. sepedonicus, Verticillium albo-atrum, and Rhizoctonia solani were the major microorganism which causes diseases in seed potato during postharvest process. Current detection method for disease-infected seed potato relies on human inspection, which is subjective, inaccurate and labor-intensive method. In this study, a reflectance spectroscopy was used to classify sound and disease-infected seed potatoes with the spectral range from 400 to 1100 nm. Partial least square discriminant analysis (PLS-DA) with various preprocessing methods was used to investigate the feasibility of classification between sound and disease-infected seed potatoes. The classification accuracy was above 97 % for discriminating disease seed potatoes from sound ones. The results show that Vis/NIR reflectance method has good potential for non-destructive sorting for disease-infected seed potatoes.