• Plant Resources
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• v.2 no.2
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• pp.88-95
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• 1999

These experiments were conducted to evaluate the variability of seed germination and seedling growth with different levels of polyethylene glycol(PEG 6,000) solution in onion seed. Average germination percentage of seed primed in PEG solution with 1.00 and 0.75 MPa was higher than control, and that of seed primed in 1.50MPa was lower than unprimed control. Germination percentage(GP) of seed primed for 5 days was highest, and as the primed days become long, the GP was decreased. The GP of airation seed during the primed was higher than that of unairation seed, about 5% , respectively. The GP of washed seed after primed was higher than that of unwashed seed, but that of redried seed after primed was lower than that of the others. The highest GP cultivar was Chunjoogoohyung and the lowest GP cultivar was Seouldego in unredried seed after primed, but Chunjoojoonggo was highest and Jungpoonwhang was the lowest cultivar in redried seed after primed. As the PEG concentration increased, the seedling length(SL) was shortened, and seed primed for 15 days was longer than other treatments. The SL of primed seed was similar to GP. The SL of washed seed after primed was longer than that of others, but that of redried seed after primed was shortest among the others. The SL of Chunjoojoonggo and Nongwoodego was longest and Seouldego was shortest among the cultivars in unredried seed after primed, but that of Chunjoogoohyung and Chunjoojoonggo was longest and Seouldego was the shortest cultivars in redried seed after primed.

• Journal of Forest and Environmental Science
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• v.38 no.4
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• pp.256-262
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• 2022

The timing of seed harvesting is an important decision in the management of seed orchards because it affects seed quality and yield. To investigate the effect of cone harvest time on seed quality and determine the optimal harvesting time, cones were regularly collected in seven times and germination tests were performed at each harvest time in two clonal seed orchards of Chamaecyparis obtusa. As cones developed, the percentage of seed germination increased before cone moisture content began to decrease significantly. The moisture contents of cones were highest at the first collection as 68.3% and 67.3% in Jeju and Gochang seed orchards respectively. At this time, germination speed was slowest, indicating poor seed vigour. The highest germination was found at the second stage in Jeju (36.5%) and at the seventh stage in Gochang (28.6%) seed orchard. The germination speed increased as cone moisture content decreased. Additionally, changes of seed vigour differed among the developmental stages in both seed orchards. Consequently, the optimal cone harvest time of C. obtusa seed orchards in Jeju was early September when high germination percentage was obtained. In Gochang seed orchards, late October was optimal cone harvest time when the germination speed was fast and the cone moisture content decreased.

• Journal of Environmental Science International
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• v.25 no.4
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• pp.557-566
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• 2016

In nine commercial watermelon cultivars, the effects of osmotic seed priming were evaluated for seed viability and seedling growth at different germination temperatures and field conditions. Generally, primed seeds showed improved germination rate and reduced time for emergence at different germination temperatures, as compared to untreated seeds. In particular, priming effect on seed germination was significantly high at $15^{\circ}C$, which is an environment normally resulting in poor germination. Depending on the watermelon cultivar, germination rate under low temperature ($15^{\circ}C$) was improved by up to 15~66% in primed seeds, as compared to untreated seeds. However, priming effect on germination was gradually reduced as the temperature reached to its optimum level for seed germination. Seed priming tended to improve the viability, fresh weight, and dry weight of watermelon seedlings, but its effect on seedling stage was not large, as compared to untreated seeds. Primed watermelon seeds showed improved emergence rate and facilitated germination in the field, but their seedling growth after 30 days from germination was not significantly affected. Our results indicated that seed priming can greatly improve the seed germination at poor temperature conditions in the watermelon.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.3
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• pp.25-31
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• 1999

The differences in seed germination rates of Korean Native Plants were observed depending on the harvest years, the seed companies, the storage periods, the drying methods and the plant morphological types. The seed germination rates were changed significantly by the harvest years and the seed companies in all 4 species tested. The seed germination rates were decreased after 1 year storage at $4^{\circ}C$ in all 8 species tested and three of them did not germinate at all. A rapid drying method at $35^{\circ}C$ with a convection oven was not suitable for Chrysanthemum spp., but there were no significant changes of germination rates in Aster spp. The germination rates were decreased more at seeding in soil than at lab. test with Dicotyledon but no significant changes with Monocotyledon. Especially, the germination rates of fine seed on 5mm soil covering treatment were significantly decreased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.134-138
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• 2000

Most parts of alfalfa plant have been reported to contain autotoxic substances that inhibit seed germination and early seedling growth, however, the chemical(s) is not still studied much. Effect of seed leachates of 'Vernal' alfalfa (Medicago sativa L.) was evaluated for inhibition of alfalfa germination and root growth through bioassay. Alfalfa seeds were extracted in 1 L deionized water for 1 h after soaking and the leachates caused to reduce root length of alfalfa significantly as the soaking time increased. Crude seeds at 4 g L$L^{-1}$ exudated autotoxins that reduce significantly root length by 34 % compared to the control, when the seeds soaked in deionized water for 24 h. However, the extracts did not affect final germination as well as speed of germination. Extracts from ground seeds significantly reduced speed of germination (GT 50) and root length. The results indicate that release of autotoxic substances from seeds during seed imbibition was increased with increase of soaking time and seed amount, and that autotoxicity was more occurred in ground seeds than in crude seeds.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.2
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• pp.85-92
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• 2014

This text was analyzed and investigated the morphological character, production time and germination characteristic of seed that collect in Chungnam, in order to offer the basic informations for slope restoration and revegetation using Indigofera amblyantha of wild shrub. Legume was cylindrical type and length 31.5mm, width 2.5mm, and seed number of per legume was full ripe 5.5, unripe 0.9. Seed was elliptical type and length 2.4mm, width 1.7mm, and weight of 1,000 seeds was 5.9g. Because seed matured within December and next year January, optimum time of seed production was in mid of January. In the treatment $H_2SO_4$ of 15 minutes, seed germination rate was the highest as 91.0%, and seed germination time (days) was the shortest as 6.7 days. Seed germination time by temperature was the shortest from $25^{\circ}C$. Therefore, seed germination of Indigofera amblyantha was very useful in the treatment $H_2SO_4$ of 15 minutes from $25^{\circ}C$ temperature.

• Forest Science and Technology
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• v.14 no.4
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• pp.160-168
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• 2018

The seeds of Elaeocarpus serratus, a tropical underutilized fruit tree are characterized by hard seed coat and consequent poor water uptake and low germination. To improve the regeneration through seeds, various parameters such as viability of seeds, water uptake, and effect of seed mass on germination and pretreatments were performed using a completely randomized design (CRD). Tetrazolium (TZ) test was conducted using fresh, mature seeds revealed $50{\pm}2.56%$ mean viability. Seeds of different weight classes showed similar pattern of water uptake and the saturation level was achieved at 60 hrs of soaking. Seeds belong to weight class 2.6-3.5g were germinated ($12.5{\pm}1.26%$) with $175{\pm}1.75days$ (d) of mean time taken for germination (MTG). Germination capacity of seeds varied significantly among different populations and Varkala population gave $12.5{\pm}1.1%$ germination with $174.6{\pm}2.5d$ MTG. Among various seed treatments, mechanical scarification was superior in germination and significant reduction in MTG ($p{\leq}0.05$). The mechanical scarification by complete removal of seed coat resulted in $49.2{\pm}1.52%$ germination within a short period of time ($9.52{\pm}0.89d$ MTG). However, the complete removal of seed coat without damaging to embryo is a difficult task. An alternate treatment (Mechanical scarification II) by making cracks on nut faces vertically followed by soaking in distilled water for 24 hrs gave $48.4{\pm}1.73%$ germination with significantly reduced MTG ($12.14{\pm}0.56d$) over unsoaked, untreated control ($6.5{\pm}1.84%$ germination and $197.18{\pm}1.79d$ MTG; $p{\leq}0.05$). This treatment (Mechanical scarification II) is therefore recommended for E. serratus seeds as it can adopt easily and can achieve 7 fold increases in germination over control. The recorded germination through mechanical scarification is in tune with realized viability percentage of the seeds.

• Korean Journal of Plant Resources
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• v.19 no.6
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• pp.700-705
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• 2006

Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at $4{\pm}2^{\circ}C$ for 10 months or 4 years were incubated at 10, 15, 20, $25^{\circ}C$ under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at $10^{\circ}C$ were transferred to $30^{\circ}C$ to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from $10^{\circ}C\;to\;30^{\circ}C$ had a positive effect on seed germination.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.213-213
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• 2017

High quality seed of proso millet that has high germination percentage, germination speed, and uniformity demanded to increases rates of mechanization in cereal crop cultivation. In order to improve germination characteristics, proso millet seeds were treated with red light, ultrasonication and liquid smoke (LS) solution that generated from hickory wood. All treatments were performed in seed priming solution with 100mM $NH_4NO_3$ at $15^{\circ}C$ for 24hrs under aeration condition. Seeds were exposed under light intensity of 2000 lux for 15m, 30m, 60m, and 120m in priming solution. Ultrasonic treatment was performed at 60%, 80%, and 100% intensity of 21.6 KHz for 5m, 10m, and 20m in priming solution. For LS treatment seed were soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted solution with $dH_2O$ or 100mM $NH_4NO_3$ solution. The effect of seed treatment was evaluated with germination percentage (GP), mean germination time (MGT), germination index (GI), germination rate (GR), Germination uniformity (GU) and heath seed percentage (HS). Our results demonstrate that red light (15min) or ultrasonication (21.6kHz, 5min) treatment improved MGT, GI, GR, and GU comparing to untreated control. Importantly, we show that LS treatments have significant effect on the health seedling and germination characteristics. Proso millet seeds that treated with 5% LS solution for 24hrs improves HS up to 97% that similar results obtained in 100mM $NH_4NO_3$ priming for 24hrs. The combined treatment with LS solution and 100mM $NH_4NO_3$ priming were not effective in all treatments. Our results demonstrate that treating seeds with LS or 100mM $NH_4NO_3$ priming or ultrasonication improves germination characteristics. The methods described here will help advance research using this species by increasing the germination performance at which successive seed pellet process.

• Asian Journal of Turfgrass Science
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• v.2 no.1
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• pp.41-48
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• 1988

Research was conducted to obtain easy and secure methods for promoting germination of Korean lawngrass (Zoysia japonic a Steud.) seed. The effect of sodium hypochiorite (NaOCl) treatment on germination of seeds was compared with that of potassium hydroxide (KOH) treatment under conditions of duration of seed storage after harvest and temperature of treatment. Emergence of seedlings from soil and micro-flora infection rate among seed treatments were investigated. 1. The promotion of germination was much more evident in seeds treated with 4% NaOCl for 8 and 10 hours than in seeds treated with 25% or 30% KOH for 30 and 40 minutes. Longer durations of storage before seed treatment in KOH than in NaOCl were required to initiate enough germination. 2. Differences in temperature of treatment with NaOCT did not have much influence on germination of seeds and treatment temperature of 15˚C was better than that of 200˚C and 300˚C for promoting germination. But seed treatment with KOH significantly promoted germination with increasing temperature of treatment from I 5˚C to 20˚C and 30˚C. 3. GA$_3$ treatment enhanced germination in NaOCl-pretreated seeds at early stage of imbibition and in-creased about 10% germination after 10 days of imbibition in KOH-pretreated seeds. 4. NaOC1 treatments significantly decreased the rate of the infection of microflora in seed samples and enhaced emergence of seedlings from soil compared with KOH treatment. 5. NaOC1 treatment had advantage over KOH treatment with respect to ease of preparing, securing and handling in stimulating germinating of Korean lawngrass seeds.