• Journal of Microbiology and Biotechnology
• /
• 제16권9호
• /
• pp.1338-1346
• /
• 2006

Ansamitocin P-3 is a potent antitumor agent produced by A. pretiosum. A deletion mutant of A. pretiosum was constructed by deleting the asm2 gene, a putative transcriptional repressor. The deletion mutant showed a 9-fold enhanced ansamitocin P-3 productivity. The response surface method with central composite design was employed to further optimize the culture medium composition for ansamitocin P-3 production by the deletion mutant. The concentrations of four medium ingredients, dextrin, maltose, cotton seed flour, and yeast extract, which have been reported as major components for ansamitocin production, were optimized through a series of flask culture experiments. The optimum concentrations of the selected factors were found to be dextrin 6.0%; maltose 3.0%; cotton seed flour 0.53%; and yeast extract 0.45%. The maximum titer of ansamitocin P-3 was 78.3 mg/l with the optimized composition, about 15-folds higher than the unoptimized titer of 5.0 mg/l obtained with YMG medium.

• KSBB Journal
• /
• 제5권1호
• /
• pp.43-47
• /
• 1990

약난초는 내장산 이남에서만 자생하는 상록성 난으로 관상용 뿐 아니라 점질이 높은 구경은 약용(점활제)으로써 이용가치가 높은 중요한 자원식물중의 하나이다. 본 연구에서는 이와같은 약난초의 다량증식을 위하여 몇 가지 실험을 하여 다음과 같은 결과를 었다. Green pod를 이용하여 미숙종자를 개체로 유도시키는 것은 그 유도율이 낮았고 생장점을 이용, 캘러스를 유도하여 PLB를 형성시켜 개체로 유도하는 것이 용이하였다. 그러나 미숙종자의 이용도 배지조건등의 개량으로 가능하리라 사료된다. 캘러스의 형성은 Hyponex배지에 2.4-D(2mg/l)와 Kinetin(1mg/l)을 첨가하였을 때 높았다. 한편 업육조직 및 배양세포로부터 분리시킨 원형질체는 70%이상의 생존력을 나타내었고 형광현미경 관찰을 통하여 세포벽의 재생을 확인하였다. 이와 같은 실험결과는 자생란의 다량증식 뿐 아니라 잡종난 식물체를 유도하는 제반 실험에 응용될 수 있는 기초 자료가 될 수 있으리라 사료된다.

• International Journal of Internet, Broadcasting and Communication
• /
• 제13권2호
• /
• pp.82-92
• /
• 2021

Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 µL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35℃. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5-5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.

• Journal of Plant Biotechnology
• /
• 제47권4호
• /
• pp.330-336
• /
• 2020

Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

• 한국버섯학회지
• /
• 제9권4호
• /
• pp.166-169
• /
• 2011

느타리버섯 수확후 배지의 가축사료화가 가능하도록 주재료로 콘코브를 사용하여 적합한 영양원을 선발하고자 하였다. 시험에 사용된 영양원 가운데 질소함량은 채종박(7.6%)>케이폭박(6.4%)>밀기울(2.7%)순이고, 조지방함량은 밀기울(2.7%)>케이폭박(2.4%)>채종박(1.3%)>면실박(0.8%)로 나타났다. 영양원별 혼합배지의 이화학적 특성결과 C/N율은 18~20, 용적밀도 $0.27{\sim}0.28g/cm^3$, 공극률 73.1~74.8로 처리간의 차이가 없었으며, 병당 수량은 케이폭박 첨가구(케이폭박 단용, 면실박+케이폭박(1:1) 혼용, 케이폭박+밀기울(1:1) 혼용)가 153~158g으로 대조구(면실박, 150g)와 차이가 없는 것으로 나타났고, 그러나 채종유박은 초발이 소요일수도 1일 늦고 수량도 상대적으로 낮은 것으로 나타났다. 케이폭박 처리구의 수확후 배지의 사료적 가치를 분석결과 58.3%로 면실박보다 높은것으로 나타났다.

• 한국작물학회지
• /
• 제44권3호
• /
• pp.225-229
• /
• 1999

Unstable seedling stand establishment of wet direct seeding culture of rice is one of the major elements preventing the extension of its culture area. In order to develop methods of seedling stand improvement in direct seeded rice on flooded surfaces, three field experiments were conducted on silty loam soil using a cultivar 'Donjinbyeo' for three years, mainly focusing on water management after seeding and seed soaking with plant growth regulators (PGRs). Under the condition of shallow flooding after seeding, seedling stand rate increased and floating seedling rate decreased in both early and normal season seeding compared to deep flooding. With earlier draining time after seeding, there was a tendency towards preferential growth of the seminal root, increase of seedling stand and decrease of the floating seedling rate. Therefore the highest seedling numbers per unit area and the lowest floating seedling numbers were found upon drainage at 1 day after seeding (DAS), while a contrary tendency was shown upon conventional drainage at 7 DAS. Seed soaking with PGRs such as Metalaxyl or mixing of Metalaxyl with gibberellic acid (GA$_3$) significantly increased the seedling stand. In addition the effects of PGR treatment on seedling stand and the early growth of plants were greater under flooded conditions than under drained conditions after seeding, although draining of water after seeding improved the seedling establishment rate more when compared with the PGR treatment. These results suggest that draining management after seeding or maintaining of shallow flooding for a week is the most effective method to improve the seedling stand rate in wet direct seeding.

• 한국식생활문화학회지
• /
• 제4권4호
• /
• pp.417-424
• /
• 1989

한방과 식품분야에서 널리 이용되고 있는 쑥의 특성 중 항미생물 효과에 대한 연구를 하였다. 쑥씨에서 분리한 정유를 GC, GC-MS로 분석한 결과 77종의 휘발성 물질이 동정되었고 그 중에서 가장 많이 존재하는 것은 campher(14.242%), borneol(12.812%), 1.8-cineol(6.437%), terpinen-4-ol(1.185%) 등으로 전체 정유성분의 약 35%를 차지했다. 이들 4종과 Mugwort seed extract(essential oil)를 가지고 대표적인 식품 미생물인 S. cerevisiae, L. mesenteroides, L. plantarum, A. oryzae와 B. subtilis 그리고 식품오염 정도의 지표 미생물인 E. coli 등의 생육에 미치는 항미생물 효과를 측정한 결과 terpinen-4-ol이 가장 효과가 커 1000ppm 정도로 미생물의 생육을 거의 억제시켰으나 cineol, essential oil은 효과가 그다지 크지 않은 반면에 borneol과 campher는 항미생물 효과가 미약했다.

• Journal of Applied Biological Chemistry
• /
• 제64권1호
• /
• pp.97-102
• /
• 2021

본 연구에서는 들깨(Perilla frutescens)박으로부터 효소분해물을 제조하기 위한 효소를 선별하여 최적의 반응조건을 확립하였다. Alcalase와 Ceremix의 동시 처리가 들깨박의 단백질과 탄수화물의 가용화에 효과적이었으며, 효소 사용량은 들깨박 중량의 2% (w/w), pH는 7.0, 반응 시간은 2시간이 적당하였다. 최적의 반응조건에서 들깨박을 Alcalase와 Ceremix로 처리한 결과 환원당, 가용성 단백질 및 총 폴리페놀 함량이 크게 증가하였다. 유리 라디칼과 양이온 라디칼에 대한 소거활성으로 확인한 결과 들깨박 효소분해물의 항산화 활성은 대조군에 비해 우수하였다. 또한 젖산균인 Leuconostoc mesenteroides 310-12 균주를 들깨박 효소분해물에서 배양한 결과 대조군에 비해 생육과 산의 생성이 우수하였다. 결론적으로 들깨박 효소분해물은 항산화 생리활성 소재로서뿐만 아니라 유산균 배양 배지로서도 활용 가능성이 있음을 확인하였다.

• 한국산림과학회지
• /
• 제103권2호
• /
• pp.203-210
• /
• 2014

본 연구는 지구 온난화와 관련한 대응 대책 마련을 위해서 대기 온도 상승에 따른 주요 침엽수의 종자 발아 및 발아 후 유묘의 생존율 변화를 알아보고자 수행하였다. 소나무와 전나무를 대상으로 적외선등을 이용하여 대조구 보다 온난화 처리구의 대기 온도를 $3^{\circ}C$ 높게 유지하는 실외 실험적 온난화 처리를 실시하였다. 온난화 처리에 따른 대기 온도 상승으로 두 수종 모두 발아율이 높아지는 경향을 보였지만, 전나무에서만 유의적 차이가 나타났다. 소나무와 전나무 두 수종 모두 온난화 처리구가 대조구보다 평균발아일수는 유의적으로 감소하였으며, 온난화 처리구에서 높은 발아속도와 발아세를 보였다. 온도 상승과 토양 수분 감소에 따라 전나무 유묘의 고사율은 증가하였지만, 소나무는 온난화 처리에 따른 차이를 보이지 않았다. 향후, 지구 온난화에 따른 대기와 토양 온도 상승 및 토양 수분감소로 양묘과정에서 종자 발아 및 유묘 생존율의 변화가 예상되기 때문에 지구 온난화에 대응할 수 있는 양묘기술 개선이 필요할 것으로 판단된다.

• 한국조경학회지
• /
• 제18권4호
• /
• pp.73-85
• /
• 1991

Rhododendron mucronulatum for. albiflorum, native species is a shrub that has white flowers on May to June, and rare species endangered by people's rash digging or cutting. But its physiological ecological characteristics and propagation method are not being known at all. Therefore, this study was executed to utilize this species as the planting material for landscaping by analysing its habitat environment and growth form, and also experimenting its seed and vegetative propagation, and it field culture and utilization. The results are as follows; 1. The elevation, gradient and direction of this species were 295-1,350m, 10-36$^{\circ}$, northwest respectively. It was found that the species is shade-liking plant that grows under forest cover of average 51.33%. 2. The soil pH and water content of its habitat were 5.4, 25.41% respectively. The organic matter content was 6.29% that was higher than 3.2%, the average organic matter content of forest soil in Korea. 3. Representative plant community within which this species was living was Quercus mongolica community, and its main neighboring species were Lindea obtusiloba, Fraxinus sieboldiana, Rhus trichocarpa, Rhododendron Schlippenbachii, Rododendron mucronulatum. 4. The leaf length and width of this species were 39.18mm, 12.60mm respectively. This result showed that generally its leaf size was larger than that of R. micranthum, R. yedoense var. poukhanense and R. mucronulatum var. ciliatum and smaller than that of R. mucronulatum and R. schlippenbachii. 5. The whole size of its pollen was, as 59${\times}$61$\mu\textrm{m}$, the largest of plants of Rhododendron family including R. mucronulatum and R. mucronulatum var. ciliatum. 6. The result of seed germination experiment at intervals of 5$^{\circ}C$ from 15$^{\circ}C$ to 30$^{\circ}C$ presented the highest germination rate of 94.7% at 20$^{\circ}C$ numerically, but high percent germination at all temperature levels without significant difference. And the seed of this plant proved to be sun-liking seed at requiring dormancy in germination. 7. Through seed germination experiment by treatment of growth regulators such as GA. Thiourea and Kinetin under dark condition, it was found that the effect of GA treatment on germination increase and acceleration was the highest. 8. In greenwood cutting, rooted rate by treatment of various concentration of IBA and NAA on clay and vermiculite bed was not wholly high, but 100ppm plots of both IBA and NAA of clay bed showed relatively good rooted rate. 9. As result of field culture experiment for finding out optimum growth temperature and light intensity, growth conditions such as height, number of leaves, fresh weight and chlorophyll contents were the best at night/day temperature of 20/25$^{\circ}C$ and under 1/2sun. Also, the photosynthetic rate was the highest at 25$^{\circ}C$. Accordingly, it was found that optimum temperature and light intensity for growth of this plant are 25$^{\circ}C$ (day temperature), 50% of natural light respectively.