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http://dx.doi.org/10.3839/jabc.2021.015

Preparation of enzymatic hydrolysate from defatted perilla seed residue and its application to Leuconostoc mesenteroides cultivation  

Shin, Yeung Sub (Department of Chemical Engineering, Chungwoon University)
Lee, Tae Jung (Department of Chemical Engineering, Chungwoon University)
In, Man-Jin (Department of Chemical Engineering, Chungwoon University)
Kim, Dong Chung (Department of Chemical Engineering, Chungwoon University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.1, 2021 , pp. 97-102 More about this Journal
Abstract
In this study, enzymes were screened for hydrolysis of defatted perilla seed residue (DPSR) and optimal conditions for enzymatic treatment were determined to produce the hydrolysate of DPSR. Also its antioxidant activity and utilization as a culture medium were examined. The combined treatment of Alcalase and Ceremix is most effective for solubilization of protein and carbohydrate in DPSR. The optimal dosage, pH, and reaction time for enzymatic treatment were found to be 2.0% (w/w), 7.0, and 2 h, respectively. Treatment with optimal conditions of enzymes dramatically increased reducing sugar, soluble protein, and total phenolic content. The hydrolysate of DPSR possessed better scavenging activity against cation and free radicals than enzyme-untreated extract. When Leuconostoc mesenteroides 310-12 was cultured in the hydrolysate of DPSR, cell population rapidly increased compared to enzyme-untreated extract, and titratable acidity increased in proportion to the bacterial growth. In conclusion, these results imply that the hydrolysate of DPSR could be utilized as a bacteria culture medium as well as a physiologically active material with antioxidant activity.
Keywords
Defatted perilla seed residue; Enzymatic hydrolysis; Leuconostoc mesenteroides; Microbial medium;
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Times Cited By KSCI : 3  (Citation Analysis)
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