• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.3
• /
• pp.302-307
• /
• 1996

Seed coat anthocyanin can be purified by soaking 3 times in methanol solution supplemented with one percent of HCl. Anthocyanin content was very wide range in collected lines and average anthocyanin content of black seed coat lines was 15.07 permillage, but that of white mottled on brown seed coat lines was 0.31 permillage. In black seed coat lines green seed embryo type has more anthocyanin in amount compare to yellow seed embryo. Anthocyanin accumulation was promoted in late maturing lines compare to early maturing lines. Positive correlations were observed among 100 seed weight, days to flowering, days to growing and anthocyanin content, but negative correlation between days from flowering to maturity and anthocyanin content. Collected black seed coat lines were divided into two maturity groups. Group VI which has longer than group V in days to maturity accumulated more anthocyanin compare to group V. When the seeding date was May 15, highest anthocyanin content was observed.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.6
• /
• pp.941-945
• /
• 2004

To increase utilities as functional materials, nine peanut varieties were evaluated on antioxidant activity and total phenol content. Peanut varieties were individually divided into three parts such as part without seed coat, seed coat, and seed. Each of the 80% ethanol-extracted solutions from the parts was used for the assay. Inhibitory activities on low density lipoprotein (LDL) oxidation by the part without seed coat, seed coat, and seeds from 9 varieties were respectively 85.3∼91.0%, 77.4∼85.8%, -1.7∼46.1%, and those on linoleic acid oxidation were 94.0∼94.9%, 94.2∼95.0%, 69.4∼80.2%, respectively. Seed coats, parts without seed coat, and seeds from 9 peanut varieties showed scavenging activities as 88.3∼98.7%, 16.7∼56.3%, -28.0∼20.0% on superoxide anion radical and as 89.9∼90.8%, 6.0∼12.0%, 0.6∼2.1% on 1,1-diphenylpicrylhydrazyl (DPPH) radical, respectively. Total phenol contents of seed coat, seed, and part without seed coat were 12.7∼21.0 mg%, 2.46∼4.24 mg%, 0.94∼1.16 mg% as tannic acid equivalent and those of Daekwang, Daesin, Bowon, Saedl, Kipung among the peanut varieties were comparatively high. In conclusion, Daekwang, Daesin, Kipung, and Saedl among the peanut varieties showed comparatively effective antioxidant activities.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.1
• /
• pp.55-62
• /
• 1994

The germplasms of naked barley evaluated for starch content were 137 crossing blacks and progeny lines obtained from the breeding program of the Wheat and Barley Research Institute, 540 Tabdong Kwonsungu Suwon, in the 1988/1989 season. Starch content was distributed between 49.15 and 62.50% with a mean of 57.20%. It was high in the bred lines and the Uz type lines than the imported, uz type or lax type ones. The range observed for seed coat weight was 1.12-3.02mg and the mean seed coat weight was 1.88mg. The seed coat weight ratio to kernel weight was measured between 4.0 and 8.7% with a mean of 6.5%. The varieties differ greatly in the characteristics depending on the plant type and origin of germplasms. The variation observed with protein content ranged from 9.60 to. 15.90% while the average was 12.86%. Starch content was negative relationships with kernel weight, seed coat weight, seed coat weight ratio., kernel width, kernel thickness and protein content, and starch content was higher in smaller kernels within a variety. The weight and weight ratio. of seed coat were positively correlated with the width, thick-ness of kernels, whilst they were negatively correlated with starch content. The heritability of starch content was 30.0%.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2021.04a
• /
• pp.15-15
• /
• 2021

Seed coat color and seed weight are among the key agronomical traits that determine the nutritional quality of soybean seeds. This study aimed to evaluate the contents of total protein, total oil and five prominent fatty acids in seeds of 49 soybean varieties recently cultivated in Korea, and assess the influences of seed coat color and seed weight on each. Total protein and total oil contents were in the ranges of 36.28-44.19% and 13.45-19.20%, respectively. Likewise, individual fatty acid contents were in the ranges of 9.90-12.55, 2.45-4.00, 14.97-38.74, 43.22-60.26, and 5.37-12.33% for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. Our results found significant variations of protein, oil and fatty acid contents between the soybean varieties. Moreover, both seed coat color and seed weight significantly affected total oil and fatty acid contents. Total protein content, however, was not significantly affected by any factor. Among colored soybeans, pale-yellow soybeans were characterized by a high level of oleic acid (30.70%) and low levels of stearic (2.72%), linoleic (49.30%) and linolenic (6.44%) acids, each being significantly different from the rest of colored soybeans (p < 0.05). On the other hand, small soybeans were characterized by high levels of all individual fatty acids except oleic acid. The level of oleic acid was significantly high in large seeds. Cluster analysis grouped the soybeans into two classes with notable content differences. Principal component analysis also revealed fatty acids as the prime factors for the variability observed among the soybean varieties. As expected, total oil and total protein contents showed a negative association with each other (r = -0.714, p < 0.0001). Besides, oleic acid and linoleic acid showed a tradeoff relationship (r = -0.936, p < 0.0001) which was reflected with respect to both seed coat color and seed weight. In general, the results of this study shade light on the significance of seed coat color and seed weight to distinguish soybeans in terms of protein, oil and fatty acid contents. Moreover, the soybean varieties with distinct characteristics and nutritional contents identified in this study could be important genetic resources for consumption and cultivar development.

• Journal of Ginseng Research
• /
• v.16 no.1
• /
• pp.37-43
• /
• 1992

Structural changes of the endosperm of Panax ginseng C.A. Meyer from fertilization to germination were investigated by light microscope. The endosperm of the ginseng seed is cellular type. Since endosperm cells adjacent embryo continuously breakdown and disappear with the elongation of embryo, the real of endosperm is gradually decreased. As the anatropous ovules of immature seed with green seed coat developes more and more, ovary cells adjacent ovary cavity become abundant by the periclinal division, their size is decreased, hypotrophy of cell wall discern, and they are gradually differentiated in seed coat. Though embryo responds strongly to basic dye at the stage of completion of endosperm formation, tissue of endosperm responds to acidic dye positively Cell wall of embryo and endosperm are composed of primary cell wall not lignified. Endosperm cells adjacent embryo begin to breakdown in the endosperm tissue of indehiscent seed before the beginning of the after-ripening. Dehiscent seed of which seed coat is opened through after-ripening represent the form as a seedling in the result of embryo developments with the formation of organs; radicle, cotyledon, plumule. Umbilifom layer represents strong positive response to the toluidine blue and the basic function. Umbiliform layer that endosperm cells breakdown and disappear is observed clearly at the periphery of the embryo cotylemon, while slightly at the periphery of the radicle.

• Current Optics and Photonics
• /
• v.3 no.6
• /
• pp.571-576
• /
• 2019

The morphological changes of anthracnose (fungus) -infected tomato seeds have been studied to identify the infection and characterize its effect. Full-field optical coherence tomography (FF-OCT) has been utilized as a nondestructive but efficient modality for visualizing the effects of fungal infection. The cross-sectional images extracted from a stack of en face FF-OCT images showed significant changes with infection in the seed structure. First of all, the seed coat disappeared with the infection. The thickness of the seed coat of a healthy seed was measured as 28.2 ㎛, with a standard deviation of 1.2 ㎛. However, for infected seeds the gap between surface and endosperm was not appreciably observed. In addition, the measurements confirmed that the dryness of seeds did not affect the internal seed structure. The reconstructed three-dimensional (3D) image revealed that the permeability of the seed coat, which plays the vital role of protecting the seed, is also affected by the infection. These results suggest that FF-OCT has good potential for the identification of fungus-infected tomato seeds, and for many other tasks in agriculture.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.52-52
• /
• 2019

Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.

• Korean Journal of Plant Taxonomy
• /
• v.38 no.1
• /
• pp.1-16
• /
• 2008

Anatomical features of seed coat were examined on Trollius, Calathodes, and Caltha of Ranunculaceae to evaluate the taxonomic circumscription of Megaleranthis saniculifolia, which is monotypic and endemic in Korea. Megaleranthis saniculifolia showed the exotestal type of seed coat exhibiting a well-developed palisade structure in exotesta and its external surface of exotesta cells was concave. On the other hand, the shape of exotesta cells in Caltha was cuboidal and the outer surface was smooth. The exotesta of Calathodes seeds was formed of the palisade structure like M. saniculifolia, but the outer surface of exotesta cells was smooth. The palisade structure was much better developed in the exotesta of Calathodes as well as Megaleranthis seeds than in the exotesta of Trollius seeds. The outer surface of exotesta cells in Trollius was either convex or concave according to the species examined. Since the genera of the subtribe Calthinae of the family display differences in seed sculpturing and the anaotmy of seed coat, these characteristics are useful to access taxonomic relationships among them. The morphological and anatomical features of seed coat suggest that Megaleranthis be possibly allied with Trollius rather than Caltha or Calathodes. Concave surface of seed coat cells and well-developed palisade structure of exotesta are shared by M. saniculifolia and some species of Trollius in common.

• Food Science and Preservation
• /
• v.30 no.5
• /
• pp.896-904
• /
• 2023

The texturization characteristics of textured vegetable protein (TVP) were investigated based on the extent of soybean decoating during the pretreatment of defatted soybean flour used for TVP. The raw materials for TVP consisted of 50% defatted soybean flour, 30% gluten, and 20% corn starch. The weight ratios of soybean seed coat to soybean flour were 9%, 6%, 3%, and zero. Extrusion was performed using an extruder equipped with a cooling die, maintaining a barrel temperature of 190℃ and screw speed of 250 rpm, Water was injected at a rate of 9 rpm using a metering pump. Regarding the textures of the extruded TVPs produced from defatted soybean flour, an increase in the soybean seed coat content led to a decrease in the apparent fibrous structural layer and an increase in hardness. However, there were no significant changes in elasticity and cohesion. Moreover, as the soybean seed coat content increased, the pH of TVPs decreased. A higher soybean seed coat content also tended to lower the moisture content, increasing water absorption, solids elution, and turbidity. These results suggest that an increased seed coat content reduces the proportion of protein, and the fibers present in the seed coats prevent texturization.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 1988.07a
• /
• pp.58-59
• /
• 1988