• Applied Microscopy
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• v.36 no.3
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• pp.217-226
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• 2006

Image processing by ultra high voltage electron microscopy (UHVEM) and tomography has offered major contributions to research in the field of cellular ultrastructure. Furthermore, such advancements also have enabled the improved analysis of three-dimensional cellular structures in botany. In the present study. using UHVEM and tomography, we attempted to reconstruct the three-dimensional images of plastid inclusions that probably differentiate during photosynthesis. The foliar tissues were studied Primarily with the TEM and further examined with UHVEM. The spatial relationship between tubular elements and the thylakoidal membrane and/or starch grains within plastids mainly have been investigated in CAM-performing Sedum as well as in $C_4$ Salsola species. The inclusion bodies were found to occur only in early development in the former, while they were found only in mesophyll cells in the latter. The specimens were tilted every two degrees to obtain two-dimensional images with UHVEM and subsequently comparison has been made between the two types. Digital image processing was performed on the elements of the inclusion body using tilting, tomography, and IMOD program to generate and reconstruct three-dimensional images on the cellular level. In Sedum plastids, the inclusion bodies consisted of tubular elements exhibiting about 20 nm distance between elements. However, in Salsola, plastid inclusion bodies demonstrated quite different element structure, displaying pattern, and origin relative to those of the Sedum. The inclusion bodies had an integrative relationship with the starch grains in both species.

• Journal of agriculture & life science
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• v.46 no.2
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• pp.35-42
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• 2012

This study was conducted to investigate growth responses at 45 days after cutting in six Sedum cultivars (S. reflexum 'Blue Spruce', S. rupestre 'Angelina', S. sexangulare, S. sexangulare var. elatum, S. spurium 'Atropurpureus', S. takesimense) using vegetation mats (individual coir tape and jute treated with different thicknesses and soil compositions) to broaden usefulness of evergreen Sedum cultivars. All the treatments including both single and interaction between treatments showed significant effects (p<0.001) on the number of branches, plant height, and shoot fresh weight except the number of branches treated with different mat types. No. of branches was showed two times in compost than in sand regardless of cultivars and mat types with the exception of S. takesimense showing no added branches in all treatments. Each plant height of all the cultivars except 'Angelina' showed the highest value under the conditions treated with 5 mm thick coir tape using compost. The fresh weight of 'Blue Spruce' treated with 5 mm thick coir tape using compost was 13.19 g of which value was the highest in all treatments. The better growth response in all Sedum cultivars was shown in the treatment of 5 mm thick coir tape than with jute net treatment.

• Natural Product Sciences
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• v.17 no.4
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• pp.279-284
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• 2011

Phytochemical studies on the whole extract of Sedum hybridum L., a Mongolian medicinal plant, has been undertaken to isolate active principles responsible for its anti-oxidative and antibacterial activities. Eighteen known compounds, i.e. (1) quercetin, (2) kaempferol, (3) herbacetin-8-O-${\beta}$-D-xylopyranoside, (4) myricetin, (5) gossypetin-8-O-${\beta}$-D-xylopyranoside, (6) gallic acid, (7) 2,4,6-tri-O-galloyl-D-glucopyranose, (8) 6-O-galloylarbutin, (9) myricetin-3-O-${\alpha}$-L-arabinofuranoside, (10) quercetin-3-O-${\alpha}$-L-arabinofuranoside, (11) caffeic acid, (12) ethylgallate, (13) (-) epigallocatechin-3-O-gallate, (14) palmitic acid, (15) stearic acid, (16) stearic acid ethyl ether, (17) ${\beta}$-sitosterol and (18) ${\beta}$-sitosteryl-O-${\beta}$-D-glucopyranose have been isolated and their molecular structures identified by spectroscopic analysis. Thirteen substances including seven flavonol components (1, 2, 3, 4, 5, 9 and 10), five gallic acid derivatives (6, 7, 8, 12 and 13) and caffeic acid (11) exhibited significant, dose-dependent, DPPH radical scavenging activity. Galloyl esters 12 and 13 were revealed to be main active principles for the antibacterial property of the extract of Sedum hybridum L.

• Journal of the Korean Applied Science and Technology
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• v.21 no.3
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• pp.204-213
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• 2004

Extraction of Sedum sarmentosum Bunge by super critical carbon dioxide was operated under $40-50^{\circ}C$and 200-250 atm, thus, yield of extraction was very low as 4 wt%. Rats were administrated with the extract orally once a day for succesive 6 days, followed by treatment with carbon tetrachloride ($CCl_4$) on the sixth day. The activities of aminotransferase, alkaline phosphatase, ${\gamma}$-glutamyl transpeptidase, lactate dehydrogenase and contents of triglyceride, total cholesterol in the extract-pretreated rats were decreased compared to the $CCl_4$controled rats, whereas content of HDL-cholesterol was increased. Especially content of hepatic malondialdehyde (MDA) and atherogenic index (AI) were decreased and HTR was increased in the extract-pretreated rats, and reduced peroxidative liver damage in the $CCl_4$-induced hepatotoxicity rats. In addition, activities of hepatic superoxide dismutase, catalase, glutathione peroxidase in the extract-pretreated rats were significantly decreased compared to the $CCl_4$ controled rats, but the content of glutathione was significantly increased. These results suggest that extract of Sedum sarmentosum Bunge has hepatoprotective effect in the $CCl_4$-intoxicated rats.

• Preventive Nutrition and Food Science
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• v.11 no.1
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• pp.6-9
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• 2006

This study was conducted to evaluate the antioxidant activity of each fraction from Sedum sarmentosum. Antioxidant activity of each fraction was measured using the DPPH radical assay, the ferric thiocyanate (FTC) method, and thiobarbituric acid (TBA) method. The antioxidant activities were then compared with that of BHT(synthetic antioxidant). The ethyl acetate and butanol fractions were found to have significant DPPH radical scavenging activity, with scavenging potencies showing 90.61 % and 87.02%, respectively. Total phenolic compound contents, determined according to the Folin-Denis method, were found to be in the order of ethyl acetate>butanol>ethanol>chloroform>aqueous fraction. From the results, we have been able to establish a positive correlation between the antioxidant activity and the total phenolic compound content of the sample. The antioxidant activity in a linoleic acid system was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. The ethyl acetate fraction had the highest antioxidant activity among the tested fractions. On the basis of these results, the ethyl acetate fraction provided equivalent or higher antioxidant activity as compared to BHT. These results suggest that Sedum sarmentosum is a potentially useful antioxidant for foods, cosmetics, and medicine.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.285-289
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• 1997

Leaf and stem explants of Sedum erythrostichum Miq. were cultured on MS medium supplemented with various combinations of growth regulators. After two weeks of culture, 100% of the leaf explants formed calli on medium containing 2.0 mg/L 2, 4-D and 1.0 mg/L BA. Callus proliferated when subcultured on medium containing 2.0 mg/L 2, 4-D and 1.0 mg/L BA. Numerous adventitious buds were regenerated from callus cultured on the medium containing 2.0 mg/L NAA and 1.0 mg/L BA. Root formation from shoot was occurred on the MS basal medium containing 1.0 mg/L IAA.

• Preventive Nutrition and Food Science
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• v.17 no.2
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• pp.152-157
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• 2012

This study was performed to increase the availability of Sedum sarmentosum (Dolnamul) and to improve the nutraceutical value of rice cakes (sulgitteok). The contents of crude protein, mineral, dietary fiber, water holding capacity, and hardness significantly and directly increased with lyophilized sedum powder (SP). Pore ratio and expansion rate decreased in samples containing more than 10% SP compared to the control. In a sensory evaluation, a positive correlation was detected between overall acceptability and taste ($R^2$=0.99, p<0.01), and color ($R^2$=0.72, p<0.05). Total polyphenol contents of the SP-treated groups were significantly elevated, accompanied by an increase in radical scavenging ability estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Replacing 10% of the rice powder with SP efficiently improved the antioxidant and nutritional values of sulgitteok as well as its the sensory quality.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.2
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• pp.102-112
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• 2003

This study was carried out to research and develop a shallow green rooftop system which would require low maintenance and therefore could be used for existing rooftops. To achieve these goals, the conceptual model was induced by past studies and the experimental systems were deduced from the conceptual model. On the growth of Sedum sarmentosum grown in these rooftop systems, the effects of artificial substrate type, soil depth, and drainage type were investigated from 3 April to 11 October 2002. Artificial substrates were an alone type and a blending type. The alone type was an artificial substrate formulated by blending crushed porous glass with bark(v/v, 6:4). The blending type was formulated by blending the alone type with loam(v/v, 1:1). Soil depths were 5cm, loom, and 15cm. Drainage types were a reservoir-drainage type and a drainage type. The reservoir-drainage type could keep water and drain excessive water at the same time. The drainage type could drain excessive water but could not keep water. Covering area, total fresh and dry weight, visual quality, and water content per 1g dry matter were measured. All the variables were analyzed by correlation analysis and factor analysis. The results of the study are summarized as follows. The growth increment was higher in the blending type than in the alone type, the highest in loom soil depth and higher in the reservoir-drainage type than in the drainage type. The growth quality was higher in the blending type than in the alone type, the highest in l0cm soil depth, and higher in the drainage type than in the reservoir-drainage type. In consideration of the permissible load on the existing rooftops and the effects of the treatments on the growth increment and quality, the system should adopt the blending type in artificial substrate types, 5~10cm in soil depths, and the drainage type in drainage types. This system will be well-suited to the growth of Sedum sarmentosum, and when the artificial substrate was in field capacity, the weight will be 75~115kg/$m^2$.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.4
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• pp.19-24
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• 2001

14 kinds of perennial flower sods, including 10 Korean native wild perennial flowers such as Lychnis cognata, Achillea sibirica, Sedum spectabile, Sedum takesimense etc. were made with basal sheets of nonwoven fabric or jute fabric. Growth of plants planted on bark was better than that of planted on soil of open area. The plants planted on bark also showed difference in growth rate by the kinds of used basal sheets. In general, the growth of plants was good when nonwoven fabric was used as basal sheet instead of jute fabric. When jute fabric was used as basal sheet, It was severely decayed during growth period resulting in great decreasing of root sticking capacity to basal sheet. It was shown that the procumbent plants such as Tymus quinquecostatus, Ixeris stolonifera, Sedum oryzifolium, Sedum polystichoides having lower plant height and better ground coverage ability compared with upright plants such as Sedum spectabile and Aster glehni were more suitable for floral sod production.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.515-516
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• 2006

This experiment was carried out to find out proper culture soil for the flowerpot cultivation of Sedum kamtschaticum. Peatmoss was used for the culture soil. pH of the culture soils were adjusted to 4.5, 5.0, 5.5 and 6.0 using calcium hydroxide. Young plants of Sedum kamtschaticum were planted in the pots of 10cm in diameter. The experiment was conducted by the completely randomized design with 3 replications. Growth characteristics were investigated at intervals of 30 days after planting. As the pH of culture soil is lower, growth of Sedum kamtschaticum showed longer plant height and more number of leaves and branches. pH 4.5 to 5.0 appeared to be optimum range as soil reaction of Sedum kamtschaticum cultivation.