• Journal of the Korean Applied Science and Technology
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• v.18 no.4
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• pp.306-315
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• 2001

Our study is aimed at proposal of systematic verification method of molecular structure using measuring method of selective ionic determination and spectrometry on 34 kinds of surfactants such as sodium dodecyl sulfate(SDS) which are most widely used today. In the IR spectrum, unsaturated fatty acids reveal themselves by HC= at $3000{\sim}3020cm^{-1}$, and intensity of $720cm^{-1}$ depends on carbon length of alkyl group. Also ethylene oxide(EO) adducts exhibit weak characteristic bands by $-CH_{2}-CH_{2}-O$ at 1350, 1100 and $950cm^{-1}$. Isethionate can be distinguished from diester succinate by intensity ratio of 1740 and $1200cm^{-1}$ spectrums, the ratio of latter is close to 1 due to 2 carboxylate radical in diester succinate. Quaternary ammonium salts exhibit characteristic band of $C_{4}N^{+}$ at $1000-900㎝^{-1}$. In the case of dialkyl dimethyl ammonium salts in quaternary ammonium surfactants, the spectrum of $3000cm^{-1}$ by $N-CH_{3}$ collapses to a very weak band at $3020cm^{-1}$. In ammonium heterocyclic derivatives, pyridinium salts show characteristic bands at 1640 and $1460cm^{-1}$, while imidazolinium salts exhibit characteristic band at $1620-1610cm^{-1}$. In the characteristic spectrum at $1080-1050cm^{-1}$ on OH radicals of the alkyl esters, primary alcohol appears as weak band and the 2 bands show in almost same intensity when primary and secondary alcohols exist together in one molecule. Also, alkyl ester of polyhydric alcohols appears as various broad band.

• Structural Monitoring and Maintenance
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• v.5 no.4
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• pp.463-488
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• 2018

In the last decades, research efforts have been spent to investigate the effect of prestressing on the dynamic behaviour of prestressed concrete (PSC) beams. Whereas no agreement has been reached among the achievements obtained by different Researchers and among the theoretical and the experimental results for simply supported beams, very few researches have addressed this problem in continuous PSC beams. This topic is, indeed, worthy of consideration bearing in mind that many relevant bridges and viaducts in the road and railway networks have been designed and constructed with this structural scheme. In this paper the attention is, thus, focused on the dynamic features of continuous PSC bridges taking into account the effect of prestressing. This latter, in fact, contributes to the modification of the distribution of the bending stress along the beam, also by means of the secondary moments, and influences the flexural stiffness of the beam itself. The dynamic properties of a continuous, two spans bridge connected by a nonlinear spring have been extracted by solving an eigenvalue problem in different linearized configurations corresponding to different values of the prestress force. The stiffness of the nonlinear spring has been calculated considering the mechanical behaviour of the PSC beam in the uncracked and in the cracked stage. The application of the proposed methodology to several case studies indicates that the shift from the uncracked to the cracked stage due to an excessive prestress loss is clearly detectable looking at the variation of the dynamic properties of the beam. In service conditions, this shift happens for low values of the prestress losses (up to 20%) for structure with a high value of the ratio between the permanent load and the total load, as happens for instance in long span, continuous box bridges. In such conditions, the detection of the dynamic properties can provide meaningful information regarding the structural state of the PSC beam.

• Geomechanics and Engineering
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• v.32 no.4
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• pp.375-385
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• 2023

There are many joint fissures distributed in the engineering rock mass. In the process of geological history, the underground rock mass undergoes strong geological processes, and undergoes complex geological processes such as fracture breeding, expansion, recementation, and re-expansion. In this paper, the damage-stick-slip process (DSSP), an analysis model used for rock mass failure slip, was established to examine the master control and time-dependent mechanical properties of the new and primary fractures of a multi-fractured rock mass under the action of stress loading. The experimental system for the recemented multi-fractured rock mass was developed to validate the above theory. First, a rock mass failure test was conducted. Then, the failure stress state was kept constant, and the fractured rock mass was grouted and cemented. A secondary loading was applied until the grouted mass reached the intended strength to investigate the bearing capacity of the recemented multi-fractured rock mass, and an acoustic emission (AE) system was used to monitor AE events and the update of damage energy. The results show that the initial fracture angle and direction had a significant effect on the re-failure process of the cement rock mass; Compared with the monitoring results of the acoustic emission (AE) measurements, the master control surface, key blocks and other control factors in the multi-fractured rock mass were obtained; The triangular shaped block in rock mass plays an important role in the stress and displacement change of multi-fracture rock mass and the long fissure and the fractures with close fracture tip are easier to activate, and the position where the longer fractures intersect with the smaller fractures is easier to generate new fractures. The results are of great significance to a multi-block structure, which affects the safety of underground coal mining.

• Journal of the Korean School Mathematics Society
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• v.26 no.2
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• pp.159-184
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• 2023

In this study, after defining motivating efficacy operationally, we developed a draft of the Motivating Efficacy Scale for Mathematics Teachers (MESMT), a measure of mathematics teachers' motivating efficacy, through the literature review and an expert Delphi survey, and conducted the exploratory factor analysis using online survey responses from 347 elementary and secondary mathematics teachers across the country to explore the factor structure of the measure and to test its validity and reliability. The exploratory factor analysis resulted in the deletion of 17 items from the initial 42 items developed through the literature review and expert Delphi survey and the identification of four factors (Providing successful experiences, Eliciting attention and engagement, Creating mathematics case-based relevance, and Providing extrinsic rewards), resulting in a final MESMT of 25 items. The MESMT developed in this study is a valid and reliable measure of mathematics teachers' motivating efficacy, and is expected to serve as a starting point for many subsequent studies to understand mathematics teachers' motivating efficacy and improve mathematics teachers' ability to motivate students' mathematics learning.

• Journal of Korean Society of Forest Science
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• v.79 no.1
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• pp.33-41
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• 1990

The purpose of this study is to investigate the developmental process of plant community during the secondary succession through the analysis of floristic composition, life form etcectra in burned site, and the structure of forest stand before forest fire occurrence was estimated by the method of anatomical wood identification for dead trees in burned site, Cheisuk-bong in Mt. Chiri of Korea. Plant community before forest fire occurrence was estimated the high mountain conifer stand of the last successional stage to consist of Pinus koraiensis(75%), Abies koreana(15%) and Picea jezoensis(10%), and Betula costata was survived only one species after forest fire occurrence. The total number of plant species in the investigated area was 19 families and 23 species that was identified. The dominate species in burned site were Festuca ovina and Arrhenatherum elatius, Carex humilis, Ligularia fischeri etcectra in the investigated area flora. The life form of plant in burned site was the erect from 47.8%, the tufted from 30.4%, the branched and the prostrate from was both 8.7%, It was assumed that dominant species in the burned sites were Gramineae (Festuca ovina, Arrhenatherum elatius), Carex humilis ${\rightarrow}$Sorbus commixta, Rhododendron schrippenbachii, Sambucus williamsii var. coreana, Tripterygium regelii ${\rightarrow}$Acer pseudo-sieboldianum var. koreanum, Betula costata, Quercus mongolica${\rightarrow}$Pinaceae forest(Pinus koraiensis, Abies koreana, Picea jezoznsis).

• Economic and Environmental Geology
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• v.45 no.3
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• pp.295-306
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• 2012

In this study, the Singal fault zone in the Gyeonggi massif is identified in the Kiheung area. Geotechnical investigations were carried out to locate and characterize of the Singal fault zone in the Kiheung reservoir area. The N-S striking Shingal fault is known to be a Riedel-type strike-slip fault within the Choogaryung rift. Along the fault zone, 62 bore holes were drilled and electrical resistivity survey of about 11km, and vibroseis seismic refraction and reflection survey of about 500m were done. From the result of investigations, it is found that the fault zone, consisting mainly of gouge and breccia, has maximum width of 300 meters with anastomosing geometry of secondary fractures developed subparallel to the fault zone. We interpret these geometric features to be the result of structural development of flower-structure type at the restraining band of strike-slip fault. However, there are uncertainties of this interpretation because there are virtually no outcrops in the area. Further investigation to understand geometric features and linkage style of the fault zone.

• Parasites, Hosts and Diseases
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• v.58 no.1
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• pp.73-79
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• 2020

Echinostoma revolutum is a zoonotic food-borne intestinal trematode that can cause intestinal bleeding, enteritis, and diarrhea in human and birds. To identify a suspected E. revolutum trematode from a red-crowned crane (Grus japonensis) and to reveal the genetic characteristics of its mitochondrial (mt) genome, the internal transcribed spacer (ITS) and complete mt genome sequence of this trematode were amplified. The results identified the trematode as E. revolutum. Its entire mt genome sequence was 15,714 bp in length, including 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and one non-coding region (NCR), with 61.73% A+T base content and a significant AT preference. The length of the 22 tRNA genes ranged from 59 bp to 70 bp, and their secondary structure showed the typical cloverleaf and D-loop structure. The length of the large subunit of rRNA (rrnL) and the small subunit of rRNA (rrnS) gene was 1,011 bp and 742 bp, respectively. Phylogenetic trees showed that E. revolutum and E. miyagawai clustered together, belonging to Echinostomatidae with Hypoderaeum conoideum. This study may enrich the mitochondrial gene database of Echinostoma trematodes and provide valuable data for studying the molecular identification and phylogeny of some digenean trematodes.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.318-327
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• 2007

This study presents the molecular phylogenetic analysis of Korean Aurelia aurita Linnaeus collected from Yeosu in the southern waters of Korea using nuclear ITS1 region and mitochondrial COI gene sequences. The use of oligonucleotide primers F5 (forward) and R5 (reverse) targeted to ITS1 and LCO1490 (forward) and HCO2198 (reverse) targeted to COI amplified 267 bp and 643 bp fragments, respectively. The shortest genetic distance towards the ITS1 region is estimated at 0.023 when comparing Korean A. aurita to Aurelia sp. collected from California, USA. In particular, Korean and American/Swedish A. aurita were located far away in terms of genetic distance, ranging from 0.393 to 0.395. On the other hand, the genetic distance between Korean and English/Turkish/Swedish/American A. aurita regarding the mitochondrial DNA COI gene ranged from 0.201 to 0.205. However, a sister-ship with Korean and American A. aurita showed an extremely high bootstrap value (100%). The predicted secondary RNA structure of the mitochondrial DNA COI gene showed many different folding structures with a similar energy between Korean and American A. aurita. These results suggest that ITS1 and the mitochondrial DNA COI gene could be used as genetic markers for identification of the biogeographic populations.

• Journal of Microbiology and Biotechnology
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• v.21 no.8
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• pp.808-817
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• 2011

Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy single-step ion-exchange chromatographic method for purification of ThCBHI and its initial biophysical and biochemical characterization. The ThCBHI produced by induction with microcrystalline cellulose under submerged fermentation was purified on DEAE-Sephadex A-50 media and its identity was confirmed by mass spectrometry. The ThCBHI biochemical characterization showed that the protein has a molecular mass of 66 kDa and pI of 5.23. As confirmed by smallangle X-ray scattering (SAXS), both full-length ThCBHI and its catalytic core domain (CCD) obtained by digestion with papain are monomeric in solution. Secondary structure analysis of ThCBHI by circular dichroism revealed ${\alpha}$- helices and ${\beta}$-strands contents in the 28% and 38% range, respectively. The intrinsic fluorescence emission maximum of 337 nm was accounted for as different degrees of exposure of ThCBHI tryptophan residues to water. Moreover, ThCBHI displayed maximum activity at pH 5.0 and temperature of $50^{\circ}C$ with specific activities against Avicel and p-nitrophenyl-${\beta}$-D-cellobioside of 1.25 U/mg and 1.53 U/mg, respectively.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.124-132
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• 2017

Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an intertidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of $25^{\circ}C$, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implications of the sponge-microbe association in a changing ocean.