• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.201-207
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• 2000

The freshwater green alga Chlorococcum sp. grew on NH_4^{+},{\;}NO_3^{-}$, urea, yeast extract, and peptone as the nitrogen source showing similar pattens of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction fo SC was urea. The dffects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were stydied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20-28 mM urea, 4.8-10 mM phosphate, 1.6 mM sulfate, 70 mM phosphate, 1.6 mM sulfate, 170 mM NACl, and $50{\;}\mu\textrm{M}$ iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improned SC prodution by manipulating the nutrient levels in the modified media were descussed.

• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.118-122
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• 2004

We have investigated that a couple of soft ferrite ceramic powders having a spinal structure have shown the effect on growth and secondary metabolites production of some medicinal plants cultured in vitro. The addition of the ceramic powders as bare state to culture medium has stimulated the growth of Achyranthes japonica callus and plantlet, adventitious root of Hyoscyamus niger and Platycodon grandiflorum hairy root about 65, 75, 150 and 50%, respectively. Whereas Centella asiatica callus and plantlet, Scopolia parviflora hairy root, and Hyoscyamus albus adventitious root were not affected markedly. Moreover, the ceramic powder has enhanced the growth of H. niger adventitious roots even under conditions of irradiating alone without any direct contact between ceramic powder and media. Based on growth stimulation effect, the ceramic powders have enhanced the gross production of tropane alkaloid in H. niger adventitious root, and polyacetylene in P. grandiflorum hairy root about 35 and 30%, respectively.

• Proceedings of the KIEE Conference
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• 2004.05a
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• pp.150-152
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• 2004

The battery charge/discharge process, the final step of the secondary battery production process, requires real-time precision controls for improving both lifetime and performance of the battery cell. In this paper, we present embedded system design for precision control of the secondary battery charge/discharge production process using low power embedded processor based on embedded linux. This system receive charge/discharge command from the main server through ethernet. Compared to existing charge/discharge control system, our design makes low cost and precision control system possible.

• Language and Information
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• v.6 no.1
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• pp.105-121
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• 2002

This paper investigates the effect of semantic argument structure on the comprehension and production of sentences by observing the prosodic realizations of English secondary predications. Specifically, the goal of this study is to show how the theory of predication, argument structure, and focus semantically interact to account for similarities and differences between English resultative and depictive predications. To address this issue, production and comprehension tests were performed. In the fried focus domain (verb phrase), subjects were asked to utter and to comprehend ambiguous sentences in the context monologues. The experimental results were generally consistent with general linguistic analyses: In the resultative constructions, secondary subject NPs tend to be accented, as in other argument-head constructions, while in the depictive constructions, secondary predicates tend to have accents, as in other adjunct-head constructions.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.9
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• pp.1073-1084
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• 1999

The effects of secondary flow on the structure of a turbulent wake generated by a flat plate was investigated experimentally. The secondary flow was induced In a $90^{\circ}$ curved duct in which the flat plate wake generator was installed. The wake generator was installed in such a way that the wake velocity gradient exists in the span wise direction of the curved duct. Measurements were made in the plane containing the mean radius of curvature where pressure gradient and curvature effects were small compared with the secondary flow effect. All six components of the Reynolds stresses were measured in the curved duct. Turbulence intensities in the curved wake are higher than those in the straight wake due to an increase of the turbulent kinetic energy production by the secondary flow. In the inner wake region, shear stress and strain in the plane containing the velocity gradient of the wake show opposite signs with respect to each other, so that eddy viscosity Is negative in this region. This indicates that gradient-diffusion type turbulence models are not appropriate to simulate this type of flow.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.4
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• pp.417-427
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• 1998

The effect of secondary flow on the heat transfer of a turbulent wake generated by a flat plate was experimentally investigated. The secondary flow was induced in a curved duct in which the flat plate wake generator was installed. All three components of turbulent heat flux were measured in the plane containing the mean radius of curvature of the curved duct. The results showed that mean temperature profiles deviate from the similarity of the straight wake because of the cold fluid transported from the free-stream. The half-width of the mean temperature profile increased rapidly by upwash motion of the secondary flow. The changes to turbulence structure caused by the secondary flow show more pronounced effect on heat transport than on momentum transport. This is because the response to the variation of flow conditions is delayed in temperature field. Negative production of the turbulent heat flux is observed in the inner wake region. From the conditional averaging, it has been found that the negative production of the turbulent heat flux is generated due to a mixing process between the hot and low momentum eddies occupied in the inner wake region and the cold and high momentum eddies in the potential region.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.453-458
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• 2000

The paper describes a study of the variation of the NO production emitted from turbulent propane flames established on a practical combustor where a primary and a secondary fuel lines are installed. The flowrate of the secondary fuel is pulsated or added in addition to that of the primary fuel which constrantly flows to the nozzle of the burner. Two modes depending on the positions of supplying the secondary fuel are performed; one is for its position to be placed at the center of the primary fuel tube and the other around the stabilization baffle. The mean concentrations of gas species, $O_2,\;Co\;CO_2,\;NO$, and HC(unburnt hydrocarbones) have been measured at the exit of the combustor. As equivalence ration $({\Phi})$ is increased the profile of the NO concentration on the latter mode rises slowly less than that on the former one. In the range of ${\Phi}=0.5$ to 0.54 the NO production is reduced by about 35% more on the latter mode than on the former one. The influence of pulsating the secondary fuel on the variation of the NO concentration doesn't appear at both modes.

• Korean Journal of Medicinal Crop Science
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• v.20 no.6
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• pp.479-486
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• 2012

The production of adventitious roots derived from root explant of Echinacea angustifolia and its secondary metabolite content were assessed in different types and levels of auxin. The induction of adventitious roots from root explant cultured in Murashige and Skoog solid medium supplemented with 1.0 mg/L indole -3-butyric acid (IBA) attained highest as 20.87 mg fresh weight and 3.07 mg dry weight per culture but root suspension culture at the same concentration of IBA enhanced biomass production as 3.07 g fresh weight and 0.38 g per culture after 4 weeks in culture. 3.0 mg/L ${\alpha}$-naphthalene acetic acid (NAA) treatment had similar effect on root biomass production as 3.07 g fresh weight and 0.38 g per culture with liquid suspension culture, whereas adventitious roots exposed to over 3.0-5.0 mg/L IBA or 5.0 mg/L NAA were less responsive by reducing the number of adventitious roots and/or changing root morphology such as short and thick. The content of secondary metabolites such as phenolic, flavonoids and total caffeic acid in adventitious roots cultured on MS medium supplemented with 1.0 mg/L IBA were attained highest as 27.20, 9.60. 10.67 mg/g dry weight, respectively. Overall, the best production of root biomass and secondary metabolites were given by 1.0 mg/L IBA.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.528-534
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• 2005

Hairy root cultures have demonstrated great promise in terms of their biosynthetic capability toward the production of secondary metabolites, but continue to constitute a major challenge with regard to large-scale cultures. In order to assess the possibility of conducting mass production of biomass, and the extraction of useful metabolites from Panax ginseng. P. ginseng hairy roots, transformed by Rhizobium rhizogenes KCTC 2744, were used in bioreactors of different types and sizes. The most effective mass production of hairy roots was achieved in several differently Sized air bubble bioreactors compared to all other bioreactor types. Hairy root growth was enhanced by aeration, and the production increased with increasing aeration rate in a 1 L bioreactor culture. It was determined that the hairy root growth rate could be substantially enhanced by increases in the aeration rate upto 0.5vvm, but at aeration rates above 0.5vvm, only slight promotions in growth rates were observed. In 20 L air bubble bioreactors, with a variety of inoculum sizes, the hairy roots exhibited the most robust growth rates with an inoculum size of 0.1% (w/v), within the range 0.1 to 0.7% (w/v). The specific growth rates of the hairy root decreased with increases in the inoculum size.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.959-965
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• 2013

Monascus species have been used to produce fermented rice called Monascus-fermented rice (MFR). To improve a Monascus strain via activation of secondary metabolite (SM) gene clusters for use in the production of MFR, we overexpressed an ortholog of the laeA gene, which encodes a global positive regulator of secondary metabolism under the control of the strong heterologous Aspergillus nidulans alcA promoter in Monascus pilosus. The OE::laeA transformant produced more SMs, including those not detected under uninduced conditions. MFR produced using the M. pilosus OE::laeA strain contained 4 times more monacolin K, a cholesterol-lowering agent, than MFR produced using the wild-type strain. In addition, pigment production was remarkably increased, and the antioxidant activity was increased as well. The results from this study suggest that Monascus species, which are important industrial fermentative fungi in Asia, can be improved for the production of functional foods by overexpressing the laeA gene.