• BMB Reports
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• 제56권12호
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• pp.645-650
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• 2023

Numerous studies have investigated the cellular prion protein (PrP^C) since its discovery. These investigations have explained that its structure is predominantly composed of alpha helices and short beta sheet segments, and when its abnormal scrapie isoform (PrPSc) is infected, PrPSc transforms the PrP^C, leading to prion diseases, including Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy in cattle. Given its ubiquitous distribution across a variety of cellular types, the PrP^C manifests a diverse range of biological functions, including cell-cell adhesion, neuroprotection, signalings, and oxidative stress response. PrP^C is also expressed in immune tissues, and its functions in these tissues include the activation of immune cells and the formation of secondary lymphoid tissues, such as the spleen and lymph nodes. Moreover, high expression of PrP^C in immune cells plays a crucial role in the pathogenesis of prion diseases. In addition, it affects inflammation and the development and progression of cancer via various mechanisms. In this review, we discuss the studies on the role of PrP^C from various immunological perspectives.

• The Plant Pathology Journal
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• 제40권2호
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• pp.225-232
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• 2024

The microbiomes of two important rice cultivars in Vietnam which differ by their susceptibility to the bacterial leaf blight (BLB) disease were analyzed through 16S rRNA amplicon technology. A higher number of operational taxonomic units and alpha-diversity indices were shown in the BLB-resistant LA cultivar than in the BLB-susceptible TB cultivar. The BLB pathogen Xanthomonas was scantly found (0.003%) in the LA cultivar, whereas was in a significantly higher ratio in the TB cultivar (1.82%), reflecting the susceptibility to BLB of these cultivars. Of special interest was the genus Acholeplasma presented in the BLB-resistant LA cultivar at a high relative abundance (22.32%), however, was minor in the BLB-sensitive TB cultivar (0.09%), raising a question about its roles in controlling the Xanthomonas low in the LA cultivar. It is proposed that Acholeplasma once entered the host plant would hamper other phytopathogens, i.e. Xanthomonas, by yet unknown mechanisms, of which the triggering of the host plants to produce secondary metabolites against pathogens could be a testable hypothesis.

• 대한영상의학회지
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• 제84권3호
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• pp.565-585
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• 2023

항문주위 샛길은 일반 인구에서 매우 흔하고 항문관 주변 부위에 영향을 미치는 염증성 질환이다. 대부분 양성이지만 심각한 이환율을 유발하고 재발 위험이 높아 외과적 치료가 필요하다. 자기공명(MR) 영상은 항문관의 해부학적 구조, 항문조임근 복합체와의 관계, 2차 경로 또는 농양의 정확한 식별에 대한 정확한 정보를 제공하고 관련 합병증을 보고하는 항문주위 샛길 평가를 위한 최적 표준 기술로 간주된다. MR 영상은 또한 치료 효과를 모니터링하고 치료 방법을 결정하기 위한 정확한 정보를 제공할 수 있다. 크론병 관련 샛길은 종종 질병을 완화하기 위해 외과적 치료보다는 내과적 치료가 필요하다. 영상의학과 의사는 샛길의 해부학적 구조와 MR 영상 소견을 알아야 하고 임상의사에게 정확한 진단을 제시할 수 있어야 한다.

• 생명과학회지
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• 제27권1호
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• pp.67-71
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• 2017

부저병이란 Paenibacillus larvae감염에 의하여 꿀벌 유충의 괴사를 유도하는 질병이다. 우리나라에서는 2008년 봄, 국내에 처음으로 대량 발병 사례가 보고되었으며, 지속적인 2차 피해로 큰 후유증을 앓고 있다. 본 연구에서는 부저병을 효율적으로 관리할수 있는 진단방법을 조사하고자 하였다. 따라서 부저병의 원인균인 P. larvae에서 특이적으로 발현되고 있는 유전자들을 동정하고자 하였으며, 이 유전자들은 주로 부저병균의 독성을 유발하는 것으로 알려진 Toxin1, Toxin2A & 2B, SplA, CBP49, SevA&SevB 들이다. 이들은 1차 PCR 에서는 검출하기 어려웠지만, 2차 nested PCR방법을 이용하여 검출이 용이함을 알 수 있었다. 한편 여러가지 식물 추출물을 혼합한 배지에서 부저병균을 배양하였을 때, 부저병균의 성장저해와 일치하게 우리가 검증한 유전자들의 발현이 감소하는 것을 알 수 있었다. 이러한 결과들은 부저병 원인균의 특이 유전자들은 향후 PCR진단마커로서 활용 가능성이 있을 것으로 사료된다.

• 대한한의학원전학회지
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• 제31권4호
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• pp.79-90
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• 2018

Objectives : In present, various definitions of Zhongshu are being used interchangeably in Korea. The paper attempted to discover the disease of Zhongshu as studied by Lidongyuan, who was ahead of his time. Methods : A database of medical books has been studied to find Lidongyuan's writings on the disease of Zhongshu, and mentions about Li's works in past medical books. The paper contemplated the subject by defining the disease of Zhongshu and reviewing the disputes related to the subject. Results & Conclusions : Lidongyuan gave a detailed explanation on the fact that Zhangjiegu distinguished between Zhongshu and Zhongre, and this was influenced by Shangshu as mentioned in Taipinghuiminhejijufang. Therefore, it can be deduced that he was aware of the fact that summerheat-heat as latent summer heat syndrome is lurking inside the body, not being able to be released. According to the disease of Zhongshu by Lidongyuan, yin cold was receieved secondary after first receiving summerheat-heat. It is either summerheat damaged defensive qi, failure in storing the essence made defensive qi weak, or seasonal reasons have caused the defensive qi to be drained and leave the body exposed to damage by summerheat. This is because the fundamental main cause is the hitting of summerheat, since yin cold was received after the presence of summerheat-heat is made first. Many doctors in the following generation criticized that Lidongyuan's disease of Zhongshu cannot be named as a Zhongshu due to its similarity with Shanghan. However, they cannot be viewed as similar since it is a phenomenon where yin cold becomes congested while the body is weak and heat is generated in the body due to summerheat-heat. The doctors who said they were similar only focused on the external cause that was only the superficial issue. According to Lidongyuan's method, the right way to treat a Zhongshu disease is to use the method of tonifying the qi and eliminating the heat in conjunction with eliminating the internal dampness or treat the external syndrome, or to use a formula to tonify the qi and eliminate the fire heat before eliminating the internal dampness or treating the external syndrome.

• 동국한의학연구소논문집
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• 제8권2호
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• pp.47-67
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• 2000

근골격계 질환은 임상에서 가장 흔하게 접할 수 있는 대상의 하나로 그 진단과 평가가 매우 중요하며 여러 가지 진단기기와 평가방법이 이용되고 있다. Thermography는 기존의 진단기기와 달리 질병의 기능적인 측면을 평가할 수 있다는 점과 통증과 같은 주관적인 표현을 어느정도 객관화할 수 있다는 점에서 임상적 가치가 고려되고 있다. 본 연구는 최근 국내외의 임상 논문을 대상으로 근골격계 영역에서 Thermography의 활용 동향을 고찰하여 진단적 가치를 확인하고자 하였다. Thermography는 척추신경병증, 근막동통증후군, 말초신경병증, 반사성 교감신경 이영양증, 레이노 증후군, 악관절 장애 및 일부 기타 질환에서 적용되었으며, 대부분의 질환에서 보조적인 진단기기로 활용가치가 있었다. 특히, 질병의 경과 관찰과 약물 및 기타 처치에 대한 효과 판정에 유용하였으며, 레이노 증후군 및 반사성 교감신경 이영양증과 같은 질병에서는 일차적인 진단기기로도 활용이 가능하였다. 향후 근골격계 영역에서 Thermography의 보다 폭넓은 적용을 위해서는 질환에 따른 적절한 촬영방법의 개발 및 결과 판독에 대한 객관성 확보가 있어야 할 것이다.

• Molecules and Cells
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• 제26권2호
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• pp.206-211
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• 2008

HI0381 of Haemophilus influenzae was investigated by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. HI0381 is a 153-residue peptidoglycan-associated outer membrane lipoprotein, and a part of the larger Tol/Pal network. Here, we report its backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignments, and secondary structure predictions. About 97% of all of the $^1HN$, $^{15}N$, $^{13}CO$, $^{13}C{\alpha}$, and $^{13}C{\beta}$ resonances covering 131 non-proline residues of the 134 residue, mature protein, were clarified by sequential and specific assignments. CSI and TALOS analyses revealed that HI0381 contains five ${\alpha}$-helices and five ${\beta}$-strands. To characterize the structure of HI0381, the effects of pH and salt concentration were investigated by CD. In addition, the structural changes occurring when HI0381 was in a membranous environment were investigated by comparing its HSQC spectra and CD data in buffer and in DPC micelles; the results showed that helix ${\alpha}4$ and strand ${\beta}4$ became aligned with the membrane. We conclude that the conformation of HI0381 is affected by the membrane environment, implying that its folded state is directly related to its function.

• 간호행정학회지
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• 제6권2호
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• pp.195-209
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• 2000

The purpose of this study is to estimate the population requiring nursing home services among elderly people in Korea. This study identifies the need of nursing home services determined by health care professionals and estimates the proportion of elderly people requiring nursing home service according to the admission criteria. Surveys were conducted on health care professionals including medical doctors, home care nurses, and nurse practitioners. They were asked to assess nursing home need based on four content areas: Physical function (Activities of Daily Living), chronic disease, Physical symptoms (incontinence), mobility, eating, and sensory function. Based on the professionally determined need criteria the proportion of elderly people requiring nursing home services was estimated using secondary data from the 1994 Survey on the Living Status of the Korean Elderly. The number of study subjects to estimate nursing home need who were 60 and older totaled 2,058. The most important factor contributing to the admission eligibility criteria was the elderly living alone. Other factors related were the elderly being unable or having difficulty carrying out activities, and having insufficient help from other our activities, and having insufficient help from other members of the household. Using only physical function, the proportion of elderly people requiring nursing home was $8{\sim}9%$. When only chronic disease was used, proportions varied widely; for the doctor's group, the proportion was over 30%. Using all areas, the proportions of elderly people requiring nursing home were between 13% and 38%. The estimate using chronic disease and physical function was similar to the on using all areas.

• 한국동물위생학회지
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• 제38권2호
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• pp.89-94
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• 2015

Respiratory disease in pigs is common in modern pork production worldwide and is often referred to as porcine respiratory disease complex (PRDC). PRDC is polymicrobial in nature, and results from infection with various combinations of primary and secondary respiratory pathogens. The control of swine respiratory disease requires an understanding of the interactions between the organisms that can cause this illness, the pig and management of the environment. This study was carried out to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. A total of 900 lung samples, 45 farms were collected randomly from slaughtered pigs in Jeonbuk province from April to December in 2014. Gross lesions such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis, liver white spots were examined for the pigs. Overall prevalence of SEP was 70.8%. According to season, the incidence occurred higher in summer than winter, fall and spring. The mean SEP score was 1.4, the highest incidence occurred in fall. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 26.1%, 71.4%, 2.8%, 21.6%, respectively. In the detection of pathogens, PRRS was not detected, PCV2 was positive in 87.6%.

• 대한한방내과학회지
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• 제26권4호
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• pp.926-934
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• 2005

It is well known that NERD(Non-erosive gastroesophageal reflux disease) is a well known esophageal disease. Symptoms of NERD may be divided into two classes. Important symptoms are acid regurgitation and heartburn. Secondary symptoms are noncardiac chest pain, dysphagia, chronic coughing, hoarseness, sore throat, globus, halitosis, epigastric pain or discomfort and abdominal pain or discomfort related to bowel movement. The relationship between NERD and Tan-San(呑酸) is complex. But the most common symptoms of NERD are acid regurgitation and heartburn. These symptoms arc similar to Tan-San and can be treated with oriental medical herb and acupuncture. Ljintang-Gamibang(二陳湯加味方) mentioned in the Donguibogam(東醫寶鑑) is one of these oriental medical herbs. Therefore, this comparative study between first medical examination and end of medical treatment with Ljintang-Gamibang and acupuncture was made. The clinical progress of NERD was investigated. After treatment, all seven patients showed improvement in all symptoms associated with NERD, including general condition. The results of this study suggest that Ljintang-Gamibang and acupuncture are an effective treatment for NERD and progressive research to develop a practical treatment of NERD are needed.