• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.1
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• pp.30-34
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• 2012

The filamentous bacterium Leucothrix mucor, an epiphyte of seaweed, showed antifouling activity against Ulva pertusa spore settlement and germling development. The chemical constituents representing the antifouling activity were identified as giffinisterone B and oleamide based on nuclear magnetic resonance (NMR) spectroscopy and mass spectroscopy (MS). Approximately 3.6 mg of giffinisterone B and 2.8 mg of oleamide were isolated from 1.6 g of Leucothrix mucor crude extract. Giffinisterone B fully inhibited spore settlement and germling development at $100{\mu}g/mL$. Oleamide inhibited spore settlement at $10{\mu}g/mL$ and germling development at $100{\mu}g/mL$.

• Journal of Practical Agriculture & Fisheries Research
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• v.17 no.1
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• pp.45-56
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• 2015

The distribution of seaweed was examined in Jeonnam archipelago marine ranching area, the coastal of geumo-do, Yosu. Abundance and distribution of seaweed in dropping site of artificial reef were sampled at 6 station at October 2007. Seaweed community were investigated with quadrat method at line-transect by scientific SCUBA divers. as a result, 30 species in total, 4 green, 9 brown and 15 red seaweed were identified. Range of seaweed biomass in all sampling stations were about 1,600~4,000 g/m². At intertidal zone, appearance of individuals and dominance rate were showed higher than below the subtidal line and at depth from 4m to 6m, individuals, dominance rate and biomass were represented low level. characteristic of water in marine ranching area is turbidity and also compensation depth is low. For that reason, individuals of seaweed community were smaller from depth of 4 meters. Therefore, when equip the artificial reef for preparation of seaweed beds need consider that equipment periods(early spring; before releasing marine seaweed spore) and suitable water depth(3~6m).

• Journal of Life Science
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• v.26 no.4
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• pp.484-489
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• 2016

Living organisms can maintain or extend their territories by producing allelochemicals that influence the growth, survival, and reproduction of other organisms. To identify natural biostimulants of positive allelochemicals, we screened 18 common seaweed extracts for enhancement of rhizoid and blade production in a convenient Porphyra suborbiculata monospore assay. By addition of methanolic extract from the most potent green seaweed, Codium fragile, 100% and 50% enhancement doses reflecting the amount of C. fragile extract required to enhance rhizoid formation (in terms of number of spores with rhizoids per total spores tested) were approximately 100 and 50 μg/ml, respectively, in the P. suborbiculata monospore culture. The C. fragile extract quickly enhanced rhizoid formation, rhizoid numbers per rhizoid-holding spore, rhizoid length, blade formation (in number of spores with blade per total spores tested), and blade length from most monospores at early culture days. The extract enhanced rhizoid formation after 2 days of culture significantly, rhizoid numbers per rhizoid-holding spore after 3 days, rhizoid length after 3 days, blade formation after 2 days, and blade length after 1 day, respectively, from most monospores. The allelochemicals that enhanced favorite seaweed species may be efficacious for new seaweed management technologies, including the development of biostimulant agents based on natural products.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.151-152
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• 2000

During in door or outdoor mass culture, Porphyra have been easily contaminated with bacteria, protozoa and microalgal species. Several axenic treatments for Porphyra thalli have been published (Polne-Fuller and Gibo 1984; Chen and McCracken 1993), but axenic techniques for neutral spores and conchocelis we not developed. In this work we describe the procedure for axenic isolation of neutral spores and conchocelis of Porphyra yezoensis (omitted)

• Korean Journal of Fisheries and Aquatic Sciences
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• v.2 no.1
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• pp.83-86
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• 1969

The present investigations were made with the purpose of elucidating the seasonal variation in total nitrogen content of Gelidium amansii. Monthly samples were collected from the sea near pusan, from August 1957 to June 1959. The results obtained have been summarized as follows: The maximum total nitrogen content of Gelidium amansii was observed during the months of January and February, and the minimum content was observed in June and July Thus the total nitrogen content of Gelidium amansii exhibited a considerable periodic change throughout the year. The recommended harvest time of Gelidium amansii with regard to total nitrogen content, is from May to October, but on the other hand the proper harvest time is from May to June, with respect to the season of spore-formation and its propagation.

• Journal of Ocean Engineering and Technology
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• v.22 no.3
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• pp.103-108
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• 2008

Topics discussed in this paper are engineering design of artificial reefs, design considerations of compressive strength and pH, materials of silica fume concrete and volcanic stones for reef construction, and field monitoring of artificial reefs. Six artificial reefs were deployed offshore at Biyangdo in July 2006, and succession patterns on the reefs were investigated bimonthly from July 2006 to October 2007. The spore-bag method, which has been widely used in Korea for the attachment of seaweed on artificial reefs, was applied to the developed reefs using the kelp Ecklonia cava, which is the dominant species in Jeju, and recruitment and growth patterns were observed.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.406-416
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• 2019

Strawberry, an important fruit crop, is susceptible to a large number of pathogens that reduce fruit quality and productivity. In this study, the effect of a biostimulant prepared from Ascophyllum nodosum extract (ANE) (0.1%, 0.2%, and 0.3%) was evaluated on powdery mildew progression under greenhouse and field conditions. In the greenhouse, application of 0.2% ANE showed maximum reduction in powdery mildew progression as compared to the control. Forty-eight hour post-inoculation, foliar spray of 0.2% ANE reduced spore germination by 75%. Strawberry leaves sprayed with ANE showed higher total phenolic and flavonoid content in response to powdery mildew infection. Furthermore, application of ANE elicited defense response in strawberry plants by induction of defense-related enzymes, such as phenylalanine ammonia lyase, polyphenol oxidase, and peroxidase activity. In field conditions, foliar spray of 0.2% ANE showed a reduction of 37.2% of natural incidence of powdery mildew infection as compared to the control. ANE sprayed plant also reduces the severity of powdery mildew infection under natural conditions. These results indicate that application of ANE induces the strawberry plant's active defense against powdery mildew infection by induction of secondary metabolism and regulating the activities of defense-related enzymes.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.567-582
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• 2015

Botrytis cinerea infects stems, leaves and fruits of greenhouse tomato and can cause serious economic losses. This study was conducted to develop organic farming control method against tomato gray mold. Twenty two organic farming materials including mineral and plant extracts were screened for the suppressive activity against Botrytis cinerea, in vitro and in vivo. Among the organic farming materials, sulfur, copper, Chinese twinleaf extract and rhubarb extract decreased by 51.7-90% of the spore germination of Botrytis cinerea. Also, gray mold incidence was reduced more than 90% on tomato stems by treating sulfur, seaweed extracts, rhubarb root extracts and Chinese twinleaf extract. After the selected four organic farming materials were applied on tomato cultivated in greenhouse, their control effects against the tomato gray mold were tested. When the water soluble sulfur was foliar-sprayed on the tomato leaves infected by artificial inoculation with spore suspension of Botrytis cinerea, it showed 87.9% of control value. Also, control activity of the water soluble sulfur was paralleled with chemical fungicide, diethofencarb+carbendazim. The above mentioned results indicate the sulfur formulation can be used as chemical fungicide alternatives for controlling tomato gray mold in the greenhouse.

• Ocean and Polar Research
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• v.42 no.2
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• pp.133-139
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• 2020

The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35℃) and three light intensity levels (20, 60, and 100 µmol photons m^-2 s^-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08-28.38% day^-1 for tetrasporophytes and 11.58-23.67% day^-1 for gametophytes. At 35℃, holdfasts of tetrasporophytes survived with RGRs of 7.08-23.28% day^-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30℃ and 100 µmol photons m^-2 s^-1, which were different from gametophytes (25℃ and 100 µmol photons m^-2 s^-1). RGRs of tetrasporophytic sporelings were 2.93-11.11% day-1 and were between 0.78-10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25℃ and 60 µmol photons m^-2 s^-1 for tetrasporophytes, and at 20℃ and 100 µmol photons m^-2 s^-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.1
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• pp.93-102
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• 2020

This study investigated the dif usion characteristics of Ecklonia cava spores around marine forest reefs. For this purpose, a numerical analysis was conducted using field observations in the target area and an EFDC model. Based on the results of field observations and the EFDC model, Flow-3D was performed on three types of marine forest reefs, mamely triangular pyramid reef, double-dome reef, and ribbed reef, to monitor the movement direction and maximum movement distance for E. cava spores. As a result, the equilaterally triangular pyramid reef and double-domed reef were found to have a maximum settlement of spores of 10 m in the northwestern direction and 6 m in the western direction. The ribbed reef had a maximum settlement of spores at 4m. These results show that consideration of the diffusion characteristics of E. cava spores when the seaweeds are installed can increase the effectiveness of the algae as substrate of adhesion.