• 한국수산과학회지
• /
• 제44권1호
• /
• pp.1-7
• /
• 2011

This study examined Tetraselmis sp. JK-46 isolated from seawater from the East Sea. Deep seawater (DSW) had a greater effect on the growth of Tetraselmis sp. JK-46 than surface seawater (SSW). The crude protein, lipid, carbohydrate and ash contents of Tetraselmis sp. JK-46 cultured with DSW were 27.2, 37.1, 13.2 and 26.3 %, respectively, and these values were similar to the results for samples cultured with SSW. The contents of Mg, Ca, Fe and K in the DSW cultured samples were 7080.3, 1009.6, 251.2, and 2749.7 mg/100 g, respectively. The fatty acid compositions of Tetraselmis sp. JK-46 cultured with DSW and SSW were 53.7 and 49.0 % polyunsaturated fatty acids (PUFA) and 25.7 and 30.7 % saturated fatty acids (SFA), respectively. The total amino acid contents of the samples cultured with DSW and SSW were 7392.6 and 6376.0 mg/100 g respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of Tetraselmis sp. JK-46 extracts increased with the concentration of the chloroform and ethyl acetate fractions. The half maximal inhibitiory concentrations ($IC_{50}$) of the chloroform and ethyl acetate fractions of DSW and SSW cultured samples were 1.2 and 2.6 mg/mL, and 3.1 and 3.3 mg/mL, respectively. The ethyl acetate fractions of DSW and SSW cultured samples has anticoagulant activity and the activated partial thromboplastin times (APTT) were 93.4 and 89.3 sec., respectively. The chloroform and ethyl acetate fractions showed antimicrobial activity against Bacillus subtilis, Escherichia coli and Candida albicans.

• ALGAE
• /
• 제17권2호
• /
• pp.127-133
• /
• 2002

The red seaweed Eucheuma serra is a high yielding source of lectins. The plants were collected from a depth of 5-6 meters and cultured in the laboratory, field and deep seawater. A Daily Growht Rate (DGR) of 3.5% was observed at 18℃ with a low light of 30μmol photon $ m^{-2} · s^{_1}$ in the laboratory. When the plants were cultured in the field at different depths during winter onths of December and January, best growth was observed at 1 m depth and a DGR of 2.14±0.04% was recorded. The plants grown in the tank with a continuous supply of deep seawater showed a DGR of 8.2% The results indicate that E. serra can be cultivated in large scale both in deep seawater in the tank and in the field for the extraction of lectins at a commercial scale.

• 한국수산과학회지
• /
• 제23권4호
• /
• pp.334-338
• /
• 1990

틸라피아를 담수와 해수에서 각각 6개월간 사육하여, 이들의 일반성분과 정미성분을 분석 $\cdot$비교하였다. 일반성분에서는 해수사육한 것이 담수사육한 것에 비해 수분함량과 단백질 양에는 거의 차이가 없었으며, 지질함량과 회분함량은 약간 많았다. 또 한 정미성분중 유리아미노산인 taurine과 glycine, 핵산관련물질인 IMP, total creatinine 등의 함량이 많았으며, 이들은 함량면에서 보아 틸라피아의 맛에 크게 기여할 것으로 생각된다. 그러나 함질소화합물의 함량이나 조성에서는 해수사육과 담수사육간에 별다른 차이를 확인할 수 없었다.

• Journal of Microbiology and Biotechnology
• /
• 제19권10호
• /
• pp.1206-1212
• /
• 2009

Growth rates, photosystem II photosynthesis, and the levels of chlorophyll $\alpha$ and secondary metabolites of Chlorella ovalis were estimated to determine if they were enhanced by the addition of swine urine (BM) or cow compost water (EP) that had been fermented by soil bacteria to deep seawater (DSW) in an attempt to develop media that enabled batch mass culture at lower costs. Growth of C. ovalis in f/2, f/2-EDTA+BM60%, DSW+BM30%, and DSW+EP60% was enhanced and maintained in the log phase of growth for 16 days. The cell densities of C. ovalis in DSW+EP60% ($4.1{\times}10^6$ Cells/ml) were higher than those of f/2 ($2.9{\times}10^6$ Cells/ml), f/2-E+BM60% ($3.7{\times}10^6$ Cells/ml), and DSW+BM30% ($2.7{\times}10^6$ Cells/ml). The growth rate was also more favorable for C. ovalis cultured in DSW+EP60% ($0.15\;day^{-1}$) than that of C. ovalis cultured in the control medium (f/2) ($0.12\;day^{-1}$). Furthermore, the chlorophyll a concentration of C. ovalis cultured in DSW+EP60% (4.56 mg/l) was more than 2-fold greater than that of C. ovalis cultured in f/2 (2.35 mg/l). Moreover, the maximal quantum yields of photo system II at 470 nm (Fv/Fm) were significantly higher in organisms cultured at f/2-E+BM60% (0.53) and DSW+EP60% (0.52) than in the other treatment groups. Finally, Fourier transformation infrared (FT-IR) spectroscopy revealed that C. ovalis grown in DSW+EP60% had more typical peaks and various biochemical pool shifts than those grown in other types of media. Taken together, the results of this study indicate that the use of DSW+EP60% to culture C. ovalis can reduce maintenance expenses and promote higher yields.

• Fisheries and Aquatic Sciences
• /
• 제3권2호
• /
• pp.118-125
• /
• 2000

Lipid classes and fatty acid compositions of eight species of wild and cultured seawater fish in Korea were investigated. Total lipid (TL) contents of wild and cultured fish were $2.64\pm1.88\%$ and $5.42\pm1.76\%$, respectively, except for rockfish and striped beakperch. Non-polar lipids (NL) in all fish samples comprised approximately $84\%$ of the TL content. The proportion equation of NL content to TL content was y=0.9296x-0.4468 $(R^2=0.98l2, p<0.001)$. The most abundant NL class was triglyceride. The prominent fatty acids in all fish samples were 16: 0, 18: 1(n-9), 22 : 6(n-3) (docosahexaenoic acid, DHA), 16: 1(n-7), 20 : 5 (n-3) (eicosapentaenoic acid, EPA), 18 : 0 and 18 : 1(n-7). The polyunsaturated fatty acid (PUFA) group was the richest among the total fatty acids (TFA). DHA and EPA were comprised of approximately $85\%$ of n-3 PUFA. There was a positive correlation between TFA content and n-3 PUFA content; y=0.292x-0.0055 $(R^2=0.9349, p<0.001)$. The n-3 PUFA content of the cultured fish was approximately twice as much as that of the wild fish. Therefore, cultured fish were proven to provide better sources of n-3 PUFA if compared to wild fish.

• 한국수산과학회지
• /
• 제51권3호
• /
• pp.254-261
• /
• 2018

From 2014 to 2017, mortalities of seawater-cultured rainbow trout Oncorhynchus mykiss, were observed in the Goheung and Jeju areas of Korea, with Vibrio anguillarum (seven strains: RT1, 2, 3, 4, 5, 6, and 7) identified as the etiological agent. The phenotypic (based on API 20NE, API ZYM, and E-test kits), serotypic (slide agglutination tests with O1, O2, O3, O4, and O7 antisera), and genotypic (16S rRNA and ompU sequencing) characteristics of the seven RT strains were analyzed and compared to those of seven additional V. anguillarum stains (SF, isolated from sweet fish; FM, isolated from flathead mullet; ATCC43305; ATCC43311; ATCC43307; ATCC43308; and KCTC2711). The phenotypes of the RT strains showed variance, while the slide agglutination tests of the RT1-7, SF, and FM strains all showed positive reactions with serotype O1 antiserum. The 16S rRNA and ompU sequences of the RT1-7, SF, and FM strains were affiliated with V. anguillarum ATCC43305 (Serotype O1), but the ompU sequence of the SF strain differed from those of the RT1-7, FM, and ATCC43311 strains, including one amino acid substitution. We thus confirmed that serotype O1 V. anguillarum, with multiple phenotypes, continues to infect seawater-cultured rainbow trout in Korea.

• ALGAE
• /
• 제18권2호
• /
• pp.129-134
• /
• 2003

In order to examine the effects of deep seawater (mesopelagic water in the broad sense) on the growth of macroalgae, the growth and nutrient uptake (nitrate and phosphate) of Ulva sp. (Ulvophyceae, Chlorophyta) were investigated by cultivation in deep seawater (taken from 687 m depth at Yaizu, central Japan, in August 2001), surface seawater (taken from 24 m depth), and a combination of the two. Culture experiments were carried out in a continuous water supply system and an intermittent water supply system, in which aerated 500-mL flasks with 4 discs of Ulva sp. (cut sections of ca. 2 $cm_2$) were cultured at 20$^{\circ}C$ water temperature, 100 $\mu$mol photons $m^{-2}{\cdot}s^{-1}$ light intensity, and a 14:10 light:dark cycle. Nutrient uptake by Ulva sp. was high in all seawater media in both culture systems. The frond area, dry weight, chlorophyll a content, dry weight per unit area, and chlorophyll a content per unit area of Ulva sp. at the end of the experimental period were the highest in deep seawater and the lowest in surface seawater in both culture systems. These values, except for dry weight per unit area and chlorophyll a content per unit area, for each seawater media in the intermittent water supply system were higher than those in the continuous water supply system. We conclude that not only deep seawater as the culture medium but also the seawater supply system is important for effective cultivation of macroalgae.

• 한국발생생물학회지:발생과생식
• /
• 제13권1호
• /
• pp.35-41
• /
• 2009

겨울철 사육조건이 양식산 뱀장어 Anguilla japonica의 인위적인 성성숙 유도 및 번식에 미치는 영향을 조사하였다. 봄철 salmon pituitary extracts(SPE)을 이용하여 성성숙 유도 예정인 양식산 암컷 뱀장어 친어를 8주의 겨울철 사육기간 동안, 4개의 사육환경(해수저온, $10^{\circ}C$; 해수고온, $20^{\circ}C$; 담수저온, $10^{\circ}C$; 담수고온, $20^{\circ}C$)에 각각 순치하여 사육하였다. 그 후 각각의 실험구의 뱀장어를 해수 $20^{\circ}C$로 이동하여 SPE를 매주 1회 8주 동안 복강 주사하였다. 그 결과, 생식소증량지수(GSI)와 성호르몬(E2)의 농도가 해수 실험구에서 유의적으로 증가하였으며, 특히 해수저온 실험구에서 가장 높은 값을 나타냈었다. 번식률 또한 해수저온 실험구에서 가장 높게 나타났다. 이러한 결과는 겨울철 사육환경이 봄철의 성성숙 유도에 효과적인 것으로 추측되며, 양식산 뱀장어의 성성숙 유도와 부화율 향상을 위한 기초자료로 유용할 것으로 사료된다.

• 한국양식학회지
• /
• 제13권1호
• /
• pp.39-46
• /
• 2000

우리나라의 대하양식은 먹이생물이 대체로 풍부한 연안 및 만을 중심으로 제방을 쌓거나 폐쇄된 염전 등을 이용하여 극히 한정된 장송에서 실시되고 있다. 본 조사 대하 양식장인 남해의 갈화 양식장은 조석차이를 이용한 해수교환이 주기적으로 실시되고 있으나, 서해의 운호 양식장은 단지 양수에 의한 해수보충이 가끔 실시되고 있을 뿐이다. 따라서, 본 연구는 갈화와 운호양식장의 환경특성을 파악하였고, 이에 따른 성장을 비교.검토하다. 갈화양식자의 저질성상은 사질이 약 70%를 차지한데 비해 운호 양식장은 대부분 펄이 차지하고 있었다. 저질중의 황화물은 갈화 양식장에서 1.8mgS/g-dry, 운호 양식장에서 4.7mgS/g-dry가 측정되어 약 3배의 차이를 나타내었다. 해수중의 ammonia-N 및 $H_2S$ 농도는 갈화 양식장에서 각각 0.10mg/${\ell}$ 및 10.8 ${\mu}$g/${\ell}$가 측정된 반면, 운호 양식장에서는 약 2배 이상 높은 0.31mg/l 및 21.2${\mu}$g/${\ell}$이 측정되었다. 또한, DO는 7월부터 8월에 걸쳐 갈화 양식장에서 6.0mg/${\ell}$이하, 운호 양식장에서는 5.0mg/${\ell}$이하가 관찰되었다. 대하의 체장과 체중은 10월 갈화 양식장의 158.2mm 및 28.9g에 비해 운호 양식장에서는 138.3mm 및 22.9g로 유의하게 낮은 성장을 보였다. 또한, 대하의 일일 성장률도 양식기간을 통해 갈화 양식장에 비해 운호 양식장에서 낮게 나타났다/ 따라서, 운호 양식장의 저층수에서 측정된 ammonia-N, $H_2S$ 및 DO는 대하의 성장에 영향을 미쳤다는 것을 의미한다.

• 대한의생명과학회지
• /
• 제22권4호
• /
• pp.150-159
• /
• 2016

Knowledge of the distribution and biodiversity of environmental bacteria and the ecosystem that influences them is crucial for predicting an ecosystem. However, bacterial culture methods can only analyze approximately 0.1% of the existing microorganisms, those that are readily cultured under laboratory conditions. By contrast, next-generation sequencing (NGS) has generally been known to obtain more diverse profiling of bacterial composition. We compared the bacterial communities using both a culture-dependent (MALDI-TOF) and culture-independent (NGS) methods. Environmental specimens were obtained from both freshwater and seawater. Water samples were also analyzed by both pyrosequencing and MiSeq sequencing, in order to select one NGS platform which could analyze comparatively more diverse microbiota. Bacterial distribution analyzed with MALDI-TOF showed no difference between the microbiota of freshwater and seawater, whereas the results analyzed with NGS distinguished between the two. The diversity indexes of MiSeq sequencing were higher than for Pyrosequencing. This indicated that MiSeq sequencing is capable of analyzing a comparatively wider diversity of bacteria. The genus of Flavobacterium and Planktophila were identified as being unique to freshwater, whereas EU801223 and OM43 were found in the seawater. Difference between the bacterial composition of the freshwater and seawater environments was identified by MiSeq sequencing analysis.