• Title/Summary/Keyword: Seawater culture

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Optimal Culture Conditions for Photosynthetic Microalgae Nannochloropsis oculata (광합성 미세조류 Nannochloropsis oculata의 최적배양 조건)

  • Park, Hyun-Jin;Jin, Eun-Jung;Jung, Tae-Man;Joo, Hyun;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.21 no.6
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    • pp.659-663
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    • 2010
  • Microalgae has been seen all over the seawater and several species are used for human food. Specially, Nannochloropsis oculata, a photosynthetic microalgae, has been focused for a vast array of valuable nutritious compounds. In order to find high mass Nannochloropsis oculata culture conditions, some of important growth factors of pH, temperature, culture media, and $CO_2$ effect were tested. The optimal growth condition was found to be as follows : 3% artificial seawater, initial pH 8.5, and temperature $25^{\circ}C$. The alga mass and chlorophyll content were dramatically increased by applying 5% flue $CO_2$ gas (1.50 g/L algae in a continuous $CO_2$ flue; 0.76 g/L alga without $CO_2$). It was shown that the chlorophyll biosynthesis was also closely associated with alga growth.

Performance of a Lab-Scale Closed Seawater Recirculating System for Korean Rockfish Sebastes schlegeli Culture

  • Peng, Lei;Oh, Sung-Yong;Jo, Jae-Yoon
    • Ocean and Polar Research
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    • v.25 no.4
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    • pp.493-501
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    • 2003
  • Performance of a laboratory scale closed seawater recirculating aquaculture system was evaluated. Twenty-kg of korean rockfish (130 fish) with an average body weight of 153.8g was stocked. Over a 107-day culture period, fish reached final density of $51.7kg/m^3$ (initial density, $33.3kg/m^3$) on the basis of the culture tank volume. On a daily basis, added water amounted to 3.4% of the total water volume in the system. Total ammonia nitrogen (TAN) concentrations were below 1mg/l and nitrite nitrogen $(NO_2-N)$ concentrations were within the range of 1-3mg/l on most sampling days. TAN was removed from bead and sand filters and it was removed or produced in the sedimentation basin. Basically, $NO_2-N$ was removed in the bead and sand filters, while it was either removed or produced in the sedimentation basin. Nitrate nitrogen $(NO_3-N)$ was produced in the bead filters and removed from the sand filter and sedimentation basin. The foam fractionator performed well in the recirculating system. The maximal daily removal values for total suspended solids (755) and protein were 10.9g and 1.4g, respectively. Whole water quality parameters were within the levels commonly recommended for fish culture on most of the sampling days. However, further studies are needed to evaluate the commercial feasibility of this system because of the smallscale system used in present experiment. At least, the present study still provides some basic information for further studies of this kind of system.

Acute Toxicity to Peptone Concentrations in the Polychaete Perinereis aibuhitensis under Laboratory Culture

  • Kang, Kyoung-Ho;Zhang, Litao;Ahn, Sam-Young;Kahng, Hyung-Yeel;Zhang, Zhifeng;Sui, Zhenghong
    • Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.205-209
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    • 2011
  • Organic pollution causes eutrophication and dystrophication, which occur when excessive amounts of organic matter enters seawater. Eutrophication can contaminate sediment and harm aquaculture. Polychaeta species have been shown to restore eutrophic sediment. In this study, we used peptone to simulate a eutrophic environment and detect the levels at which eutrophication became toxic to the polychaete Perinereis aibuhitensis. Peptone concentrations were 0, 100, 200, and 500 mg/L. The median lethal concentrations were 950.35 mg/L at 48 h, 340.34 mg/L at 72 h, and 120.22 mg/L at 96 h, which are much higher than those of other aquatic species. Polychaeta species are highly tolerant of eutrophication. During the 15-day long-term experiment, sediment loss on ignition, as well as seawater total organic carbon and total nitrogen all decreased significantly (P<0.05). However, $NH_4^+$ concentration increased with time. Perinereis aibuhitensis slowed the increment of $NH_4^+$ but could not prevent its increase. Our results indicate that this polychaete is helpful in the recovery of seawater and sediment from eutrophication.

Protein Removal by a Foam Fractionator in Simulated Seawater Aquaculture System

  • Peng, Lei;Oh, Sung-Yong;Jo, Jae-Yoon
    • Ocean and Polar Research
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    • v.25 no.3
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    • pp.269-275
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    • 2003
  • Effects of different operating factors including superficial air velocity (SAV), hydraulic residence time (HRT), protein concentration, and foam overflow height on protein removal by a foam fractionator in simulated seawater aquaculture system were investigated. This experiment was conducted on batch and consecutive modes at different combinations of the affecting factors. The foam fractionator had a diameter of 20cm and a height of 120cm and the experiment was conducted with synthetic wastewater. In 5 consecutive trials, protein concentrations in culture tank water decreased faster when the foam fractionator was operated at higher SAVs and lower HRTs. In batch trials, protein removal rates increased with an increase in SAV but decreased with an increase in URT. Higher protein concentrations in the bulk solution resulted in higher protein removal rates. Protein concentrations in the collected foam condensates increased but the foam overflow rates decreased with the increase of foam overflow heights. The results of this experiment indicate that foam fractionation would be an effective way for protein removal in seawater aquaculture systems and the performance of the foam fractionator depends largely on the operating parameters, especially SAV.

Performance of a Foam Fractionator in a Lab-scale Seawater Recirculating Aquaculture System

  • Peng Lei;Jo Jae-Yoon
    • Fisheries and Aquatic Sciences
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    • v.6 no.4
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    • pp.187-193
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    • 2003
  • The performance of a foam fractionator to remove TAN, $NO_2,\;NO_3$, TSS, protein, and $PO_4-P$ at different superficial air velocities and foam overflow heights was evaluated in a lab-scale seawater recirculating system for culture of Korean rockfish (Sebastes schlegeli). The foam overflow rates increased with the increase of superficial air velocities, but decreased with the increase of foam overflow heights. Concentrations of all the water quality variables in the foam condensates increased with the increase of foam overflow height, but decreased with the increase of superficial air velocities. TSS, protein, and phosphate enrichment factors were within the range of 6.4-39.4, 1.6-7.3 and 1.2-3.9, respectively. Low values of TAN, $NO_2,\;and\;NO_3$ enrichment factors were obtained and they indicate that foam fractionation is rot an effective way to remove dissolved inorganic nitrogen. The calculated maximum daily removal values for TSS and protein were 10.9 and 1.4g, respectively.

Simulation for High Efficient Heat Pump System using Seawater Heat Source and Exhaust Energy (해수 열원 및 폐열 이용 고성능 열펌프 시스템 모사)

  • 최광일;오종택;오후규
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.15 no.1
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    • pp.59-66
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    • 2003
  • The purpose of this study is to analyze the characteristics (COP) of the heat pump system for various operating conditions with the use of seawater heat source and exhaust energy. To accomplish the goal, first of all, the computer simulation for heat pump system is carried out. The heat pump system model is made of a waste heat recovery system and a vapor compression refrigeration system, and the working fluid is R-22. The model calculated the change of COP with the variation of temperature and flow rate. The COP and the plate heat exchanger (PHE) area of the heat pump system are considered moderately high in the condensation temperature of $25^{\circ}^C$ and the evaporation temperature of $2^{\circ}^C$ in indoor culture system. The simulation results will be used effectively for the design and the performance prediction of heat pump system using unused energy in a land base aquaculture system.

Distribution of Polycyclic Aromatic Hydrocarbons in Farmed Oysters (Crassostrea gigas) around Tongyeong, Korea

  • Cho, Sang-Man
    • The Korean Journal of Malacology
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    • v.26 no.2
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    • pp.107-114
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    • 2010
  • To evaluate the culture conditions in oyster-farming waters, chemical and biological measurements were made in seawater and oysters from six bays around Tongyeong in November and December 2003. Nutrient levels in the seawater were higher in the western area than in the eastern area, in contrast to particulate organic matter and dissolved oxygen levels. The mean total polycyclic aromatic hydrocarbon ({\sum}PAH) content of the oysters was 194.5-375.9 ng/g dry weight, with four-ring compounds constituting 34.1%-79.6% of PAH. Despite wide temporal variations, a "western > eastern" spatial distribution of PAH was apparent. These low concentrations of PAHs indicate that Tongyeong waters are pristine in terms of PAH contamination. Among the hemocytic biomarkers, only lysosomal activity was significantly reduced in Hansan-Goje Bay, but did not correlate closely with PAH content. This finding indicates that the impact of PAH on cultured oysters is negligible around Tongyeong waters.

Evaluation offish Flesh and Profitability of Black Porgy (Acanthopagrus schlegeli) Cultured in Freshwater (담수양식 감성돔(Acanthopagrus schlegeli)의 어육평가 및 수익성 분석)

  • Min, Byung-Hwa;Bang, In-Chul;Choi, Woon-Su;Chang, Young-Jin
    • Journal of Aquaculture
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    • v.19 no.1
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    • pp.14-18
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    • 2006
  • The objective of this study was to evaluate food value and profitability of black porgy (Acanthopagrus schlegeli) cultured in freshwater. In fish flesh, muscular hardness of black porgy reared in freshwater $(9210{\pm}1215g/cm^2)$ was slightly lower than those of fish in seawater $(9987{\pm}6549g/cm^2)$, but there was no significant difference. Also, there was no difference between muscular strength of fish reared in freshwater and seawater. When the flesh qualities of black porgy reared in freshwater was compared with those of fish reared in seawater through the questionnaire, there were no significant differences between fish reared in freshwater and seawater in appearance, texture, taste and flavor. For 10 months of black porgy culture in fresh water, the gross profit in culturing from juvenile (5.5 g) to adult size (100g), and from adult to marketable size (400 g) were 24,000,000 won (30.0%) and 53,870,000 won (36.9%), respectively.

Deep Seawater Increases Dendritic Branches of Cultured Rat Hippocampal Neurons (해양심층수에 의한 해마신경세포 가지돌기 수의 증가)

  • Lee, Hyun-Sook;Nam, Kyung-Soo;Shon, Yun-Hee;Moon, Il-Soo
    • Journal of Life Science
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    • v.18 no.6
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    • pp.897-901
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    • 2008
  • Deep seawater (DSW; deep ocean water) is pure, rich in inorganic materials which have attracted attention for various applications. In this study we investigated the effects of the DSW upwelled from the East Sea, offshore Yang Yang (Korea) on the morphological differentiation of cultured rat hippocampal neurons, which were grown in the minimal essential medium containing 10% (v/v) fetal bovine serum and 25% (v/v) DSW with various hardness. DSW had no effect on initial morphological differentiation (17 hr post-plating). When observed on DIV3, 7, 14, and 17, low hardness (0 and 200) DSW reduced dendritic branching. However, dendritic branches within $80\;{\mu}m$ diameter from the center of soma nearly doubled in neurons grown in hardness 1,000 DSW-containing media. DSW with hardness 600 was more or less same as control groups. These results indicate that DSW with appropriate hardness ameliorates neuronal health.

Cultural characterization of probiotic Lactobacillus sakei BK19

  • Yang , Byung Gyoo;Song , Choon Bok;Yeo , In Kyu;Lee , Kyoung Jun;Park , Geun Tae;Lee, Sang Hyeon;Son, Hong Joo;Heo, Moon Soo
    • Journal of fish pathology
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    • v.16 no.2
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    • pp.119-123
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    • 2003
  • We have selected an valuable pmbiotic strain; Lactobacillus sakei BK19 which has wide antagonic spectrum against fish pathogens . Present study investigated cultural characterization of L. sakei BK19 including pH tolerance , susceptibility of antibacterial agents and growth pattern with different environment such as nutritions, temperature and salinity. L. sakei BK19 showed Significantly higher resistance at low pH(around pH 4) environment and relative high antibiotic tolerance . In the study of optimal culture condition, maltose and saccharose provided the optimal nutritional culture condition while lactose and mannitol were unable to supply its carbon source for the fermentation of L. sakei BK19. Moreover. L. sakei BK19 showed good growth at the temperature range of 15 to $45^{o}C$ und the NaCl concentration of 0 to 7%. Hence, this particular probiotic strain may be benificial both in seawater and fresh weter conditions.