• KSBB Journal
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• 제31권2호
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• pp.113-119
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• 2016

A novel agar-degrading marine bacterium, SH-1 strain, was isolated from seashore of Namhae at Gyeongnam province, Korea. The SH-1 strain exhibited 98% similarity with Alteromonas species based on 16S rDNA sequencing and named as Alteromonas sp. SH-1. Alteromonas sp. SH-1 showed agarase activity of 348.3 U/L (1.67 U/mg protein). The molecular masses of the enzymes were predicted as about 85 kDa and 110 kDa by SDS-PAGE and zymogram. The enzymatic activity was optimal at $30^{\circ}C$ and the relative agarase activity was decreased as temperature increase from $30^{\circ}C$ and thus about 90% and 70% activities were shown at $40^{\circ}C$ and $50^{\circ}C$, respectively. The optimum pH was 6.0 for agarase activity in 20 mM Tris-HCl buffer and activities were less than 70% and 85% activity at pH 5.0 and pH 7.0, respectively, compared with that at pH 6. Agarase activity has remained over 90% at $20^{\circ}C$ after 1.5 hour exposure at this temperature. However, its activity was less than 60% at $30^{\circ}C$ after 0.5 h exposure at this temperature. The enzymes produced agarooligosaccharides such as agaropentaose and agarotriose from agarose, indicating that the agarases are ${\alpha}$-agarases. Thus, Alteromonas sp. SH-1 and its agarases would be useful for the industrial production of agarooligosaccharides which are known as having anticancer and antioxidation activities.

• Archives of Plastic Surgery
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• 제34권3호
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• pp.319-324
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• 2007

Purpose: Necrotizing fasciitis is a rapid progressive, life-threatening disease. Many organisms have been identified for causative pathogens of necrotizing fasciitis. And necrotizing fasciitis caused by Vibrio vulnificus is a matter of grace concern in Southwest seashore of Korea recently. But concrete analysis between these pathogens was not executed yet. Methods: Sixty necrotizing fasciitis patients were included in this study. We divided the patients into 2 groups: Group A was the case which Vibrio vulnificus was cultured for causative pathogen of necrotizing fasciitis, and Group B was the case of other organisms. And we analysed each group for treatment, progression and prognosis. Results: There was no significant difference between two groups in total hospital stay but there was a great difference in ICU stay and progression to septic shock. Also, there was a great difference in mortality within 48 hours but there was no difference in mortality of 48 hours after hospital visit. Conclusion: This indicates that intial management of necrotizing fasciitis cased by Vibrio vulnificus is the key of treatment. So initial medical management with early surgical intervention is necessary for necrotizing fasciitis.

• 환경생물
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• 제18권4호
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• pp.411-424
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• 2000

연안생태계의 호염성 톡토기와 개미군집의 생물다양성정도와 변동에 대해 알아보기 위해, 주변환경과 식생이 다른 세지역에서 정량채집을 실시하였다. 종조성이나 분포에 영향을 주는 요인을 찾기 위해 pH, 염도 및 전기전도도를 측정하였다. 연구결과 톡토기의 종조성 이나 분포에는 pH와 식생보다도 토성과 염도가 더 큰 영향을 주는 것으로 나타났다. 그러나 개미의 경우는 집을 지을 적당한 장소를 제공해 줄 수 있는 식생과 조수에 의한 침수에 더 큰 영향을 받는 것으로 사료된다. 모든 조사지역에서 조상대가 조간대보다 다양도가 높았으며 조간대중에서는 모래함량이 높은 동호의 상조대에서 가장 높게 나타났다

• 농촌계획
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• 제19권1호
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• pp.23-32
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• 2013

In the traditional subsistence farming era in Korea, most of small farmer's housing plots in the rural villages had their boundary barriers of which materials being the natural ones provided from nearby places. However, during the rapid industrialization period from 1960s, a traditional type of boundary barriers in the rural villages had been replaced mostly by low-cost factory-made ones, which means absolute loss of their amenity values as a linear part of villagescape. In spite of many study efforts on boundary barriers of traditional rural houses, detailed historical dating on them after 1960s have not tried up to now, which provoked to try this study. Through the direct and in-depth interviews to rural villagers sampled from 21 case villages, it was found that original boundary barriers structure in most of farm houses was made of natural materials like stone, soil, trees and bamboo, but, replaced by cement block structures mostly during Saemaeul Project period. However, although being relatively minority, traditional ones have been preserved up to now mainly in remote mountainous and seashore villages, but was in very low condition. In the overall view, for more than half a century, boundary barriers of farm houses had been left without public or private improvement efforts. In order to enhance their amenity values, more research works should be carried out on positive reassessment of the traditional ones harmonized with multifunctionalities for modern village life.

• Weed & Turfgrass Science
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• 제4권1호
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• pp.65-70
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• 2015

영국갯끈풀(Spartina anglica)은 영국 남부해안 원산의 다년생 염생 잡초로, 2012년 강화도 동막 해변에서 분포가 최초로 확인되었다. 이에 분포 및 확산실태를 보고하며 식별을 위한 식물학적 특성을 제시하였다. Spartina 속은 중국을 포함한 세계 여러 나라에 도입되어 생태공학적 측면의 장점이 인정되었으나, 갯벌을 단일 초지화시키면서 염습지의 생태적 기능을 상실시키는 침입성이 강한 식물로 알려졌고, 제거가 쉽지 않아 관리에 어려움을 겪고 있다. 한국의 서해안 갯벌에서 출현한 영국갯끈풀의 확산은 국내 갯벌 생태계에 심각한 위협을 초래할 수 있으므로 적극적인 제거방안 수립과 지속적인 관찰이 필요하다.

• 한국작물학회지
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• 제54권1호
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• pp.119-123
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• 2009

최근 활발한 발굴에 따른 곡물의 출토가 많아짐에 따라, 벼의 한반도(韓半島) 전래(傳來) 경로(經路)에 대한 재검토가 필요하다고 생각된다. 특히 한반도(韓半島) 최고(最古) 벼인 청원 소로리(小魯里) 벼의 전래경로에 대하여 제4기 자연환경(自然環境), 생태적(生態的), 작물학적(作物學的)면을 검토한 결과는 다음과 같다. 1. 소로리(小魯里) 볍씨는, 최대빙하기(最大氷河期)이후 한반도가 중국대륙과 하나의 대륙으로 연결되었을 당시 한국조상(韓國祖上) 중 남방계(南方系)의 한 부류가 볍씨를 가지고 중국대륙 남부지역에서 해안을 따라 동북진(東北進)하다가 당시 옛 금강하류(錦江下流)를 거쳐 소로리(小魯里)로 이동하였다고 추정된다. 이를 "옛 금강-소로리 볏길(Old Geumgang-Sorori Rice Road)"이라 명명한다. 2. 유전적(遺傳的) 변이(變異)가 큰 소로리(小魯里)벼는 빙하기이후 지리적(地理的), 생식적(生殖的) 격리(隔離)가 발생함에 따라 한반도(韓半島)의 자연환경(自然環境)에 적응(適應)(자연선택(自然選擇))하게 되어 변이가 적은 고대(古代)벼(가와지(家瓦地)벼)로 종(種)의 분화(分化)(논벼, 수도(水稻))가 이루어졌다고 추정된다. 즉 한반도에서 순화단계(馴化段階)인 소로리(小魯里)벼가 재배(栽培)벼로 발전(發展)하였다고 판단된다.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 하계학술발표대회 논문집
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• pp.1353-1357
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• 2008

A sea water source cascade heat pump was designed and tested in this study. The system was designed to perform a single stage operation in summer, as well as a cascade operation in winter to ensure the high temperature lift. A steady-state simulation model was developed to analyze and optimize its performance. The simulation results show that the R717 exhibits best performance among combinations considered in this study. A R410A also exhibits the highest performance among HFCs with the smallest compressor displacement. A 15-RT R410A-R134a pilot system was installed in the 5-story commercial building at Samcheok City by the East Sea. A scroll type R410A compressor, a reciprocating type R134a compressor, plate type condenser/ evaporator/ cascade heat exchanger and two electronic expansion valves were used to build a pilot. A titanium plate type heat exchanger is also used for the heat exchanging with a sea water. The heat source/sink water is supplied from the well below the seashore in the depth of 5 m. In the initial test of the system, supply water temperature was rising up to $67^{\circ}C$ using a sea water heat source of $9^{\circ}C$, while an ambient temperature was $4.5^{\circ}C$.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 하계학술발표대회 논문집
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• pp.1273-1278
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• 2008

A sea water source cascade heat pump was designed and tested in this study. The system was designed to perform a single stage operation in summer, as well as a cascade operation in winter to ensure the high temperature lift. A steady-state simulation model was developed to analyze and optimize its performance. The simulation results show that the R717 exhibits best performance among combinations considered in this study. A R410A also exhibits the highest performance among HFCs with the smallest compressor displacement. A 15-RT R410A-R134a pilot system was installed in the 5-story commercial building at Samcheok City by the East Sea. A scroll type R410A compressor, a reciprocating type R134a compressor, plate type condenser/ evaporator/ cascade heat exchanger and two electronic expansion valves were used to build a pilot. A titanium plate type heat exchanger is also used for the heat exchanging with a sea water. The heat source/sink water is supplied from the well below the seashore in the depth of 5 m. In the initial test of the system, supply water temperature was rising up to $67^{\circ}C$ using a sea water heat source of $9^{\circ}C$, while an ambient temperature was $4.5^{\circ}C$.

• 한국환경생태학회지
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• 제24권2호
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• pp.93-100
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• 2010

태안해안국립공원은 우리나라 유일의 해안국립공원으로 대표적인 4개 섬인 외도, 대도, 장고도, 그리고 가의도에 대해 식생특성을 밝혔다. 각 섬마다 식생구조를 밝히고 비교를 하기 위하여 단위면적 $100mm^2$의 조사구 4개씩을 균등하게 설치하였다. 4개 섬에 대해 각각의 식생구조를 분석한 결과 외도와 장고도는 곰솔군락이 우점종이었으며, 고대도는 곰솔-소나무군락, 가의도는 소사나무-소나무군락이 우점종이었다. 그러나 각 섬의 물리적 환경에 따라 같은 곰솔이나 소나무군락이라도 종조성에 따라 차이가 있었다. Ordination과 유사도분석결과 외도와 가의도의 유사도가 6.58%로 가장 낮았으며, 종다양도(2.7183) 및 개체수 분석 결과 가의도가 가장 높은 것으로 나타났다.

• 생명과학회지
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• 제18권1호
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• pp.58-62
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• 2008

기능성 한천올리고당 생산에 사용할 수 있는 유전자원을 확보하기 위하여 동해안 기장 해수에서 한천분해활성을 보이는 해양유래 세균 SL-12를 분리하였으며 165 rDNA염기서열 분석으로 해양기원의 Glaciecola 속과 가장 유사한 균주임을 확인하였다. SL-12 균주가 생성하는 한천분해효소(agarase)의 최적 pH는 pH 7.0 (20 mM sodium phosphate 완충용액)이고 최적 온도는 $30^{\circ}C$로 나타났다. 분리 된 Glaciecola sp. SL-12 균주가 생산하는 한천분해효소의 분해산물에 대한 TLC 분석결과, 기능성 한천올리고당을 생산하는 ${\beta}$-agarase로 판명되어 산업적 활용 가능성이 높은 것으로 기대된다.