• Title/Summary/Keyword: Sea Sand

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Purification and Characterization of a Chitinase from Cytophaga sp. HJ Isolated from Sea Sand

  • Lee, Dong-Mi;Noh, Hee-Jung;Lee, Kang-Man
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.839-846
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    • 1999
  • An extracellular chitinase-producing bacterial strain induced by colloidal chitin was isolated from sea sand and was identified to be a member of the genus Cytophaga. The chitinase was purified successively by 30-60% ammonium sulfate fractionation, and DEAE-Bio gel A column, Octyl-Sepharose CL-4B column, and DEAE-Bio gel A column chromatographies. The enzyme had a molecular mass of 59.75 kDa, and the amino terminal amino acid sequence was ATPNAPVISW MPTDXXLQNXS. The enzyme acted better on colloidal chitin as a substrate than on chitosan. For colloidal chitin and chitosan (Degree of Acetylation, 15-25%), $K_{cat}$ values were 0.60U/mg and 0.08U/mg, respectively. HPLC analysis of the enzymatic reaction products showed that the chitinase produced mostly N-acetyl-D-glucosarnine and di-N-acetylchitobiose. The optimum temperature and pH for the enzyme were $50^{\circ}C$ and 4.0, respectively. N-Bromosuccinimide and $Hg^{2+}$ inhibited the chitinase activity as much as 90%, and $Sb^{3+}$, diethylpyrocarbonate, and $Ag^{+}$ inhibited it by 50-70%.

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An Electrochemical Study on the Effect of Salt Affecting to Corrosion Behavior of Concrete Reinforced Steel in Natural Sea Water (천연해수에 침지된 콘크리트 내부의 철근부식거동에 미치는 염분의 영향에 관한 전기화학적 연구)

  • 김광근;류보현;점성종;김기준;문경만
    • Journal of Ocean Engineering and Technology
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    • v.14 no.4
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    • pp.23-29
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    • 2000
  • When the concrete structures were being made with sand containing chloride ion it was knows that corrosion rate of reinforced steel embedded in concrete with chloride ion was higher than that of concrete with on chloride ion. In this study, the operation of Friedel salts affecting the corrosion behavior of reinforced steel embedded in cement mortar was investigated with electrochemical view. Corrosion potential of reinforced steel embedded in cement mortar with sand containing chloride ion was shifted noble direction than that of cement mortar with no chloride ion after immersed 5 month in natural sea water and also corrosion current density decreased with shifting corrosion potential to noble direction. However Friedel salts appeared from surface to 2.5cm of inside direction of mortar specimen, which is located at 11.5$\circ$(2$\theta$) in XRD analysis and the amount of Ca(OH)2 by SEM photograph in cement mortar with chloride ion was larger than that of cement mortar with mo chloride ion. Eventually it is suggested that Friedel salts was resulted from chloride ion and it acted as the corrosion inhibitor.

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Draft genome sequence of Miniimonas arenae KCTC 19750T isolated from sea sand (해양모래로부터 분리된 Miniimonas arenae KCTC 19750T의 유전체 분석)

  • Park, Soo-Je
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.278-279
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    • 2019
  • Miniimonas arenae KCTC $19750^T$ belonging to family Beutenbergiaceae of the phylum Actinobacteria was isolated from sea sand. I report here the draft genome sequence of strain KCTC $19750^T$. The draft genome comprises a size of 3,402,690 bp, a mean G + C content of 73.6%, 2,957 coding sequences, 2 ribosomal RNA genes, and 44 transfer RNA genes. Also, we found that genes involved in osmotic stress response were identified in its genome. The availability of the genome sequences will provide a more understanding of strain KCTC $19750^T$ as a unique member of the genus Miniimonas.

Origin and Characteristics of Sand Ridges in the western Continental Shelf of Korean Peninsula (한반도 서부대륙붕에 발달한 사퇴의 발생기원과 특성)

  • 방효기;이차원
    • 한국해양학회지
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    • v.29 no.3
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    • pp.217-227
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    • 1994
  • From northeast to southwest, discontinuous sand ridges distribute on the western continental shelf of Korean Peninsular. The dimension of sand ridges is 3 to 21 m high, 3.1 to 6.8 km wavelength and 9-64 km long with 0.5 steep slope. they are probably originated and reformed by the intensity of tidal current according to the sea level rise. The characteristics of sand ridges revealed in study area are summarized as follows: (1) The sand ridges line up with the long axes of the tidal current ellipses, indicating a tidal control. (2) these are composed of two sedimentary sequences on the 3.5 kHz seismic profiles and core sediments. The upper sequence characterized by prolonged type is covered with thin veneer of massive fine sand(Mz, 2-3$\phi$) with Olive Gray(5Y 5/2). The lower sequence is characterized by internal reflector type with parallel and discontinuous. It consists of sandy mud or muddy sand(Mz, 5-7$\phi$) with laminar structures. the parallel internal reflectors are truncated on the slope of sand ridges. (3) Asymmetrical sand waves are superimposed on the sand ridges, and facing to the crest. However, symmetrical sand waves lie on the crest. Sand ridges having characteristics above is originated by scouring of tidal current, covered with coarase relict sediments, and modified by sadware.

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Textural Characters of the Sediments from Neolithic site Moonamni Coastal Zone, East Sea of Korea -Implication of the Holocene High Stand Sea Level (강원도 동해안 문암리 신석기 유적지 퇴적층의 조직 특성)

  • 박용안;김수정;최진용
    • The Korean Journal of Quaternary Research
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    • v.17 no.1
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    • pp.27-37
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    • 2003
  • The Neolithic relics containing sedimentary deposits have been found in the Moonamni coastal zone of the East Sea, Korea. The purpose of this research is to establish the late Quaternary stratigraphy of the coastal dune deposit and to elucidate its depositional environment of the Neolithic-site sediments on the basis of analytical properties of grain size population and mineralogy of the sediments. As a result, the vertical sections of the sediments from three trenches are characterized by three major stratigraphic depositional units of Unit 3, Unit 2 and Unit 1 in ascending order. Unit 3 and 2 can be further divided into tow sub-units. Unit 3 is composed of massive sands in the lower part and muddy sand in the upper part. It is considered that the Unit 3 is a typical dune deposit showing well-sorted sands. Unit 2 is characterized by the cross-bedding, and include archaeological remains such as pottery shards. This unit can be further divided into two sub-units of muddy sand in the lower part and sand in the upper part. Unit 1 occupies the top section and consists of modem dune sediment. The Neolithic cultural remains would be accumulated in the coastal dune area in relation to dynamic condition of beach system under the high stand of Holocene sea-level at about 7,800∼6,500 yr B.P. or so.

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Factors affecting particle breakage of calcareous soil retrieved from South China Sea

  • Wang, Xinzhi;Shan, Huagang;Wu, Yang;Meng, Qingshan;Zhu, Changqi
    • Geomechanics and Engineering
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    • v.22 no.2
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    • pp.173-185
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    • 2020
  • Calcareous soil is originated from marine biogenic sediments and weathering of carbonate rocks. The formation history for calcareous sediment includes complex physical, biological and chemical processes. It is preferably selected as the major fill materials for hydraulic reclamation and artificial island construction. Calcareous sands possess inter pores and complex shape are liable to be damaged at normal working stress level due to its fragile nature. Thus, the engineering properties of calcareous soil are greatly affected by its high compressibility and crushability. A series of triaxial shear tests were performed on calcareous sands derived from South China Sea under different test conditions. The effects of confining pressure, particle size, grading, compactness, drainage condition, and water content on the total amount of particle breakage for calcareous soil were symmetrically investigated. The test results showed that the crushing extent of calcareous sand with full gradation was smaller than that a single particle group under the same test condition. Large grains are cushioned by surrounding small particles and such micro-structure reduces the probability of breakage for well-graded sands. The increasing tendency of particle crushing for calcareous sand with a rise in confining pressure and compactness is confirmed. It is also evident that a rise in water content enhances the amount of particle breakage for calcareous sand. However, varying tendency of particle breakage with grain size is still controversial and requires further examination.

Population Ecological Characteristics of the Soft-shelled Clam, Mya japonica in the Intertidal Zone of South Sea in Korea (한국 남해안 조간대에 서식하는 우럭, Mya japonica의 자원생태학적 특성)

  • 이선길;장창익
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.36 no.3
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    • pp.234-243
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    • 2000
  • This paper is to study population ecological characteristics, including growth parameters, survival rate, instantaneous coefficients of natural and fishing mortalities, and age at first capture of the soft-shelled clam, Mya japonioa in the intertidal zone of South Sea in Korea. For describing growth of the clam a von Bertalanffy growth model was adopted, The von Bertalanffy growth curve had an additive error structure and the growth parameters estimated from a non-linear regression were SH/sub ∞/=79.83mm, K=0.26, and t/sub 0/= -0.01. Survival rate (S) of the soft-shelled clam was 0.26 (SD=0.02). The instantaneous coefficients of natural mortality (M) was estimated to be 0.78/year and fishing mortality (F) 0.57/year for the soft-shelled clam. The age at first capture (t/sub c/) was estimated as 2.69 year. The mean densities of the soft-shelled clam by bottom type were 3.40 inds./m²(SE=0.18) in the sand, 63.4 inds./m²(SE= 0.53) in the muddy sand, and 0 inds./m2 (SE=0) in the gravelly sand. The mean densities of the soft-shelled clam by 3 different areas were 4.88 inds./m²(SE=0.09), 2.61 inds./m²(SE=0.13), 7.20 inds./m²(SE=0.18), respectively and the biomass of the clam were estimated as 131mt, 121mt, 665mt, respectively. An yield-per-recruit analysis showed that the current yield-per-recruit of about 8.30g with F=0.57/year and the age at first capture (t/sub c/) 2.69 year, was lower than the maximum possible yield-per-recruit of 9.60g. Fixing to at the current level and increased fishing intensity (F) could produce an increase in the predicted yield-per-recruit from 8.30g to about 9.40. However, estimated yield-per-recruit increased to 1.30g by decreasing to from the current age (2.69 year) to age two with F fixed at the current level. Yield-per-recruit was estimated under harvest strategies based on F/sub max/ and F/sub 0.1/.

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Analysis of marine sediments between fishing area and non-fishing area in the shrimp beam trawl (새우조망 조업구역과 비조업구역의 해저퇴적물 분석)

  • Cho, Sam-Kwang;Yang, Yong-Soo;Cha, Bong-Jin;Seo, Young-Kyo
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.48 no.3
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    • pp.208-216
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    • 2012
  • The properties of sediment collected from seabed surface to 6cm depth on the four positions were analyzed to investigate turbulence of marine sediments by shrimp beam trawl. Types of sediments in the investigation area were (g)mS (slightly gravely muddy sand) and gmS (gravely muddy sand) showing high sand content, and (g)sM (slightly gravely sandy mud), gsM (gravely sandy mud) as well. It is estimated that position is more crucial factor than seasonal difference for the granularity variation of sediment in each investigation area. Finding the positional characteristics of sediment granularity was difficult before removing shells and organic matter. However, the average granularity is getting larger by going out from inland sea to open sea once those were removed. The granularity of marine sediment got narrow after processing in the fishing area for shrimp beam trawl but there was no big difference for granularity size before and after processing in the non-fishing area. This might be attributed to crushed shell particles going up and down again on the surface in the fishing area. To demonstrate the hypothesis mentioned above, the sediments driven by shrimp beam trawl need to be collected and analyzed.

EFFECT OF SILT AND SALINITY ON THE MORTALITY OF MERETRIX LUSORIA $(R\ddot{O}DING)$ (이질과 염분이 백합의 폐사에 미치는 영향)

  • CHANG Sun-duck;CHIN Pyung;SUNG Byung-oun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.1
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    • pp.69-73
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    • 1976
  • Mortality of clam, Meretrix lusoria was measured during the experimental cultures in different sediments compositions and in different salinities of sea water. Experimental groups maintained in silt and clay showed significant mortality, while those treated in sand and in sand-silt showed little mortality. In the groups cultured in silt and clay, young groups of approximately 2.5cm in shell length were subjected to early and high mortality (approximately eighty-five per cent in foully-one days). The larger the shell size was, the later and the lower the mortality occurred. In different salinity, the groups maintained in silt and clay showed different mortalities. It is observed that the survival rate of the shell in the sea water of low salinity was higher than that in the high salinity water. Oxygen consumption of isolated gill tissue showed a little difference between the groups maintained in silt and clay and those in sand (the control-group). Consequently, it may be stated that the mortality results mainly from the deposition of silt and clay, although the survival rate of Meretrix lusoria depends also on water temperature, salinity, dissolved oxygen, body size ana other factors.

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Growth of Two Native Zoysiagrasses Collected from Sea Side and Mountain Area in Indonesia on Growing Media Composed of Sand and Clay

  • Rahayu, Rahayu;Dewantoro, Hery;Arianto, Dwi Priyo;Bae, Eun-Ji;Choi, Su-Min;Lee, Kwang-Soo;Yang, Geun-Mo;Choi, Joon-Soo
    • Weed & Turfgrass Science
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    • v.7 no.1
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    • pp.54-61
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    • 2018
  • Zoysiagrass (Zoysia spp.) exists spotly in Indonesia and it has potential to be used in parks, golf courses, and football fields. Many football fields and golf course fairways use sand as top soil over native soil. This study aims to analyze growth and quality of two native zoysiagrasses Zis and Zim. Zis is a native zoysiagrass collected in sea-side and Zim is a native zoysiagrass collected in mountain area. Both types of zoysiagrasses were planted at field with altitude of 300 m with various growing media mixes of sand and reservoir's sediment. Thickness of the growing medium was 10 cm over an alfisol clay soil. Experimental plots were constructed using factorial completely randomized design with two native zoysiagrasses and 5 types of growing media. Two ecotypes of native zoysiagrasses showed different in growth habits combined with mixtures of growth media. Zim showed higher growing speed including more vigor with uniformity and texture than Zis. There were not significanthly differences on leaf color and root length between two ecotypes of native zoysiagrasses collected in Indonesia.