• Title/Summary/Keyword: Sdu1

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Defensive roles of Sdu1, a PPPDE superfamily member with ubiquitin C-terminal hydrolase activity, against thermal stress in Schizosaccharomyces pombe (카르복시 말단 유비퀴틴 가수분해 효소 활성 보유 PPPDE superfamily member인 Schizosaccharomyces pombe Sdu1의 열 스트레스에 대한 방어적 역할)

  • Han, Hee;Heo, Tae Young;Ryu, In Wang;Kim, Kyunghoon;Lim, Chang-Jin
    • Korean Journal of Microbiology
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    • v.51 no.4
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    • pp.319-328
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    • 2015
  • The $sdu1^+$ gene encodes Sdu1, a PPPDE superfamily member of deubiquitinating enzymes (DUBs) in Schizosaccharomyces pombe. Sdu1 was previously shown to contain an actual ubiquitin C-terminal hydrolase (UCH) activity using the recombinant plasmid pYSTP which harbors the $sdu1^+$ gene. This work was designed to assess a thermotolerant role of Sdu1 against high incubation temperatures. In the temperature-shift experiments, the S. pombe cells harboring pYSTP grew much better after the shifts to $37^{\circ}C$ and $42^{\circ}C$, when compared with the vector control cells. After being shifted to $37^{\circ}C$ and $42^{\circ}C$ for 6 h, the S. pombe cells harboring pYSTP contained lower reactive oxygen species (ROS) levels, compared with the vector control cells. The nitric oxide (NO) levels of the S. pombe cells harboring pYSTP were slightly lower than those of the vector control cells in the absence or presence of the temperature shifting. The total glutathione (GSH) levels of the S. pombe cells harboring pYSTP were significantly higher than those of the vector control cells. Total superoxide dismutase (SOD) and GSH peroxidase activities were also higher in the S. pombe cells harboring pYSTP after the temperature shifts than in the vector control cells. In brief, the S. pombe Sdu1 plays a thermotolerant role against high incubation temperature through the down-regulation of ROS and NO and the up-regulation of total GSH content, total SOD and GSH peroxidase activities.

Reactive oxygen species-dependent down-regulation of ubiquitin C-terminal hydrolase in Schizosaccharomyces pombe (Schizosaccharomyces pombe에서의 유비퀴틴 C-말단 가수분해효소의 활성산소종 의존성 하향조절)

  • Jo, Hannah;Lim, Hye-Won;Kwon, Hee-Souk;Lim, Chang-Jin;Park, Kwang Hark;Jin, Chang Duck;Kim, Kyunghoon
    • Korean Journal of Microbiology
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    • v.52 no.2
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    • pp.236-241
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    • 2016
  • The Schizosaccharomyces pombe $sdu1^+$ gene, belonging to the PPPDE superfamily of deubiquitinating enzyme (DUB) genes, was previously shown to encode a protein with ubiquitin C-terminal hydrolase (UCH) activity and to participate in the response against oxidative and nitrosative stresses. This work focused on the reactive oxygen species (ROS)-dependent regulation of the S. pombe $sdu1^+$ gene. UCH activities, encoded by the $sdu1^+$ gene, were attenuated in the S. pombe cells exposed to $H_2O_2$, superoxide radical-generating menadione (MD), and nitric oxide (NO)-generating sodium nitroprusside (SNP). Reduced glutathione (GSH) and its precursor N-acetylcysteine (NAC) were able to significantly enhance the UCH activities in the absence or presence of $H_2O_2$. However, the influences of both GSH and NAC on the ROS levels in the absence or presence of $H_2O_2$ were opposite to their effects on the UCH activities under the same conditions. The UCH activities in the Sdu1-overexpressing S. pombe cells were also diminished under exposure to $H_2O_2$, MD and SNP, but still remained to be higher than those in the vector control cells. In brief, it is proposed that the S. pombe $sdu1^+$ gene is regulated by ROS in a negative manner, the meaning of which largely remains elusive.

Mechanistic Studies on the Anormalous Photocycloaddition Reaction of 5-Styryl-1,3-dimethyluracil and 2,3-Dimethyl-2-butene: Formation of the “Apparent Forbidden” [$_\pi4_s + _\pi2_s$] Cycloadduct

  • Eun Ju Shin;Ho Kwon Kang;Sang Chul Shim
    • Bulletin of the Korean Chemical Society
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    • v.12 no.4
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    • pp.434-437
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    • 1991
  • Irradiation of 5-styryl-1,3-dimethyluracil (5-SDU) with 2,3-dimethyl-2-butene (DMB) gives a [4+2] cycloadduct which is converted into a [2+2] cycloadduct on the prolonged irradiation. Triplet sensitization, quenching, and external heavy atom effect on the [4+2] photocycloaddition reaction demonstrate the singlet pathway and salt effect excludes a radical ion pair precursor possibility. Polar solvents increase the reaction efficiency implying a polar exciplex involvement in the [4+2] photocycloaddition reaction. Inverse temperature dependence both on the reaction and DMB fluorescence quenching of 5-SDU indicates the presence of a singlet exciplex intermediate.

A Study on the Design and Validation Methodology of Communication Protocols Using International Communication Standard Languages (국제 통신 표준 언어를 이용한 통신 프로토콜 설계 및 검증 방법론 연구)

  • Ro, Cheul-Woo
    • The Journal of Korean Association of Computer Education
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    • v.5 no.4
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    • pp.31-42
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    • 2002
  • In this paper, We set up the development methodology of communication protocols as well as the concrete design concept concerning for how to define and use the PDU, SDU, SAP, and service primitives using SDL, which is recommended by ITU-T, and other international standard languages such as ASN.l, MSC, and TTCN. This methodology covers the SDL design of extended lures protocol, a well known protocol example, insertion of ASN.1 message for transportation of bit string, generation of MSC for validation of design specification, generation of test cases using TTCN from validation, and performance of conformance test.

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원격교육솔루션의 동향과 전망

  • 성기영
    • Proceedings of the Korea Society for Industrial Systems Conference
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    • 2000.11a
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    • pp.69-84
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    • 2000
  • 교육 시장의 변화 ; 1. 평생 교육법 및 시행령 공포(1995. 9. 5. 평생교육법 선포, 2000. 3. 13 평생교육법 시행령 공포, 200. 3. 31 평생교육법 시행 규칙 제정), 2. 교육 시장의 변화 과외금지 위헌 판결; 30조원 사교육 시장 부상, 온라인 가상 대학 증가 ; 대학 컨소시엄(OCU, KCU, KDU, SDU 등), 가상대학(16개교 81개 과 설립 신청), 직장 내 가상대학(삼성 반도체 공과대학 2개학과).(중략)

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The Marriage Stewardship and the Role and Status of Social Development Network in Singapore (결혼 책임주의와 싱가포르 Social Development Network의 역할과 위상)

  • Kim, Myung-Hee
    • Journal of the Korea Convergence Society
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    • v.9 no.1
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    • pp.143-149
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    • 2018
  • This paper focuses on how Singapore has adopted the 'Marriage Promotion Policy' for resolving the low birthrate and how it has been using the Social Development Network (SDN) to cope with the problem of low fertility. Social Development Network is an organization that shows Singapore's national stewardship to marriage, and has developed from the beginning to the social phenomenon of increasing number of highly educated women and decreasing fertility. The predecessor of Social Development Network is 'Social Development Unit' (SDU), absorbed the 'Social Development Service' (SDS) that was launched later, and reborn as Social Development Network in 2009. At its inception, Social Development Network has been recruiting and organizing its own memberships without the help of commercial marriage agencies, and since 2006 has been dedicated to delegating dating and matchmaking services to private companies and supporting projects. The purpose of this paper using literature analysis is to analyze the status and role of Social Development Network from the theoretical point of view of marital stewardship to the present, and to suggest meaningful implications for Korea which has the same social experience.

Improving hydration in elite male footballers during a national team training camp - an observational case study

  • Mohr, Magni;Nolsoe, Eli Leifsson;Krustrup, Peter;Fatouros, Ioannis G.;Jamurtas, Athanasios Z.
    • Korean Journal of Exercise Nutrition
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    • v.25 no.4
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    • pp.10-16
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    • 2021
  • [Purpose] The purpose of this study was to (i) assess hydration levels in elite male football players during a national team training camp before and during qualifying matches, (ii) evaluate the effect of coaching strategies for hydration based on feedback from hydration monitoring, and (iii) assess possible relationships between hydration status and training load or wellness markers. [Methods] Thirty-one male players (age 27±4 yrs; height 185±6 cm; weight 82.9±6.7 kg; body fat 10.4±2.3%) representing a national team from the Union of European Football Associations (UEFA) participated. The players were studied during three different national team training camps related to the UEFA Nations League tournament. Urine specific gravity (USG) was measured to assess hydration status. During all camps, the players were actively coached on improving strategies for hydration and given individual feedback on their test results. The training load was measured using GPS technology, and wellness questionnaires were completed. [Results] USG decreased progressively and significantly (p<0.005) during camp 1 and hydration status improved over the three camps, with fewer dehydrated and more well-hydrated players identified during the last part of camp 3. Significantly (p<0.05) higher USG values were observed 2 days prior to a match (MD-2) than on match day (MD); consequently, 52% of the players were dehydrated on MD-2 and only 6% on MD. No correlations were observed between hydration status and training load or wellness markers. [Conclusion] Dehydration is a challenge in elite male football, but continuous monitoring of hydration status and coaching on hydration strategies can lead to major improvements and reduce the degree of dehydration.

Throughput rate of DCF Protocol based Ricean fading channel in the IEEE 802.1la wireless LAN. (라이시안 페이딩 채널환경에서 IEEE 802.11a 무선 LAN의 DCF 처리율)

  • Ha Eun-Sil;Jung Jin-Wook;Lee Ha-Chul
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.9A
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    • pp.803-813
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    • 2005
  • This paper explores the throughput performance of CSMA/CA-based DCF protocol over frequency-selective, slow Ricean fading channels with both the $E_{b}/N_{o}$ and BER at the MAC layer in the 802.1 la wireless LAN. By exploring the throughput of DCF protocol with the data rate of 6Mbps, 12 Mbps, 24 Mbps and 54 Mbps, we find the fact that the higher the $E_{b}/N_{o}$ be and the less the BER be, the higher the throughput be.

Skin Disk Diffusion Method(SDU) as an Effective in vitro method for measurement of Anti-dandruff activity (효과적 in vitro 항비듬 측정법으로서의 Skin Disk Diffusion Method(SDDM))

  • 서경희;신계호
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.24 no.1
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    • pp.100-112
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    • 1998
  • 향 비듬 샴푸에 대한 비중이 증가되는 현 샴푸시장에서 향 비듬력을 측정할 수 있는 간단한 in vitro 실험법의 요구가 증대되고 있다. 기존의 향 비듬력의 측정을 위하여 사용되고 있는 방법으로는 Pityrosporum ovale에 대한 MIC test와 halo test가 널리 쓰이고 있으나, Pityrosporum 배지의 자체 탁도 때문에 균의 성장 여부를 육안으로 관찰하기 어렵고, 종이 디스크와 두피와의 차이에 의하여 실제 사용과 효과가 다르다는 단점이 있었다. 저자들은 임상 실험을 거치기 전 간단히 향 비듬력을 측정할 수 있는 in vitro 방법으로 skin disk diffusion method를 개발하였다. SDDM에서는 종이 디스크 대신 기니픽의 피부 조직을 사용하고, 향 비듬 제제의 처리 후 세척 단계를 포함시켜 실제 사용과의 차이를 줄였다. SDDM의 유용성을 확인하기 위하여 향 비듬 제제로 2.0% ketoconazole, 분산된 상태의 zinc pyrithion, 분자화 기술로 활성을 높인 ZPT에 대해 기존의 in vitro 시험법과 함께 향 비듬력을 측정하였다. 결과는 SDDM 시험에서만 20% ketoconazole 제제와 분자화된 0.5% ZPT제제의 억제대가 비슷한 것으로 나타나 임상 시험에서 두 제제 모두 비듬에 대해 개선 효과가 있었으며 향 비듬 효과에 차이가 없다는 결과와 일치하였다. 본 시험의 결과로 SDDM이 향 비듬 샴푸와 같은 wash-off 제품의 효능 측정에 간단하고 효과적인 적절한 in vitro 시험법으로 생각되어 SDDM 시험과 임상 시험결과를 소개한다.

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