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http://dx.doi.org/10.7845/kjm.2015.5042

Defensive roles of Sdu1, a PPPDE superfamily member with ubiquitin C-terminal hydrolase activity, against thermal stress in Schizosaccharomyces pombe  

Han, Hee (Department of Biochemistry, Kangwon National University)
Heo, Tae Young (Department of Biochemistry, Kangwon National University)
Ryu, In Wang (Department of Biological Sciences, Kangwon National University)
Kim, Kyunghoon (Department of Biological Sciences, Kangwon National University)
Lim, Chang-Jin (Department of Biochemistry, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.51, no.4, 2015 , pp. 319-328 More about this Journal
Abstract
The $sdu1^+$ gene encodes Sdu1, a PPPDE superfamily member of deubiquitinating enzymes (DUBs) in Schizosaccharomyces pombe. Sdu1 was previously shown to contain an actual ubiquitin C-terminal hydrolase (UCH) activity using the recombinant plasmid pYSTP which harbors the $sdu1^+$ gene. This work was designed to assess a thermotolerant role of Sdu1 against high incubation temperatures. In the temperature-shift experiments, the S. pombe cells harboring pYSTP grew much better after the shifts to $37^{\circ}C$ and $42^{\circ}C$, when compared with the vector control cells. After being shifted to $37^{\circ}C$ and $42^{\circ}C$ for 6 h, the S. pombe cells harboring pYSTP contained lower reactive oxygen species (ROS) levels, compared with the vector control cells. The nitric oxide (NO) levels of the S. pombe cells harboring pYSTP were slightly lower than those of the vector control cells in the absence or presence of the temperature shifting. The total glutathione (GSH) levels of the S. pombe cells harboring pYSTP were significantly higher than those of the vector control cells. Total superoxide dismutase (SOD) and GSH peroxidase activities were also higher in the S. pombe cells harboring pYSTP after the temperature shifts than in the vector control cells. In brief, the S. pombe Sdu1 plays a thermotolerant role against high incubation temperature through the down-regulation of ROS and NO and the up-regulation of total GSH content, total SOD and GSH peroxidase activities.
Keywords
Schizosaccharomyces pombe; glutathione; reactive oxygen species; Sdu1; thermal stress;
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