• 생약학회지
• /
• 제41권4호
• /
• pp.303-307
• /
• 2010

To overcome the limit of morphological method for classification of herbal drug, a novel method to discriminate its origin using pyrolysis mass spectrometry-multivariate analysis was developed. This method was applied successfully to Scutellaria baicalensis Georgi, one of the most popular herbal drug in oriental countries. The ethylacetate soluble fractions were prepared by sonication from pulverized roots of S. baicalensis which were collected from various regions including Korea and China, and subjected to direct insertion probe (DIP) mass spectrometry to achieve mass spectra of pyrolizates of extracts. The probe temperature was elevated from $30^{\circ}C$ to $320^{\circ}C$ at increasing rate $64^{\circ}C/min$, and the average mass spectrum calculated from total ion chromatography (TIC) was obtained. The relative peak intensities versus m/z were subjected to SAS program, and the training set (9 from Korea origin and 22 from China origin) was clustered two groups as its origin. In the test set, 11 samples among total 13 test sample were successfully classified according to their origin by developed method with accuracy of 85%.

• 약학회지
• /
• 제50권2호
• /
• pp.136-143
• /
• 2006

Roots of Scutellaria baicalensis have been used for fever remedy; diuresis, antiphlogistic. For the investigation of the active component from aerial parts of Scutellaria baicalensis, MeOH extracts from aerial parts of Scutellaria baicalensis were suspended with $H_2O$, and partitioned by $CHCl_3$. In order to investigate the efficacy of antioxidative activity the activity guided fraction and isolation of physiologically active substance were peformed. Its $H_2O,\;30\%,\;60\%$ MeOH and MeOH fractions were examined on antioxidative activity using DPPH method and TBARS assay; It was revealed that $30\%\;and\;60\%$ MeOH fractions have significant anti-oxidative activity. its fractions testing type I allergy, compound 48/80 induced systemic anaphylaxis was applied. As a result, compared with reference (cromolygate), these fraction significantly inhibited systemic anaphylaxis by $71\%\;and\;57\%$, respectively. From $30\%,\;60\%$ MeOH fraction, five compounds were isolated and elucidated apigenin 6-C-${\alpha}$-L-arabinopyranosyl-8-C-${\beta}$-D-glucopyranoside (isoschaftside, I), scutellarein 7-O-${\beta}$-D-glucuronopyranoside (scutellarin, II), apigenin 7-O- ${\beta}$-D-glucuronopyranoside (III), isoscutellarein 8-O-${\beta}$-D-glucuronopyranoside (IV), kaempferol 3-O-${\beta}$-D-glucopyranoside (V) through their physicochemical data and spectroscopic methods. We measured radical scavenging activity with DPPH method and anti-lipid peroxidative efficacy on human LDL with TBARS assay. [$I] showed antioxidant activities in order. Type I allergy compound 48/80 induced systemic anaphylaxis was applied. $[V inhibited systemic anaphylaxis in order.

• 생명과학회지
• /
• 제17권1호
• /
• pp.143-149
• /
• 2007

Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of retinoic acid receptor a (RARa). Rat cerebrocortical cells were grown in neurobasal medium. On DIV12 cells were treated with SB $(20{\mu}g/ml)$ and given a hypoxic shock $(2%\;O_2/5%\;CO_2,\;3hr)$ on DIV14. In situ hybridization using cRNA probe revealed that RARa mRNA punctae are distributed, in addition to nucleus, throughout neuronal dendrites, where SB upregulated its density by 69.8% (p=0.001) and 129.8% (p=0.001) in both normoxia and hypoxia, respectively. At the protein level, SB upregulated RARa in the neuronal soma by 78.8% (p=0.004) and 23.6% (p=0.001) in both normoxia and hypoxia, respectively. These results indicate that SB upregulates RARa in both normoxia and hypoxia, which might contribute to the neuroprotection.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 추계학술대회
• /
• pp.86-86
• /
• 2019

Scutellaria baicalensis Georgi (SB) has been widely studied to treat inflammatory diseases in east Asia. In the recent years, many studies have focused on modifying herbs to increase the pharmacological effects. Roasting alcohol absorbed SB is one of the traditional methods to increase the therapeutic effects. Currently there are no reports on the pharmacological effects of roasted SB. This study investigated the anti-inflammatory effects of roasted 30% ethyl alcohol absorbed SB extract (SR) on mice ear edema. After intra-gastric injection of dexamethasone (for positive control, 2 mg/kg) and SR (50, 100, 400 mg/kg), ear edema was provoked by croton oil (5% v/v in acetone, 10 ul/ear). Ear thickness was measured with a digital caliper to quantify the change in swelling. For histological study, we made paraffin sections and performed Phloxine-Tartrazine staining and Masson's trichrome staining to observe epidermis, dermis and subcutaneous region and collagen fiber of mice ear tissues. Ear thickness decreased dose-dependent manner in SR treated groups. Histological analysis compared with dexamethasone treated group, SR treated groups demonstrated a similar reduction in hypoplasia of epidermis and influx of inflammatory cells. Increase of subcutaneous layer and decrease of collagen fibers were significantly recovered in SR treated group (400 mg/kg) and dexamethasone treated group. In conclusion, treatment with SR ameliorates auricular inflammation induced with croton oil in mice. Experiments are now underway to understand the molecular mechanisms underlying anti-inflammatory activities of SR.

• 대한본초학회지
• /
• 제35권3호
• /
• pp.33-45
• /
• 2020

Objective : The present study aimed to evaluate the change of the content of 7 active components in decoctions produced by various proportional pairs of Coptis chinensis Franch and Scutellaria baicalensis Georgi in 'Mixed decoction (MD)' and 'Single decoction mixture (SDM)'. Methods : The samples of MDs were prepared by decocting C. chinensis : S. baicalensis with the ratios of 10 g:10 g, 10 g:20 g, and 20 g:10 g. Those of SDMs were prepared by blending each single decoction from C. chinensis and S. Baicalensis with the ratios of 1:1, 1:2, and 2:1. The samples were evaluated by high-performance liquid chromatography with statistical analyses. Results : The analytical methods, which were optimized and validated, were reliably applied to present research. The content of all components in both MDs and SDMs at C. chinensis : S. baicalensis = 1:1 ratio were reduced compared with single herb decoction. The components from each compositional herb in MDs were proportionally increased with the ratio of original herb increased, but inversely proportional to paired herb. The contents of components in MDs were significantly lower than those in SDMs at all ratios, except for high content of baicalin at C. chinensis : S. baicalensis = 2:1. Conclusion : It was concluded that MDs and SDMs as well as the proportions of herbs could affect the contents of the components from original herbal medicines. These results provide the information for the quality control of herbal medicine combined C. chinensis with S. baicalensis.

• 대한한방내과학회지
• /
• 제25권4호
• /
• pp.75-85
• /
• 2004

목적 : 黃芩(황금)과 黃芩(황금)의 주요 flavonoid 성분인 baicalein이 신장세뇨관 상피세포에서 산화제에 의한 apoptosis에 미치는 효과를 살펴보고자 한다. 방법 : 신장세뇨관 상피세포주인 opossum kidney (OK) 세포를 유기산화제인 t-butylhydroperoxide (tBHP)에 노출시켜 apoptosis를 일으킨 후 관련된 변화를 관찰하였다. 결과 : tBHP는 농도에 의존하여 apoptosis를 유발시켰는데, 이러한 효과는 黃芩(황금)과 baicalein에 의해 농도 의존적으로 방지 되었다. tBHP에 의한 OK 세포사는 항산화제인 Trolox와 N-acetylcysteine에 의해 방지 되었다. tBHP는 mitogen-activated protein kinase의 subfamily인 extracellular signal-regulated kinase (ERK)를 활성화시켰다. ERK 억제제인 PD98059와 U0126은 tBHP에 의한 세포 사망을 방지하였다. tBHP에 의한 ERK 활성화는 U0126에 의해 억제되었으나 黃芩(황금)과 baicalein에 의해서는 영향을 받지 않았다. 철착염제인 deferoxamine은 tBHP에 의한 세포 사망과 ERK 활성화를 방지하였다. tBHP에 의한 세포 사망은 casopase 억제제인 BOD-U-FMK와 zDEVD-FMK에 의해 방지되었다. 결론 : 黃芩(황금)은 산화제에 의한 세포 사망을 방지하는데, 이는 kinase 억제, 항산화제 역할 및 철착염제의 작용에 기인하지 않았다. 黃芩(황금)의 이러한 효과는 산화제에 의관 신부전 예방 및 치료제로 개발하는데 이용될 수 있는 가능성을 보였다.

• Journal of Microbiology and Biotechnology
• /
• 제6권3호
• /
• pp.209-212
• /
• 1996

A two-step cooling technique has been developed for cryopreservation of suspension cultured Scutellaria baicalensis cells. Efficient regrowth of cryopreserved cells was obtained in cryoprotected cells with a mixture of 1.5 M glycerol and 0.4 M sucrose in Schenk and Hildebrandt medium without pretreatment in high osmotic medium. Optimum freezing conditions were found to be a cooling rate of $0.5^{\circ}C$ min from $4^{\circ}C$ to $-40^{\circ}C$, and then retaining samples at $-40^{\circ}C$ for 30 min prior to plunging into liquid nitrogen. A regrowth rate of approximately 95$%$ was obtained after three month storage in liquid nitrogen. Callus cultures established from the cryopreserved cells were found to produce the same patterns of flavonoid accumulation and retain their baicalin producing activity.

• 한국약용작물학회지
• /
• 제4권2호
• /
• pp.178-181
• /
• 1996

황금재배실태(黃金栽培實態)와 문제점(問題點)을 파악(把握)하기 위하여 주산지(主産地)인 전남(全南) 여천군(麗川郡) 화양면(華楊面)에서 5년간(年間) 재배(栽培)한 결과(結果)를 대상(對象)으로 조사(調査)한 결과(結果)는 다음과 같다. 1. 맥후작(麥後作) 황금직파재배시(黃金直播栽培時) 제초제(除草劑) 전용약제(專用藥劑) 개발(開發)이 시급(時急)하다(현재(現在)로서는 인력(人力)으로 제초작업(除草作業)이 이루어 지고 있으므로 노동력(勞動力)이 부족(不足)하다) 2. 조숙다수성(早熟多收性) 보리품종(品種) 개발(開發)이 시급(時急)하다. 3. 내병다수성(耐病多收性) 양질(良質)의 황금품종육성(黃金品種育成)이 시급(時急)하다. 4 병충해방제(病蟲害防除)는 농업(農業)의 사용(使用)이 아닌 영양요구시비(營養要求施肥)와 유기농법(有機農法)에 의한 내병충성(耐病蟲性) 재배법개선연구(栽培法改善硏究)가 시급(時急)하다.

• 생약학회지
• /
• 제35권1호통권136호
• /
• pp.22-27
• /
• 2004

Scutellaria baicalensis Georgi is one of most important medicinal herbs in traditional chinese medicine. The object of this study was to determine the effects of the water extracts of Scutellaria baicalensis (SB) on the anxiolytic-like activities in the elevated plus-maze (EPM) test. The watεr extracts of SB (100, 200, or 400 mg/kg) were orally administered to male SD rats for 3 days, and behavioral tests for the anxiolytic activity were performed. SB (100, 200, or 400 mg/kg) significantly increased in time-spent and arm entries into the open arms of the EPM compared with the control group. Futhermore, those anxiolytic-like activities of SB were antagonized by flumazenil (a $GABA_A$ antagonist, 3 mg/kg), but not by pindolol (a $5-HT_{1A}$ antagonist, 10 mg/kg). SB did not cause myorelaxant effects in the horizontal wire test at any dosage regimen. Therefore, these findings suggest that SB promote the anxiolytic-like activity mediated by GABAergic nervous system in rats.

• 한국식품영양과학회지
• /
• 제42권10호
• /
• pp.1712-1716
• /
• 2013

통풍 증상을 개선하고 예방 및 치료에 효과를 확인하여 이를 일상생활에 지장을 주지 않고 장기간 쉽게 복용하기에 적합한 식품 소재를 발굴하기 위하여 한의학 및 식품 관련 서적에서 통풍 개선 및 치료 효과가 있어 널리 사용되고 있는 29가지 식용 가능 한약재를 선별하였다. 각 한약재의 열수추출액의 총 페놀 함량, DPPH 라디칼 소거능, ABTS 라디칼 소거능, xanthine oxidase 저해능을 측정한 결과, 황금추출액이 가장 높은 활성을 나타내었다. 이상의 결과는 황금추출액을 주 소재로 하여 항산화능과 통증 개선에 유용한 식품 개발에 활용될 수 있을 것으로 기대된다.