• 제목/요약/키워드: Screening system

검색결과 1,390건 처리시간 0.036초

Screening of saline tolerant plants and development of biological monitoring technique for saline stress . 1. Survey of vegetation in saline region and determination of saline tolerance of the plant species of the region. (내염성 식물의 탐색 및 생물학적 염해 모니터링 기술의 개발 1. 염해지 식생분석 및 식물종의 내염성 평가)

  • Kang, Byeung-Hoa;Shim, Sang-In
    • Korean Journal of Environmental Agriculture
    • /
    • 제17권1호
    • /
    • pp.26-33
    • /
    • 1998
  • This experiment was conducted to classify the plant species occurring in the saline reclaimed land by saline tolerance. The vegetation of reclaimed land was composed of various plant species from halophyte to glycophyte showing different saline tolerances. In the investigated saline region, reclaimed land of Youngjong island, Inchun city, 175 species belonging to 32 families were found. Our survey was carried out in two region, having different salinity due to different desalinization. The electricalconductivity (EC) of more saline region showed 48.0mS/cm nd the other region showed 13.0mS/cm. It is assumed that intensity of precipitation and runoff of rainfall may cause salinity gradient in the investigated region. The plant species occurred in the experimental region were classified as 72 species of annual, 42 species of biennial, and 61 species of perennial according to life cycle. For knowing relationship between vegetation of saline region and saline tolerance of occurring species, we tested the saline susceptibility of plant species collected at the saline regions. Testing plants were cultured by nutrient solution containing 200 mM NaCl, the critical concentration of survival in glycophytes. The saline tolerance was graded by the growing capacity in the sand-culture system. The more saline-tolerant species screened by sand culture were Atriplex gmelini, Suaeda asparagoides, Aster tripolium, Suaeda maritima, Salicornia herbacea, and Suaeda japonica. The most saline tolerant family was Chenopodiaceae. Poaceae, Cyperaceae, and Brassicaceae showed relatively high tolerance to saline stress. In the course of growth under the high saline condition, the most noticeable change was the darkening of leaves by increasing of chlorophyll content. The chlorophyll contents were increased with saline stress in most species.

  • PDF

Development of Prediction Model by NIRS for Anthocyanin Contents in Black Colored Soybean (근적외분광분석기를 이용한 검정콩 안토시아닌의 함량 분석)

  • Kim, Yong-Ho;Ahn, Hyung-Kyun;Lee, Eun-Seop;Kim, Hee-Dong
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • 제53권1호
    • /
    • pp.15-20
    • /
    • 2008
  • Near infrared reflectance spectroscopy (NIRS) is a rapid and accurate analytical method for determining the composition of agricultural products and feeds. This study was conducted to measure anthocyanin contents in black colored soybean by using NIRS system. Total 300 seed coat of black colored soybean samples previously analyzed by HPLC were scanned by NIRS and over 250 samples were selected for calibration and validation equation. A calibration equation calculated by MPLS(modified partial least squares) regression technique was developed in which the coefficient of determination for anthocyanin pigment C3G, D3G and Pt3G content was 0.952, 0.936, and 0.833, respectively. Each calibration equation was applied to validation set that was performed with the remaining samples not included in the calibration set, which showed high positive correlation both in C3G and D3G content file. In case Pt3G, the prediction model was needed more accuracy because of low $R^2$ value in validation set. This results demonstrate that the developed NIRS equation can be practically used as a rapid screening method for quantification of C3G and D3G contents in black colored soybean.

Effects of Organotin Compounds on Follicular Steroidogenesis in Frogs

  • Kwon, Hyuk-Bang;Kim, Seung-Chang;Kim, An-Na;Lee, Sung-Ho;Ahn, Ryun-Sup
    • Development and Reproduction
    • /
    • 제13권3호
    • /
    • pp.163-172
    • /
    • 2009
  • Some organotin compounds such as butyltins and phenyltins are known to induce impo-sex in various marine animals and are considered to be endocrine disruptors. In this study, the effect of organotins on follicular steroidogenesis in amphibians was examined using ovarian follicles of Rana dybowskii and Rana catesbeiana. Isolated follicles were cultured for 6 or 18 h in the presence and absence of frog pituitary homogenate (FPH) or various steroid precursors, and the levels of product steroids in the culture media oassay. Among the butyltin compounds, tributyltin (TBT) strongly and dose-dependently inhibited the FPH-induced synthesis of pregnenolone ($P_5$) and progesterone ($P_4$) by the follicles. TBT also strongly suppressed the conversion of cholesterol to $P_5$ and partially suppressed the conversion of $P_5$ to $P_4$. A high concentration of dibutyltin (DBT) also inhibited steroidogenesis by the follicles while monobutyltin and tetrabutyltin had negligible effects. The toxic effect of TBT or DBT was irreversible and a short time of exposure (30 min) was enough to suppress steroidogenesis. All the phenyltin compounds significantly inhibited FPH-induced $P_5$ synthesis by the follicles. The effective dose of 50% inhibition by diphenyltin was $0.04\;{\mu}M$ and those of monophenyltin and triphenyltin were $0.24\;{\mu}M$ and $0.3\;{\mu}M$, respectively. However, none of the phenyltin compounds significantly suppressed the conversion of $P_4$ to $17{\alpha}$-hydroxyprogesterone ($17{\alpha}$-OHP) (by $17{\alpha}$-hydroxylase), $17{\alpha}$-OHP to androstenedione (AD) (by $C_{17-20}$ lyase), or AD to testosterone by the follicles. Taken together, the data show that among the steroidogenic enzymes, P450scc in the follicles is the most sensitive to organotin compounds and that an amphibian follicle culture system can be a useful screening model for endocrine disruptors.

  • PDF

Rapid Gas Chromatographic Screening of Alcoholic Beverages for Volatile and Nonvolatile Organic Acids (기체크로마토그래피법에 의한 알코올 음료내 휘발성 및 비휘발성 유기산의 신속한 스크리닝)

  • Kim, Jung-Han;Kim, Kyoung-Rae;Chai, Jeong-Young;Park, Hyung-Kook;Choi, Kyoung-Sook
    • Korean Journal of Food Science and Technology
    • /
    • 제26권2호
    • /
    • pp.162-166
    • /
    • 1994
  • A rapid gas chromatographic (GC) profiling method for the simultaneous analysis of volatile and nonvolatile organic acids was applied to alcoholic beverages (white wine, red wine, brandy, and beer). It involves the solid-phase extraction of organic acids using Chromosorb P as the sorbent and diethyl ether as the eluent with subsequent triethylamine treatment. The resulting triethylammonium salts of acids were directly converted to volatile tert.-butyldimethylsilyl derivatives, which were analyzed by dual-capillary column GC and GC-mass spectrometry. From the alcoholic beverages studied, more than 29 organic acids were detected. When the simplified retention infer (RI) spectra of organic acids, and the direct comparisor method between alcoholic beverages and a test sample were attempted to identify a test sample, it was quickly recognized to be a red wine with the 998 ppt match quality value.

  • PDF

A Study on Obesity and Behavior Problems in Elementary Children Living in Poverty (학령기 빈곤가정 아동의 비만도와 문제행동 양상)

  • Cho, Kyung-Mi;Kim, Eun-Joo
    • Child Health Nursing Research
    • /
    • 제19권2호
    • /
    • pp.140-148
    • /
    • 2013
  • Purpose: The aim of this study was to explore the relationship of being from low-income families on children's behavior problems and obesity. Methods: The research design was a descriptive survey study, and the participants were 197 children from low-income families who took part in activities at a local children's center. They were selected through convenience sampling. The participants completed a questionnaire, BASC-2 (Behavior Assessment System for Children). Results: The results are as follows: 22.3% had obesity, a rate higher than that of children in general. Further, 37.3% had attention deficit problems, 33.8% had problems adapting to school and 49% had other adaption problem. There was a gender-specific difference with respect to all of the behavior problems. Conclusion: The results of this study indicate a lack of an identified child policy for behavior problems and health, and suggest a need for various programs and policies which could lead to development of behavior screening programs for children from low-income families.

Cell Patterning on Various Substrates Using Polyelectrolyte Multilayer and Microstructure of Poly(Ethylene Glycol) (다양한 기판 위에서 고분자 전해질 다층 막과 폴리에틸렌글리콜 미세 구조물을 이용한 세포 패터닝 방법)

  • Shim, Hyun-Woo;Lee, Ji-Hye;Choi, Ho-Suk;Lee, Chang-Soo
    • Korean Chemical Engineering Research
    • /
    • 제46권6호
    • /
    • pp.1100-1106
    • /
    • 2008
  • In this study, we presented rapid and simple fabrication method of functionalized surface on various substrates as a universal platform for the selective immobilization of cells. The functionalized surface was achieved by using deposition of polyelectrolyte such as poly(allyamine hydrochloride) (PAH), poly(diallyldimethyl ammonium chloride) (PDAC), poly(4-ammonium styrene sulfonic acid) (PSS), poly(acrylic acid) (PAA) and fabrication of poly(ethylene glycol) (PEG) microstructure through micro-molding in capillaries (MIMIC) technique on each glass, poly(methyl methacrylate) (PMMA), polystyrene (PS) and poly(dimethyl siloxane) (PDMS) substrate. The polyelectrolyte multilayer provides adhesion force via strong electrostatic attraction between cell and surface. On the other hand, PEG microstructures also lead to prevent non-specific binding of cells because of physical and biological barrier. The characteristic of each modified surface was examined by using static contact angle measurement. The modified surface onto several substrates provides appropriate environment for cellular adhesion, which is essential technology for cell patterning with high yield and viability in the micropatterning technology. The proposed method is reproducible, convenient and rapid. In addition, the fabrication process is environmentally friendly process due to the no use of harsh solvent. It can be applied to the fabrication of biological sensor, biomolecules patterning, microelectronics devices, screening system, and study of cell-surface interaction.

Review of Soil Vulnerability Assessment Tools in Korea and other developed countries (국내외 토양 취약성 평가 연구 동향)

  • Ki, Seo Jin;Kim, Kyoung-Ho;Lee, Hyeon Gyu;Shin, Kyung Hee
    • Journal of Korean Society of Environmental Engineers
    • /
    • 제39권12호
    • /
    • pp.741-749
    • /
    • 2017
  • This study aims to provide the technical considerations and implications for the development of soil vulnerability assesment tool based on the review of existing tools and case studies applied both domestically and internationally. For this study, we specifically investigated the basic theories and major features implemented in the screening models abroad. In contrast, one case study of prioritizing the vulnerable districts was presented to identify the research trends in Korea. Our literature review suggested that the characteristic of target areas and contaminants needed to be properly incorporated into soil vulnerability assessment because the current tools in Korea neglected these properties which prevented this tool from being used as a correct measure of soil management and prevention. We also reached the conclusion that in terms of technical aspect, the soil vulnerability assessment tool should be developed based on the physical theory and environmental data that were varied over space and time so that the end-users were able to readily and effectively screen soil vulnerability over large areas. In parallel with technical improvement, great effort needed to be devoted to develop an integrated environmental information system that increased the availability of data and shared various types of environmental data through enhanced multi-agency collaboration.

A Study on the Cooperation & Development Schemes of South-North Korea Logistics in the Age of Changes (변화시대 남북물류의 협력과 발전방안에 관한 연구)

  • Kim, Hong-Seop
    • Journal of Korea Port Economic Association
    • /
    • 제28권1호
    • /
    • pp.229-261
    • /
    • 2012
  • Concerning the political situations of Korea, after the coming of MB Administration the blockades between South and North has been deepened. Because of stopping of the tourism of Diamond Mountain, the Cheonan ship accidents and the ** bombardment of Yeonpyung island, the situation of confrontation of South and North is enlarged. After the death of Kim Jong-il, on 17 Dec. 2011, the new situations are opened in North Korea(NK). On the basis of economic cooperation between South and North, various logistical cooperation of South and North has been progressed. On November of 2002, the 1st Shipping Cooperation Meeting of South and North, which may be a basis of Shipping Agreement has been discussed. And the railway between South and North has been connected. This study, screening the change of South and North conditions, analyzed the tasks and current conditions of South and North logistics based on economic cooperation, with the previous researches and data of South and North Korea. And it grouped and suggested future development directions and practical agenda, after considering the recent changes. Furthermore it proposed the medium-long term logistical system of Korea peninsula and future strategies and vision. To solve the confrontation situations the role of South Korea is more important.

Screening of Natural Herb Resources for Anti-oxidative Effects in Korea (국내 자생 자원식물들의 항산화 활성 검색)

  • Yang, Yoon-Jung;Kim, Hyun-Jun;Kang, Shin-Ho;Kang, Se-Chan
    • Korean Journal of Plant Resources
    • /
    • 제24권1호
    • /
    • pp.1-9
    • /
    • 2011
  • In the present study, the anti-oxidative properties of 80% ethanol extracts from 140 kinds of natural plants were investigated. Persicaria perfoliata had the highest anti-oxidative relative potency(VitaminE, $ORAC_{PE}$=1.0), followed by Sorbaria sorbifolia var. stellipila, Rosa multiflora, Lysimachia vulgaris var. davurica, Quercus aliena, Quercus mongolica and Lespedeza bicolor ($ORAC_{PE}$ ${\geq}$ 1.50). Among them, the anti-oxidative activity values of the n-hexane, $CH_2Cl_2$, EtOAc, n-BuOH, and water fractions of the 80% EtOH extracts of P. perfoliata and S. sorbifolia in the ORAC assay system were $1.13{\pm}0.002$, $1.10{\pm}0.004$, $3.67{\pm}0.232$, $1.62{\pm}0.049$ and $1.05{\pm}0.003$; $1.02{\pm}0.007$, $1.00{\pm}0.016$, $2.94{\pm}0.130$, $1.80{\pm}0.019$ and $1.04{\pm}0.012$, respectively, with marked anti-oxidative activity in the ethyl acetate fraction from selected the 2 plant extracts.

Determination of Baicalin and Baicalein Contents in Scutellaria baicalensis by NIRS (근적외선분광분석기를 이용한 황금(Scutellaria baicalensis)의 baicalin 및 baicalein 함량 분석)

  • Kim, Hyo-Jae;Kim, Se-Young;Lee, Young-Sang;Kim, Yong-Ho
    • Korean Journal of Plant Resources
    • /
    • 제27권4호
    • /
    • pp.286-292
    • /
    • 2014
  • Near infrared reflectance spectroscopy (NIRS) is a rapid and accurate analytical method for determining the composition of agricultural products and feeds. This study was conducted to measure baicalin, baicalein, and wogonin contents in Scutellaria baicalensis by using NIRS system. Total 63 samples previously were analyzed by HPLC, which showed baicalin, baicalein, and wogonin contents ranging 4.56 to 13.59%, 0.28 to 5.54%, and 0.50 to 1.63% with an average of 9.66%, 2.09% and 0.52%, respectively. Each sample was scanned by NIRS and calculated for calibration and validation equation. A calibration equation calculated by modified partial least squares(MPLS) regression technique was developed in which the coefficient of determination for baicalin, baicalein, and wogonin content was 0.958, 0.944, and 0.709, respectively. Each calibration equation was applied to validation set that was performed with the remaining samples not included in the calibration set, which showed high positive correlation both in baicalin and baicalein content file. In case of wogonin, the prediction model was needed more accuracy because of low $R^2$ value in validation set. These results demonstrate that the developed NIRS equation can be practically used as a rapid screening method for quantification of baicalin and baicalein contents in Scutellaria baicalensis.