• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.30 no.11 s.254
• /
• pp.1472-1478
• /
• 2006

Double-deck train has been attracted growing attention as next generation transportation around metropolis because of high passenger carrying capacity. To develop high-speed double-deck train with low operational costs, the carbody must be designed as light as possible. In addition, the carbody must be strong enough to ensure the safety of passengers. To meet these design requirements, we perform systematically weight minimization that determines thickness of aluminum extruded panels of the carbody. First, to reduce the design variables, we carry out the screening process that select sensitive or/and important design variables through design exploration. Then, weight minimization is accomplished under multi-loading condition such as vertical, compressive and torsional loads, while satisfying strength constraints of the design regulations. Finally, the result of design optimization is discussed by comparison with its initial design.

• KSBB Journal
• /
• v.23 no.5
• /
• pp.398-402
• /
• 2008

Cyanobacteria havebeen identified as one of the most promising group producing novel biochemically active natural products. Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Though many useful natural products have been identified in cyanobacterial biomass, cyanobacteria produce also extracellular proteins related with NRPS/PKS. Detection of unknown secondary metabolites in medium was carried in the present study by a screening of 98 cyanobacterial strains. A degenerated PCR technique as molecular approaches was used for general screening of NRPS/PKS gene in cyanobacteria. A putative PKS gene was detected by DKF/DKR primer in 38 strains (38.8%) and PCR amplicons resulted from a presence of NRPS gene were showed by MTF2/MTR2 primer in 30 strains (30.6%) and by A3/A7 primer in 26 strains (26.5%). HPLC analysis for a detection of natural products was performed in extracts from medium in which cyanobacteria containing putative PKS or NRPS were cultivated. CBT57, CBT62, CBT590 and CBT632 strains were screened for a production of extracellular natural products. 5 pure substances were detected from medium of these cyanobacteria.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.14 no.12
• /
• pp.1279-1286
• /
• 2004

This paper continues companion paper, part I : measurement and analysis. As shown in companion Paper, information and energy in monaural signal is quite different from that of binaural signal. In this paper, difference between monaural and binaural signal of transportation noise are investigated in subjective response test. We executed hearing screening test before giving a subject response test and excluded subjects who had physical hearing loss. An annoyance response test was conducted using headphone to avoid cross-talk effect in binaural testing. Percentage of highly annoyed under binaural signal reproduction is higher than percentage of highly annoyed under monaural signal reproduction. Result implies binaural reproduction technique is proper for a study of human response to short-term noise exposure in a headphone simulated-environment.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.6
• /
• pp.993-1001
• /
• 2007

The technique of serological analysis of antigens by recombinant cDNA expression library (SEREX) uses autologous patient sera as a screening probe to isolate tumor-associated antigens for various tumor types. Isolation of tumor-associated antigens that are specifically reactive with patient sera, but not with normal sera, is important to avoid false-positive and autoimmunogenic antigens for the cancer immunotherapy. Here, we describe a selection methodology to isolate patient sera-specific antigens from a yeast surface-expressed cDNA library constructed from 15 patient lung tissues with non-small cell lung cancer (NSCLC). Several rounds of positive selection using patient sera alone as a screening probe isolated clones exhibiting comparable reactivity with both patient and normal sera. However, the combination of negative selection with allogeneic normal sera to remove antigens reactive with normal sera and subsequent positive selection with patient sera efficiently enriched patient sera-specific antigens. Using the selection methodology described here, we isolated 3 known and 5 unknown proteins, which have not been isolated previously, but and potentially associated with NSCLC.

• Journal of the Korean Magnetic Resonance Society
• /
• v.9 no.1
• /
• pp.29-37
• /
• 2005

Intra-cellular drug uptake in Xenopus laevis oocyte has been elucidated using localized MR spectroscopy (MRS) and PFG NMR techniques at a 600 $MH_z$(Bruker, 14.1 T) NMR spectrometer. The localized MRS has been done with a homemade probe, and shows the intra-cellular uptake of nicotinamide. The self-diffusion of the molecule in Xenopus oocyte was obtained by PFG NMR technique. The measured data are well fitted with a linear combination of two exponential functions, which shows that there are two types of drug molecules, intra-and extra-cellular molecules. Diffusion coefficients of intra- and extra-cellular drug molecules are 3.7 $\times$ $10^{-11}$ $\m^{2}/s$and 6.4 $\times$ $10^{-10}$ $\m^{2}/s$, respectively. In the weighting factors there is shown that about 5% of drug molecule is inside the cells. These techniques can be used for drug screening in molecule-, cell-, and tissue-based preclinical test.

• Journal of the Korean Society of Tobacco Science
• /
• v.36 no.1
• /
• pp.26-33
• /
• 2014

Simultaneous determination of crop protection agents(CPAs) in food are done with multi-residue methods, which are composed of sample clean-up, concentration, chromatographic separation and detection. Stir Bar Sorptive Extraction(SBSE) technique is used for sample preparation of various analytes in several fields. The aim of this study was to develop a sensitive and fast method based on SBSE followed by thermal desorption - gas chromatography - mass spectrometry(TD - GC/MS) to determine CPAs in tobacco sample. For the analysis of tobacco sample prior to the SBSE method, solvent extraction or ultrasound-assisted solvent extraction was performed. methanol was used as the extraction solvent. The extract was then diluted with water. Finally, the sample was subjected to SBSE. A method for fast screening of crop protection agents in tobacco using SBSE-TD - GC/MS has been developed. About 17 CPAs including organochlorine, organophosphorous and others were identified and quantified. This method showed good linearity and high sensitivity for most of the target CPAs. The method was applied to the determination of CPAs at ng/mL levels in tobacco sample. This method is simple, rapid and may be applied in detection of other components.

• Molecular & Cellular Toxicology
• /
• v.2 no.4
• /
• pp.251-256
• /
• 2006

The exploration of genes which expressions are changed by exposure to ecotoxicants or pollutants can provide the important information about the reaction mechanisms in the body as well as adaptation to exterior stimulus or environmental changes. Also they can be developed as biomarkers for the detection of environmental pollution. Differential display polymerase chain reaction (DD-PCR) technique has been usefully used to hunt the clones which expressions are up-regulated or down-regulated by exterior changes and this study aimed to search for those clones in diesel oil-exposed rockfish (Sebastes schlegeli) using DD-PCR. The RNA isolated from liver of 20 ppb diesel oil-exposed rockfish was used for screening of the differentially displayed genes and total 44 differentially expressed genes (DEG) are detected then their nucleotide sequences were analyzed. The present data provided the general information about the effect of diesel oil contamination on marine organism and further more the primary step in development of new biomarkers for marine environmental pollution or ecotoxicological stresses.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.24
• /
• pp.10927-10932
• /
• 2015

Background: Older age is associated with an increase in the incidence of cancer cases. Diagnosis and treatment of cancer in older adults can be stressful because of health decline related to age, comorbidities and inadequate treatment for pain and other symptoms of the disease. This survey is one of the unique studies in Jordan and in the Arab world that aimed at exploring the predictors of older adult individuals awareness towards cancer. Materials and Methods: A stratified random sampling technique was followed to reach a representative sample of 753 participants. The questionnaire of the study consisted of parts regarding demographic variables, awareness about screening tests, and signs and symptoms of cancer. In addition, specific questions related to factors linked to cancer were included. Cross-sectional design was adopted with face-to-face interviews at the interviewees' households. Results: The mean age of the participants was about 63 years; around 44% of them are above the age of 65 years. 'Being convinced that having no health problem makes the individual feel safe and not at risk of getting cancer' was the major reason for not doing a routine health check-up. Conclusions: The low levels of awareness about cancer, as well as the low use of the screening methods were crucial outcomes of this study. Thus, raising the awareness among health care providers and policy makers in the country about older adults' perception of cancer is a high priority.

• Journal of Oral Medicine and Pain
• /
• v.24 no.2
• /
• pp.181-187
• /
• 1999

The most important progress in diagnostic sciences is the increased sensitivity and specificity in diagnostic procedures due to the development of newer micromethodologies and increasing availability of immunological and molecular biological reagents. The outcome of researches in this field has already provided DNA probes and antibodies which can be used for diagnosing various kinds of diseases including inherited ones. This development can be also applied to diagnose diseases in oral and maxillofacial regions. Technological advances have yielded highly sensitive test methodologies so that low analyte concentration and small sample volume are no longer limiting factors. Therefore, saliva can be useful test fluid for an array of analytes. Salivary constituents of diagnostic significance include steroid hormones, antibodies, drugs, and tumor markers. Of the proteins present in saliva, viral-specific immunoglobulins are of the greatest diagnostic interest. The development of conjugates and antigens by recombinant DNA technique and peptide synthesis is necessary for clinical application. Several kits developed for the purpose of blood testing should be modified to permit their application to saliva. The final practical outcome of researches in diagnostic sciences will be various diagnostic agents which can be used for detection of bacteria and viruses, screening and diagnosis of diseases, genetic screening for forensic individual identification. For these purposes, collaboration researches and development between institutions and companies are essential.

• Journal of Life Science
• /
• v.21 no.6
• /
• pp.865-874
• /
• 2011

The genus Bryophyllum is best known for many of its species having the ability to produce plantlets on their leaves. This phenomenon is also known as vegetative reproduction. Differential expressed gene (DEG) detecting technique was applied in order to survey the genes involved in the process of asexual reproduction for plantlet formation. Based on homology search using the NCBI database after screening of genes, 38 genes were identified from a total of 69 DEGs. Most of these DEGs were related to cell division, to intercellular signal transduction, and to hormone (cytokinin and ethylene) signaling.