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Extracellular Products from Cyanobacteria  

Kwon, Jong-Hee (Fachgebiet Technische Bioverfahrenstechnik Institut fur Biotechnologie Technische Universitat Berlin)
Kim, Gi-Eun (Department of Biotechnology, Seokyeong University)
Publication Information
KSBB Journal / v.23, no.5, 2008 , pp. 398-402 More about this Journal
Abstract
Cyanobacteria havebeen identified as one of the most promising group producing novel biochemically active natural products. Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Though many useful natural products have been identified in cyanobacterial biomass, cyanobacteria produce also extracellular proteins related with NRPS/PKS. Detection of unknown secondary metabolites in medium was carried in the present study by a screening of 98 cyanobacterial strains. A degenerated PCR technique as molecular approaches was used for general screening of NRPS/PKS gene in cyanobacteria. A putative PKS gene was detected by DKF/DKR primer in 38 strains (38.8%) and PCR amplicons resulted from a presence of NRPS gene were showed by MTF2/MTR2 primer in 30 strains (30.6%) and by A3/A7 primer in 26 strains (26.5%). HPLC analysis for a detection of natural products was performed in extracts from medium in which cyanobacteria containing putative PKS or NRPS were cultivated. CBT57, CBT62, CBT590 and CBT632 strains were screened for a production of extracellular natural products. 5 pure substances were detected from medium of these cyanobacteria.
Keywords
cyanobacteria; extracellular product; secondary metabolite; non ribosomal peptide; etc;
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