• 한국균학회지
• /
• 제50권2호
• /
• pp.143-147
• /
• 2022

Sclerotinia rot symptoms were observed in stringy stonecrop (Sedum sarmentosum) plants growing in vinyl greenhouses in Yeoju and Icheon, Gyeonggi Province, Korea, during disease surveys in spring 2019 and 2020. The initial symptoms were soft rot on stems and leaves at or above the soil line. Furthermore, the symptoms progressed upwards, and the infected plant parts exhibited white to grayish-yellow discoloration. The infestation of diseased plants in the vinyl greenhouses was 1-5% at the two locations examined. Eight isolates of Sclerotinia sp. were obtained from lesions of the diseased plants. The isolates were identified as Sclerotinia sclerotiorum based on their morphological and molecular characteristics. In addition, artificial inoculation tested three isolates of S. sclerotiorum for pathogenicity on stringy stonecrop plants. All the tested isolates caused Sclerotinia rot symptoms in the inoculated plants. The symptoms were similar to those observed in plants from the vinyl greenhouses investigated. This study is the first report of S. sclerotiorum causing Sclerotinia rot in stringy stonecrop.

• Mycobiology
• /
• 제31권2호
• /
• pp.113-118
• /
• 2003

Solanaceous crops grown in greenhouses and fields in Korea were surveyed from 1994 to 2000. Sclerotinia rot most severely occurred up to 60% in potato. Incidence of the disease was as high as 20% at its maximum in tomato and 5% in eggplant, but as low as less than 1% in red pepper. Symptoms of Sclerotinia rot commonly developed on stems of the solanaceous crops but rarely on fruits of eggplant and tomato. A total of 169 isolates of Sclerotinia species was obtained from the diseased solanaceous crops. Out of the isolates, 165 isolates were identified as S. sclerotiorum, and the others as S. minor based on their morphological and cultural characteristics. S. sclerotiorum was isolated from all the solanaceous crops, while S. minor was only isolated from tomato. Eight isolates of S. sclerotiorum and two isolates of S. minor were tested for their pathogenicity to the solanaceous crops by artificial inoculation. All the isolates of the two Sclerotinia spp. induced rot symptoms on stems of the solanaceous crops tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility of the solanaceous crops to the isolates of S. sclerotiorum. However, in case of S. minor, the potato cultivar Sumi was relatively less susceptible to the pathogen.

• The Plant Pathology Journal
• /
• 제19권1호
• /
• pp.69-74
• /
• 2003

Cruciferous crops grown in greenhouses and fields in Korea were surveyed from 1995 to 2000. Sclerotinia rot most severely occurred up to 30％ in cabbage. Incidence of the disease was as high as 20% at its maximum in Chinese cabbage and rape and 10％ in radish, but as low as less than 1 or 2％ in broccoli and kale. Symptoms of Sclerotinia rot commonly developed on loaves and stems of the crucifers, but rarely on rachises of broccoli. A total of 112 isolates of sclerotinia species was obtained from the diseased crucifers. Out of the isolates, 103 isolates were identified as S. sclerotiorum, and the rest as 5. minor based on their morphological and cultural characteristics. S. sclerotiorum was isolated from all the crucifers, while S. minor was isolated from Chinese cabbage, broccoli, and kale. Six isolates of S. sclerotiorum and three isolates of S. minor were tested for their pathogenicity to the crucifers by artificial inoculation. All the isolates of the two Sclerotinia spp. induced rot symptoms on the plants of the crucifers tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility of the crucifers to the isolates of S. sclerotiorum. However, in case of S. minor, radish was relatively less susceptible to the pathogen.

• 한국균학회지
• /
• 제39권2호
• /
• pp.131-135
• /
• 2011

2종의 상추균핵병균(S. sclerotiorum, S. minor)에 기생성인 선발진균(CM2)의 형태적 및 분자적 특성을 조사한 결과, Paraconiothyrium minitans로 동정되었다. P. minitans CM2의 균사 배양조건은 pH 6.5로 조절한 PDA배지를 사용하고, $22^{\circ}C$에서 1일 12시간 주기의 근자외선광을 처리하여 배양하는 것이 가장 좋은 것으로 나타났다. P. minitans CM2의 분생포자현탁액을 상추균핵병균의 균핵에 처리하여 발아에 미치는 영향을 조사한 결과, 균핵의 직접발아와 자낭반 형성이 전혀 이루어지지 않았다.

• Mycobiology
• /
• 제34권3호
• /
• pp.154-157
• /
• 2006

A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were $25^{\circ}C$ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum. The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries.

• 생명과학회지
• /
• 제14권5호
• /
• pp.761-769
• /
• 2004

부산시 강서구 강동동 지역의 들깨 하우스내에서 들깨의 잎에 갈색내지 흑갈색의 병반이 형성되고 습도가 높을 시에는 잎에 무름증상과 줄기에 잘록증상이 나타나며, 심하면 주전체가 고사하여 흰색의 균사로 뒤덮히고 균핵이 형성되는 병해가 발생하여 1998년 5월에 자연발생된 균핵병 발병율을 조사한 결과 약 8.1～28.3%로서 평균 13.9%였다. 이러한 증상을 띄는 병반으로부터 분리된 16균주를 균사절편 접종법으로 병원성 검정한 결과, 접종 1일 후부터 발병되기 시작하여 자연발생된 병징과 동일한 증상을 나타내었으며 16균주 모두 80.0% 이상의 발병율을 나타내었다. 그 중 병원성이 가장 강한 52균주를 공시선발하고, 형태적 및 배양적 특성 등을 조사하여 동정한 결과, 들깨 균핵 병균 Sclerotinia sclerotiorum으로 동정되었다. 생물학적 방제를 위해 길항세균인 Nl 등 6균주를 공시하고 병원균 52균주와 균사생육 억제효과 및 생육상내 포트검정을 통해 방제효과를 검정한 결과, N4 균주에 의한 균사생육 억제효과와 방제효과가 가장 우수하였으며, 발병예방 및 치료효과 검정시 길항균을 병원균처리 1～3일 전에 처리할 경우 80.0% 이상의 방제가를 보여 치료 효과보다는 발병예방효과가 효과적인 것으로 확인되었다. 길항균 N4 균주를 Bergey's manual과 API system을 이 용하여 동정한 결과, Bacillus megaterium으로 동정 되었으며, 이를 수화제형인 미생물농약 N4제제로 제조하여 유기합성 농약인 베노밀수화제와 발병예방 및 치료효과를 비교하였다. 그 결과, 병원균처리 3일 전에 처리할 경우 N4 수화형 제제의 방제가가 98.0%로 베노밀수화제에 의한 78.0%보다 방제가가 높았다.

• The Plant Pathology Journal
• /
• 제15권4호
• /
• pp.236-241
• /
• 1999

Sclerotinia twig blight on trees and cottony rot on fruits of Satsuma mandarin were observed in Cheju, Korea in 1996 and 1997. The causal fungus causing citrus twig blight and cottony rot was identified as Sclerotinia sclerotiorum which had cup-shaped apothecia bearing hyaline and clavate asci and periphyses on sclerotium. Symptoms were produced on twigs, developing young leaves, fully developed new leaves, and fruits 2-9 days after wound inoculation, but only on twigs with young lateral buds and developing young leaves by unwound inoculation. The fungus grew well between 10 and $27^{\circ}$, but optimum temperature was $24^{\circ}$ on potato dextrose agar. Most varieties were highly susceptible to S. sclerotiorum, whereas some varieties such as Ichinan were relatively resistant among twelve Satsuma mandarin varieties in the field. This is the first record of Scledrotinia twig blight on trees and cottony rot on stored fruits of Satsuma mandarin in Korea.

• The Plant Pathology Journal
• /
• 제22권1호
• /
• pp.16-20
• /
• 2006

Four leguminous crops grown in greenhouses and fields in Korea were surveyed from 2000 through 2002. Sclerotinia rot most severely occurred up to $60\%$ in Phaseolus vulgaris grown in greenhouses but occurred as low as $0-0.5\%$ in that grown in fields. Incidence of the disease in Pisum sativum grown in greenhouses ranged $1-5\%$, and that in Vicia Java and Vigna sinensis grown in fields was $0.8\%$ and $2\%$, respectively. Symptoms of Sclerotinia rot commonly developed on stems and pods of the crops. A total of 59 isolates of Sclerotinia species were obtained from diseased stems and pods of the crops. All of the isolates were identified as Sclerotinia sclerotiorum based on their morphological characteristics. Eight isolates of the fungus were tested for their pathogenicity to four host crops by artificial inoculation. All of the isolates induced rot symptoms on stems of the host crops tested, which were similar to those observed in the fields. The pathogenicity tests revealed that there was no significant difference in the susceptibility to the isolates among the leguminous crops tested This is the first formal report that S. sclerotiorum causes the Sclerotinia' rot of the four leguminous crops in Korea.

• 식물병연구
• /
• 제8권2호
• /
• pp.113-116
• /
• 2002

A sclerotinia rot of Leonurus sibiricus caused by Sclerotinia sclerotio겨m occurred severely in farmer's fields at Seosang-myon, Hamyang-gun, Gyeongnam Province, Korea in 2001. The typical symptoms of the disease were that leaves, stems or collar of the infected plants at first became darker green and then watery soft rotted, and white fluffy mycelia formed on the lesion developed into black sclerotia later, Sclerotia on the plant and PDA medium were globose to cylindrical or irregular in shape, and 0.8~10.3$\times$0.8~6.4 m in size. Aphothecia with numerous asci were cup-shape, and 0.6~1.2 cm in diameter, Asci with 8 spores were cylindrical, and 87~246$\times$4.6~21.6 $\mu\textrm{m}$ in size. Ascospores of one cell were hyaline, ellipsoid to ovoid in shape, and 10.2~14.6$\times$4.7~7.2 $\mu\textrm{m}$ in size. The range of temperature for mycelial growth was from 5 to 3$0^{\circ}C$, and the optimum was $25^{\circ}C$. This is the first report on the sclerotinia rot of L. sibiricus caused by Sclerotinia sclerotioum in Korea.

• 식물병연구
• /
• 제9권1호
• /
• pp.47-51
• /
• 2003

남양주, 양주, 양평에서 파드득나물 균핵병의 발생생태를 조사한 결과, 균핵병은 늦가을부터 초봄까지 주로 동계기간에 발생했으며, 지역별로는 양평에서 발생이 심했다. 포장에서 분리한 파드득나물 균핵병균은 기보고된 Sclerotinia sclerotiorum의 균학적 특성과 일치하였고, 균사생장은 5~3$0^{\circ}C$에서 이루어졌으며, 15~3$0^{\circ}C$에서 활발하게 생장하였다 균핵형성은 20~$25^{\circ}C$에서 많이 형성되었으며, 낮은 온도에서 형성된 균핵의 크기가 큰 경향이었다. 균핵생존율은 균핵크기가 작을수록(~2 mm > 3~4 mm > 5 mm~) 높았으며, 치상온도에서의 정치시간이 길수록 높게 나타났다.