• Title/Summary/Keyword: Science planning

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Expression, Purification and Antiserum Production of the Avian Influenza H9N2 Virus HA and NA Proteins (Avian Influenza H9N2 Virus의 HA와 NA 단백질 발현, 정제 및 항혈청 생산)

  • Lee, Hyun-Ji;Song, Byung-Hak;Kim, Jeong-Min;Yun, Sang-Im;Kim, Jin-Kyoung;Kang, Young-Sik;Koo, Yong-Bum;Jeon, Ik-Soo;Byun, Sung-June;Lee, Youn-Jeong;Kwon, Jun-Hun;Park, Jong-Hyeon;Joo, Yi-Seok;Lee, Young-Min
    • Korean Journal of Microbiology
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    • v.44 no.3
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    • pp.178-185
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    • 2008
  • Avian influenza virus (AIV) is recognized as key to the emergence of pandemic influenza for humans; there are growing concerns that AIV H9N2 may become more efficient to transmit to humans in the near future, since the infection of poultry with AIV H9N2 has been common in recent years. In this study, we aimed to produce antisera recognizing the HA and NA proteins of AIV H9N2. Initially, coding sequences corresponding to the N-terminal regions of the HA and NA proteins of the Korean AIV H9N2 (A/Ck/Kr/MS96/96) isolated from a domestic chicken were amplified from the genomic RNA. Following cloning of the amplified cDNA fragments into pGEX4T-1 vector, two GST-fusion proteins (GST-HAln and GST-NAn) were expressed in E. coli BL21 and purified with glutathione sepharose columns; the recombinant GST-HAln and GST-NAn proteins were both used as immunogens in rabbits. The antigenicity of the rabbit antisera was analyzed by immunoblotting of the cell lysates prepared from AIV H9N2-infected MDCK cells. Overall, the recombinant HAln and NAn proteins fused to the C-terminus of GST and the rabbit antisera raised against the corresponding recombinant proteins would provide a valuable reagent for AIV diagnosis and basic research.

Characteristics of Bird Community in Sihwa South Grassland, Korea (시화호 남측 초지지역의 조류 군집 특성)

  • Park, Chi-Young;Kim, Ho-Joon;Paik, In-Hwan;Yu, Jae-Pyoung;Paik, Woon-Kee;Lee, Joon-Woo
    • Korean Journal of Environment and Ecology
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    • v.29 no.4
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    • pp.516-524
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    • 2015
  • This study was characteristics of bird community conducted to from 2009 to 2010 in Sihwa South Grassland, Gyeonggi-do, Korea. Total 49 species were recorded and the sum of maximum counts was 2,258 individuals. Species diversity was 2.21 and the species richness was 6.22. In South Reclaimed, we recorded maximum score of 38 species. and maximum score of 1,083 individuals in Dinosaur Egg Fossil. Comparison between areas were use average species diversity and species richness. Mostly Dinosaur Egg Fossil and South Reclaimed were higher than Sihwa Lake upstream. As a results of the ANOVA test, all areas were different. also, Sihwa Lake upstream only difference was that the bird community. In the season there was a significant difference between the Number of species and species richness. Number of species and species richness were significant differences depending on the season. but individuals and species diversity were no significant differences. In the year there was a no significant difference between the Number of species and individuals. but species diversity and species richness were significant differences. As a result, Between areas was different patterns of bird communities. but including water space Dinosaur Egg Fossil and South Reclaimed were showed a similar bird communities and bird diversity was higher. In the season, the resident was using of consistent patterns. but, There were differences depending on the migratory patterns of populations. In the year, every year was communities of a similar patterns. Sihwa South Grassland is of the high conservation value ecosystem as the only domestic. Propose an opinion for maintaining biodiversity when planning future habitat composition and environmental decrease.

Devonian Strata in Imjingang Belt of the Central Korean Peninsula: Imjin System (임진강대의 중부 고생대층: 임진계)

  • Choi, Yong-Mi;Choh, Suk-Joo;Lee, Jeong-Hyun;Lee, Dong-Chan;Lee, Jeong-Gu;Kwon, Yi-Kyun;Cao, Lin;Lee, Dong-Jin
    • The Journal of the Petrological Society of Korea
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    • v.24 no.2
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    • pp.107-124
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    • 2015
  • The 'Imjin System' (or Rimjin System) was established in 1962 as a new stratigraphic unit separated from the Upper Paleozoic Pyeongan System based on the discovery of brachiopods and echinoderms of possible Devonian age. Subsequent discoveries of the Middle Devonian charophytes confirmed the Devonian age of the system. The Imjin System is distributed in the Imjingang Belt between the Pyongnam Basin and the Gyeonggi Massif, spans from the eastern areas including Cholwon-gun of the Gangwon Province, Gumchon-gun, Phanmun-gun, and Tosan-gun of the Hwanghaebuk Province, to the western areas of Gangryong-gun and Ongjin-gun of the Hwanghaenam Province, and includes the Yeoncheon Group (metamorphic complex) to the south. Unlike the lower Paleozoic strata in the Pyongnam Basin which solely produce marine invertebrate fossils, the Imjin System yields diverse non-marine plant and algal fossils. Brachiopods of the system are similar to those from the Devonian of the South China Block and include taxa endemic to the platform, implying a close paleogeographic affinity to the South China Block. The Imjin System is generally considered as of Middle to Late Devonian in age, although there have been suggestions that the system is of the Middle Devonian to Carboniferous in age. North Korean workers postulated that the Imjin System was deposited in the current geographic position, where the "Imjin Sea" (an extension of the South China Platform) was located during the Devonian. The Imjin System displays strong local variations in stratigraphy and its thickness. It has recently been reported that the strata are repeated and overturned by thrust faults in many exposures. The Yeoncheon Group a southward extension of the Imjin System, also experienced intense tight folding and contractional deformation. Northward decrease in metamorphic grade within the system suggests that the northern part of the Gyeonggi Massif and the Imjingang Belt are probably an extension of the Dabie-Sulu Belt between the South China and Sino-Korean blocks, and the Imjin System is an remnant of accretion resulted from the collision between the two blocks. In order to understand tectonic evolution and Paleozoic paleogeography of eastern Asia, further studies on stratigraphic, sedimentologic and tectonic evolution of the Imjin System involving scientists from the two Koreas are urgently needed.

Analysis of Garden Preferences of Visitors to Korea Landscapes and Gardens Expo in 2017 - Focused on Location and Plant Characteristics - (2017년 대한민국 조경·정원박람회 방문객을 대상으로 한 정원선호도 분석 - 조성 장소 및 식물 특성을 대상으로 -)

  • Lee, Dong-Jin;Roh, Hee-Young;Kim, Min;Cho, Hyo-Jin;Lee, He-Min;Cho, Dong-Gil;Song, Yu-Jin;Cho, Hye-Ryeong;Chon, Jin-Hyung
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.6
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    • pp.126-136
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    • 2017
  • Gardeners have a desire to not only enjoy a garden but to create the garden themselves and play the role of garden designer. However, they express a great difficulty in gardening activities such as planting and managing plants, the main materials of the garden, due to lack of expertise. Therefore, in order to encourage hobby gardening activities, it is necessary to understand the preferences of the gardeners so that they can easily experience and create a garden, and to provide information on gardening know-how and preferred plants according to places given high preference. This study analyzed the preference for places and plants characteristics according to the purpose of creating a garden. To this end, a questionnaire survey was conducted with visitors to the Korea Landscape and Garden Expo, expected to have been attended primarily by gardening enthusiasts. As a result, gardeners were divided into three groups: appraisal - purposed type, multifunctional type, and participation - purposed type. The group of appraisal purposed type preferred porches and terraces, and private home front yards as well as favored indoor places rather than outdoor places compared with other types. In selecting plants, they prefer plants that can be easily managed in an indoor environment and appreciate natural colors through the colors of flowers and leaves. The multifunctional type group preferred a private home front yard as a garden creation space. Compared with the other types, it showed a high preference for 'unoccupied land in urban housing' and 'community garden' and was analyzed as a group having a high understanding of garden activities. In selecting plants, this group preferred fruit trees or productive plants such as homegrown crops. The participation purposed type group preferred porches and terraces as well as private home front yards as a gardening space. For plants, they preferred plants that are easy to manage. It is meaningful that this study provided preferences for places and plant characteristics according to the creative purpose of the gardener and provided basic data for selecting the place and the plant characteristics of the garden according to the gardener's type.

An historical analysis on the carbon lock-in of Korean electricity industry (한국 전력산업의 탄소고착에 대한 역사적 분석)

  • Chae, Yeoungjin;Roh, Keonki;Park, Jung-Gu
    • Journal of Energy Engineering
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    • v.23 no.2
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    • pp.125-148
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    • 2014
  • This paper performs a historical analysis on the various factors contributing to the current carbon lock-in of Korean electricity industry by using techo-institutional complex. The possibilities of the industry's carbon lock-out toward more sustainable development are also investigated. It turns out that market, firm, consumer, and government factors are all responsible for the development of the carbon lock-in of Korean power industry; the Korean government consistently favoring large power plants based on the economy of scale; below-cost electricity tariff; inflation policy to suppress increases in power price; rapid demand growth in summer and winter seasons; rigidities of electricity tariff; and expansion of gas-fired and imported coal-fired large power plants. On the other hand, except for nuclear power generation and smart grid, environment laws and new and renewable energy laws are the other remaining factors contributing to the carbon lock-out. Considering three key points that Korea is an export-oriented economy, the generation mix is the most critical factor to decide the amounts of carbon emission in the power industry, and the share of industry and commercial power consumption is over 85%, it is unlikely that Korea will achieve the carbon lock-out of power industry in the near future. Therefore, there are needs for more integrated approaches from market, firm, consumer, and government all together in order to achieve the carbon lock-out in the electricity industry. Firstly, from the market perspective, it is necessary to persue more active new and renewable energy penetration and to guarantee consumer choices by mitigating the incumbent's monopoly power as in the OECD countries. Secondly, from the firm perspective, the promotion of distributed energy system is urgent, which includes new and renewable resources and demand resources. Thirdly, from the consumer perspective, more green choices in the power tariff and customer awareness on the carbon lock-out are needed. Lastly, the government shall urgently improve power planning frameworks to include the various externalities that were not properly reflected in the past such as environmental and social conflict costs.

Dietary behavior and nutritional status among Chinese female college students residing in Korea (재한 중국 유학 여대생들의 식습관 변화에 따른 영양 섭취 실태)

  • Gaowei, Gaowei;Kim, Soyeon;Chang, Namsoo;Kim, Ki Nam
    • Journal of Nutrition and Health
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    • v.46 no.2
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    • pp.177-185
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    • 2013
  • The purpose of this study was to analyze the nutritional status of Chinese female college students in Korea, and to investigate changes in their dietary behavior after residing in Korea. The subjects included 114 Chinese female college students currently studying in Korea. General characteristics and dietary behaviors before and after residing in Korea were investigated. Daily nutrients and food intake were assessed using a one-day 24-hour recall. Consumption of most nutrients, namely calcium, iron, zinc, vitamin $B_2$, vitamin C, and folate was estimated to fall below the EAR for the Korean population. The proportions of subjects whose intake were estimated below the EAR for folate, calcium, vitamin $B_2$, iron, zinc, and vitamin C were 93.0%, 71.9%, 66.7%, 65.8%, 65.8%, and 63.2%, respectively. In the current study, the dietary behavior scores fell significantly after Chinese students immigrated to Korea (p < 0.001); increased frequency of meal skipping, less consumption of fruits and vegetables, irregular meal pattern, and imbalanced diet were some of the significant changes among Chinese students before and after residing in Korea. In addition, consumption of most nutrients, including dietary fiber, vitamin A, vitamin B group, vitamin C, and folate in the Worsened Group was significantly lower, compared with the No Change Group (p < 0.05). According to the changes in dietary behaviors, consumption of most nutrients, including dietary fiber, vitamin A, vitamin B group, vitamin C, and folate in the Worsened Group was significantly lower than in the No Change Group (p < 0.05). In conclusion, these results indicated that Chinese students practiced more unhealthy dietary behaviors after residing in Korea and Chinese students residing in Korea showed poor nutrient-based diet quality. Conduct of a follow-up study using blood profile tests is needed in order to assess the nutritional status of Chinese students. These results would be used in planning of a nutritional surveillance program for Chinese students.

Personal Information Overload and User Resistance in the Big Data Age (빅데이터 시대의 개인정보 과잉이 사용자 저항에 미치는 영향)

  • Lee, Hwansoo;Lim, Dongwon;Zo, Hangjung
    • Journal of Intelligence and Information Systems
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    • v.19 no.1
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    • pp.125-139
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    • 2013
  • Big data refers to the data that cannot be processes with conventional contemporary data technologies. As smart devices and social network services produces vast amount of data, big data attracts much attention from researchers. There are strong demands form governments and industries for bib data as it can create new values by drawing business insights from data. Since various new technologies to process big data introduced, academic communities also show much interest to the big data domain. A notable advance related to the big data technology has been in various fields. Big data technology makes it possible to access, collect, and save individual's personal data. These technologies enable the analysis of huge amounts of data with lower cost and less time, which is impossible to achieve with traditional methods. It even detects personal information that people do not want to open. Therefore, people using information technology such as the Internet or online services have some level of privacy concerns, and such feelings can hinder continued use of information systems. For example, SNS offers various benefits, but users are sometimes highly exposed to privacy intrusions because they write too much personal information on it. Even though users post their personal information on the Internet by themselves, the data sometimes is not under control of the users. Once the private data is posed on the Internet, it can be transferred to anywhere by a few clicks, and can be abused to create fake identity. In this way, privacy intrusion happens. This study aims to investigate how perceived personal information overload in SNS affects user's risk perception and information privacy concerns. Also, it examines the relationship between the concerns and user resistance behavior. A survey approach and structural equation modeling method are employed for data collection and analysis. This study contributes meaningful insights for academic researchers and policy makers who are planning to develop guidelines for privacy protection. The study shows that information overload on the social network services can bring the significant increase of users' perceived level of privacy risks. In turn, the perceived privacy risks leads to the increased level of privacy concerns. IF privacy concerns increase, it can affect users to from a negative or resistant attitude toward system use. The resistance attitude may lead users to discontinue the use of social network services. Furthermore, information overload is mediated by perceived risks to affect privacy concerns rather than has direct influence on perceived risk. It implies that resistance to the system use can be diminished by reducing perceived risks of users. Given that users' resistant behavior become salient when they have high privacy concerns, the measures to alleviate users' privacy concerns should be conceived. This study makes academic contribution of integrating traditional information overload theory and user resistance theory to investigate perceived privacy concerns in current IS contexts. There is little big data research which examined the technology with empirical and behavioral approach, as the research topic has just emerged. It also makes practical contributions. Information overload connects to the increased level of perceived privacy risks, and discontinued use of the information system. To keep users from departing the system, organizations should develop a system in which private data is controlled and managed with ease. This study suggests that actions to lower the level of perceived risks and privacy concerns should be taken for information systems continuance.

Temporal Variations of Sea Water Environment and Nutrients in the East Coast of Korea in 2013~2017: Sokcho, Jukbyeon and Gampo Coastal Areas (2013~2017년 동해 연안의 해양환경과 영양염의 시간적 변동 : 속초, 죽변, 감포 연안)

  • Kwon, Kee-Young;Shim, Jeong Hee;Shim, Jeong-Min
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.4
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    • pp.457-467
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    • 2019
  • To investigate the long-term variation characteristics of nutrients in the east coast of Korea, water temperature, salinity, dissolved oxygen, and nutrients were measured at three stations of Sokcho, Jukbyeon and Gampo coasts for five years from 2013 to 2017. For five years, the water temperature of the East Sea coast was in the range of $1.2{\sim}28.8^{\circ}C$, the salinity was in the range of 30.63~34.79 and the dissolved oxygen (DO) was in the range of 3.53~7.64 mL/L. Distribution and variation of the water environment factors in the study area were determined by the vertical stratification of water column and distribution of water temperature. The high DO concentration in Sokcho coast From 2015 to August 2016 is presumed to be the result of the southward inflow of North Korean Cold Water (NKCW). Concentrations of dissolved inorganic nitrogen (DIN, $NH_4-N+NO_2-N+NO_3-N$) ranged $0.11{\sim}24.19{\mu}M$, phosphate concentration ranged $0.01{\sim}1.75{\mu}M$, and silicate ranged $0.17{\sim}32.80{\mu}M$. The N:P ratio was in the range of 0.7~54.3 (mean 15.2) and the N:P slope was in the range of 11.67~13.75. The N:P ratios in this study were lower than the Redfield ratio (16), indicating that nitrate did act as a limiting factor in phytoplankton growth. The correlation ($R^2$) of total N:P ratio was as high as 0.95, indicating that the effect of the surrounding land or non-point sources was not significant. In conclusion, the spatial and temporal variation of nutrients in the east coast of Korea was determined by the vertical mixing of water mass with thermocline and mainly affected by physical factors such as influx of external water masses and coastal upwelling, and the influences from inflows from the land were minimal.

Analysis of Thermal Environment Improving Effects of Green Curtain in Summer (Green Curtain 형식의 벽면녹화시스템을 통한 여름철 건물 실내 열환경 비교 분석)

  • Lee, Sunyoung;Jo, Sangman;Park, Sookuk
    • Journal of the Korean Institute of Landscape Architecture
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    • v.50 no.5
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    • pp.80-89
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    • 2022
  • In order to solve the limitations of horizontal thermal environment improvement, this study compared the thermal environment of the indoor and outdoor of a building in summer according to the presence or absence of a green curtain, a vertical greening method. In the summer of 2021, the air temperature, relative humidity, wind speed, and shortwave and longwave radiation were measured at a central point inside a building and the grass field outside of the building to determine the human thermal sensation index, PET and UTCI. As a result, the green curtain showed an average 1.6℃ cooler air temperature during the daytime, but it did not have an effect at night. For relative humidity, it showed higher humidity indoors by an average of 5.6% and 1.0% during the daytime and at night, respectively. Wind speed was 1.4-1.8 ms-1 and 1.4-1.5 ms-1 higher outdoors on average during the daytime and at night, respectively, showing a high value outdoors regardless of whether a green curtain was installed. The green curtain showed an average indoor mean radiant temperature reduction effect of 4.7℃ during the daytime, but it did not have an effect at night. In PET and UTCI, the green curtain reduced the indoor PET by about a 1/3 level, an average of 2.1℃, and the indoor UTCI by about a 1/6 level, an average of 1.1℃, during the daytime. However, no effects appeared in PET and UTCI at night. For landscape planning, a green curtain can effectively modify the thermal environment during the daytime in summer.

Development of Rapid-cycling Brassica rapa Plant Program based on Cognitive Apprenticeship Model and its Application Effects (인지적 도제 모델 기반의 Rapid-cycling Brassica rapa 식물 프로그램의 개발 및 적용 효과)

  • Jae Kwon Kim;Sung-Ha Kim
    • Journal of Science Education
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    • v.47 no.2
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    • pp.192-210
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    • 2023
  • This study was intended to develop the plant molecular biology experimental program using Rapid-cycling Brassica rapa (RcBr) based on the teaching steps and teaching methods of the cognitive apprenticeship model and to determine its application effects. In order to improve a subject's cognitive function and expertise on molecular biology experiments, two themes composed of a total 8 class sessions were selected: 'Identification of DFR gene in purple RcBr and non-purple RcBr' and 'Identification of RcBr's genetic polymorphism site using the DNA profiling method'. Research subjects were 18 pre-service teaching majors in biology education of H University in Chungbuk, Korea. The effectiveness of the developed program was verified by analyzing the enhancement of 'cognitive function' related to the use of molecular biology knowledge and technology, and the enhancement of 'domain-general metacognitive abilities.' The effect of the developed program was also determined by analyzing the task flow diagram provided. The developed program was effective in improving the cognitive functions of the pre-service teachers on the use of knowledge and technology of molecular biology experiments. It was especially effective to improve the higher cognitive function of pre-service teachers who did not have the previous experience. The developed program also showed a significant improvement in the task of metacognitive knowledge and in the planning, checking, and evaluation of metacognitive regulation, which are sub-elements of domain-general metacognitive abilities. It was found that the developed program's self-test activity could help the pre-service teachers to improve their metacognitive regulation. Therefore, this developed program turned out to be helpful for pre-service teachers to develop core competencies needed for molecular biology experimental classes. If the teaching and learning materials of the developed program could be reconstructed and applied to in-service teachers or high school students, it would be expected to improve their metacognitive abilities.