• Imaging Science in Dentistry
• /
• v.53 no.3
• /
• pp.269-269
• /
• 2023

• Journal of Microbiology and Biotechnology
• /
• v.30 no.4
• /
• pp.515-525
• /
• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• Journal of Engineering Education Research
• /
• v.21 no.2
• /
• pp.17-27
• /
• 2018

This study aims to investigate the effect of design thinking-based STEAM program for primary and secondary students. The design thinking-based STEAM program dealt with advanced scientific technology issues and it consisted of 8 topics. A questionnaire was developed to explore the change of Science concept awareness, Interest, Self-efficacy, Career consciousness of students through the program. The questionnaire consisted of following 4 areas, such as 'Science concept awareness', 'Interest', 'Career consciousness' and 'Self efficacy'. Pre-post test was conducted to 75 primary and secondary students. As a result of analyzing post-test compared to pre-test, 'Science concept awareness', 'Career consciousness' and 'Self efficacy' of students were improved. And the 'Interest' wasn't changed significantly. Through this research, it can be suggested that the design thinking-based STEAM program can be useful at school education to improve the Science concept awareness, Career consciousness and Self-efficacy of primary and secondary students. It is also proposed that further research of the various areas of interest and other affective variables will be needed.

• Journal of Gifted/Talented Education
• /
• v.18 no.3
• /
• pp.497-518
• /
• 2008

This study aims to examine how Science Gifted Education Center(SGEC) affect the decision of graduates of gifted education institute in applying for science-related Special high school. To this end, the study investigated factors that affected their decision on applying for higher SGEC, such as career goal prior to their admission to a SGEC, motivation for the admission, and curricular activities at SGEC. A questionnaire survey was administered on 30 students, and resulting data were used as basic information to construct interview questions. Selected 22 students among them were interviewed separately, with transcripts of the recorded interviews being analyzed. The results of the study indicate that among many factors of SGEC for the gifted which affected student's choice of special-purpose high school of science, activities of the center putting an emphasis on open research activities and social interaction had a positive effect on the students' choice of special high school.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
• /
• 2002.11a
• /
• pp.25-28
• /
• 2002

In this paper, a micromachined millimeter-wave end-gap band-pass filter (BPF) is presented. The millimeter-wave BPF is designed using 3D design software, Zeland IE3D with the center frequency of 60 GHz, band width of 3 GHz, ripple of 1㏈ and insertion loss of 2.5dB. This type of micromachined BPF can be used in millimeter-wave circuit.

• Proceedings of the Polymer Society of Korea Conference
• /
• 2006.10a
• /
• pp.334-334
• /
• 2006

Helical polymers like polyisocyanates with single screw sense are essential to exhibit sophisticated functions like molecular recognition, self-replication, chirality memory and catalytic activity. One approach that has not been explored is the effect on handedness of the polyisocyanates through end-capping with a chiral residue. Induction of chirality in poly(n-hexyl isocyanate) was studied by end-capping with chiral (R and S) 2-bromo-3-methylbutyryl chloride(R-BMBC and S-BMBC). We have shown that a control over living anionic polymerization of HIC by using a suitable initiator affords an opportunity to introduce chiral end-groups with 100% yield and in high purity. This has resulted in helicity induction through extended lengths several orders of magnitude.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.6
• /
• pp.1115-1123
• /
• 2016

_L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the _L-asparaginase gene (_L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of _L-ASPG86 (_L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The _L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant _L-asparaginase (r-_L-ASPG86) showed optimum conditions at 37-40℃, pH 9. Moreover, r-_L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-_L-ASPG86 was 687.1 units/mg under optimum conditions (37℃, pH 9, and 5 mM MnSO₄).

• Journal of Science Education
• /
• v.38 no.1
• /
• pp.205-229
• /
• 2014

The purposes of this study were to explore what components of science communication and its level are contained in text panels of exhibitions in natural history museums and to propose its development direction of exhibition education in Korea. First, to find out the component and level of science communication contained in exhibition panels, the researcher team developed the analyzing tool which was called SEPAT (Science Exhibition Panel Analyzing Tool), then employed them to profile the component and level of science communication. Second, the researchers introduced the exemplary designed media of exhibitions to demonstrate how much science communication could be enhanced. The results were made as follows. First, the components of science communication was considerably weighted toward to 'concept' one. There were also a few 'awareness' and 'engagement', both of which were under 5% in each zone of exhibition and there was not 'NOSI' or 'opinion' embedded in the analyzed exhibitions. Second, the various type of designing exhibitions were found to promote or enhance the restrictedly represented components of science communication. It is suggestable for exhibitions to be designed through various type of 'media' to enhance science communication. Visitors are required to experience rich science communication to meet their educational needs, and exhibition developers in natural history museums and other museums are recommended to be professional in containing all components of science communication through various type of designing exhibitions.

• Journal of The Korean Association For Science Education
• /
• v.32 no.4
• /
• pp.751-759
• /
• 2012

This study examined the perception of secondary gifted students of a university-affiliated science-gifted education center toward its educational programs. This study surveyed 184 students who are enrolled in the secondary gifted educational programs of a university-affiliated science-gifted education center. The results showed that the students are generally satisfied with the gifted educational programs. The students also said that the programs have provided more knowledge to them and improved their creativity. It is significant to examine how students currently participating in gifted educational programs perceive the programs and how they are influenced by the programs. Therefore, improvements required for the development and operation of future gifted educational programs should be drawn based on the collection of the results of perception surveys from each gifted education center.

• Journal of The Korean Association For Science Education
• /
• v.5 no.2
• /
• pp.139-145
• /
• 1985

This study was undertaken to analyze the research trends on science education in Korea. In this study the analysis for of trends of researches on science education, nine areas such as historical change of science Education, Processes of science learning science curriculum, science instruction, teaching-learning materials and equipment for science education, valuation on science education, survey on Korean science education, policy and management of science education, and natural science, were chosen for the analysir. All science education. thesis and dissertations in Korea, papers of science education published by the science center of the Seoul National University and the papers of the Journal of the Korean Association of Res Search in science Education were analyzed. The findings of this study are as follows: 1. Seventy percentile of science educational thesis and dissertations are on natural science areas. 2. About 14% of all papers being sampled is in science curriculum research category. There are few research studies on historical changes of science education, and teaching-learning materials and equipments for science education.