• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.531-541
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• 2014

This research investigated the reproduction cycle, litter size, and the effects of factors of red-tongue viper snake inhabiting in Jeju Island, to delve into their life strategy. Field survey was conducted in Jeju Island from May 2006 to November 2008. Reproduction cycle was analyzed through measurements of testis and follicle sizes in laboratory from March 2009 to December 2010. According to the research results, the sizes of red-tongue viper snake's testis and follicle clearly changed seasonally. The number of eggs within the oviduct were greater on the right side ($2.6{\pm}1.0$ eggs, n=16) than on the left side ($1.8{\pm}0.5$ eggs, n=16) (t=-2,721, p<0.05). Average (${\pm}SD$) of survival litter size (SLS) was $4.4{\pm}1.7$ (1~9, range), while total litter size (TLS) was $4.7{\pm}1.5$ (3~9, range), which were not statistically significant. However, their litter sizes were similar to the number of eggs within the oviduct (t=0.039, P>0.05). Relative litter mass (RCM) was $0.42{\pm}0.13$ (0.18~0.79, n=33), and tended to increase, as maternal condition of pre-parturition (MCPPI) was getting better. The sexual ratio of delivered litters showed no significant difference between male and female red-tongue viper snakes (♂:♀ = 1.15:1, n=73 ; ${\chi}^2$=0.342, P>0.5). Average neonate mass showed a weak correlation with maternal mass of pre-parturition (MMPP1) (r=0.387, P<0.05, n=33). Average neonate Snout-vent length (SVL) also demonstrated a weak correlations with maternal SVL (r=0.399, P<0.05, n=33) and MMPP1 (r=0.344, P<0.05, n=33). Average neonate mass and maternal SVL approached significant probability (r=0.323, P=0.067, n=33). This indicates that mother snakes can bear bigger litter due to its larger size. In some cases, litter's weight decreases as mother snakes are bearing more litter; however, the red-tongued viper snake did not show such exchange relationship. From this, it can be conjectured that a red-tongued viper snake has peculiarity of its own species. The research results are predicted to be used as the basis to find a life history of red-tongued viper snake.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.157-163
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• 2000

In a previous report, 50 potato cultivars were screened for infestation levels by the coppery click beetle (Selatosomus puncticollis Motschulsky) in the field. Subsequently, we selected 10 potato cultivars (Anco, Atlantic, Bintje, Dejima, Denali, Jopung, Irish Cobbler, Namsuh, Shepody, and Superior) to evaluate feeding preferences by wireworms, and to analyze some factors associated with resistance. The injury rates and number of holes in potato tubers damaged by larvae of S. puncticollis were checked in the field and laboratory. Additionally, some of their chemical characters (contents of glycoalkaloids, total-nitrogen, Ca, K, Mg, sugars, and starch) were quantified. And finally correlation analysis was conducted to see whether there is a possible relationship between these characteristics and the damage level. The tuber injury rates by S. puncticollis larvae were generally high showing 19% to 73% of damage level. The highest number of tuber hole damaged by S. puncticollis larvae was found on cv. Namsuh, but generally fewer on cvs. Anco, Atlantic, Bintje, Denali and Superior. No activities for $\alpha$-chaconine, $\alpha$-solanine and $\alpha$-tomatine at a concentration of 2,500 ppm were found to S. puncticollis larvae. The contents of glycoalkaloids in tuber were different depending on cultivars. In tubers, cv. Superior contained the highest level of 18.8 mg%, but cv. Irish Cobbler had the lowest level of 6.39 mg%. Concentrations of reducing sugars and total free sugars in tubers of cv. Namsuh were 0.71 % and 2.95%, but 0.26% and 1.77% in those of cv. Dejima, respectively. For the content of potato starch, cvs. Bintje, Dejima and Irish Cobbler showed higher level, but cvs. Jopung and Shepody lower. The highest contents of total nitrogen, Ca, K and Mg in tuber were found on cvs. Anco, Atlantic, Anco and Jopung, respectively. From correlation analyses, injury rate by S. puncticollis larvae was correlated with total nitrogen content (r = -0.71435*) and total sugar content in tuber (r = 0.78018*). Such information will become essential in developing integrated pest management programs and also in breeding new potato cultivars resistant to the wireworms.

• Journal of Food Hygiene and Safety
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• v.21 no.2
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• pp.118-128
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• 2006

This study was conducted to determination of the endocrine distruptor function of 'Parabens' by dosing ethyl paraben, propyl paraben, isopropyl paraben, butyl paraben and isobutyl paraben to the immature SD rats. 18 groups were given vehicle control group, negative control group (Dibutyl phthalate), postive control group ($1'7-{\alpha}$ Ethynylestrdiol) and each paraben groups involved 3 dose level. Rats were injected with 62.5, 250 and 1,000mg/kg from postnatal day 19 till 21 once a day in subcutaneous and a total 3, times. There was no treatment related death. but, subcutaneous nodule, edema, alopecia and scrub formation on injection site was observed. These signs was become worse in high dose level. these signs was cause from physical stimulation by test substance which parabens were mix with com oil as vehicle. In the analysis of organ weights, absolute and relative weights of brain, spleen, liver, thymus, heart, kidneys, adrenals, ovaries and vagina were no difference with control group. but, wet and blotted weight of uterus was increased in every high dose parabens treat group. Especially, all dose level of isobutyl paraben was showed increment of uterus weight. uterus dilatation of parabens treated group was observed in gross anatomic pathology and these result was agree with wet and blotted weight of uterus. In the result of this study, estrogenic effect as endocrine distruptor was observed in ethyl paraben, propyl paraben, isopropyl paraben, butyl paraben and isobutyl paraben. and it was considered isobutyl paraben has highest estrogrnic effect under the condition of this study.

• Journal of Korean Academy of Nursing
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• v.4 no.3
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• pp.63-79
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• 1974

1. The purpose of this study. The purpose of this study is to plan and investigate short-term nursing education of two-years associate degree program to produce middle-level professional nurses which are needed by-society and nation. Current nursing education in Korea is divided into four years degree program, three years diploma program Even (though) there are differences in the aims of their education, the curriculums are not much different between the education for producing leaders which is its basic purpose and training middle-level professional nurses. Therefore the purpose of associate degree program lies in minimizing the waste of time and finance which are invested for long-term education for middle-level professional nurses. And also this coincide with the policy of national technical training and definite supply of nurse manpower according to health policy for effective role and ability of nurse. 2. The method of study. This is based on the study of literature, research on the actual condition and investigation of opinion- through questionnaire. L) The study of literature: Domestic and foreign literatures for two years associate degree program were studied and investigated. 2) Research on the actual condition : Current three years nursing education program was collected and analysed. 3) Investigation of opinion. The problem of curried nursing education system and the possibility of two years associate degree program were investigated through questionnaire. 3. The result of the study. 1) The trend of recent nursing education. a. The aims of nursing in past chiefly taking care of physical disease of patient has recently changed to nursing of character including physical, mental, socio-economic, educational and psychological condition. b. For the performing systematic and effective nurse's duty according to her role, the-change of educational system which is classified as a range of education the period of education and certificate after graduation has been enforced or fulfilled. c. Nursing education also has a trend to become a collage or two years associate degree program which can get same legal protection as other educational institutions whose basic purpose is education. Attached nursing school to hospital is getting disappeared because of disadvantage of educational system. 2) Problems. Depending upon research on actual condition of current 3 years nursing education program. a. There are too many subjects. b. Contents of education could be doubled because major subjects are subdivided in detail. c. The credits for graduation are too heavy comparing to the period of study or the ability of students. (The necessary credits are 150.8 for three years according to actual investigation 4. There is no certain standard in organizing curriculum therefore there are too much differences between schools. 4. Basic Plan. The plan for two years associate degree program in nursing education depending on demand of professional nursing field of society is based on following items. 1) Training middle-level professional nurse lay emphasis on liberal arts and basic major field. 2) Liberal arts are divided into required and optional subjects and students could take courses by choice. 3) Major subjects are compound together by fields and they become the sciences of nursing Ⅰ,Ⅱ,Ⅲ,Ⅳ and every items has its educational purposes and contents major study includes laboratory practice and clinical experience. 4) The required credits for graduation are to which means 17-18 credits a semester. The above has been planned to solve the problems of current three years nursing education program. In conclusion for the achievement of this system, following items are needed. 1) It is necessary to change educational administration and system such as amendment of educational law or order of educational application of law. 2) Qualified professors should be available to understand and develope the idea or purpose of this educational system. 3) Local medical institutions should be opened widely and educational for clinical training. 4) The job after graduation should he secured positively.

• Journal of Aquaculture
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• v.8 no.4
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• pp.261-269
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• 1995

A practical control diet was prepared and compared with raw fish ($80\%$ frozen horse mackerel + $20\%$ commercial binder meal) and moist pellet ($50\%$ frozen horse mackerel + $50\%$commercial binder meal) diets to develop a practical diet formulation for Korean rockfish (Sebastes schlegeli). Korean rockfish averaging 68.5g were fed with the three experimental diets for 11 months. Final average body weight was approximately 330g and showed no significant difference between fish groups (P>0.05). Feed efficiency of fish from control diet was significantly lower than those of fish from raw fish and moist pellet diets (P<0.05). Protein and lipid retention efficiency of fish ranged $26.9-28.8\%$ and $86.5-110.2\%$, respectively, without any significant difference between the different diets. At the end of the experiment, chemical composition for the various parts of fish body and flesh quality of the raw fish were examined. The data showed that there was no significant difference between fish from the different diet groups for moisture, protein and lipid contents of whole body, liver, and dorsal muscle. Also, thirty three panelists did not find any difference in color, smell, texture, and taste of the raw fish flesh. These results suggest that Korean rockfish can be cultured with a laboratory practical feed without any negative effects on growth, nutrient utilization, chemical composition of fish, and quality of raw fish. Therefore, the next step in developing practical feed is to modify the control diet for improving fish performance and reducing feed cost.

• The Journal of Engineering Geology
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• v.22 no.1
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• pp.123-134
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• 2012

Hydrochemical analyses, carbon isotopic (${\delta}^{13}C_{DIC}$) analyses, and noble gas isotopic ($^3He/^4He$ and $^4He/^{20}Ne$) analyses of the Dalki carbonate waters in the Chungsong area were carried out to elucidate their hydrochemical composition and to determine the source of $CO_2$ gas and noble gases. The carbonate waters have a pH of between 5.93 and 6.33, and an electrical conductivity 1950 to $3030{\mu}S/cm$. The chemical composition of all carbonate waters was Ca(Mg)-$HCO_3$, with a high Na content. The contents of Fe, Mn, and As in some carbonate waters exceed the limit stipulated for drinking water. The concentrations of major ions are slightly higher than those reported previously. The ${\delta}^{13}C_{DIC}$ values range from -6.70‰ to -4.47‰, indicating that the carbon originated from a deep-seated source. The $^3He/^4He$ and $^4He/^{20}Ne$ ratios vary from $7.67{\times}10^{-6}$ to $8.38{\times}10^{-6}$ and from 21.32 to 725.7, respectively. On the $^3He/^4He$ versus $^4He/^{20}Ne$ diagram, the noble gas isotope ratios plot in the field of a deep-seated source, such as mantle or magma. We therefore conclude that $CO_2$ gas and noble gas in the Dalki carbonate waters originated from a deep-seated source, rather than an inorganic $CO_2$ origin as suggested in a previous study.

• Journal of the Korean Society of Food Culture
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• v.6 no.2
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• pp.215-222
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• 1991

The difference between Kimchi, Japanese pickles, Korean Salted and fermented fish intestines and Japanese Salted and fermented fish intestines was investigated by comparising composition of inorganic cation in them. A high-performance liqid chromatography was used for the determination of Na, K, Ca and Mg in Kimchi, Japanese pickles and Salted and fermented fish intestines. The Kimchi samples analyzed were produced in the home, in the restaurant, in the nunnery and by food manufactures in Korea, and the pickles used were produced by food manufactures in Japan, and Salted and fermented fish intestines used were produced by food manufactures in Korea or Japan. The results obtained were summarized as following: (1) Sodium chloride in Kimchi was $1.8{\pm}0.37%$, $2.8{\pm}0.60%$ in Asazuke, Japanese picles which the fresh vegitables was seasoned with sodium chloride and seasoning, $1.8{\pm}0.32%$ in Japanese Kimchi, Japanese pickles which the fresh vegitables was seasoned with the mixture of sodium chloride, garlic powder, red peper's powder and seasoning, $5.3{\pm}0.66%$ in Shoyu Zuke, Japanese pickles which the salted vegetables was seasoned with sodium chloride and seasoning after desalting. (2) Na/K value in Kimchi was $1.7{\pm}0.17$ and $4.6{\pm}1.44$ in Asazuke, $2.6{\pm}0.85$ in Japanese Kimchi and $27.3{\pm}6.79$ in Shoyu zuke respectively. (3) Kimchi contained more K and Ca than Japanese pickles. (4) Kimchi remarkably contained the Ca originating to salted and fermented fish intestines. As the results of this study, it was presumed that while Kimchi and Japanese pickles is well known as a food that contributed to high blood pressure, Kimchi and Japanese Kimchi might not cause the high blood pressure because they have an excellent balance of Na/K value and low Na content. And also it was considered that these differences might be caused by the difference of th food taste between and Korean and Japanese people.

• Biomedical Science Letters
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• v.4 no.1
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• pp.65-72
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• 1998

Recently there have been frequent reports on human infection caused by the Diphyllobothridae in Korea. The adequate opportunities for Koreans to eat raw fish, the primary infection medium of cestodes and the human infection through drinking water by cyclops, the first intermediate host are believed to be main reasons for the infection. The first task of this study was to classify and diagnose the species by differentiating morphological characteristic between scolex and proglottids of cestodes. However, the initially available diagnosis was done with the patient's symptoms and the eggs obtained from his stool. It is important to differentiate the species by the eggs of Diphyllobothrium latum especially in that it can help get advance information for a more reliable analysis in the near future. The morphological and diagnostic results from proglottids and eggs of Diphyllobothrium latum, Diphyllobothrium latum parvum and Spirometra erinacei are as follows； In each kind of cestodes from the patient's stool, the shape and size of 50 eggs were measured. Eggs of Diphyllobothrium latum had an operculum and were ovoidal or ellipsoid to elliptical in shape. Eggs of Diphyllobothrium latum parvum were more ovoidal in shape and smaller in size than Diphyllobothrium latum. And eggs of Spirometra erinacei were asymmetrical in width and long and slender in shape. The average lengths and widths of Diphyllobothrium latum, Diphyllobothrium latum parvum and Spirometra erinacei were 61.4$\times$41.7 $\mu\textrm{m}$, 55.9$\times$41.4 $\mu\textrm{m}$ and 66.7$\times$36.4 $\mu\textrm{m}$, respectively. After the segments of each cestode were fixed, embedding and hematoxylin-eosin dyeing on a microtome-made specimen were done. The micrographs of the semicon's aceto-carmine dyed specimen showed that Diphyllobothrium latum and Diphyllobothrium latum parvum had a centrally-located genital gland and an opened uterine pore. The yolks were observed on both sides of proglottids and had a typical rosette pattern. Yet, Diphyllobothrium latum parvum was shown smaller than Diphyllobothrium latum in the micrograph. Proglottids of Spirometra erinacei displayed that the uterus was rolled spirally more than five to seven times, and connected successively to the seminal vesicle in the cirrus sac. Shown above, this study was performed to measure the size of eggs and analyze the morphological characteristics of proglottids and provided the measurements of three types of cestodes obtained by a light microscope.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.17 no.1
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• pp.53-62
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• 2007

목적: 1990년대 초반부터 시작된 우리나라의 산업 보건에 대한 사회적 관심의 증대와 시장의 요구에 따라 산업위생기관의 양적인 팽창이 이루어지긴 하였으나 실험실 관련 안전보건규정이 미비한 상태에서 추진되어 산업위생 실험실에서 다양한 유해 위험성 인자를 취급함에도 불구하고 사용되는 량이 소규모로 인해 안전보건과 관련된 사항은 대부분 간과하고 있으며, 산업위생 실험실의 안전보건관리 실태는 선진외국에 비해 상당히 낙후되어 있는 실정이다. 따라서 본 연구는 우리나라 산업위생 실험실과 관련한 안전보건관리 실태를 평가함으로써 향후 실험실의 안전보건관리 수준을 향상 시키는데 효과적인 자료로 활용하는데 기여하고자 한다. 방법: 산업위생실험실의 안전보건 실태를 파악하기 위해 설문조사를 수행하였으며, 설문은 실험실의 전반적인 안전보건, 화학물질 저장 및 용기, 가연성 및 인화성 화학물질, 가스 실린더, 의사전달, 응급처치 및 비상사태 장비, 정리정돈, 소방안전, 전기안전, 개인보호구, 흄후드 및 환기, 및 이황화탄소 취급 사례로서 12개 영역으로 구성되었다. 연구대상은 2001년 한국산업안전공단 정도관리프로그램에 참여한 기관으로서 총 대상은 119개 기관이었다. 연구기간은 2002년 7월 01일부터 8월 30일까지 약 60일 이었다. 설문은 반송봉투에 넣어 설문완성 후 연구자에게 보낼 수 있도록 배려하였으며, 1차 설문을 보낸 후 2주 후에 설문 참여를 독려하기 위해 엽서를 발송하였고, 다시 2주 후에 각 산업위생기관에 개별 전화 연락을 취하였다. 그 결과 63% (75개 기관)의 완성된 설문을 얻을 수 있었다. 설문의 총 조사항목은 79문항으로 구성되어 있으며, 산업위생실험실의 안전보건 수준을 정량적으로 평가하기 위해 안전보건 매뉴얼, 교육훈련프로그램 및 한국산업안전공단 실험실 지침서를 보유한 기관과 그렇지 않은 기관간의 차이는 선별된 67문항에 대해 각 문항에 합당하거나 적절할 경우 1점을 부여하는 방식으로 점수화하였다. 이들의 관련성을 파악하기 위하여 Microsoft-Excel 2000 프로그램을 이용하여 two-tailed t-test 분석을 실시하였다. 결과 1. 산업위생실험실 운영과 관련한 67개 항목(항목별로 각 1점 부여)에 대한 안전보건 성과지수화 (100점으로 점수 환산) 결과 안전보건 매뉴얼을 보유한 기관은 $42.98{\pm}13.36$(p<0.001)점, 교육훈련프로그램을 보유한 기관은 $50.75{\pm}14.12$(p<0.01)점, 한국산업안전공단 실험실 지침서를 보유한 기관은 $43.58{\pm}11.92$(p<0.01)점으로 그렇지 않은 기관에 비해 통계적으로 유의하게 높은 점수분포를 보였다. 2. 화학물질 보관 캐비닛이 있는 기관 중 64.8%의 기관은 화학물질을 알파벳 순으로, 27.8%는 분류 기준에 따라, 그리고 7.4%는 무작위로 보관하고 있었다. 3. 단지 8.0% (6개)의 기관만이 실험실내에 눈세정 분수(2 개), 샤워기(3개) 및 눈세정물병(3개, 2개 중복 응답 기관)과 같은 응급처치 장치 및 물품을 갖추고 있었다. 4. 89.0%의 기관이 흄후드내에 화학물질을 보관하고 있었다. 5. 물질안전보건자료, 흄후드 기록지, 비상사태 절차서 및 한국산업안전공단 실험실 지침서와 같은 문서관리는 부적절하게 관리 및 기록되고 있었다. 6. 대부분의 산업위생 실험실은 응급처치 장비, 화학물질 또는 가스용기 보관실과 같은 실험실 안전설비가 부족할 뿐만 아니라 정리정돈, 화학물질 저장 캐비닛, 안전보호구 및 흄후드와 같은 관리가 미비하였다. 결론: 이상의 결과에 근거하여 기관장은 실험실의 안전보건관리를 위하여 문서관리체계를 제공하고, 모든 실험실 종사자가 적절한 개인보호구를 착용할 수 있도록 하며, 비상 장비를 설치하고, 실험실과 관련한 적절한 규정을 제정하며, 교육훈련 프로그램을 제공하여야 한다. 또한 실험실 종사자는 실험 중 적절한 개인보호구의 착용하고, 비상장비 이용에 대한 교육훈련에 참여하며, 적절한 실험실 운영관리에 대한 책임감을 가져야 한다. 위의 권고사항 이행을 위해서 KOSHA는 KQCP 프로그램에 실험실 안전보건관리 항목을 삽입하여 주기적인 평가를 수행할 필요가 있다. 우리나라 실험실의 특성을 고려할 때 본 연구결과는 일반 실험실에도 적용될 수 있을 것으로 생각된다.

• Journal of Soil and Groundwater Environment
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• v.11 no.1
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• pp.14-22
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• 2006

Laboratory column experiment for simultaneous removal of Cd and Cr(VI) were conducted using newly developed material of Fe-loaded zeolite having both reduction ability and sorption capacity. The solution containing Cd and Cr(VI) was injected into the column and the breakthrough curves (BTCs) for the contaminants were observed at the effluent port. Cd breakthrough was not initialized until Cr(VI) breakthrough was completed. Therefore it could be concluded that overall efficiency of Fe-loaded zeolite should be determined by the reactivity for Cr(VI). The relative concentration of Cr(VI) BTC increased to the unit value while initial breakthrough was delayed and the propagation of breakthrough was slowed. In order to quantitatively describe the shape of Cr(VI) BTC, new parameters of ${\alpha}\;and\;{\beta}$ designated to be shape parameters, were defined and applied in contaminant transport concentration. These parameters were employed to represent the degree of initial breakthrough delay and the degree of breakthrough propagation, respectively. As initial contaminant concentration increased, ${\alpha}$ decreased, which indicated the delay of BTC's initiation. And as initial contaminant flow rate increased, ${\beta}$ decreased, which represented the faster propagation of the BTC. From these results, Fe-loaded zeolite was found to be an effective reactive material for PRBs against heavy metals having different ionic forms in groundwater. And it could be expected that as groundwater flows faster, the propagation of breakthrough would be faster and as contaminant concentration is higher, the initial point of breakthrough would appear earlier.