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Oestrogenic Activity of Parabens in Uterotrophic Assay  

Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung Ji-Youn (Department of Companion and laboratory Animal Science, Kongju National University)
Park Cheol-Beom (Biotoxtech Co. Ltd.)
Park Sun-Hee (Biotoxtech Co. Ltd.)
Shim Yhong-Hee (Biotoxtech Co. Ltd.)
Kang Dae-Hyuck (Biotoxtech Co. Ltd.)
Lee Sung-Ho (Biotoxtech Co. Ltd.)
Yu Jong-Hoon (Biotoxtech Co. Ltd.)
Kim Sun-Jung (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Lee Sung-Hoon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Park Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Lee Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Kang Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Food Hygiene and Safety / v.21, no.2, 2006 , pp. 118-128 More about this Journal
Abstract
This study was conducted to determination of the endocrine distruptor function of 'Parabens' by dosing ethyl paraben, propyl paraben, isopropyl paraben, butyl paraben and isobutyl paraben to the immature SD rats. 18 groups were given vehicle control group, negative control group (Dibutyl phthalate), postive control group ($1 Ethynylestrdiol) and each paraben groups involved 3 dose level. Rats were injected with 62.5, 250 and 1,000mg/kg from postnatal day 19 till 21 once a day in subcutaneous and a total 3, times. There was no treatment related death. but, subcutaneous nodule, edema, alopecia and scrub formation on injection site was observed. These signs was become worse in high dose level. these signs was cause from physical stimulation by test substance which parabens were mix with com oil as vehicle. In the analysis of organ weights, absolute and relative weights of brain, spleen, liver, thymus, heart, kidneys, adrenals, ovaries and vagina were no difference with control group. but, wet and blotted weight of uterus was increased in every high dose parabens treat group. Especially, all dose level of isobutyl paraben was showed increment of uterus weight. uterus dilatation of parabens treated group was observed in gross anatomic pathology and these result was agree with wet and blotted weight of uterus. In the result of this study, estrogenic effect as endocrine distruptor was observed in ethyl paraben, propyl paraben, isopropyl paraben, butyl paraben and isobutyl paraben. and it was considered isobutyl paraben has highest estrogrnic effect under the condition of this study.
Keywords
Parabens; Estrogenic effect; Uterotrophic assay; Endocrine disrupter;
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1 Christian M.S., Hobennan A.M., Bachmann S. and Hellwig J. : Variability in the uterotrophic response assay (an in vivo estrogenic response assay) in untreated control and positive control (DES-DP, 2.5 microG, bid) wistar and Sprague Dawely rats. Drug chem. Toxicol. 1, 51-100 (1998)
2 Thigpen J.E., Haseman J.K' Saunders H., Locklear J., Caviness G, Grant M. and Forsythe D. : Dietary factors affecting uterine weights of immature CD-1 mice used in uterotrophic bioassays. Cancer Detection and Prevention 26, 381-393 (2002)   DOI   ScienceOn
3 Kim H.S., Shin lH., Moon H.J., Kang LH., Kim T.S. Kim I.Y., Seok J.H., Pyo M.Y. and Han S.Y.. : Comparative estrogenic effects of p-nonylphenol by 3-day uterotrophic assay and female pubertal onset assay. Reproductive Toxicology 16, 259-268 (2002)   DOI   ScienceOn
4 Yamasaki K., Noda S., Imatanaka N. and Yakabe Y. : Comparative study of the uterotrophic potency of 14 chemicals in a utertrophic assay and their receptor-binding affinity. Toxicology Letters 146, 111-120 (2004)   DOI   ScienceOn
5 Gelbke H.P., Kayser M. and Poole A. : OECD test strategies and methods for endocrine disruptors. Toxicology 205(1-2), 17-25 (2004)   DOI   ScienceOn
6 Oshi: S. : Effect of propyl paraben on the male reproductive system. Food and Chemical Toxicology 40,1807-1813 (2002)   DOI   ScienceOn
7 Kang K.S., Kim H.S., Ryu D.Y, Che J.H. and Lee YS. : Immature uterotrophic assay is more sensitive than ovariectomized uteritrophoc assay for the detection of estrogenicity of pnonylphenol in Sprague -Dawely rats. Toxicology Letters 118, 109-115 (2002)   DOI   ScienceOn
8 Yamasaki K., Sawaki M., Noda S. and Takatuki M. : Effect of Olive, Com, Sesame or Peanut Oil in the Body Weights and Reproductive Organ Weights of Immature Male and Female Rats. Exp. Anim. 50(2), 173-177 (2001)   DOI   ScienceOn
9 Koda T., Umezu T., Kamata R., Morohoshi K., Ohta T. and Morita M. : Uterotrophic effects of benzophenone derivatives and a p-hydroxybenzoate used in ultraviolet screens. Environ.Res. 98(1), 40-45 (2005)   DOI   ScienceOn
10 Seidlova-Wuttke D., Jarry H. and Wuttke W. : Pure estrogenic effect ofbenzophenone-2 (BP2) but not of bisphenol A (BPA) and dibutylphtalate (DBP) in uterus, vagina and bone. Toxicology 205(1-2), 103-112 (2004)   DOI   ScienceOn