Excretory urography was performed on eight healthy minipigs to evaluate radiographic anatomical status and excretive function of the urinary tracts and to get an optimal procedure. The right and left kidneys were located in $T13{\sim}L4$and $T12{\sim}L3.5$, respectively. The left kidneys were located more cra-nially than the right. The everage lengths and widths of kidneys were $8.50{\pm}0.58$$(mean{\pm}SD)$and $4.30{\pm}0.39$ cm on the ventrodorsal projection and $8.70{\pm}0.76$, and $4.10{\pm}0.40$ cm on the lateral projection, respectively. The lengths of kidneys were twice the width. When the lengths of kidneys were com-pared with lengths of the second lumbar vertebras, the ratios of kidney lengths to L2 lengths were $3.62{\pm}0.30$ on the ventrodorsal projection and $3.63{\pm}1.10$ on the lateral projection. The lengths and 쟝손 of renal pelvis including major calices were $4.01{\pm}0.46$, and 2.20{\pm}0.41$ cm, respectively. The lengths and 쟝손 of minor calices were $0.49{\pm}0.06$, and $0.10{\pm}0.01$ cm. The lengths and widths of the ureters were $12.25{\pm}2.05$, and 2.94{\pm}0.86$ cm. The nephrogram stage was reached in 3{\sim}5$ minutes, and the pyelogram phase in $5{\sim}15$ minutes individually after injection of contrast medium. The result of this study shows similar information and procedure of excretory urography for other laboratory ani-mals and leads to the potential use of this method for minipigs.
Seasonal changes of biomass and cell size of bacteria and protozoa, and factors affecting their distribution in Lake Paldang and Kyungan Stream were analyzed from April to December, 2005. Bacterial abundance at Paldang Dam and Kyungan Stream was similar, but it did not much increase during hot summer period. Protozoan carbon biomass was much greater at Kyungan Stream compared to Paldang Dam. HNAN generally accounted for the majority of total protozoan biomass, but ciliates made up the highest proportion in April and November at Paldang Dam and June at both sites. PNAN showed low biomass at both sites, but it was high during spring and fall season. Small-sized HNAN ($3{\sim}7\;{\mu}m$) numerically predominated the protozoan community at both sites. Average cell size of HNAN was bigger at Kyungan Stream where nutrients concentration was much higher than Paldang Dam. Average cell size of ciliates varied seasonally; it was relatively small during the summer. HNAN biomass significantly correlated with Chl-a concentration and ciliates biomass at Paldang Dam, indicating that HNAN increase might link to the ciliates increase. At Kyungan Stream, HNAN biomass showed a significant relationship with PNAN biomass, and Chl-a concentration was closely related with both of HNAN and PNAN biomass. Ciliate biomass showed significant relationship with nutrient (TN, TP) and particulate matter (SS) only at Kyungan Stream. At both sites, protozoan biomass was significantly correlated with bacterial biomass, and ciliates were additionally related flagellates. High biomass of microbial components and the close relationships among them suggest that the energy transfer through the microbial loop may important in the plankton food web of Lake Paldang ecosystem.
Purpose: This study aims to evaluate and prospect for current research trend and developmental perspectives via analyzing recent biomaterial coated-implants study. Materials and methods: To investigate each subject respectively, several biomaterials that are using for implant surface coating were set as 'keywords'. By these keywords, major research groups in each subject were chosen, and research trend of them was analyzed. Trend of In vivo studies that examined selected biomaterials were analyzed to evaluate commercial potential. Results: The collagen research accounted for 40% of total implant study, which was the highest, and fibronectin, BMPs (bone morphogenetic proteins) and RGD (Arg-Gly-Asp) peptides followed, which were ranked in descending order. Furthermore, figures of all four research subjects were also increased with time, especially a sharp increase in RGD research. According to the results of major research group, collagen that was combined with other organic and inorganic biomaterials was mostly examined, rather than using collagen only. Major research groups investigating BMPs mostly focused on rhBMP-2. In animal studies, collagen was used as resorbable membrane in guided bone regeneration (GBR) or drug carrier, while BMPs were used with bone graft materials or coating material for titanium implant surface. Conclusion: There is not consistency of results even in identical subjects research field. Many studies are ongoing to optimize combination between mechanical surface treatment and biomaterials such as extracellular matrix component and growth factors.
The objectives of this study were to investigate the effect of applying three different chemical additives to the litter (rice hull) on broiler performance, ammonia and carbon dioxide gas reduction in a poultry house at 6 weeks. A total of 96 broiler chicks (6 treatments$\times$4 replicates$\times$4 birds) were fed the experimental diets for 6 weeks. The chemical additives were applies as a top dressing to the litter at a rate of 200 g ferrous sulfate $(FeSO_4)$, 200 g aluminum chloride $(AlCl_3)$ + 50 g calcium carbonate $(CaCO_3)$ and 20 g potassium permanganate $(KMnO_4)$ per kg litter, while the control group did not have the three different chemicals added to the litter. There were no significant differences in broiler performance between the three chemical additives and control group. $FeSO_4\;and\;AlCl_3\;+\;CaCO_3$ treatment reduced ammonia production from the litter at 6 weeks by as much as 91 and $53\%$, respectively (P<0.05). $KMnO_4$ treatment decreased ammonia production at 6 weeks up to $69\%$ compared to the controls (P<0.05). Poultry litter amended with $AlCl_3\;+\;CaCO_3\;and\;KMnO_4$ also caused a decrease (P<0.01) in carbon dioxide productions at 6 weeks (59 and $65\%$, respectively). In conclusion, although broiler performance was not affected by the three chemical additives and control group, these results indicate that $FeSO_4,\;AlCl_3\;+\;CaCO_3\;and\;KMnO_4$ application to litter in a poultry house resulted in a significant reduction in atmospheric ammonia and carbon dioxide gas.
Park, Ku-Sung;Kim, Ho-Sub;Kong, Dong-Soo;Shin, Jae-Ki;Hwang, Soon-Jin
Korean Journal of Ecology and Environment
/
v.39
no.1
s.115
/
pp.100-109
/
2006
This study evaluated the effects of water velocity, substrates, and phosphorus concentrations on the growth of filamentous periphytic algae (FPA) in the two types of artificial channel systems using treated wastewater. Controlled parameters included 5 ${\sim}$ 15 cm $s^{-1}$ for the water velocity; 10 and 20 mm wire meshes, natural fiber net, gravel and tile for the substrates: and 0.05 ${\sim}$ 1.0 mgP $L^{-1}$ for the P concentration. Algal growth rate of FPA was compared using both chi. a and dry weight change with time. Under the controlled water velocity range, the growth of FPA increased with the velocity, but the maximum growth rate was shown in the velocity of 10 cm $s^{-1}$. The substrate that showed the maximum growth of FPA differed between the artificial channel and indoor channel, due to the influence of suspended matters which caused the clogging of the meshed substrates. Under the controled range of P concentration, the growth rates of all three FPA species (Spirogyra turfosa, Oedogonium fovelatum, Rhizoclonium riparium) increased with the P increase, but they showed the differential growth rates among different P concentrations. The results of this study suggest that under the circumstance having an large amount of nutrients FPA develop the biomass rapidly and that even a little increase over the threshold velocity causes the detachment of filamentous periphytic algae. Thus, FPA dynamics in eutrophic streams, such as those receiving treated wastewater, seem to be sensitive to the water velocity. On the other hand, detached algal filaments could deteriorate water quality and ecosystem function in receiving streams or down-stream, and thus they need to be recognized as an important factor in water quality management in eutrophic streams.
Journal of the Korean Society of Food Science and Nutrition
/
v.32
no.5
/
pp.739-744
/
2003
This study was carried out to investigate the antioxidative activity and to identify the active components of hot-water extract of Paeoniajaponica (PJ), which was a main ingredient of a herb mixture preparation recently established as a potent candidate of radioprotector in our laboratory. The water extract was fractionated with CHCl$_3$, EtOAc and n-BuOH. The extract and its fractions showed very low activity in hydroxyl radical scavenging test. In lipid peroxidation test, the extract, EtOAc and water fractions showed moderate inhibition with the ratio above 50%. In DPPH radical scavenging test, the extract, EtOAc and water fraction showed high activity with the ratio above 80%, especially. EtOAc fraction scavenged the radicals as much as synthetic antioxidant (BHA), even at low concentration. It is suggested that mai or partition for antioxidative activity of Paeonia japonica was EtOAc fraction. Subsequently, two active compounds (PJE021-1 and JE024-1) from EtOAc fraction were isolated by using MCI gel and silica gel column chromatography The two compounds inhibited remarkedly the $H_2O$$_2$-induced DNA damage in human peripheral blood lymphocytes, measured by single-cell gel electrophoresis (SCGE). PJE021-1 protected the cells to almost negative control level, dose-dependently. PJE024-1 exhibited a potent inhibition with the ratio of 71% at even low concentration (0.5 $\mu\textrm{g}$/$m\ell$). Finally, their chemical structures were identified as gallic acid (PJE021-1) and (+)-catechin (PJE024-1), respectively, on the basis of the speculation of spectral and physical data.
In this study, the atmospheric vertical structure (AVS) associated with summertime (June, July, and August) heavy rainfall in Seoul was classified into three patterns (Loaded Gun: L, Inverted V: IV, and Thin Tube: TT) using rawinsonde soundings launched at Osan from 2009 to 2018. The characteristics of classified AVS and precipitation property were analyzed. Occurrence frequencies in each type were 34.7% (TT-type), 20.4% (IV-type), 20.4% (LG-type), and 24.5% (Other-type), respectively. The mean value of Convective Available Potential Energy (1131.1 J kg-1) for LG-types and Storm Relative Helicity (357.6 ㎡s-2) for TT-types was about 2 times higher than that of other types, which seems to be the difference in the mechanism of convection at the low level atmosphere. The composited synoptic fields in all cases showed a pattern that warm and humid southwesterly wind flows into the Korean Peninsula. In the cases of TT-type, the low pressure center (at 850 hPa) was followed by the trough in upper-level (at 500 hPa) as the typical pattern of a low pressure deepening. The TT-type was strongly influenced by the low level jet (at 850 hPa), showing a pattern of connecting the upper- and low-level jets. The result of analysis indicated that precipitation was intensified in the first half of all types. IV-type precipitation induced by thermal instability tended to last for a short term period with strong precipitation intensity, while TT-type by mechanical instability showed weak precipitation over a long term period.
The present paper summerizes the studies on clay mineralogical characteristics of Korean soil, relationship between potassium and clay minerals, potassium release pattern of clay minerals and utilization of clay minerals for soil conditioner and fertilizers, which have been carried out in this laboratory. 1. The red yellow podzolic soil is mostly abundant in the upland of Korea and mainly consists of halloysite and weathered intermediates of mica such as illite and vermiculite. 2. With regard to soil parent material, kaolin mineral occurs abundant in soils derived from granite and granite gneiss. Mica is dominant in basaltic soil. The main clay mineral of the soil, originated from the Tertiary, is found montmorillonite and the volcanic soil of Jeju Island has plenty of allophane as its main clay mineral. 3. It is confirmed that the soil fertility depends on the composition of clay minerals. The red yellow podzolic soil, containing lot of kaolin, shows low productivity while the montmorillonite soil has higher productivity. 4. The release rate of solid phase potassium (micas and fixed potassium) follows the 1st order reaction equation in the equilibrium solution of $IN-NH_4OAc$. The potassium release constant is positively correlated with the mica content of the clay but negatively correlated with the content of $14.5{\AA}$ minerals. On the other hand, the potassium release constant has very high correlation with the ratio(Kex/Kt) of exchangeable potassium(Kex) to total potassium(Kt). 5. It is also found that Kex/Kt has rather high correlation with the content of mica and $14.5{\AA}$ minerals existed in the clay as well as the mica content of the soil.
This study was conducted to understand the influence of soil compaction on root growth and nutrient uptake characteristics of the soybean roots grown in two soils with different texture. Tap root elongation was measured on young seedling grown in cores compacted to different bulk densities of 1.2, 1.4 and $1.6/cm^3$ with different soil water retention in laboratory. The soil used were Samgag sandy loam and Baegsan loam soils. The wet and dry weight, total length, average radius and total surface area of roots were measured on soybean plants grown in 1/5000 a Wagner pots compacted to different bulk density of 1.2 and $1.4g/cm^3$. The nutrient uptake of soybean shoot was measured and evaluated with the unit surface area of roots at the 7th, 17th and 27th days after germination. The results were as follows: 1. The tap root elongation rate was faster in the loam soil with low bulk density than in the sandy loam soil with high bulk density. The elongation rates were remarkedly decreased when soil water was lower than the retention of 4 bars in loam soil and that of 1 bars in sandy loam soil. 2. Tap root elongation rate sharply decreased as increased soil strength higher than $2kgf/cm^2$ measured by ELE penetrometer showing curvillinear regression. However, it was low regardless of soil strength when soil water retention was 10 bars in sandy loam soil. 3. From the pot experiment, the total length of roots were longer in loam soil than in sandy loam soil and was longer in the soils with lower bulk density. The average radius of fine roots grown in sandy loam soil was larger than that grown in loam soil. The total surface area of roots was greater in the loam soil with low bulk density than in the sandy loam soil with high bulk density as the total length of roots. 4. The amounts of nutrient uptake by soybean shoots were greater in loam soil primarily due to more production of dry matter than in sandy loam soil. The nitrogen influx rates through the unit surface area were 597 to $753nmoles/day-cm^2$ in loam soil and 222 to $365nmoles/day\;cm^2$ in sandy loam soilshowing higher value in higher bulk density. The potasium influx rates were 99 to $175nmoles/day-cm^2$, and those of phosphate were 26 to $46nmoles/day\;cm^2$. Those of Ca and Mg were 175 to 246 and 163 to $205nmoles/day\;cm^2$. The difference in nutrient influx rates between bulk densities of these elements were lower than that of nitrogen.
In order to gain information on the plant characteristics of high yielding paddy from the point of view of mineral nutrition and to learn more about soil conditions under which high yields are produced a number of soil samples collected from the field experiment carried out in The City College of Seoul were analysed. In addition, a laboratory experiment carried out to support the field experiment was also analysed. Results obtained are as follows: 1. Generally, the higher the yield of paddy, the higher is the potassium content in flag leaves, whereas the reverse is true for the content of Ca and Mg in the leaves. From these facts it can conceivably be said that the high yielding paddy should absorb a large quantity of potassium and a lesser quantity of calcium and magnesium in the reproductive stage. 2. Leaves of paddy which produced yields over 600kg/10a (a comparatively high yield in the experiment) contained 30-35mg/100g of potassium, less than 25me/100g of calcium, and 10me/100g of magnesium which brings the ratio of $K/{\sqrt{Ca+Mg}}$ over 5. 3. Correlation studies indicated a remarkable relationship between the paddy yield and exchangeable potassium in soil samples taken after harvesting. A similar relationship was observed between the yield of paddy and $K/{\sqrt{Cae+Mge}}$ ratio of the soil samples. 4. A regression between yield of padcy (Y) and exchangeable potassium (Ke) or $K/{\sqrt{Ca+Mg}}$ in soil samples taken after harvesting was shown to be; Y=183.95+2135.86Ke $$Y=352.45+3114.454\;K/{\sqrt{Ca+Mg}}$$ 5. The regression between exchangeable potassium (Ke) in soil samples taken after harvesting and the amount of lime (L) and potassium (P) to be applied is; $$Ke=0.1246+0.00007037L+0.004444P+0.000004444L{\cdot}P$$.
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